HIV-1 Proviral DNA Excision Using an Evolved Recombinase (2007)
Sarkar, Indrani, Hauber, Ilona, Hauber, Joachim, Buchholz, Frank
HIV-1 integrates into the host chromosome and persists as a provirus flanked by long terminal repeats (LTRs). To date, treatment regimens primarily target the virus enzymes or virus-cell fusion, but...
Genome-scale RNAi profiling of cell division in human tissue culture cells (2007)
Kittler, Ralf, Pelletier, Laurence, Heninger, Anne-Kristine, Slabicki, Mikolaj, Theis, Mirko, Miroslaw, Lukasz, ...
Cell division is fundamental for all organisms. Here we report a genome-scale RNA-mediated interference screen in HeLa cells designed to identify human genes that are important for cell division. We...
Tandem affinity purification of functional TAP-tagged proteins from human cells (2007)
Gregan, Juraj, Riedel, Christian G., Petronczki, Mark, Cipak, Lubos, Rumpf, Cornelia, Poser, Ina, ...
Tandem affinity purification (TAP) is a generic two-step affinity purification protocol for isolation of TAP-tagged proteins together with associated proteins. We used bacterial artificial chromosome...
Cammenga, Jorg, Niebuhr, Birte, Horn, Stefan, Bergholz, Ulla, Putz, Gabriele, Buchholz, Frank, ...
Mutations in the RUNX1 gene are found at high frequencies in minimally differentiated acute myelogenous leukemia. In addition to null mutations, many of the mutations generate Runx1 DNA-binding (RDB)...
Molecular biology: chromosome guardians on duty (2006)
Riedel, Christian G., Katis, Vittorio L., Katou, Yuki, Mori, Saori, Itoh, Takehiko, Helmhart, Wolfgang, ...
Segregation of homologous maternal and paternal centromeres to opposite poles during meiosis I depends on post-replicative crossing over between homologous non-sister chromatids, which creates...
Minimizing the risk of reporting false positives in large-scale RNAi screens (2006)
Echeverri, Christophe J, Beachy, Philip A, Baum, Buzz, Boutros, Michael, Buchholz, Frank, Chanda, Sumit K, ...
Large-scale RNA interference (RNAi)-based analyses, very much as other 'omic' approaches, have inherent rates of false positives and negatives. The variability in the standards of care applied to...
RNAi in embryonic stem cells (2006)
Embryonic stem (ES) cells are pluripotent cells that can be isolated and grown in vitro from the inner cell mass of blastocysts. Their potential to differentiate into any cell of the body makes them...
AML1 deletion in adult mice causes splenomegaly and lymphomas (2006)
Putz, Gabriele, Rosner, Andrea, Nuesslein, Ina, Schmitz, Nicole, Buchholz, Frank
AML1 (RUNX1) encodes a DNA-binding subunit of the CBF transcription factor family and is required for the establishment of definitive hematopoiesis. AML1 is one of the most frequently mutated genes...
Enzymatically prepared RNAi libraries (2006)
Buchholz, Frank, Kittler, Ralf, Slabicki, Mikolaj, Theis, Mirko
Large-scale RNA interference (RNAi) screens in mammalian cells have mainly used synthetic small interfering RNA (siRNA) or short hairpin RNA (shRNA) libraries. The RNAi triggers for both of these...
RNAi Libraries for Functional Genomics (2006)
Heninger, Anne-Kristin, Kozak, Karol, Kittler, Ralf, Wagner, Jan, Lohmann, Annett, Poser, Ina, ...
Kittler,Ralf, Putz,Gabriele, Pelletier,Laurence, Poser,Ina, Heninger,Anne-Kristin, Drechsel,David, ...
RNA interference (RNAi) is an evolutionarily conserved defence mechanism whereby genes are specifically silenced through degradation of messenger RNAs; this process is mediated by homologous...
Kronke, Jan, Kittler, Ralf, Buchholz, Frank, Windisch, Marc P, Pietschmann, Thomas, Bartenschlager, Ralf, ...
Persistent infection with hepatitis C virus (HCV) is a leading cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. It has recently been shown that HCV RNA replication is...
DEQOR: a web-based tool for the design and quality control of siRNAs. (2004)
Henschel, Andreas, Buchholz, Frank, Habermann, Bianca
RNA interference (RNAi) is a powerful tool for inhibiting the expression of a gene by mediating the degradation of the corresponding mRNA. The basis of this gene-specific inhibition is small,...
Kittler, Ralf, Putz, Gabriele, Pelletier, Laurence, Poser, Ina, Heninger, Anne-Kristin, Drechsel, David, ...
RNA interference (RNAi) is an evolutionarily conserved defence mechanism whereby genes are specifically silenced through degradation of messenger RNAs; this process is mediated by homologous...
Calegari, Federico, Marzesco, Anne-Marie, Kittler, Ralf, Buchholz, Frank, Huttner, Wieland B
Abstract In mammals, embryonic development is more difficult to analyze than in non-mammalian species because this development occurs in utero. Interestingly, whole embryo culture allows the normal...
Kittler, Ralf, Putz, Gabriele, Pelletier, Laurence, Poser, Ina, Heninger, Anne-Kristin, Drechsel, David, ...
RNA interference (RNAi) is an evolutionarily conserved defence mechanism whereby genes are specifically silenced through degradation of messenger RNAs; this process is mediated by homologous...
RNA interference: gene silencing in the fast lane. (2003)
Kittler, Ralf, Buchholz, Frank
Sequencing of whole genomes has provided new perspectives into the blueprints of diverse organisms. Knowing the sequences, however, does not always tell us much about the function of the genes that...
Codon-improved Cre recombinase (iCre) expression in the mouse (2002)
Shimshek,Derya R., Kim,Jin-Hyun, Hübner,Michael R., Spergel,Daniel J., Buchholz,Frank, Casanova,James E., ...
By applying the mammalian codon usage to Cre recombinase, we improved Cre expression, as determined by immunoblot and functional analysis, in three different mammalian cell lines. The improved Cre...
Codon-improved Cre recombinase (iCre) expression in the mouse (2002)
Shimshek, Derya R., Kim, Jin-Hyun, Hübner, Michael R., Spergel, Daniel J., Buchholz, Frank, Casanova, James E., ...
By applying the mammalian codon usage to Cre recombinase, we improved Cre expression, as determined by immunoblot and functional analysis, in three different mammalian cell lines. The improved Cre...
Yang, Dun, Buchholz, Frank, Huang, Zhongdong, Goga, Andrei, Chen, Chih-Ying, Brodsky, Frances M., ...
Small interfering RNA (siRNA) has become a powerful tool for selectively silencing gene expression in cultured mammalian cells. Because different siRNAs of the same gene have variable silencing...
Codon-improved Cre recombinase (iCre) expression in the mouse (2002)
Shimshek, D. R., Kim, J., Hübner, M. R., Spergel, D. J., Buchholz, Frank, Casanova, E., ...
By applying the mammalian codon usage to Cre recombinase, we improved Cre expression, as determined by immunoblot and functional analysis, in three different mammalian cell lines. The improved Cre...
Calegari, Federico, Haubensak, Wulf, Yang, Dun, Huttner, Wieland B., Buchholz, Frank
RNA interference (RNAi) using double-stranded RNA has been used for the systematic analysis of gene function in invertebrate organisms. Here we have explored the use of short interfering RNA (siRNA)...
Thesis (doctoral)--Eberhard-Karls-Universität zu Tübingen, 1998.
Heidelberg, University, Diss., 1997.
Yang, Dun, Buchholz, Frank, Huang, Zhongdong, Goga, Andrei, Chen, Chih-Ying, Brodsky, Frances M., ...
Small interfering RNA (siRNA) has become a powerful tool for selectively silencing gene expression in cultured mammalian cells. Because different siRNAs of the same gene have variable silencing...
Calegari, Federico, Haubensak, Wulf, Yang, Dun, Huttner, Wieland B., Buchholz, Frank
RNA interference (RNAi) using double-stranded RNA has been used for the systematic analysis of gene function in invertebrate organisms. Here we have explored the use of short interfering RNA (siRNA)...
Ennifar, Eric, Meyer, Joachim E. W., Buchholz, Frank, Stewart, A. Francis, Suck, Dietrich
Escherichia coli phage P1 Cre recombinase catalyzes the site-specific recombination of DNA containing loxP sites. We report here two crystal structures of a wild-type Cre recombinase–loxP synaptic...
Krönke, Jan, Kittler, Ralf, Buchholz, Frank, Windisch, Marc P., Pietschmann, Thomas, Bartenschlager, Ralf, ...
Persistent infection with hepatitis C virus (HCV) is a leading cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. It has recently been shown that HCV RNA replication is...
DEQOR: a web-based tool for the design and quality control of siRNAs
Henschel, Andreas, Buchholz, Frank, Habermann, Bianca
RNA interference (RNAi) is a powerful tool for inhibiting the expression of a gene by mediating the degradation of the corresponding mRNA. The basis of this gene-specific inhibition is small,...
Kittler, Ralf, Pelletier, Laurence, Ma, Chunling, Poser, Ina, Fischer, Steffi, Hyman, Anthony A., ...
RNA interference (RNAi) is a widely used method for analysis of gene function in tissue culture cells. However, to date there has been no reliable method for testing the specificity of any particular...
Buchholz, Frank, Refaeli, Yosef, Trumpp, Andreas, Bishop, J. Michael
Transgenic mice have been used to explore the role of chromosomal translocations in the genesis of tumors. But none of these efforts has actually involved induction of a translocation in vivo. Here...
Yang, Dun, Buchholz, Frank, Huang, Zhongdong, Goga, Andrei, Chen, Chih-Ying, Brodsky, Frances M., ...
Small interfering RNA (siRNA) has become a powerful tool for selectively silencing gene expression in cultured mammalian cells. Because different siRNAs of the same gene have variable silencing...
Calegari, Federico, Haubensak, Wulf, Yang, Dun, Huttner, Wieland B., Buchholz, Frank
RNA interference (RNAi) using double-stranded RNA has been used for the systematic analysis of gene function in invertebrate organisms. Here we have explored the use of short interfering RNA (siRNA)...
Ennifar, Eric, Meyer, Joachim E. W., Buchholz, Frank, Stewart, A. Francis, Suck, Dietrich
Escherichia coli phage P1 Cre recombinase catalyzes the site-specific recombination of DNA containing loxP sites. We report here two crystal structures of a wild-type Cre recombinase–loxP synaptic...
Krönke, Jan, Kittler, Ralf, Buchholz, Frank, Windisch, Marc P., Pietschmann, Thomas, Bartenschlager, Ralf, ...
Persistent infection with hepatitis C virus (HCV) is a leading cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. It has recently been shown that HCV RNA replication is...
DEQOR: a web-based tool for the design and quality control of siRNAs
Henschel, Andreas, Buchholz, Frank, Habermann, Bianca
RNA interference (RNAi) is a powerful tool for inhibiting the expression of a gene by mediating the degradation of the corresponding mRNA. The basis of this gene-specific inhibition is small,...
Kittler, Ralf, Pelletier, Laurence, Ma, Chunling, Poser, Ina, Fischer, Steffi, Hyman, Anthony A., ...
RNA interference (RNAi) is a widely used method for analysis of gene function in tissue culture cells. However, to date there has been no reliable method for testing the specificity of any particular...
Buchholz, Frank, Refaeli, Yosef, Trumpp, Andreas, Bishop, J. Michael
Transgenic mice have been used to explore the role of chromosomal translocations in the genesis of tumors. But none of these efforts has actually involved induction of a translocation in vivo. Here...