Elaine Fuchs-Stem Cells and Their Lineages in Skin (2007)
Includes descriptive metadata provided by producer in MP4 file: "Discovery Lecture Series - Videos - Elaine Fuchs-Stem Cells and Their Lineages in Skin."
Elaine Fuchs-Stem Cells and Their Lineages in Skin (2007)
Includes descriptive metadata provided by producer in MP4 file: "Discovery Lecture Series - Videos - Elaine Fuchs-Stem Cells and Their Lineages in Skin."
Molecular Dissection of Mesenchymal–Epithelial Interactions in the Hair Follicle (2005)
Michael Rendl, Lisa Lewis, Elaine Fuchs
Determining the molecular signature of the cells that orchestrate hair follicle growth generates new insights that will aid in understanding the normal biology and disease states of this tissue.
Molecular Dissection of Mesenchymal–Epithelial Interactions in the Hair Follicle (2005)
Michael Rendl, Lisa Lewis, Elaine Fuchs
De novo hair follicle formation in embryonic skin and new hair growth in adult skin are initiated when specialized mesenchymal dermal papilla (DP) cells send cues to multipotent epithelial stem...
A Signaling Pathway Involving TGF-β2 and Snail in Hair Follicle Morphogenesis (2005)
Colin Jamora, Pedro Lee, Pawel Kocieniewski, Mohamad Azhar, Ryoichi Hosokawa, Yang Chai, ...
The study of hair follicle morphogenesis provides insights into how cells within a sheet can be triggered to proliferate, remodel, and form buds - a recurring theme in development.
A Signaling Pathway Involving TGF-β2 and Snail in Hair Follicle Morphogenesis (2005)
Colin Jamora, Pedro Lee, Pawel Kocieniewski, Mohamad Azhar, Ryoichi Hosokawa, Yang Chai, ...
In a common theme of organogenesis, certain cells within a multipotent epithelial sheet exchange signals with their neighbors and develop into a bud structure. Using hair bud morphogenesis as a...
Targeted Disruption of the Murine zyxin Gene. (2003)
Hoffman, Laura M., Nix, David A., Benson, Beverly, Boot-Hanford, Ray, Gustafsson, Erika, Jamora, Colin, ...
Abstract is not available
In vitro properties of hnRNP A2: a role in telomere protection and telomerase recruitment. (2001)
Moran, Kim, Kennedy, Derek, Smith, Ross, Elaine Fuchs, Joan Brugge
No
Perez-Morano, Mirna, Fuchs, Elaine
Cadherins the primary regulators of the overall state of epithelial cell contact and facilitate an appropriate cytoskeletal organization and the establishment of many other kinds of cell interactions...
Perez-Moreno, Mirna, Fuchs, Elaine
At present, it remains obscure whether E-cadherin directly transfers growth inhibitory signals to the cells, or if other types of molecular cell interactions indirectly influenced by the...
Vasioukhin, Valeri, Degenstein, Linda, Wise, Bart, Fuchs, Elaine
Gene knockout technology has provided a powerful tool for functional analyses of genes expressed preferentially in a particular tissue. Given marked similarities between human and mouse skin, such...
Wang, Xiaoming, Zinkel, Sandra, Polonsky, Kenneth, Fuchs, Elaine
Keratinocytes are potentially appealing vehicles for the delivery of secreted gene products because they can be transferred to human skin by the relatively simple procedure of grafting. Adult human...
A dysfunctional desmin mutation in a patient with severe generalized myopathy
Muñoz-Mármol, Ana M., Strasser, Geraldine, Isamat, Marcos, Coulombe, Pierre A., Yang, Yanmin, Roca, Xavier, ...
Mice lacking desmin produce muscle fibers with Z disks and normal sarcomeric organization. However, the muscles are mechanically fragile and degenerate upon repeated contractions. We report here a...
Identification and dissection of an enhancer controlling epithelial gene expression in skin
Sinha, Satrajit, Fuchs, Elaine
Keratins 14 and 5 are the structural hallmarks of the basal keratinocytes of the epidermis and outer root sheath (ORS) of the hair follicle. Their genes are controlled in a tissue-specific manner and...
Defining the Regulatory Factors Required for Epidermal Gene Expression
Sinha, Satrajit, Degenstein, Linda, Copenhaver, Cedith, Fuchs, Elaine
Keratins K5 and K14 are the hallmarks of mitotically active keratinocytes of stratified epithelia. They are transcribed at a high level and in a tissue-specific manner, enabling us to use the K14...
Li, Baoan, Mackay, Douglas R., Dai, Qian, Li, Tony W. H., Nair, Mahalakshmi, Fallahi, Magid, ...
Drosophila ovo/svb (dovo) is required for epidermal cuticle/denticle differentiation and is genetically downstream of the wg signaling pathway. Similarly, a mouse homolog of dovo, movo1, is required...
Kaufman, Charles K., Sinha, Satrajit, Bolotin, Diana, Fan, Jie, Fuchs, Elaine
In this report, we explored the mechanisms underlying keratinocyte-specific and differentiation-specific gene expression in the skin. We have identified five keratinocyte-specific, open chromatin...
Protection of the intestinal mucosa by intraepithelial γδ T cells
Chen, Yaping, Chou, Kevin, Fuchs, Elaine, Havran, Wendy L., Boismenu, Richard
γδ intraepithelial T lymphocytes (IEL) represent a major T cell population within the intestine of unclear functional relevance. The role of intestinal γδ IEL was evaluated in the dextran sodium...
Targeted Disruption of the Murine zyxin Gene
Hoffman, Laura M., Nix, David A., Benson, Beverly, Boot-Hanford, Ray, Gustafsson, Erika, Jamora, Colin, ...
Zyxin is an evolutionarily conserved protein that is concentrated at sites of cell adhesion, where it associates with members of the Enabled (Ena)/vasodilator-stimulated phosphoprotein (VASP) family...
GATA-3: an unexpected regulator of cell lineage determination in skin
Kaufman, Charles K., Zhou, Ping, Amalia Pasolli, H., Rendl, Michael, Bolotin, Diana, Lim, Kim-Chew, ...
Multipotent skin stem cells give rise to epidermis and its appendages, including the hair follicle. The Lef-1/Tcf family of Wnt-regulated transcription factors plays a major role in specification of...
Differential regulation of midbrain dopaminergic neuron development by Wnt-1, Wnt-3a, and Wnt-5a
Castelo-Branco, Gonçalo, Wagner, Joseph, Rodriguez, Francisco J., Kele, Julianna, Sousa, Kyle, Rawal, Nina, ...
The Wnts are a family of glycoproteins that regulate cell proliferation, fate decisions, and differentiation. In our study, we examined the contribution of Wnts to the development of ventral midbrain...
Stem cells of the skin epithelium
Tissue stem cells form the cellular base for organ homeostasis and repair. Stem cells have the unusual ability to renew themselves over the lifetime of the organ while producing daughter cells that...
Dai, Xing, Schonbaum, Christopher, Degenstein, Linda, Bai, Wenyu, Mahowald, Anthony, Fuchs, Elaine
The Drosophila svb/ovo gene gives rise to differentially expressed transcripts encoding a zinc finger protein. svb/ovo has two distinct genetic functions: shavenbaby (svb) is required for proper...
Tinkle, Christopher L., Lechler, Terry, Pasolli, H. Amalia, Fuchs, Elaine
Loss of E-cadherin has been associated with human cancers, and yet in the early mouse embryo and the lactating mammary gland, the E-cadherin null state results in tissue dysfunction and cell death....
A Signaling Pathway Involving TGF-β2 and Snail in Hair Follicle Morphogenesis
Jamora, Colin, Lee, Pedro, Kocieniewski, Pawel, Azhar, Mohamad, Hosokawa, Ryoichi, Chai, Yang, ...
In a common theme of organogenesis, certain cells within a multipotent epithelial sheet exchange signals with their neighbors and develop into a bud structure. Using hair bud morphogenesis as a...
Defining the impact of β-catenin/Tcf transactivation on epithelial stem cells
Lowry, William E., Blanpain, Cedric, Nowak, Jonathan A., Guasch, Geraldine, Lewis, Lisa, Fuchs, Elaine
Wnt signaling has been implicated in stem cell (SC) biology, but little is known about how stabilized β-catenin functions within native SC niches. We address this by defining the impact of...
Molecular Dissection of Mesenchymal–Epithelial Interactions in the Hair Follicle
Rendl, Michael, Lewis, Lisa, Fuchs, Elaine
De novo hair follicle formation in embryonic skin and new hair growth in adult skin are initiated when specialized mesenchymal dermal papilla (DP) cells send cues to multipotent epithelial stem...
Sgk3 links growth factor signaling to maintenance of progenitor cells in the hair follicle
Alonso, Laura, Okada, Hitoshi, Pasolli, Hilda Amalia, Wakeham, Andrew, You-Ten, Annick Itie, Mak, Tak W., ...
Tyrosine kinase growth factor receptor signaling influences proliferation, survival, and apoptosis. Hair follicles undergo cycles of proliferation and apoptotic regression, offering an excellent...
Asymmetric cell divisions promote stratification and differentiation of mammalian skin
The epidermis is a stratified squamous epithelium forming the barrier that excludes harmful microbes and retains body fluids. To perform these functions, proliferative basal cells in the innermost...
Tcf3 and Lef1 regulate lineage differentiation of multipotent stem cells in skin
Merrill, Bradley J., Gat, Uri, DasGupta, Ramanuj, Fuchs, Elaine
In skin, multipotent stem cells generate the keratinocytes of the epidermis, sebaceous gland, and hair follicles. In this paper, we show that Tcf3 and Lef1 control these differentiation lineages. In...
Links between α-catenin, NF-κB, and squamous cell carcinoma in skin
Kobielak, Agnieszka, Fuchs, Elaine
Cancers display a diverse set of cellular defects, which are thought to be elicited by multiple genetic mutations. In this study, we show that when a single adherens junction protein, α-catenin, is...
Haegebarth, Andrea, Bie, Wenjun, Yang, Ruyan, Crawford, Susan E., Vasioukhin, Valeri, Fuchs, Elaine, ...
Protein tyrosine kinase 6 (PTK6) (also called Brk or Sik) is an intracellular tyrosine kinase that is expressed in breast cancer and normal epithelial linings. In adult mice, PTK6 expression is high...
Vasioukhin, Valeri, Degenstein, Linda, Wise, Bart, Fuchs, Elaine
Gene knockout technology has provided a powerful tool for functional analyses of genes expressed preferentially in a particular tissue. Given marked similarities between human and mouse skin, such...
Wang, Xiaoming, Zinkel, Sandra, Polonsky, Kenneth, Fuchs, Elaine
Keratinocytes are potentially appealing vehicles for the delivery of secreted gene products because they can be transferred to human skin by the relatively simple procedure of grafting. Adult human...
A dysfunctional desmin mutation in a patient with severe generalized myopathy
Muñoz-Mármol, Ana M., Strasser, Geraldine, Isamat, Marcos, Coulombe, Pierre A., Yang, Yanmin, Roca, Xavier, ...
Mice lacking desmin produce muscle fibers with Z disks and normal sarcomeric organization. However, the muscles are mechanically fragile and degenerate upon repeated contractions. We report here a...
Identification and dissection of an enhancer controlling epithelial gene expression in skin
Sinha, Satrajit, Fuchs, Elaine
Keratins 14 and 5 are the structural hallmarks of the basal keratinocytes of the epidermis and outer root sheath (ORS) of the hair follicle. Their genes are controlled in a tissue-specific manner and...
Defining the Regulatory Factors Required for Epidermal Gene Expression
Sinha, Satrajit, Degenstein, Linda, Copenhaver, Cedith, Fuchs, Elaine
Keratins K5 and K14 are the hallmarks of mitotically active keratinocytes of stratified epithelia. They are transcribed at a high level and in a tissue-specific manner, enabling us to use the K14...
Li, Baoan, Mackay, Douglas R., Dai, Qian, Li, Tony W. H., Nair, Mahalakshmi, Fallahi, Magid, ...
Drosophila ovo/svb (dovo) is required for epidermal cuticle/denticle differentiation and is genetically downstream of the wg signaling pathway. Similarly, a mouse homolog of dovo, movo1, is required...
Kaufman, Charles K., Sinha, Satrajit, Bolotin, Diana, Fan, Jie, Fuchs, Elaine
In this report, we explored the mechanisms underlying keratinocyte-specific and differentiation-specific gene expression in the skin. We have identified five keratinocyte-specific, open chromatin...
Protection of the intestinal mucosa by intraepithelial γδ T cells
Chen, Yaping, Chou, Kevin, Fuchs, Elaine, Havran, Wendy L., Boismenu, Richard
γδ intraepithelial T lymphocytes (IEL) represent a major T cell population within the intestine of unclear functional relevance. The role of intestinal γδ IEL was evaluated in the dextran sodium...
Targeted Disruption of the Murine zyxin Gene
Hoffman, Laura M., Nix, David A., Benson, Beverly, Boot-Hanford, Ray, Gustafsson, Erika, Jamora, Colin, ...
Zyxin is an evolutionarily conserved protein that is concentrated at sites of cell adhesion, where it associates with members of the Enabled (Ena)/vasodilator-stimulated phosphoprotein (VASP) family...
GATA-3: an unexpected regulator of cell lineage determination in skin
Kaufman, Charles K., Zhou, Ping, Amalia Pasolli, H., Rendl, Michael, Bolotin, Diana, Lim, Kim-Chew, ...
Multipotent skin stem cells give rise to epidermis and its appendages, including the hair follicle. The Lef-1/Tcf family of Wnt-regulated transcription factors plays a major role in specification of...
Differential regulation of midbrain dopaminergic neuron development by Wnt-1, Wnt-3a, and Wnt-5a
Castelo-Branco, Gonçalo, Wagner, Joseph, Rodriguez, Francisco J., Kele, Julianna, Sousa, Kyle, Rawal, Nina, ...
The Wnts are a family of glycoproteins that regulate cell proliferation, fate decisions, and differentiation. In our study, we examined the contribution of Wnts to the development of ventral midbrain...
Stem cells of the skin epithelium
Tissue stem cells form the cellular base for organ homeostasis and repair. Stem cells have the unusual ability to renew themselves over the lifetime of the organ while producing daughter cells that...
Tcf3 and Lef1 regulate lineage differentiation of multipotent stem cells in skin
Merrill, Bradley J., Gat, Uri, DasGupta, Ramanuj, Fuchs, Elaine
In skin, multipotent stem cells generate the keratinocytes of the epidermis, sebaceous gland, and hair follicles. In this paper, we show that Tcf3 and Lef1 control these differentiation lineages. In...
Dai, Xing, Schonbaum, Christopher, Degenstein, Linda, Bai, Wenyu, Mahowald, Anthony, Fuchs, Elaine
The Drosophila svb/ovo gene gives rise to differentially expressed transcripts encoding a zinc finger protein. svb/ovo has two distinct genetic functions: shavenbaby (svb) is required for proper...
Tinkle, Christopher L., Lechler, Terry, Pasolli, H. Amalia, Fuchs, Elaine
Loss of E-cadherin has been associated with human cancers, and yet in the early mouse embryo and the lactating mammary gland, the E-cadherin null state results in tissue dysfunction and cell death....
A Signaling Pathway Involving TGF-β2 and Snail in Hair Follicle Morphogenesis
Jamora, Colin, Lee, Pedro, Kocieniewski, Pawel, Azhar, Mohamad, Hosokawa, Ryoichi, Chai, Yang, ...
In a common theme of organogenesis, certain cells within a multipotent epithelial sheet exchange signals with their neighbors and develop into a bud structure. Using hair bud morphogenesis as a...
Defining the impact of β-catenin/Tcf transactivation on epithelial stem cells
Lowry, William E., Blanpain, Cedric, Nowak, Jonathan A., Guasch, Geraldine, Lewis, Lisa, Fuchs, Elaine
Wnt signaling has been implicated in stem cell (SC) biology, but little is known about how stabilized β-catenin functions within native SC niches. We address this by defining the impact of...
Siegel, Dawn H., Ashton, Gabrielle H. S., Penagos, Homero G., Lee, James V., Feiler, Heidi S., Wilhelmsen, Kirk C., ...
Kindler syndrome is an autosomal recessive disorder characterized by neonatal blistering, sun sensitivity, atrophy, abnormal pigmentation, and fragility of the skin. Linkage and homozygosity analysis...
Molecular Dissection of Mesenchymal–Epithelial Interactions in the Hair Follicle
Rendl, Michael, Lewis, Lisa, Fuchs, Elaine
De novo hair follicle formation in embryonic skin and new hair growth in adult skin are initiated when specialized mesenchymal dermal papilla (DP) cells send cues to multipotent epithelial stem...
Links between α-catenin, NF-κB, and squamous cell carcinoma in skin
Kobielak, Agnieszka, Fuchs, Elaine
Cancers display a diverse set of cellular defects, which are thought to be elicited by multiple genetic mutations. In this study, we show that when a single adherens junction protein, α-catenin, is...
Haegebarth, Andrea, Bie, Wenjun, Yang, Ruyan, Crawford, Susan E., Vasioukhin, Valeri, Fuchs, Elaine, ...
Protein tyrosine kinase 6 (PTK6) (also called Brk or Sik) is an intracellular tyrosine kinase that is expressed in breast cancer and normal epithelial linings. In adult mice, PTK6 expression is high...
Basal Cells Are a Multipotent Progenitor Capable of Renewing the Bronchial Epithelium
Hong, Kyung U., Reynolds, Susan D., Watkins, Simon, Fuchs, Elaine, Stripp, Barry R.
Commitment of the pulmonary epithelium to bronchial and bronchiolar airway lineages occurs during the transition from pseudoglandular to cannalicular phases of lung development, suggesting that...
Canonical notch signaling functions as a commitment switch in the epidermal lineage
Blanpain, Cédric, Lowry, William E., Pasolli, H. Amalia, Fuchs, Elaine
Mammalian epidermis consists of a basal layer of proliferative progenitors that gives rise to multiple differentiating layers to provide a waterproof envelope covering the skin surface. To accomplish...
Li, Jinsong, Greco, Valentina, Guasch, Géraldine, Fuchs, Elaine, Mombaerts, Peter
Adult stem cells represent unique populations of undifferentiated cells with self-renewal capacity. In many tissues, stem cells divide less often than their progeny. It has been widely speculated,...
AP-2α: a regulator of EGF receptor signaling and proliferation in skin epidermis
Wang, Xuan, Bolotin, Diana, Chu, David H., Polak, Lisa, Williams, Trevor, Fuchs, Elaine
AP-2 transcription factors have been implicated in epidermal biology, but their functional significance has remained elusive. Using conditional knockout technology, we show that AP-2α is essential...
Desmoplakin: an unexpected regulator of microtubule organization in the epidermis
Despite their importance in cell shape and polarity generation, the organization of microtubules in differentiated cells and tissues remains relatively unexplored in mammals. We generated transgenic...
Focal adhesion kinase modulates tension signaling to control actin and focal adhesion dynamics
Schober, Markus, Raghavan, Srikala, Nikolova, Maria, Polak, Lisa, Pasolli, H. Amalia, Beggs, Hilary E., ...
In response to αβ1 integrin signaling, transducers such as focal adhesion kinase (FAK) become activated, relaying to specific machineries and triggering distinct cellular responses. By...
Kobielak, Krzysztof, Stokes, Nicole, De La Cruz, June, Polak, Lisa, Fuchs, Elaine
During the hair cycle, follicle stem cells (SCs) residing in a specialized niche called the “bulge” undergo bouts of quiescence and activation to cyclically regenerate new hairs. Developmental...
Sgk3 links growth factor signaling to maintenance of progenitor cells in the hair follicle
Alonso, Laura, Okada, Hitoshi, Pasolli, Hilda Amalia, Wakeham, Andrew, You-Ten, Annick Itie, Mak, Tak W., ...
Tyrosine kinase growth factor receptor signaling influences proliferation, survival, and apoptosis. Hair follicles undergo cycles of proliferation and apoptotic regression, offering an excellent...
Coordinating cytoskeletal tracks to polarize cellular movements
Kodama, Atsuko, Lechler, Terry, Fuchs, Elaine
For many years after the discovery of actin filaments and microtubules, it was widely assumed that their polymerization, organization, and functions were largely distinct. However, in recent years it...
Progressive Kidney Degeneration in Mice Lacking Tensin
Lo, Su Hao, Yu, Qian-Chun, Degenstein, Linda, Chen, Lan Bo, Fuchs, Elaine
Tensin is a focal adhesion phosphoprotein that binds to F-actin and contains a functional Src homology 2 domain. To explore the biological functions of tensin, we cloned the mouse tensin gene,...
Functional Differences between Keratins of Stratified and Simple Epithelia
Hutton, Elizabeth, Paladini, Rudolph D., Yu, Qian-Chun, Yen, Mei, Coulombe, Pierre A., Fuchs, Elaine
Dividing populations of stratified and simple epithelial tissues express keratins 5 and 14, and keratins 8 and 18, respectively. It has been suggested that these keratins form a mechanical framework...
Defining the Interactions Between Intermediate Filaments and Desmosomes
Smith, Elizabeth A., Fuchs, Elaine
Desmoplakin (DP), plakoglobin (PG), and plakophilin 1 (PP1) are desmosomal components lacking a transmembrane domain, thus making them candidate linker proteins for connecting intermediate filaments...
Yang, Zhao-hui, Gallicano, G. Ian, Yu, Qian-Chun, Fuchs, Elaine
Basonuclin is a zinc finger protein that was thought to be restricted to keratinocytes of stratified squamous epithelia. In epidermis, basonuclin is associated with the nuclei of mitotically active...
DasGupta, Ramanuj, Rhee, Horace, Fuchs, Elaine
Wnt signaling orchestrates morphogenetic processes in which changes in gene expression are associated with dramatic changes in cell organization within developing tissue/organss. Upon signaling,...