Ernesto García

Pneumococcal LytA Autolysin, a Potent Therapeutic Agent in Experimental Peritonitis-Sepsis Caused by Highly β-Lactam-Resistant Streptococcus pneumoniae (2007)

Soriano, Francisco, Rodríguez-Cerrato, Violeta, García, Pedro, Huelves, Lorena, García, Ernesto, Prado, Gema Del, ...

The in vitro and in vivo antipneumococcal activities of the main pneumococcal autolysin (LytA) and Cpl-1, a lysozyme encoded by phage Cp-1, were studied. Intraperitoneal therapy with LytA or...

Perderse en la universidad: ausencias en las evaluaciones de primer curso en la Universitat de València (2007)

Villar, Alícia, García, Ernesto

Una parte significativa de los/las estudiantes que se matriculan de primer curso en la Universitat de València no llega a someterse a las correspondientes evaluaciones o lo hace sólo para un...

El maestro es el guardián de la utopía (2002)

García, Ernesto

Hace ya muchas generaciones que los maestros hemos perdido la idea del bien común por cuyo motivo el ejercicio docente ha perdido el horizonte vocacional que en principio le es inherente. Por...

El maestro es el guardián de la utopía (2002)

García, Ernesto

Hace ya muchas generaciones que los maestros hemos perdido la idea del bien común por cuyo motivo el ejercicio docente ha perdido el horizonte vocacional que en principio le es inherente. Por...

Armando un cuento : una propuesta de educación para el desarrollo con niños y niñas (1998)

Masino, Diana, Vázquez, José, Belmonte, Cristina, García, Ernesto, Hernández, Concepción, Romero, Miguel

Unidad didáctica propuesta para la educación para el desarrollo con niños y niñas de 6 a 10 años de edad a través del recurso metodológico del cuento, p. 39

Reducción del impacto del estrés y prevención de déficits asociados al envejecimiento en ratas: la estimulación postnatal y el enriquecimiento ambiental (1994)

Ferré Romeu, Pilar, Escorihuela, Rosa María, Fernández-Teruel, Alberto, Núñez, Joan Francesc, García, Ernesto, González, Berta, ...

Ante situaciones de alarma (o estrés), los organismos desencadenan una serie de reacciones fisiológicas que, si bien son adaptativas, pueden producir a largo plazo efectos nocivos que se relacionan...

Characterization of the Type 8 Capsular Gene Cluster of Streptococcus pneumoniae

Muñoz, Rosario, Mollerach, Marta, López, Rubens, García, Ernesto

The complete nucleotide sequence of the capsular gene cluster (cap8) responsible for the biosynthesis of the capsular polysaccharide of Streptococcus pneumoniae type 8 has been determined. The cap8...

Characterization of IS1515, a Functional Insertion Sequence in Streptococcus pneumoniae

Muñoz, Rosario, López, Rubens, García, Ernesto

We describe the characterization of a new insertion sequence, IS1515, identified in the genome of Streptococcus pneumoniae I41R, an unencapsulated mutant isolated many years ago (R. Austrian, H. P....

Molecular Peculiarities of the lytA Gene Isolated from Clinical Pneumococcal Strains That Are Bile Insoluble

Obregón, Virginia, García, Pedro, García, Ernesto, Fenoll, Asunción, López, Rubens, García, José L.

The autolytic LytA amidase from 12 bile (deoxycholate)-insoluble streptococcal isolates (formerly classified as atypical Streptococcus pneumoniae) showing different antibiotic resistance patterns was...

Genome Organization and Molecular Analysis of the Temperate Bacteriophage MM1 of Streptococcus pneumoniae

Obregón, Virginia, García, José L., García, Ernesto, López, Rubens, García, Pedro

The genome of MM1 (40,248 bp), a temperate bacteriophage from the Spain23F-1 multiresistant epidemic clone of Streptococcus pneumoniae, is organized in 53 open reading frames (ORFs) and in at least...

Characterization of LytA-Like N-Acetylmuramoyl-l-Alanine Amidases from Two New Streptococcus mitis Bacteriophages Provides Insights into the Properties of the Major Pneumococcal Autolysin

Romero, Patricia, López, Rubens, García, Ernesto

Two new temperate bacteriophages exhibiting a Myoviridae (φB6) and a Siphoviridae (φHER) morphology have been isolated from Streptococcus mitis strains B6 and HER 1055, respectively, and partially...

Implications of Physiological Studies Based on Genomic Sequences: Streptococcus pneumoniae TIGR4 Synthesizes a Functional LytC Lysozyme

Moscoso, Miriam, López, Elena, García, Ernesto, López, Rubens

The Streptococcus pneumoniae LytC lysozyme is responsible for autolysis at 30°C (a temperature close to that of the upper respiratory tract), promotes DNA release in competent cultures, and...

Allelic Variation of Polymorphic Locus lytB, Encoding a Choline-Binding Protein, from Streptococci of the Mitis Group

Moscoso, Miriam, Obregón, Virginia, López, Rubens, García, José L., García, Ernesto

The choline-binding protein LytB, an N-acetylglucosaminidase of Streptococcus pneumoniae, is the key enzyme for daughter cell separation and is believed to play a critical pathogenic role,...

Characteristic Signatures of the lytA Gene Provide a Basis for Rapid and Reliable Diagnosis of Streptococcus pneumoniae Infections†

Llull, Daniel, López, Rubens, García, Ernesto

The nucleotide sequences of the lytA gene from 29 pneumococcal isolates of various serotypes and 22 additional streptococci of the mitis group (including two Streptococcus pseudopneumoniae strains)...

Characterization of the Type 8 Capsular Gene Cluster of Streptococcus pneumoniae

Muñoz, Rosario, Mollerach, Marta, López, Rubens, García, Ernesto

The complete nucleotide sequence of the capsular gene cluster (cap8) responsible for the biosynthesis of the capsular polysaccharide of Streptococcus pneumoniae type 8 has been determined. The cap8...

Characterization of IS1515, a Functional Insertion Sequence in Streptococcus pneumoniae

Muñoz, Rosario, López, Rubens, García, Ernesto

We describe the characterization of a new insertion sequence, IS1515, identified in the genome of Streptococcus pneumoniae I41R, an unencapsulated mutant isolated many years ago (R. Austrian, H. P....

Molecular Peculiarities of the lytA Gene Isolated from Clinical Pneumococcal Strains That Are Bile Insoluble

Obregón, Virginia, García, Pedro, García, Ernesto, Fenoll, Asunción, López, Rubens, García, José L.

The autolytic LytA amidase from 12 bile (deoxycholate)-insoluble streptococcal isolates (formerly classified as atypical Streptococcus pneumoniae) showing different antibiotic resistance patterns was...

Genome Organization and Molecular Analysis of the Temperate Bacteriophage MM1 of Streptococcus pneumoniae

Obregón, Virginia, García, José L., García, Ernesto, López, Rubens, García, Pedro

The genome of MM1 (40,248 bp), a temperate bacteriophage from the Spain23F-1 multiresistant epidemic clone of Streptococcus pneumoniae, is organized in 53 open reading frames (ORFs) and in at least...

Characterization of LytA-Like N-Acetylmuramoyl-l-Alanine Amidases from Two New Streptococcus mitis Bacteriophages Provides Insights into the Properties of the Major Pneumococcal Autolysin

Romero, Patricia, López, Rubens, García, Ernesto

Two new temperate bacteriophages exhibiting a Myoviridae (φB6) and a Siphoviridae (φHER) morphology have been isolated from Streptococcus mitis strains B6 and HER 1055, respectively, and partially...

Implications of Physiological Studies Based on Genomic Sequences: Streptococcus pneumoniae TIGR4 Synthesizes a Functional LytC Lysozyme

Moscoso, Miriam, López, Elena, García, Ernesto, López, Rubens

The Streptococcus pneumoniae LytC lysozyme is responsible for autolysis at 30°C (a temperature close to that of the upper respiratory tract), promotes DNA release in competent cultures, and...

Allelic Variation of Polymorphic Locus lytB, Encoding a Choline-Binding Protein, from Streptococci of the Mitis Group

Moscoso, Miriam, Obregón, Virginia, López, Rubens, García, José L., García, Ernesto

The choline-binding protein LytB, an N-acetylglucosaminidase of Streptococcus pneumoniae, is the key enzyme for daughter cell separation and is believed to play a critical pathogenic role,...

Characteristic Signatures of the lytA Gene Provide a Basis for Rapid and Reliable Diagnosis of Streptococcus pneumoniae Infections†

Llull, Daniel, López, Rubens, García, Ernesto

The nucleotide sequences of the lytA gene from 29 pneumococcal isolates of various serotypes and 22 additional streptococci of the mitis group (including two Streptococcus pseudopneumoniae strains)...

Biofilm Formation by Streptococcus pneumoniae: Role of Choline, Extracellular DNA, and Capsular Polysaccharide in Microbial Accretion▿

Moscoso, Miriam, García, Ernesto, López, Rubens

Streptococcus pneumoniae colonizes the human upper respiratory tract, and this asymptomatic colonization is known to precede pneumococcal disease. In this report, chemically defined and semisynthetic...

Molecular Characterization of Disease-Associated Streptococci of the Mitis Group That Are Optochin Susceptible▿

Balsalobre, Luz, Hernández-Madrid, Antonia, Llull, Daniel, Martín-Galiano, Antonio J., García, Ernesto, Fenoll, Asunción, ...

Eight optochin-susceptible (Opts) alpha-hemolytic (viridans) streptococcus isolates were characterized at the molecular level. These isolates showed phenotypic characteristics typical of both...

In Vitro Bactericidal Activity of the Antiprotozoal Drug Miltefosine against Streptococcus pneumoniae and Other Pathogenic Streptococci▿

Llull, Daniel, Rivas, Luis, García, Ernesto

Miltefosine (hexadecylphosphocholine), the first oral drug against visceral leishmaniasis, triggered pneumococcal autolysis at concentrations higher than 2.5 μM. Bactericidal activity was also...

Pneumococcal LytA Autolysin, a Potent Therapeutic Agent in Experimental Peritonitis-Sepsis Caused by Highly β-Lactam-Resistant Streptococcus pneumoniae▿

Rodríguez-Cerrato, Violeta, García, Pedro, Huelves, Lorena, García, Ernesto, Del Prado, Gema, Gracia, Matilde, ...

The in vitro and in vivo antipneumococcal activities of the main pneumococcal autolysin (LytA) and Cpl-1, a lysozyme encoded by phage Cp-1, were studied. Intraperitoneal therapy with LytA or...

A Single Gene (tts) Located outside the cap Locus Directs the Formation of Streptococcus pneumoniae Type 37 Capsular Polysaccharide: Type 37 Pneumococci Are Natural, Genetically Binary Strains

Llull, Daniel, Muñoz, Rosario, López, Rubens, García, Ernesto

The molecular aspects of the type 37 pneumococcal capsular biosynthesis, a homopolysaccharide composed of sophorosyl units (β-d-Glc-(1→2)-β-d-Glc) linked by β-1,3 bonds, have been studied....

Characterization of the galU Gene of Streptococcus pneumoniae Encoding a Uridine Diphosphoglucose Pyrophosphorylase: A Gene Essential for Capsular Polysaccharide Biosynthesis

Mollerach, Marta, López, Rubens, García, Ernesto

The galU gene of Streptococcus pneumoniae has been cloned and sequenced. Escherichia coli cells harboring the recombinant plasmid pMMG2 (galU) overproduced a protein that has been shown to correspond...

Mutations in the tacF Gene of Clinical Strains and Laboratory Transformants of Streptococcus pneumoniae: Impact on Choline Auxotrophy and Growth Rate▿ †

González, Ana, Llull, Daniel, Morales, María, García, Pedro, García, Ernesto

The nutritional requirement that Streptococcus pneumoniae has for the aminoalcohol choline as a component of teichoic and lipoteichoic acids appears to be exclusive to this prokaryote. A mutation in...