Fenyong Liu

Infection of human cytomegalovirus in cultured human gingival tissue (2006)

Hai, Rong, Chu, Alice, Li, Hongjian, Umamoto, Sean, Rider, Paul, Liu, Fenyong

Abstract Background Human cytomegalovirus (HCMV) infection in the oral cavity plays an important role in its horizontal transmission and in causing viral-associated oral diseases such as gingivitis....

Effective inhibition of human cytomegalovirus gene expression and replication by a ribozyme derived from the catalytic RNA subunit of RNase P from Escherichia coli

Trang, Phong, Lee, Manfred, Nepomuceno, Edward, Kim, Joe, Zhu, Hua, Liu, Fenyong

A sequence-specific ribozyme (M1GS RNA) derived from the catalytic RNA subunit of RNase P from Escherichia coli was used to target the overlapping exon 3 region of the mRNAs encoding the major...

RNase P-mediated inhibition of cytomegalovirus protease expression and viral DNA encapsidation by oligonucleotide external guide sequences

Dunn, Walter, Trang, Phong, Khan, Umair, Zhu, Jiaming, Liu, Fenyong

External guide sequences (EGSs) are oligonucleotides that consist of a sequence complementary to a target mRNA and recruit intracellular RNase P for specific degradation of the target RNA. In this...

Effective inhibition of herpes simplex virus 1 gene expression and growth by engineered RNase P ribozyme

Trang, Phong, Lee, Jarone, Kilani, Ahmed F., Kim, Joe, Liu, Fenyong

Using an in vitro selection procedure, we have previously isolated ribonuclease P (RNase P) ribozyme variants that efficiently cleave an mRNA sequence in vitro. In this study, an M1GS RNA variant was...

Differential effects of the protein cofactor on the interactions between an RNase P ribozyme and its target mRNA substrate

Hsu, Amy W., Kilani, Ahmed F., Liou, Kwa, Lee, Jarone, Liu, Fenyong

RNase P from Escherichia coli is a tRNA-processing enzyme and consists of a catalytic RNA subunit (M1 RNA) and a protein component (C5 protein). M1GS, a gene-targeting ribozyme derived from M1, can...

Construction and Characterization of Murine Cytomegaloviruses That Contain Transposon Insertions at Open Reading Frames m09 and M83

Zhan, Xiaoyan, Lee, Manfred, Xiao, Jianqiao, Liu, Fenyong

A transposon derived from Escherichia coli Tn3 was introduced into the genome of murine cytomegalovirus (MCMV) to generate a pool of viral mutants, including two recombinant viruses that contained...

In Vitro and In Vivo Characterization of a Murine Cytomegalovirus with a Transposon Insertional Mutation at Open Reading Frame M43

Xiao, Jianqiao, Tong, Tuong, Zhan, Xiaoyan, Haghjoo, Erik, Liu, Fenyong

We have recently generated a pool of murine cytomegalovirus (MCMV) mutants by using a Tn3-based transposon mutagenesis approach. In this study, one of the MCMV mutants, RvM43, which contained the...

Murine Cytomegalovirus Containing a Mutation at Open Reading Frame M37 Is Severely Attenuated in Growth and Virulence In Vivo

Lee, Manfred, Xiao, Jianqiao, Haghjoo, Erik, Zhan, Xiaoyan, Abenes, Gerry, Tuong, Tong, ...

A pool of murine cytomegalovirus (MCMV) mutants was generated by using a Tn3-based transposon mutagenesis procedure. One of the mutants, RvM37, which contained the transposon sequence at open reading...

Murine Cytomegalovirus Open Reading Frame M27 Plays an Important Role in Growth and Virulence in Mice

Abenes, Gerardo, Lee, Manfred, Haghjoo, Erik, Tong, Tuong, Zhan, Xiaoyan, Liu, Fenyong

Using a Tn3-based transposon mutagenesis approach, we have generated a pool of murine cytomegalovirus (MCMV) mutants. In this study, one of the mutants, RvM27, which contained the transposon sequence...

In Vitro and In Vivo Characterization of a Murine Cytomegalovirus with a Mutation at Open Reading Frame m166

Zhu, Jiaming, Chen, Jennifer, Hai, Rong, Tong, Tuong, Xiao, Jianqiao, Zhan, Xiaoyan, ...

We have recently generated a pool of murine cytomegalovirus (MCMV) mutants by using a Tn3-based transposon mutagenesis approach. In this study, one of the mutants, Rvm166, which contained the...

Murine Cytomegalovirus with a Transposon Insertional Mutation at Open Reading Frame M35 Is Defective in Growth In Vivo

Tam, Ada, Zhu, Jiaming, Hai, Rong, Haghjoo, Erik, Tong, Tuong, Zhan, Xiaoyan, ...

We had previously constructed a pool of murine cytomegalovirus (MCMV) mutants that contained a Tn3-based transposon sequence randomly inserted in the viral genome. In the study reported here, one of...

Functional profiling of a human cytomegalovirus genome

Dunn, Walter, Chou, Cassie, Li, Hong, Hai, Rong, Patterson, David, Stolc, Viktor, ...

Human cytomegalovirus (HCMV), a ubiquitous herpesvirus, causes a lifelong subclinical infection in healthy adults but leads to significant morbidity and mortality in neonates and immunocompromised...

Murine Cytomegalovirus with a Transposon Insertional Mutation at Open Reading Frame m155 Is Deficient in Growth and Virulence in Mice

Abenes, Gerardo, Chan, Karen, Lee, Manfred, Haghjoo, Erik, Zhu, Jiaming, Zhou, Tianhong, ...

A pool of murine cytomegalovirus (MCMV) mutants was previously generated by using a Tn3-based transposon mutagenesis approach (X. Zhan, M. Lee, J. Xiao, and F. Liu, J. Virol. 74:7411-7421, 2000). In...

Effective inhibition of Rta expression and lytic replication of Kaposi's sarcoma-associated herpesvirus by human RNase P

Zhu, Jiaming, Trang, Phong, Kim, Kihoon, Zhou, Tianhong, Deng, Hongyu, Liu, Fenyong

Ribonuclease P (RNase P) complexed with external guide sequence (EGS) represents a nucleic acid-based gene interference approach to knock-down gene expression. Unlike other strategies, such as...

RNase P ribozyme inhibits cytomegalovirus replication by blocking the expression of viral capsid proteins

Kim, Kihoon, Trang, Phong, Umamoto, Sean, Hai, Rong, Liu, Fenyong

By linking a guide sequence to the catalytic RNA subunit of RNase P (M1 RNA), we constructed a functional ribozyme (M1GS RNA) that targets the overlapping mRNA region of two human cytomegalovirus...

Molecular, Biological, and In Vivo Characterization of the Guinea Pig Cytomegalovirus (CMV) Homologs of the Human CMV Matrix Proteins pp71 (UL82) and pp65 (UL83)

McGregor, Alistair, Liu, Fenyong, Schleiss, Mark R.

We recently identified the genes encoding the guinea pig cytomegalovirus (GPCMV) homologs of the upper and lower matrix proteins of human CMV, pp71 (UL82) and pp65 (UL83), which we designated GP82...

Three-Dimensional Localization of the Smallest Capsid Protein in the Human Cytomegalovirus Capsid

Yu, Xuekui, Shah, Sanket, Atanasov, Ivo, Lo, Pierrette, Liu, Fenyong, Britt, William J., ...

The smallest capsid proteins (SCPs) of the human herpesviruses differ substantially in size and sequence and are thought to impart some unique aspects of infection to their respective viruses. We...

Two Gamma Interferon-Activated Site-Like Elements in the Human Cytomegalovirus Major Immediate-Early Promoter/Enhancer Are Important for Viral Replication†

Netterwald, James, Yang, Shaojun, Wang, Weijia, Ghanny, Salena, Cody, Michael, Soteropoulos, Patricia, ...

Human cytomegalovirus (HCMV) infection directly initiates a signal transduction pathway that leads to activation of a large number of cellular interferon-stimulated genes (ISGs). Our previous studies...

Dissecting human cytomegalovirus gene function and capsid maturation by ribozyme targeting and electron cryomicroscopy

Yu, Xuekui, Trang, Phong, Shah, Sanket, Atanasov, Ivo, Kim, Yong-Hwan, Bai, Yong, ...

Human CMV (HCMV) is the leading viral cause of birth defects and causes one of the most common opportunistic infections among transplant recipients and AIDS patients. Cleavage of internal scaffolding...

Engineered external guide sequences are highly effective in inducing RNase P for inhibition of gene expression and replication of human cytomegalovirus

Yang, Yong-Hua, Li, Hongjian, Zhou, Tianhong, Kim, Kihoon, Liu, Fenyong

External guide sequences (EGSs), which are RNA molecules derived from natural tRNAs, bind to a target mRNA and render the mRNA susceptible to hydrolysis by RNase P, a tRNA processing enzyme. Using an...

Intracellular expression of engineered RNase P ribozymes effectively blocks gene expression and replication of human cytomegalovirus

KIM, KIHOON, UMAMOTO, SEAN, TRANG, PHONG, HAI, RONG, LIU, FENYONG

A ribozyme (M1GS RNA) constructed from the catalytic RNA subunit of RNase P from Escherichia coli was used to target the overlapping region of two human cytomegalovirus (HCMV) mRNAs, which encode for...

Effective inhibition of human cytomegalovirus gene expression and growth by intracellular expression of external guide sequence RNA

LI, HONGJIAN, TRANG, PHONG, KIM, KIHOON, ZHOU, TIANHONG, UMAMOTO, SEAN, LIU, FENYONG

RNase P complexed with external guide sequence (EGS) represents a novel nucleic-acid-based gene interference approach to modulate gene expression. In this study, a functional EGS RNA was constructed...

Infection of human cytomegalovirus in cultured human gingival tissue

Hai, Rong, Chu, Alice, Li, Hongjian, Umamoto, Sean, Rider, Paul, Liu, Fenyong

Effective inhibition of human cytomegalovirus gene expression and replication by a ribozyme derived from the catalytic RNA subunit of RNase P from Escherichia coli

Trang, Phong, Lee, Manfred, Nepomuceno, Edward, Kim, Joe, Zhu, Hua, Liu, Fenyong

A sequence-specific ribozyme (M1GS RNA) derived from the catalytic RNA subunit of RNase P from Escherichia coli was used to target the overlapping exon 3 region of the mRNAs encoding the major...

RNase P-mediated inhibition of cytomegalovirus protease expression and viral DNA encapsidation by oligonucleotide external guide sequences

Dunn, Walter, Trang, Phong, Khan, Umair, Zhu, Jiaming, Liu, Fenyong

External guide sequences (EGSs) are oligonucleotides that consist of a sequence complementary to a target mRNA and recruit intracellular RNase P for specific degradation of the target RNA. In this...

Effective inhibition of herpes simplex virus 1 gene expression and growth by engineered RNase P ribozyme

Trang, Phong, Lee, Jarone, Kilani, Ahmed F., Kim, Joe, Liu, Fenyong

Using an in vitro selection procedure, we have previously isolated ribonuclease P (RNase P) ribozyme variants that efficiently cleave an mRNA sequence in vitro. In this study, an M1GS RNA variant was...

Differential effects of the protein cofactor on the interactions between an RNase P ribozyme and its target mRNA substrate

Hsu, Amy W., Kilani, Ahmed F., Liou, Kwa, Lee, Jarone, Liu, Fenyong

RNase P from Escherichia coli is a tRNA-processing enzyme and consists of a catalytic RNA subunit (M1 RNA) and a protein component (C5 protein). M1GS, a gene-targeting ribozyme derived from M1, can...

Construction and Characterization of Murine Cytomegaloviruses That Contain Transposon Insertions at Open Reading Frames m09 and M83

Zhan, Xiaoyan, Lee, Manfred, Xiao, Jianqiao, Liu, Fenyong

A transposon derived from Escherichia coli Tn3 was introduced into the genome of murine cytomegalovirus (MCMV) to generate a pool of viral mutants, including two recombinant viruses that contained...

In Vitro and In Vivo Characterization of a Murine Cytomegalovirus with a Transposon Insertional Mutation at Open Reading Frame M43

Xiao, Jianqiao, Tong, Tuong, Zhan, Xiaoyan, Haghjoo, Erik, Liu, Fenyong

We have recently generated a pool of murine cytomegalovirus (MCMV) mutants by using a Tn3-based transposon mutagenesis approach. In this study, one of the MCMV mutants, RvM43, which contained the...

Murine Cytomegalovirus Containing a Mutation at Open Reading Frame M37 Is Severely Attenuated in Growth and Virulence In Vivo

Lee, Manfred, Xiao, Jianqiao, Haghjoo, Erik, Zhan, Xiaoyan, Abenes, Gerry, Tuong, Tong, ...

A pool of murine cytomegalovirus (MCMV) mutants was generated by using a Tn3-based transposon mutagenesis procedure. One of the mutants, RvM37, which contained the transposon sequence at open reading...

Murine Cytomegalovirus Open Reading Frame M27 Plays an Important Role in Growth and Virulence in Mice

Abenes, Gerardo, Lee, Manfred, Haghjoo, Erik, Tong, Tuong, Zhan, Xiaoyan, Liu, Fenyong

Using a Tn3-based transposon mutagenesis approach, we have generated a pool of murine cytomegalovirus (MCMV) mutants. In this study, one of the mutants, RvM27, which contained the transposon sequence...

In Vitro and In Vivo Characterization of a Murine Cytomegalovirus with a Mutation at Open Reading Frame m166

Zhu, Jiaming, Chen, Jennifer, Hai, Rong, Tong, Tuong, Xiao, Jianqiao, Zhan, Xiaoyan, ...

We have recently generated a pool of murine cytomegalovirus (MCMV) mutants by using a Tn3-based transposon mutagenesis approach. In this study, one of the mutants, Rvm166, which contained the...

Murine Cytomegalovirus with a Transposon Insertional Mutation at Open Reading Frame M35 Is Defective in Growth In Vivo

Tam, Ada, Zhu, Jiaming, Hai, Rong, Haghjoo, Erik, Tong, Tuong, Zhan, Xiaoyan, ...

We had previously constructed a pool of murine cytomegalovirus (MCMV) mutants that contained a Tn3-based transposon sequence randomly inserted in the viral genome. In the study reported here, one of...

Functional profiling of a human cytomegalovirus genome

Dunn, Walter, Chou, Cassie, Li, Hong, Hai, Rong, Patterson, David, Stolc, Viktor, ...

Human cytomegalovirus (HCMV), a ubiquitous herpesvirus, causes a lifelong subclinical infection in healthy adults but leads to significant morbidity and mortality in neonates and immunocompromised...

Murine Cytomegalovirus with a Transposon Insertional Mutation at Open Reading Frame m155 Is Deficient in Growth and Virulence in Mice

Abenes, Gerardo, Chan, Karen, Lee, Manfred, Haghjoo, Erik, Zhu, Jiaming, Zhou, Tianhong, ...

A pool of murine cytomegalovirus (MCMV) mutants was previously generated by using a Tn3-based transposon mutagenesis approach (X. Zhan, M. Lee, J. Xiao, and F. Liu, J. Virol. 74:7411-7421, 2000). In...

Effective inhibition of Rta expression and lytic replication of Kaposi's sarcoma-associated herpesvirus by human RNase P

Zhu, Jiaming, Trang, Phong, Kim, Kihoon, Zhou, Tianhong, Deng, Hongyu, Liu, Fenyong

Ribonuclease P (RNase P) complexed with external guide sequence (EGS) represents a nucleic acid-based gene interference approach to knock-down gene expression. Unlike other strategies, such as...

RNase P ribozyme inhibits cytomegalovirus replication by blocking the expression of viral capsid proteins

Kim, Kihoon, Trang, Phong, Umamoto, Sean, Hai, Rong, Liu, Fenyong

By linking a guide sequence to the catalytic RNA subunit of RNase P (M1 RNA), we constructed a functional ribozyme (M1GS RNA) that targets the overlapping mRNA region of two human cytomegalovirus...

Molecular, Biological, and In Vivo Characterization of the Guinea Pig Cytomegalovirus (CMV) Homologs of the Human CMV Matrix Proteins pp71 (UL82) and pp65 (UL83)

McGregor, Alistair, Liu, Fenyong, Schleiss, Mark R.

We recently identified the genes encoding the guinea pig cytomegalovirus (GPCMV) homologs of the upper and lower matrix proteins of human CMV, pp71 (UL82) and pp65 (UL83), which we designated GP82...

Three-Dimensional Localization of the Smallest Capsid Protein in the Human Cytomegalovirus Capsid

Yu, Xuekui, Shah, Sanket, Atanasov, Ivo, Lo, Pierrette, Liu, Fenyong, Britt, William J., ...

The smallest capsid proteins (SCPs) of the human herpesviruses differ substantially in size and sequence and are thought to impart some unique aspects of infection to their respective viruses. We...

Two Gamma Interferon-Activated Site-Like Elements in the Human Cytomegalovirus Major Immediate-Early Promoter/Enhancer Are Important for Viral Replication†

Netterwald, James, Yang, Shaojun, Wang, Weijia, Ghanny, Salena, Cody, Michael, Soteropoulos, Patricia, ...

Human cytomegalovirus (HCMV) infection directly initiates a signal transduction pathway that leads to activation of a large number of cellular interferon-stimulated genes (ISGs). Our previous studies...

Dissecting human cytomegalovirus gene function and capsid maturation by ribozyme targeting and electron cryomicroscopy

Yu, Xuekui, Trang, Phong, Shah, Sanket, Atanasov, Ivo, Kim, Yong-Hwan, Bai, Yong, ...

Human CMV (HCMV) is the leading viral cause of birth defects and causes one of the most common opportunistic infections among transplant recipients and AIDS patients. Cleavage of internal scaffolding...

Engineered external guide sequences are highly effective in inducing RNase P for inhibition of gene expression and replication of human cytomegalovirus

Yang, Yong-Hua, Li, Hongjian, Zhou, Tianhong, Kim, Kihoon, Liu, Fenyong

External guide sequences (EGSs), which are RNA molecules derived from natural tRNAs, bind to a target mRNA and render the mRNA susceptible to hydrolysis by RNase P, a tRNA processing enzyme. Using an...

Effective inhibition of human cytomegalovirus gene expression and growth by intracellular expression of external guide sequence RNA

LI, HONGJIAN, TRANG, PHONG, KIM, KIHOON, ZHOU, TIANHONG, UMAMOTO, SEAN, LIU, FENYONG

RNase P complexed with external guide sequence (EGS) represents a novel nucleic-acid-based gene interference approach to modulate gene expression. In this study, a functional EGS RNA was constructed...

Intracellular expression of engineered RNase P ribozymes effectively blocks gene expression and replication of human cytomegalovirus

KIM, KIHOON, UMAMOTO, SEAN, TRANG, PHONG, HAI, RONG, LIU, FENYONG

A ribozyme (M1GS RNA) constructed from the catalytic RNA subunit of RNase P from Escherichia coli was used to target the overlapping region of two human cytomegalovirus (HCMV) mRNAs, which encode for...

Infection of human cytomegalovirus in cultured human gingival tissue

Hai, Rong, Chu, Alice, Li, Hongjian, Umamoto, Sean, Rider, Paul, Liu, Fenyong

The Cytomegalovirus m155 Gene Product Subverts Natural Killer Cell Antiviral Protection by Disruption of H60–NKG2D Interactions

Lodoen, Melissa B., Abenes, Gerardo, Umamoto, Sean, Houchins, Jeffrey P., Liu, Fenyong, Lanier, Lewis L.

Natural killer (NK) cells are an important early mediator of host immunity to murine cytomegalovirus (MCMV) infection. However, MCMV has evolved mechanisms to elude recognition and clearance by NK...