Finbarr Hayes

A Family of Stability Determinants in Pathogenic Bacteria

Hayes, Finbarr

A novel segregational stability system was identified on plasmid R485, which originates from Morganella morganii. The system is composed of two overlapping genes, stbD and stbE, which potentially...

Identification of XcpZ Domains Required for Assembly of the Secreton of Pseudomonas aeruginosa

Robert, Viviane, Hayes, Finbarr, Lazdunski, Andrée, Michel, Gérard P. F.

Most of the exoproteins secreted by Pseudomonas aeruginosa are transported via the type II secretion system. This machinery, which is widely conserved in gram-negative bacteria, consists of 12 Xcp...

pAMβ1-Associated Mobilization of Proteinase Plasmids from Lactococcus lactis subsp. lactis UC317 and L. lactis subsp. cremoris UC205

Hayes, Finbarr, Caplice, Elizabeth, McSweeney, Anne, Fitzgerald, Gerald F., Daly, Charles

A combination of plasmid curing and DNA-DNA hybridization data facilitated the identification of proteinase plasmids of 75 (pCI301) and 35 kilobases (pCI203) in the multi-plasmid-containing strains...

Identification of the Minimal Replicon of Lactococcus lactis subsp. lactis UC317 Plasmid pCI305

Hayes, Finbarr, Daly, Charles, Fitzgerald, Gerald F.

Replication functions of the stable, cryptic 8.7-kilobase (kb) plasmid pCI305 from multi-plasmid-containing Lactococcus lactis subsp. lactis UC317 were studied. Analysis of this replicon was...

Protein Diversity Confers Specificity in Plasmid Segregation

Fothergill, Timothy J. G., Barillà, Daniela, Hayes, Finbarr

The ParG segregation protein (8.6 kDa) of multidrug resistance plasmid TP228 is a homodimeric DNA-binding factor. The ParG dimer consists of intertwined C-terminal domains that adopt a...

Bacterial DNA segregation dynamics mediated by the polymerizing protein ParF

Barillà, Daniela, Rosenberg, Mark F, Nobbmann, Ulf, Hayes, Finbarr

Prokaryotic DNA segregation most commonly involves members of the Walker-type ParA superfamily. Here we show that the ParF partition protein specified by the TP228 plasmid is a ParA ATPase that...

A Family of Stability Determinants in Pathogenic Bacteria

Hayes, Finbarr

A novel segregational stability system was identified on plasmid R485, which originates from Morganella morganii. The system is composed of two overlapping genes, stbD and stbE, which potentially...

Identification of XcpZ Domains Required for Assembly of the Secreton of Pseudomonas aeruginosa

Robert, Viviane, Hayes, Finbarr, Lazdunski, Andrée, Michel, Gérard P. F.

Most of the exoproteins secreted by Pseudomonas aeruginosa are transported via the type II secretion system. This machinery, which is widely conserved in gram-negative bacteria, consists of 12 Xcp...

pAMβ1-Associated Mobilization of Proteinase Plasmids from Lactococcus lactis subsp. lactis UC317 and L. lactis subsp. cremoris UC205

Hayes, Finbarr, Caplice, Elizabeth, McSweeney, Anne, Fitzgerald, Gerald F., Daly, Charles

A combination of plasmid curing and DNA-DNA hybridization data facilitated the identification of proteinase plasmids of 75 (pCI301) and 35 kilobases (pCI203) in the multi-plasmid-containing strains...

Identification of the Minimal Replicon of Lactococcus lactis subsp. lactis UC317 Plasmid pCI305

Hayes, Finbarr, Daly, Charles, Fitzgerald, Gerald F.

Replication functions of the stable, cryptic 8.7-kilobase (kb) plasmid pCI305 from multi-plasmid-containing Lactococcus lactis subsp. lactis UC317 were studied. Analysis of this replicon was...

Protein Diversity Confers Specificity in Plasmid Segregation

Fothergill, Timothy J. G., Barillà, Daniela, Hayes, Finbarr

The ParG segregation protein (8.6 kDa) of multidrug resistance plasmid TP228 is a homodimeric DNA-binding factor. The ParG dimer consists of intertwined C-terminal domains that adopt a...

Bacterial DNA segregation dynamics mediated by the polymerizing protein ParF

Barillà, Daniela, Rosenberg, Mark F, Nobbmann, Ulf, Hayes, Finbarr

Prokaryotic DNA segregation most commonly involves members of the Walker-type ParA superfamily. Here we show that the ParF partition protein specified by the TP228 plasmid is a ParA ATPase that...

Toxin–antitoxin regulation: bimodal interaction of YefM–YoeB with paired DNA palindromes exerts transcriptional autorepression

Kędzierska, Barbara, Lian, Lu-Yun, Hayes, Finbarr

Toxin–antitoxin (TA) complexes function in programmed cell death or stress response mechanisms in bacteria. The YefM–YoeB TA complex of Escherichia coli consists of YoeB toxin that is...

The tail of the ParG DNA segregation protein remodels ParF polymers and enhances ATP hydrolysis via an arginine finger-like motif

Barillà, Daniela, Carmelo, Emma, Hayes, Finbarr

The ParF protein of plasmid TP228 belongs to the ubiquitous superfamily of ParA ATPases that drive DNA segregation in bacteria. ATP-bound ParF polymerizes into multistranded filaments. The partner...

Centromere anatomy in the multidrug-resistant pathogen Enterococcus faecium

Derome, Andrew, Hoischen, Christian, Bussiek, Malte, Grady, Ruth, Adamczyk, Malgorzata, Kędzierska, Barbara, ...

Multidrug-resistant variants of the opportunistic human pathogen Enterococcus have recently emerged as leading agents of nosocomial infection. The acquisition of plasmid-borne resistance genes is a...

Genomic and Functional Characterization of the Modular Broad-Host-Range RA3 Plasmid, the Archetype of the IncU Group▿ †

Kulinska, Anna, Czeredys, Magdalena, Hayes, Finbarr, Jagura-Burdzy, Grazyna

IncU plasmids are a distinctive group of mobile elements with highly conserved backbone functions and variable antibiotic resistance gene cassettes. The IncU archetype is conjugative plasmid RA3,...