Ellen S. Vitetta

Failure of vaccination with idiotypic protein or DNA, (+/-IL-2), the depletion of regulatory T cells, or the blockade of CTLA-4 to prolong dormancy in mice with BCL1 lymphoma (2005)

Pop, Laurentiu M., Smallshaw, Joan E., Tucker, Thomas F., Stevenson, Freda K., Vitetta, Ellen S.

Immunization of mice with the idiotype (Id) immunoglobulin from the murine B cell lymphoma, BCL1, before inoculating tumor cells can induce tumor dormancy. In this model, the tumor cells grow for a...

Inhibition of a vaccine-induced anti-tumor B cell response by soluble protein antigen in the absence of continuing T cell help (2005)

Savelyeva, Natalia, King, Catherine A., Vitetta, Ellen S., Stevenson, Freda K.

DNA vaccination can elicit the production of anti-tumor antibodies, thus obviating the need to continuously administer passive antibody. This vaccination strategy is particularly important where...

The Generation and Preclinical Evaulation of Homodimeric Anti-Her-2 Antibodies (1998)

Vitetta, Ellen S.

We have generated a panel of 100 anti-Her-2 MAbs and have characterized them with regard to isotype, epitope recognition and ability to signal apoptosis in Her-2 overexpressing prostate cancer cell...

The Generation and Preclinical Evaluation of Multimeric Anti-Her-2 Antibodies (1998)

Vitetta, Ellen S.

We have generated a panel of 100 anti-Her-2 MAbs and have characterized them with regard to isotype, epitope recognition and ability to signal apoptosis in Her-2-overexpressing breast cancer cell...

The Generation and Preclinical Evaluation of Homodimeric Anti-Her-2 Antibodies (1998)

Vitetta, Ellen S.

Her-2/neu has been selected as a target for immunotherapy based on its prevalence on a variety of tumor cells and the poor prognosis associated with its overexpression. From a panel of a hundred...

The Generation and Preclinical Evaluation of Homodimeric Anti-Her-2 Antibodies (1998)

Vitetta, Ellen S.

Her-2/neu has been selected as a target for immunotherapy based on its prevalence on a variety of tumor cells and the poor prognosis associated with its overexpression. From a panel of a hundred...

Partial characterization of Ia antigens from murine lymphoid cells (1974)

Vitetta, Ellen S., Klein, Jan, Uhr, Jonathan W.

Congenic anti-Ia antisera were used to bind radiolabelled Ia antigens from cells of various strains of mice of known H-2 haplotype. The results indicate that Ia antigens are proteins of molecular...

An anti-CD45RO immunotoxin eliminates T cells latently infected with HIV-1 in vitro

McCoig, Cynthia, Van Dyke, Gregory, Chou, Chin-Sheng, Picker, Louis J., Ramilo, Octavio, Vitetta, Ellen S.

Despite the success of highly active antiretroviral therapy (HAART) in lowering circulating HIV-1 to undetectable levels in most infected individuals, several studies have documented the presence of...

Evidence for a structural motif in toxins and interleukin-2 that may be responsible for binding to endothelial cells and initiating vascular leak syndrome

Baluna, Roxana, Rizo, Josep, Gordon, Brian E., Ghetie, Victor, Vitetta, Ellen S.

The dose-limiting toxicity of interleukin-2 (IL-2) and immunotoxin (IT) therapy in humans is vascular leak syndrome (VLS). VLS has a complex etiology involving damage to vascular endothelial cells...

Homodimerization of tumor-reactive monoclonal antibodies markedly increases their ability to induce growth arrest or apoptosis of tumor cells

Ghetie, Maria-Ana, Podar, Erika M., Ilgen, Amy, Gordon, Brian E., Uhr, Jonathan W., Vitetta, Ellen S.

Monoclonal antibodies (mAbs) that exert antitumor activity can do so by virtue of their effector function and/or their ability to signal growth arrest or cell death. In this study, we demonstrate...

A role for intracellular pH in membrane IgM-mediated cell death of human B lymphomas

Marches, Radu, Vitetta, Ellen S., Uhr, Jonathan W.

We show that anti-IgM-induced cell death in a human B lymphoma cell line, B104, is associated with early intracellular acidification and cell shrinkage. In contrast, another human B cell lymphoma...

Immunoglobulin Synthesis and Secretion by Cells in the Mouse Thymus That Do Not Bear θ Antigen

Vitetta, Ellen S., Uhr, Jonathan W., Boyse, Edward A.

The mouse thymus contains cells that synthesize and secrete minute amounts of immunoglobulin (Ig). These cells were studied by a combination of cytotoxic tests (with alloantisera prepared in congenic...

Zidovudine, Lamivudine, and Abacavir Have Different Effects on Resting Cells Infected with Human Immunodeficiency Virus In Vitro

Saavedra-Lozano, Jesús, McCoig, Cynthia C., Cao, Yanying, Vitetta, Ellen S., Ramilo, Octavio

We have previously described an in vitro model for the evaluation of the effects of different immunomodulatory agents and immunotoxins (ITs) on cells latently infected with human immunodeficiency...

Inhibition of a vaccine-induced anti-tumor B cell response by soluble protein antigen in the absence of continuing T cell help

Savelyeva, Natalia, King, Catherine A., Vitetta, Ellen S., Stevenson, Freda K.

DNA vaccination can elicit the production of anti-tumor antibodies, thus obviating the need to continuously administer passive antibody. This vaccination strategy is particularly important where...

A pilot clinical trial of a recombinant ricin vaccine in normal humans

Vitetta, Ellen S., Smallshaw, Joan E., Coleman, Elaine, Jafri, Hasan, Foster, Callie, Munford, Robert, ...

Ricin, a highly potent toxin produced by castor beans, is classified by the Centers for Disease Control and Prevention as a level B biothreat because it is easily produced, readily available, and...

An anti-CD45RO immunotoxin eliminates T cells latently infected with HIV-1 in vitro

McCoig, Cynthia, Van Dyke, Gregory, Chou, Chin-Sheng, Picker, Louis J., Ramilo, Octavio, Vitetta, Ellen S.

Despite the success of highly active antiretroviral therapy (HAART) in lowering circulating HIV-1 to undetectable levels in most infected individuals, several studies have documented the presence of...

Evidence for a structural motif in toxins and interleukin-2 that may be responsible for binding to endothelial cells and initiating vascular leak syndrome

Baluna, Roxana, Rizo, Josep, Gordon, Brian E., Ghetie, Victor, Vitetta, Ellen S.

The dose-limiting toxicity of interleukin-2 (IL-2) and immunotoxin (IT) therapy in humans is vascular leak syndrome (VLS). VLS has a complex etiology involving damage to vascular endothelial cells...

Homodimerization of tumor-reactive monoclonal antibodies markedly increases their ability to induce growth arrest or apoptosis of tumor cells

Ghetie, Maria-Ana, Podar, Erika M., Ilgen, Amy, Gordon, Brian E., Uhr, Jonathan W., Vitetta, Ellen S.

Monoclonal antibodies (mAbs) that exert antitumor activity can do so by virtue of their effector function and/or their ability to signal growth arrest or cell death. In this study, we demonstrate...

A role for intracellular pH in membrane IgM-mediated cell death of human B lymphomas

Marches, Radu, Vitetta, Ellen S., Uhr, Jonathan W.

We show that anti-IgM-induced cell death in a human B lymphoma cell line, B104, is associated with early intracellular acidification and cell shrinkage. In contrast, another human B cell lymphoma...

Immunoglobulin Synthesis and Secretion by Cells in the Mouse Thymus That Do Not Bear θ Antigen

Vitetta, Ellen S., Uhr, Jonathan W., Boyse, Edward A.

The mouse thymus contains cells that synthesize and secrete minute amounts of immunoglobulin (Ig). These cells were studied by a combination of cytotoxic tests (with alloantisera prepared in congenic...

Zidovudine, Lamivudine, and Abacavir Have Different Effects on Resting Cells Infected with Human Immunodeficiency Virus In Vitro

Saavedra-Lozano, Jesús, McCoig, Cynthia C., Cao, Yanying, Vitetta, Ellen S., Ramilo, Octavio

We have previously described an in vitro model for the evaluation of the effects of different immunomodulatory agents and immunotoxins (ITs) on cells latently infected with human immunodeficiency...

Inhibition of a vaccine-induced anti-tumor B cell response by soluble protein antigen in the absence of continuing T cell help

Savelyeva, Natalia, King, Catherine A., Vitetta, Ellen S., Stevenson, Freda K.

DNA vaccination can elicit the production of anti-tumor antibodies, thus obviating the need to continuously administer passive antibody. This vaccination strategy is particularly important where...

A pilot clinical trial of a recombinant ricin vaccine in normal humans

Vitetta, Ellen S., Smallshaw, Joan E., Coleman, Elaine, Jafri, Hasan, Foster, Callie, Munford, Robert, ...

Ricin, a highly potent toxin produced by castor beans, is classified by the Centers for Disease Control and Prevention as a level B biothreat because it is easily produced, readily available, and...

Selective depletion of alloreactive donor lymphocytes: a novel method to reduce the severity of graft-versus-host disease in older patients undergoing matched sibling donor stem cell transplantation

Solomon, Scott R., Mielke, Stephan, Savani, Bipin N., Montero, Aldemar, Wisch, Laura, Childs, Richard, ...

We have selectively depleted host-reactive donor T cells from peripheral blood stem cell (PBSC) transplant allografts ex vivo using an anti-CD25 immunotoxin. We report a clinical trial to decrease...

CELL SURFACE IMMUNOGLOBULIN : II. ISOLATION AND CHARACTERIZATION OF IMMUNOGLOBULIN FROM MOUSE SPLENIC LYMPHOCYTES

Vitetta, Ellen S., Baur, Sonia, Uhr, Jonathan W.

The proteins on surfaces of living splenic lymphocytes from normal BALB/c mice were iodinated enzymatically. Such cells were fractionated into two sub-populations: one composed almost exclusively of...

CELL SURFACE IMMUNOGLOBULIN : IV. DISTRIBUTION AMONG THYMOCYTES, BONE MARROW CELLS, AND THEIR DERIVED POPULATIONS

Vitetta, Ellen S., Bianco, Celso, Nussenzweig, Victor, Uhr, Jonathan W.

Thymocytes, bone marrow cells, and their derived T and B cell populations were examined for the presence of Ig by the cell surface radioiodination technique. Both IgM and IgG were identified on bone...

CELL SURFACE IMMUNOGLOBULIN : V. RELEASE FROM MURINE SPLENIC LYMPHOCYTES

Vitetta, Ellen S., Uhr, Jonathan W.

Turnover and release of cell surface Ig and secretion of total intracellular Ig has been studied in small lymphocytes from normal mouse spleen. The major findings to emerge are: (a) small lymphocytes...

CELL SURFACE IMMUNOGLOBULIN : VIII. SYNTHESIS, SECRETION, AND CELL SURFACE EXPRESSION OF IMMUNOGLOBULIN IN MURINE THORACIC DUCT LYMPHOCYTES

Uhr, Jonathan W., Vitetta, Ellen S.

CELL SURFACE IMMUNOGLOBULIN : VII. SYNTHESIS, SHEDDING, AND SECRETION OF IMMUNOGLOBULIN BY LYMPHOID CELLS OF GERM-FREE MICE

Vitetta, Ellen S., Grundke-Iqbal, Inge, Holmes, Kathryn V., Uhr, Jonathan W.

Lymphoid cells from the spleen, lymph nodes, and thoracic duct of axenic and control mice were incubated with [3H]tyrosine and synthesis and secretion of protein and Ig studied. It was found that...

CELL SURFACE IMMUNOGLOBULIN : IX. A NEW METHOD FOR THE STUDY OF SYNTHESIS, INTRACELLULAR TRANSPORT, AND EXTERIORIZATION IN MURINE SPLENOCYTES

Vitetta, Ellen S., Uhr, Jonathan W.

A new method for the detection of cell surface immunoglobulin labeled with isotopic precursors is described. The method consists of the aggregation of surface Ig on cells with specific antibody...

CELL SURFACE IMMUNOGLOBULIN : X. Identification of an IgD-Like Molecule on the Surface of Murine Splenocytes

Melcher, Ulrich, Vitetta, Ellen S., McWilliams, Michael, Lamm, Michael E., Phillips-Quagliata, Julia M., Uhr, Jonathan W.

SYNTHESIS, INTRACELLULAR DISTRIBUTION, AND SECRETION OF IMMUNOGLOBULIN AND H-2 ANTIGEN IN MURINE SPLENOCYTES

Wernet, Dorothee, Vitetta, Ellen S., Uhr, Jonathan W., Boyse, Edward A.

A/J spleen cells were labeled with [3H]leucine and at intervals thereafter were homogenized and separated into microsomes and cell sap. Ig and H-2 antigens were assayed in the cell fractions and cell...

Reconstitution of FOXP3+ regulatory T cells (Tregs) after CD25-depleted allotransplantion in elderly patients and association with acute graft-versus-host disease

Mielke, Stephan, Rezvani, Katayoun, Savani, Bipin N., Nunes, Raquel, Yong, Agnes S. M., Schindler, John, ...

Selective depletion (SD) of host-reactive donor T cells from allogeneic stem-cell transplants (SCTs) using an anti-CD25 immunotoxin (IT) is a strategy to prevent acute graft-versus-host disease...