Soupene, Eric, Kuypers, Frans A
Abstract Background The formation of acyl-CoA by the action of acyl-CoA synthetases plays a crucial role in membrane lipid turnover, including the plasma membrane of erythrocytes. In human, five...
Zimmer, Daniel P., Soupene, Eric, Lee, Haidy L., Wendisch, Volker F., Khodursky, Arkady B., Peter, Brian J., ...
Nitrogen regulatory protein C (NtrC) of enteric bacteria activates transcription of genes/operons whose products minimize the slowing of growth under nitrogen-limiting conditions. To reveal the NtrC...
Soupene, Eric, He, Luhong, Yan, Dalai, Kustu, Sydney
Homologues of the amtB gene of enteric bacteria exist in all three domains of life. Although their products are required for transport of the ammonium analogue methylammonium in washed cells, only in...
Soupene, Eric, Ramirez, Robert M., Kustu, Sydney
Methylammonium and ammonium (MEP) permeases of Saccharomyces cerevisiae belong to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+ + NH3). Transport and...
He, Luhong, Soupene, Eric, Ninfa, Alexander, Kustu, Sydney
In Klebsiella pneumoniae, NifA-dependent transcription of nitrogen fixation (nif) genes is inhibited by a flavoprotein, NifL, in the presence of molecular oxygen and/or combined nitrogen. We recently...
Ammonium/methylammonium transport (Amt) proteins facilitate diffusion of NH3 bidirectionally
Soupene, Eric, Lee, Haidy, Kustu, Sydney
The ammonium/methylammonium transport (Amt) proteins of enteric bacteria and their homologues, the methylammonium/ammonium permeases of Saccharomyces cerevisiae, are required for fast growth at very...
Rhesus expression in a green alga is regulated by CO2
Soupene, Eric, King, Natalie, Feild, Eithne, Liu, Phillip, Niyogi, Krishna K., Huang, Cheng-Han, ...
The function of the Rhesus (Rh) complex in the human red cell membrane has been unknown for six decades. Based on the organismal, organ, and tissue distribution of Rh proteins, and on our evidence...
Gas Channels for NH3: Proteins from Hyperthermophiles Complement an Escherichia coli Mutant
Soupene, Eric, Chu, Tony, Corbin, Rebecca W., Hunt, Donald F., Kustu, Sydney
Ammonium transport (Amt) proteins appear to be bidirectional channels for NH3. The amt genes of the hyperthermophiles Aquifex aeolicus and Methanococcus jannaschii complement enteric amtB mutants for...
Aquaporin Z of Escherichia coli: Reassessment of Its Regulation and Physiological Role
Soupene, Eric, King, Natalie, Lee, Haidy, Kustu, Sydney
Transcription of an aqpZ-lac fusion in a single copy on the Escherichia coli chromosome increased as cells entered the stationary growth phase. This was true in a variety of media, and increased...
Toward a protein profile of Escherichia coli: Comparison to its transcription profile
Corbin, Rebecca W., Paliy, Oleg, Yang, Feng, Shabanowitz, Jeffrey, Platt, Mark, Lyons, Charles E., ...
High-pressure liquid chromatography–tandem mass spectrometry was used to obtain a protein profile of Escherichia coli strain MG1655 grown in minimal medium with glycerol as the carbon source. By...
Soupene, Eric, Van Heeswijk, Wally C., Plumbridge, Jacqueline, Stewart, Valley, Bertenthal, Daniel, Lee, Haidy, ...
Escherichia coli strain MG1655 was chosen for sequencing because the few mutations it carries (ilvG rfb-50 rph-1) were considered innocuous. However, it has a number of growth defects. Internal...
Soupene, Eric, Inwood, William, Kustu, Sydney
Although Rhesus (Rh) proteins are best known as antigens on human red blood cells, they are not restricted to red cells or to mammals, and hence their primary biochemical functions can be studied in...
Zimmer, Daniel P., Soupene, Eric, Lee, Haidy L., Wendisch, Volker F., Khodursky, Arkady B., Peter, Brian J., ...
Nitrogen regulatory protein C (NtrC) of enteric bacteria activates transcription of genes/operons whose products minimize the slowing of growth under nitrogen-limiting conditions. To reveal the NtrC...
Soupene, Eric, He, Luhong, Yan, Dalai, Kustu, Sydney
Homologues of the amtB gene of enteric bacteria exist in all three domains of life. Although their products are required for transport of the ammonium analogue methylammonium in washed cells, only in...
Soupene, Eric, Ramirez, Robert M., Kustu, Sydney
Methylammonium and ammonium (MEP) permeases of Saccharomyces cerevisiae belong to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+ + NH3). Transport and...
He, Luhong, Soupene, Eric, Ninfa, Alexander, Kustu, Sydney
In Klebsiella pneumoniae, NifA-dependent transcription of nitrogen fixation (nif) genes is inhibited by a flavoprotein, NifL, in the presence of molecular oxygen and/or combined nitrogen. We recently...
Ammonium/methylammonium transport (Amt) proteins facilitate diffusion of NH3 bidirectionally
Soupene, Eric, Lee, Haidy, Kustu, Sydney
The ammonium/methylammonium transport (Amt) proteins of enteric bacteria and their homologues, the methylammonium/ammonium permeases of Saccharomyces cerevisiae, are required for fast growth at very...
Rhesus expression in a green alga is regulated by CO2
Soupene, Eric, King, Natalie, Feild, Eithne, Liu, Phillip, Niyogi, Krishna K., Huang, Cheng-Han, ...
The function of the Rhesus (Rh) complex in the human red cell membrane has been unknown for six decades. Based on the organismal, organ, and tissue distribution of Rh proteins, and on our evidence...
Gas Channels for NH3: Proteins from Hyperthermophiles Complement an Escherichia coli Mutant
Soupene, Eric, Chu, Tony, Corbin, Rebecca W., Hunt, Donald F., Kustu, Sydney
Ammonium transport (Amt) proteins appear to be bidirectional channels for NH3. The amt genes of the hyperthermophiles Aquifex aeolicus and Methanococcus jannaschii complement enteric amtB mutants for...
Aquaporin Z of Escherichia coli: Reassessment of Its Regulation and Physiological Role
Soupene, Eric, King, Natalie, Lee, Haidy, Kustu, Sydney
Transcription of an aqpZ-lac fusion in a single copy on the Escherichia coli chromosome increased as cells entered the stationary growth phase. This was true in a variety of media, and increased...
Toward a protein profile of Escherichia coli: Comparison to its transcription profile
Corbin, Rebecca W., Paliy, Oleg, Yang, Feng, Shabanowitz, Jeffrey, Platt, Mark, Lyons, Charles E., ...
High-pressure liquid chromatography–tandem mass spectrometry was used to obtain a protein profile of Escherichia coli strain MG1655 grown in minimal medium with glycerol as the carbon source. By...
Soupene, Eric, Van Heeswijk, Wally C., Plumbridge, Jacqueline, Stewart, Valley, Bertenthal, Daniel, Lee, Haidy, ...
Escherichia coli strain MG1655 was chosen for sequencing because the few mutations it carries (ilvG rfb-50 rph-1) were considered innocuous. However, it has a number of growth defects. Internal...
Soupene, Eric, Inwood, William, Kustu, Sydney
Although Rhesus (Rh) proteins are best known as antigens on human red blood cells, they are not restricted to red cells or to mammals, and hence their primary biochemical functions can be studied in...
Mammalian acyl-CoA:lysophosphatidylcholine acyltransferase enzymes
Soupene, Eric, Fyrst, Henrik, Kuypers, Frans A.
The mammalian RBC lacks de novo lipid synthesis but maintains its membrane composition by rapid turnover of acyl moieties at the sn-2 position of phospholipids. Plasma-derived fatty acids are...