A real-time view of the TAR:Tat:P-TEFb complex at HIV-1 transcription sites (2007)
Molle, Dorothée, Maiuri, Paolo, Boireau, Stéphanie, Bertrand, Edouard, Knezevich, Anna, Marcello, Alessandro, ...
Abstract HIV-1 transcription is tightly regulated: silent in long-term latency and highly active in acutely-infected cells. Transcription is activated by the viral protein Tat, which recruits the...
Cell polarity and intracellular localization of actin mRNA (2004)
Lavoie, Brigitte, Basyuk, Eugenia, Bordonné, Rémy, Bertrand, Édouard
In many species, intracellular mRNA localization is linked to cell polarity. In many cases however, mRNAs become localized as a result of a pre-existing cell-polarity, and they do not modify it....
Alkaline fixation drastically improves the signal of in situ hybridization
Basyuk, Eugenia, Bertrand, Edouard, Journot, Laurent
In situ hybridization (ISH) is widely used to detect DNA and RNA sequences within the cell and tissue sections. The important step in performing this technique is tissue fixation. We investigated the...
Human let-7 stem–loop precursors harbor features of RNase III cleavage products
Basyuk, Eugenia, Suavet, Florence, Doglio, Alain, Bordonné, Rémy, Bertrand, Edouard
The bidentate RNase III Dicer cleaves microRNA precursors to generate the 21–23 nt long mature RNAs. These precursors are 60–80 nt long, they fold into a characteristic stem–loop structure and...
Alkaline fixation drastically improves the signal of in situ hybridization
Basyuk, Eugenia, Bertrand, Edouard, Journot, Laurent
In situ hybridization (ISH) is widely used to detect DNA and RNA sequences within the cell and tissue sections. The important step in performing this technique is tissue fixation. We investigated the...
Human let-7 stem–loop precursors harbor features of RNase III cleavage products
Basyuk, Eugenia, Suavet, Florence, Doglio, Alain, Bordonné, Rémy, Bertrand, Edouard
The bidentate RNase III Dicer cleaves microRNA precursors to generate the 21–23 nt long mature RNAs. These precursors are 60–80 nt long, they fold into a characteristic stem–loop structure and...
A real-time view of the TAR:Tat:P-TEFb complex at HIV-1 transcription sites
Molle, Dorothée, Maiuri, Paolo, Boireau, Stéphanie, Bertrand, Edouard, Knezevich, Anna, Marcello, Alessandro, ...
HIV-1 transcription is tightly regulated: silent in long-term latency and highly active in acutely-infected cells. Transcription is activated by the viral protein Tat, which recruits the elongation...
The Clathrin Adaptor Complex AP-1 Binds HIV-1 and MLV Gag and Facilitates Their Budding
Camus, Grégory, Segura-Morales, Carolina, Molle, Dorothee, Lopez-Vergès, Sandra, Begon-Pescia, Christina, Cazevieille, Chantal, ...
Retroviral assembly is driven by Gag, and nascent viral particles escape cells by recruiting the machinery that forms intralumenal vesicles of multivesicular bodies. In this study, we show that the...
Bsr, a Nuclear-retained RNA with Monoallelic Expression
Royo, Hélène, Basyuk, Eugenia, Marty, Virginie, Marques, Maud, Bertrand, Edouard, Cavaillé, Jérôme
The imprinted Dlk1-Gtl2 and Prader-Willi syndrome (PWS) regions are characterized by a complex noncoding transcription unit spanning arrays of tandemly repeated C/D RNA genes. These noncoding RNAs...
ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs
Vitali, Patrice, Basyuk, Eugenia, Le Meur, Elodie, Bertrand, Edouard, Muscatelli, Françoise, Cavaillé, Jérôme, ...
Posttranscriptional, site-specific adenosine to inosine (A-to-I) base conversions, designated as RNA editing, play significant roles in generating diversity of gene expression. However, little is...
The transcriptional cycle of HIV-1 in real-time and live cells
Boireau, Stéphanie, Maiuri, Paolo, Basyuk, Eugenia, De La Mata, Manuel, Knezevich, Anna, Pradet-Balade, Bérangère, ...
RNA polymerase II (RNAPII) is a fundamental enzyme, but few studies have analyzed its activity in living cells. Using human immunodeficiency virus (HIV) type 1 reporters, we study real-time messenger...