Eric C. Holland

Identification of a multipotent astrocytic stem cell in the immature and adult mouse brain

Laywell, Eric D., Rakic, Pasko, Kukekov, Valery G., Holland, Eric C., Steindler, Dennis A.

The mammalian brain contains a population of neural stem cells (NSC) that can both self-renew and generate progeny along the three lineage pathways of the central nervous system (CNS), but the in...

Basic fibroblast growth factor induces cell migration and proliferation after glia-specific gene transfer in mice

Holland, Eric C., Varmus, Harold E.

Basic fibroblast growth factor (bFGF) is overexpressed in most high-grade human gliomas, implying that it is involved in the pathogenesis of these tumors. To assess the biological effect of...

Dissection of the c-Kit signaling pathway in mouse primordial germ cells by retroviral-mediated gene transfer

De Miguel, Maria P., Cheng, Linzhao, Holland, Eric C., Federspiel, Mark J., Donovan, Peter J.

Establishment of the mammalian germ line is a prerequisite for fertility of the adult animal but we know surprisingly little about the molecular mechanisms regulating germ-line development in...

p190RhoGAP can act to inhibit PDGF-induced gliomas in mice: a putative tumor suppressor encoded on human Chromosome 19q13.3

Wolf, Rebecca M., Draghi, Nicole, Liang, Xiquan, Dai, Chengkai, Uhrbom, Lene, Eklöf, Charlotta, ...

p190RhoGAP and Rho are key regulators of oligodendrocyte differentiation. The gene encoding p190RhoGAP is located at 19q13.3 of the human chromosome, a locus that is deleted in 50%–80% of...

A constitutively active epidermal growth factor receptor cooperates with disruption of G1 cell-cycle arrest pathways to induce glioma-like lesions in mice

Holland, Eric C., Hively, Wendy P., DePinho, Ronald A., Varmus, Harold E.

The epidermal growth factor receptor (EGFR) gene is amplified or mutated in 30%–50% of human gliobastoma multiforme (GBM). These mutations are associated usually with deletions of the INK4a–ARF...

Modeling mutations in the G1 arrest pathway in human gliomas: overexpression of CDK4 but not loss of INK4a–ARF induces hyperploidy in cultured mouse astrocytes

Holland, Eric C., Hively, Wendy P., Gallo, Vittorio, Varmus, Harold E.

Nearly all human gliomas exhibit alterations in one of three genetic loci governing G1 arrest: INK4a–ARF, CDK4, or RB. To discern the roles of CDK4 amplification and INK4a–ARF loss in...

Differential exoprotease activities confer tumor-specific serum peptidome patterns

Villanueva, Josep, Shaffer, David R., Philip, John, Chaparro, Carlos A., Erdjument-Bromage, Hediye, Olshen, Adam B., ...

Recent studies have established distinctive serum polypeptide patterns through mass spectrometry (MS) that reportedly correlate with clinically relevant outcomes. Wider acceptance of these signatures...

PDGF autocrine stimulation dedifferentiates cultured astrocytes and induces oligodendrogliomas and oligoastrocytomas from neural progenitors and astrocytes in vivo

Dai, Chengkai, Celestino, Joseph C., Okada, Yoshifumi, Louis, David N., Fuller, Greory N., Holland, Eric C.

We present evidence that some low-grade oligodendrogliomas may be comprised of proliferating glial progenitor cells that are blocked in their ability to differentiate, whereas malignant gliomas have...

The Characteristics of Astrocytomas and Oligodendrogliomas Are Caused by Two Distinct and Interchangeable Signaling Formats1

Dai, Chengkai, Lyustikman, Yelena, Shih, Alan, Hu, Xiaoyi, Fuller, Gregory N, Rosenblum, Marc, ...

Chronic platelet-derived growth factor (PDGF) signaling in glial progenitors leads to the formation of oligodendrogliomas in mice, whereas chronic combined Ras and Akt signaling leads to...

mTOR Promotes Survival and Astrocytic Characteristics Induced by Pten/Akt Signaling in Glioblastoma1

Hu, Xiaoyi, Pandolfi, Pier Paolo, Li, Yi, Koutcher, Jason A, Rosenblum, Marc, Holland, Eric C

Combined activation of Ras and Akt leads to the formation of astrocytic glioblastoma multiforme (GBM) in mice. In human GBMs, AKT is not mutated but is activated in approximately 70% of these tumors,...

c-Myc Enhances Sonic Hedgehog-Induced Medulloblastoma Formation from Nestin-Expressing Neural Progenitors in Mice1

Rao, Ganesh, Pedone, Carolyn A, Coffin, Cheryl M, Holland, Eric C, Fults, Daniel W

Medulloblastomas are malignant brain tumors that arise in the cerebella of children. The presumed cells-of-origin are undifferentiated precursors of granule neurons that occupy the external granule...

MYC Expression Promotes the Proliferation of Neural Progenitor Cells in Culture and In Vivo1

Fults, Dan, Pedone, Carolyn, Dai, Chengkai, Holland, Eric C

Primitive neuroectodermal tumors (PNETs) are pediatric brain tumors that result from defects in signaling molecules governing the growth and differentiation of neural progenitor cells. We used the...

MRI of Mouse Models for Gliomas Shows Similarities to Humans and Can Be Used to Identify Mice for Preclinical Trials

Koutcher, Jason A, Hux, Xiaoyi, Xu, Su, Gade, Terence PF, Leeds, Norman, Zhou, Xiaohong Joe, ...

Magnetic resonance imaging (MRI) has been utilized for screening and detecting brain tumors in mice based upon their imaging characteristics appearance and their pattern of enhancement. Imaging of...

Identification of a multipotent astrocytic stem cell in the immature and adult mouse brain

Laywell, Eric D., Rakic, Pasko, Kukekov, Valery G., Holland, Eric C., Steindler, Dennis A.

The mammalian brain contains a population of neural stem cells (NSC) that can both self-renew and generate progeny along the three lineage pathways of the central nervous system (CNS), but the in...

Basic fibroblast growth factor induces cell migration and proliferation after glia-specific gene transfer in mice

Holland, Eric C., Varmus, Harold E.

Basic fibroblast growth factor (bFGF) is overexpressed in most high-grade human gliomas, implying that it is involved in the pathogenesis of these tumors. To assess the biological effect of...

Dissection of the c-Kit signaling pathway in mouse primordial germ cells by retroviral-mediated gene transfer

De Miguel, Maria P., Cheng, Linzhao, Holland, Eric C., Federspiel, Mark J., Donovan, Peter J.

Establishment of the mammalian germ line is a prerequisite for fertility of the adult animal but we know surprisingly little about the molecular mechanisms regulating germ-line development in...

p190RhoGAP can act to inhibit PDGF-induced gliomas in mice: a putative tumor suppressor encoded on human Chromosome 19q13.3

Wolf, Rebecca M., Draghi, Nicole, Liang, Xiquan, Dai, Chengkai, Uhrbom, Lene, Eklöf, Charlotta, ...

p190RhoGAP and Rho are key regulators of oligodendrocyte differentiation. The gene encoding p190RhoGAP is located at 19q13.3 of the human chromosome, a locus that is deleted in 50%–80% of...

PDGF autocrine stimulation dedifferentiates cultured astrocytes and induces oligodendrogliomas and oligoastrocytomas from neural progenitors and astrocytes in vivo

Dai, Chengkai, Celestino, Joseph C., Okada, Yoshifumi, Louis, David N., Fuller, Greory N., Holland, Eric C.

We present evidence that some low-grade oligodendrogliomas may be comprised of proliferating glial progenitor cells that are blocked in their ability to differentiate, whereas malignant gliomas have...

A constitutively active epidermal growth factor receptor cooperates with disruption of G1 cell-cycle arrest pathways to induce glioma-like lesions in mice

Holland, Eric C., Hively, Wendy P., DePinho, Ronald A., Varmus, Harold E.

The epidermal growth factor receptor (EGFR) gene is amplified or mutated in 30%–50% of human gliobastoma multiforme (GBM). These mutations are associated usually with deletions of the INK4a–ARF...

Modeling mutations in the G1 arrest pathway in human gliomas: overexpression of CDK4 but not loss of INK4a–ARF induces hyperploidy in cultured mouse astrocytes

Holland, Eric C., Hively, Wendy P., Gallo, Vittorio, Varmus, Harold E.

Nearly all human gliomas exhibit alterations in one of three genetic loci governing G1 arrest: INK4a–ARF, CDK4, or RB. To discern the roles of CDK4 amplification and INK4a–ARF loss in...

Differential exoprotease activities confer tumor-specific serum peptidome patterns

Villanueva, Josep, Shaffer, David R., Philip, John, Chaparro, Carlos A., Erdjument-Bromage, Hediye, Olshen, Adam B., ...

Recent studies have established distinctive serum polypeptide patterns through mass spectrometry (MS) that reportedly correlate with clinically relevant outcomes. Wider acceptance of these signatures...

The Characteristics of Astrocytomas and Oligodendrogliomas Are Caused by Two Distinct and Interchangeable Signaling Formats1

Dai, Chengkai, Lyustikman, Yelena, Shih, Alan, Hu, Xiaoyi, Fuller, Gregory N, Rosenblum, Marc, ...

Chronic platelet-derived growth factor (PDGF) signaling in glial progenitors leads to the formation of oligodendrogliomas in mice, whereas chronic combined Ras and Akt signaling leads to...

mTOR Promotes Survival and Astrocytic Characteristics Induced by Pten/Akt Signaling in Glioblastoma1

Hu, Xiaoyi, Pandolfi, Pier Paolo, Li, Yi, Koutcher, Jason A, Rosenblum, Marc, Holland, Eric C

Combined activation of Ras and Akt leads to the formation of astrocytic glioblastoma multiforme (GBM) in mice. In human GBMs, AKT is not mutated but is activated in approximately 70% of these tumors,...

c-Myc Enhances Sonic Hedgehog-Induced Medulloblastoma Formation from Nestin-Expressing Neural Progenitors in Mice1

Rao, Ganesh, Pedone, Carolyn A, Coffin, Cheryl M, Holland, Eric C, Fults, Daniel W

Medulloblastomas are malignant brain tumors that arise in the cerebella of children. The presumed cells-of-origin are undifferentiated precursors of granule neurons that occupy the external granule...

MYC Expression Promotes the Proliferation of Neural Progenitor Cells in Culture and In Vivo1

Fults, Dan, Pedone, Carolyn, Dai, Chengkai, Holland, Eric C

Primitive neuroectodermal tumors (PNETs) are pediatric brain tumors that result from defects in signaling molecules governing the growth and differentiation of neural progenitor cells. We used the...

MRI of Mouse Models for Gliomas Shows Similarities to Humans and Can Be Used to Identify Mice for Preclinical Trials

Koutcher, Jason A, Hux, Xiaoyi, Xu, Su, Gade, Terence PF, Leeds, Norman, Zhou, Xiaohong Joe, ...

Magnetic resonance imaging (MRI) has been utilized for screening and detecting brain tumors in mice based upon their imaging characteristics appearance and their pattern of enhancement. Imaging of...

Notch Signaling Enhances Nestin Expression in Gliomas1*

Shih, Alan H, Holland, Eric C

Recent findings suggest that Notch signaling is active in brain tumors and stem cells and that stem cells or cells with progenitor characteristics contribute to brain tumor formation. These stem...

Astrocytes Give Rise to Oligodendrogliomas and Astrocytomas after Gene Transfer of Polyoma Virus Middle T Antigen in Vivo

Holland, Eric C., Li, Yi, Celestino, Joseph, Dai, Chengkai, Schaefer, Laura, Sawaya, Raymond A., ...

The cells of origin for oligodendrogliomas and astrocytomas are not known but are presumed to be oligodendrocyte and astrocyte precursors, respectively. In this paper we report the generation of...

Insulin-like growth factor binding protein 2 promotes glioma development and progression

Dunlap, Sarah M., Celestino, Joseph, Wang, Hua, Jiang, Rongcai, Holland, Eric C., Fuller, Gregory N., ...

Overexpression of insulin-like growth factor binding protein 2 (IGFBP2) is associated with progression in many types of human cancer. In this study we used a glial-specific transgenic mouse model to...

Constitutive Activation of Raf-1 Induces Glioma Formation in Mice1

Lyustikman, Yelena, Momota, Hiroyuki, Pao, William, Holland, Eric C

In human glioblastoma multiforme (GBM), RAS activity is upregulated in the majority of the tumors. Furthermore, the levels of phospho-mitogen-activated protein kinase/extracellular signal regulated...

PI3K pathway regulates survival of cancer stem cells residing in the perivascular niche following radiation in medulloblastoma in vivo

Hambardzumyan, Dolores, Becher, Oren J., Rosenblum, Marc K., Pandolfi, Pier Paolo, Manova-Todorova, Katia, Holland, Eric C.

Medulloblastomas are brain tumors that arise in the cerebellum of children and contain stem cells in a perivascular niche thought to give rise to recurrence following radiation. We used several mouse...