Kufel, Joanna, Allmang, Christine, Chanfreau, Guillaume, Petfalski, Elisabeth, Lafontaine, Denis L. J., Tollervey, David
Almost all small eukaryotic RNAs are processed from transiently stabilized 3′-extended forms. A key question is how and why such intermediates are stabilized and how they can then be processed to...
Degradation of ribosomal RNA precursors by the exosome
Allmang, Christine, Mitchell, Philip, Petfalski, Elisabeth, Tollervey, David
The yeast exosome is a complex of 3′→5′ exonucleases involved in RNA processing and degradation. All 11 known components of the exosome are required during 3′ end processing of the 5.8S rRNA....
Processing of the Precursors to Small Nucleolar RNAs and rRNAs Requires Common Components
Petfalski, Elisabeth, Dandekar, Thomas, Henry, Yves, Tollervey, David
The genes encoding the small nucleolar RNA (snoRNA) species snR190 and U14 are located close together in the genome of Saccharomyces cerevisiae. Here we report that these two snoRNAs are synthesized...
Gadal, Olivier, Strauß, Daniela, Braspenning, Joris, Hoepfner, Dominic, Petfalski, Elisabeth, Philippsen, Peter, ...
Ribosomal precursor particles are assembled in the nucleolus before export into the cytoplasm. Using a visual assay for nuclear accumulation of 60S subunits, we have isolated several...
60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm
Nissan, Tracy A., Baßler, Jochen, Petfalski, Elisabeth, Tollervey, David, Hurt, Ed
60S ribosomes undergo initial assembly in the nucleolus before export to the cytoplasm and recent analyses have identified several nucleolar pre-60S particles. To unravel the steps in the pathway of...
The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes
Schäfer, Thorsten, Strauß, Daniela, Petfalski, Elisabeth, Tollervey, David, Hurt, Ed
Recent reports have increased our knowledge of the consecutive steps during 60S ribosome biogenesis substantially, but 40S subunit formation is less well understood. Here, we investigate the...
Rrp47p Is an Exosome-Associated Protein Required for the 3′ Processing of Stable RNAs
Mitchell, Philip, Petfalski, Elisabeth, Houalla, Rym, Podtelejnikov, Alexandre, Mann, Matthias, Tollervey, David
Related exosome complexes of 3′→5′ exonucleases are present in the nucleus and the cytoplasm. Purification of exosome complexes from whole-cell lysates identified a Mg2+-labile factor present...
Newton, Kathryn, Petfalski, Elisabeth, Tollervey, David, Cáceres, Javier F.
Fibrillarin, a protein component of C/D box small nucleolar ribonucleoproteins (snoRNPs), directs 2′-O-methylation of rRNA and is also involved in other aspects of rRNA processing. A gene trap...
The yeast exosome and human PM–Scl are related complexes of 3′ → 5′ exonucleases
Allmang, Christine, Petfalski, Elisabeth, Podtelejnikov, Alexandre, Mann, Matthias, Tollervey, David, Mitchell, Philip
We previously identified a complex of 3′ → 5′ exoribonucleases, designated the exosome, that is expected to play a major role in diverse RNA processing and degradation pathways. Further...
Functional link between ribosome formation and biogenesis of iron–sulfur proteins
Yarunin, Alexander, Panse, Vikram Govind, Petfalski, Elisabeth, Dez, Christophe, Tollervey, David, Hurt, Ed
In genetic screens for ribosomal export mutants, we identified CFD1, NBP35 and NAR1 as factors involved in ribosome biogenesis. Notably, these components were recently reported to function in...
Formation and nuclear export of tRNA, rRNA and mRNA is regulated by the ubiquitin ligase Rsp5p
Neumann, Silvia, Petfalski, Elisabeth, Brügger, Britta, Großhans, Helge, Wieland, Felix, Tollervey, David, ...
The yeast ubiquitin-protein ligase Rsp5p regulates processes as diverse as polII transcription and endocytosis. Here, we identify Rsp5p in a screen for tRNA export (tex) mutants. The tex23-1/rsp5-3...
Kufel, Joanna, Allmang, Christine, Chanfreau, Guillaume, Petfalski, Elisabeth, Lafontaine, Denis L. J., Tollervey, David
Almost all small eukaryotic RNAs are processed from transiently stabilized 3′-extended forms. A key question is how and why such intermediates are stabilized and how they can then be processed to...
Degradation of ribosomal RNA precursors by the exosome
Allmang, Christine, Mitchell, Philip, Petfalski, Elisabeth, Tollervey, David
The yeast exosome is a complex of 3′→5′ exonucleases involved in RNA processing and degradation. All 11 known components of the exosome are required during 3′ end processing of the 5.8S rRNA....
Processing of the Precursors to Small Nucleolar RNAs and rRNAs Requires Common Components
Petfalski, Elisabeth, Dandekar, Thomas, Henry, Yves, Tollervey, David
The genes encoding the small nucleolar RNA (snoRNA) species snR190 and U14 are located close together in the genome of Saccharomyces cerevisiae. Here we report that these two snoRNAs are synthesized...
Gadal, Olivier, Strauß, Daniela, Braspenning, Joris, Hoepfner, Dominic, Petfalski, Elisabeth, Philippsen, Peter, ...
Ribosomal precursor particles are assembled in the nucleolus before export into the cytoplasm. Using a visual assay for nuclear accumulation of 60S subunits, we have isolated several...
60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm
Nissan, Tracy A., Baßler, Jochen, Petfalski, Elisabeth, Tollervey, David, Hurt, Ed
60S ribosomes undergo initial assembly in the nucleolus before export to the cytoplasm and recent analyses have identified several nucleolar pre-60S particles. To unravel the steps in the pathway of...
The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes
Schäfer, Thorsten, Strauß, Daniela, Petfalski, Elisabeth, Tollervey, David, Hurt, Ed
Recent reports have increased our knowledge of the consecutive steps during 60S ribosome biogenesis substantially, but 40S subunit formation is less well understood. Here, we investigate the...
Rrp47p Is an Exosome-Associated Protein Required for the 3′ Processing of Stable RNAs
Mitchell, Philip, Petfalski, Elisabeth, Houalla, Rym, Podtelejnikov, Alexandre, Mann, Matthias, Tollervey, David
Related exosome complexes of 3′→5′ exonucleases are present in the nucleus and the cytoplasm. Purification of exosome complexes from whole-cell lysates identified a Mg2+-labile factor present...
Newton, Kathryn, Petfalski, Elisabeth, Tollervey, David, Cáceres, Javier F.
Fibrillarin, a protein component of C/D box small nucleolar ribonucleoproteins (snoRNPs), directs 2′-O-methylation of rRNA and is also involved in other aspects of rRNA processing. A gene trap...
The yeast exosome and human PM–Scl are related complexes of 3′ → 5′ exonucleases
Allmang, Christine, Petfalski, Elisabeth, Podtelejnikov, Alexandre, Mann, Matthias, Tollervey, David, Mitchell, Philip
We previously identified a complex of 3′ → 5′ exoribonucleases, designated the exosome, that is expected to play a major role in diverse RNA processing and degradation pathways. Further...
Functional link between ribosome formation and biogenesis of iron–sulfur proteins
Yarunin, Alexander, Panse, Vikram Govind, Petfalski, Elisabeth, Dez, Christophe, Tollervey, David, Hurt, Ed
In genetic screens for ribosomal export mutants, we identified CFD1, NBP35 and NAR1 as factors involved in ribosome biogenesis. Notably, these components were recently reported to function in...
Formation and nuclear export of tRNA, rRNA and mRNA is regulated by the ubiquitin ligase Rsp5p
Neumann, Silvia, Petfalski, Elisabeth, Brügger, Britta, Großhans, Helge, Wieland, Felix, Tollervey, David, ...
The yeast ubiquitin-protein ligase Rsp5p regulates processes as diverse as polII transcription and endocytosis. Here, we identify Rsp5p in a screen for tRNA export (tex) mutants. The tex23-1/rsp5-3...
Gadal, Olivier, Strauss, Daniela, Petfalski, Elisabeth, Gleizes, Pierre-Emmanuel, Gas, Nicole, Tollervey, David, ...
Many analyses have examined subnucleolar structures in eukaryotic cells, but the relationship between morphological structures, pre-rRNA processing, and ribosomal particle assembly has remained...