Baldin, Véronique, Militello, Muriel, Thomas, Yann, Doucet, Christine, Fic, Weronika, Boireau, Stephanie, ...
In eukaryotic cells, proteasomes play an essential role in intracellular proteolysis and are involved in the control of most biological processes through regulated degradation of key proteins....
Baldin, Véronique, Militello, Muriel, Thomas, Yann, Doucet, Christine, Fic, Weronika, Boireau, Stephanie, ...
In eukaryotic cells, proteasomes play an essential role in intracellular proteolysis and are involved in the control of most biological processes through regulated degradation of key proteins....
Durand, Sébastien, Cougot, Nicolas, Mahuteau-Betzer, Florence, Nguyen, Chi-Hung, Grierson, David, Bertrand, Edouard, ...
In mammals, nonsense-mediated mRNA decay (NMD) is a quality control mechanism that degrades mRNAs harboring a premature termination codon, in order to prevent the synthesis of truncated proteins. To...
Durand, Sébastien, Cougot, Nicolas, Mahuteau-Betzer, Florence, Nguyen, Chi-Hung, Grierson, David, Bertrand, Edouard, ...
In mammals, nonsense-mediated mRNA decay (NMD) is a quality control mechanism that degrades mRNAs harboring a premature termination codon, in order to prevent the synthesis of truncated proteins. To...
A real-time view of the TAR:Tat:P-TEFb complex at HIV-1 transcription sites (2007)
Molle, Dorothée, Maiuri, Paolo, Boireau, Stéphanie, Bertrand, Edouard, Knezevich, Anna, Marcello, Alessandro, ...
Abstract HIV-1 transcription is tightly regulated: silent in long-term latency and highly active in acutely-infected cells. Transcription is activated by the viral protein Tat, which recruits the...
Valentin, Guillaume, Verheggen, Céline, Piolot, Tristan, Neel, Henry, Coppey-Moisan, Maïté, Bertrand, Edouard
Darzacq, Xavier, Tucker, Karen E., Matera, A.Gregory, Bertrand, Edouard, Kiss, Tam S
Biogenesis of functional spliceosomal small nuclear RNAs (snRNAs) includes the post-transcriptional covalent modification of numerous internal nucleotides. We have recently demonstrated that...
Mouaikel, John, Narayanan, Usha, Verheggen, C Line, Matera, A. Gregory, Bertrand, Edouard, Tazi, Jamal, ...
The biogenesis of spliceosomal small nuclear ribonucleoproteins (snRNPs) requires the cytoplasmic assembly of the Sm-core complex, followed by the hypermethylation of the small nuclear RNA (snRNA) 5'...
Samarsky, Dmitry A., Ferbeyre, Gerardo, Bertrand, Edouard, Singer, Robert H., Cedergren, Robert, Fournier, Maurille J.
A hammerhead ribozyme has been localized to the yeast nucleolus by using the U3 small nucleolar RNA as a carrier. The hybrid small nucleolar RNA:ribozyme, designated a “snorbozyme,” is...
Kendall, Ann, Hull, Melissa W., Bertrand, Edouard, Good, Paul D., Singer, Robert H., Engelke, David R.
In the budding yeast, Saccharomyces cerevisiae, actively transcribed tRNA genes can negatively regulate adjacent RNA polymerase II (pol II)-transcribed promoters. This tRNA gene-mediated silencing is...
Cell Cycle-dependent Recruitment of Telomerase RNA and Cajal Bodies to Human Telomeres
Jády, Beáta E., Richard, Patricia, Bertrand, Edouard, Kiss, Tamás
Telomerase is a ribonucleoprotein enzyme that counteracts replicative telomere erosion by adding telomeric sequence repeats onto chromosome ends. Despite its well-established role in telomere...
King, Thomas H., Decatur, Wayne A., Bertrand, Edouard, Maxwell, E. Stuart, Fournier, Maurille J.
Biogenesis of small nucleolar RNA-protein complexes (snoRNPs) consists of synthesis of the snoRNA and protein components, snoRNP assembly, and localization to the nucleolus. Recently, two...
Alkaline fixation drastically improves the signal of in situ hybridization
Basyuk, Eugenia, Bertrand, Edouard, Journot, Laurent
In situ hybridization (ISH) is widely used to detect DNA and RNA sequences within the cell and tissue sections. The important step in performing this technique is tissue fixation. We investigated the...
Monitoring Retroviral RNA Dimerization In Vivo via Hammerhead Ribozyme Cleavage
Pal, Bijay K., Scherer, Lisa, Zelby, Laurie, Bertrand, Edouard, Rossi, John J.
We have used a strategy for colocalization of Psi (Ψ)-tethered ribozymes and targets to demonstrate that Ψ sequences are capable of specific interaction in the cytoplasm of both packaging and...
Verheggen, Céline, Mouaikel, John, Thiry, Marc, Blanchard, Jean-Marie, Tollervey, David, Bordonné, Remi, ...
Nucleolar localization of box C/D small nucleolar (sno) RNAs requires the box C/D motif and, in vertebrates, involves transit through Cajal bodies (CB). We report that in yeast, overexpression of a...
Darzacq, Xavier, Jády, Beáta E., Verheggen, Céline, Kiss, Arnold M., Bertrand, Edouard, Kiss, Tamás
Cajal (coiled) bodies are conserved subnuclear organelles that are present in the nucleoplasm of both animal and plant cells. Although Cajal bodies were first described nearly 100 years ago, their...
Mammalian and yeast U3 snoRNPs are matured in specific and related nuclear compartments
Verheggen, Céline, Lafontaine, Denis L.J., Samarsky, Dmitry, Mouaikel, John, Blanchard, Jean-Marie, Bordonné, Rémy, ...
Nucleolar localization of vertebrate box C/D snoRNA involves transit through Cajal bodies, but the significance of this event is unkown. To define better the function of this compartment, we analyzed...
A Cajal body-specific pseudouridylation guide RNA is composed of two box H/ACA snoRNA-like domains
Kiss, Arnold M., Jády, Beáta E., Darzacq, Xavier, Verheggen, Céline, Bertrand, Edouard, Kiss, Tamás
Site-specific post-transcriptional conversion of uridines to pseudouridine in ribosomal RNAs and small nuclear RNAs (snRNAs) is directed by guide RNAs which possess the conserved box H and ACA...
Jády, Beáta E., Darzacq, Xavier, Tucker, Karen E., Matera, A.Gregory, Bertrand, Edouard, Kiss, Tamás
Biogenesis of functional spliceosomal small nuclear RNAs (snRNAs) includes the post-transcriptional covalent modification of numerous internal nucleotides. We have recently demonstrated that...
A common sequence motif determines the Cajal body-specific localization of box H/ACA scaRNAs
Richard, Patricia, Darzacq, Xavier, Bertrand, Edouard, Jády, Beáta E., Verheggen, Céline, Kiss, Tamás
Post-transcriptional synthesis of 2′-O-methylated nucleotides and pseudouridines in Sm spliceosomal small nuclear RNAs takes place in the nucleoplasmic Cajal bodies and it is directed by guide RNAs...
Human let-7 stem–loop precursors harbor features of RNase III cleavage products
Basyuk, Eugenia, Suavet, Florence, Doglio, Alain, Bordonné, Rémy, Bertrand, Edouard
The bidentate RNase III Dicer cleaves microRNA precursors to generate the 21–23 nt long mature RNAs. These precursors are 60–80 nt long, they fold into a characteristic stem–loop structure and...
Nucleolar localization of early tRNA processing
Bertrand, Edouard, Houser-Scott, Felicia, Kendall, Ann, Singer, Robert H., Engelke, David R.
There is little information as to the location of early tRNA biosynthesis. Using fluorescent in situ hybridization in the budding yeast, Saccharomyces cerevisiae, examples of nuclear pre-tRNAs are...
Human Box H/ACA Pseudouridylation Guide RNA Machinery†
Kiss, Arnold M., Jády, Beáta E., Bertrand, Edouard, Kiss, Tamás
Pseudouridine, the most abundant modified nucleoside in RNA, is synthesized by posttranscriptional isomerization of uridines. In eukaryotic RNAs, site-specific synthesis of pseudouridines is directed...
Mouaikel, John, Narayanan, Usha, Verheggen, Céline, Matera, A. Gregory, Bertrand, Edouard, Tazi, Jamal, ...
The biogenesis of spliceosomal small nuclear ribonucleoproteins (snRNPs) requires the cytoplasmic assembly of the Sm-core complex, followed by the hypermethylation of the small nuclear RNA (snRNA)...
An active precursor in assembly of yeast nuclear ribonuclease P.
Srisawat, Chatchawan, Houser-Scott, Felicia, Bertrand, Edouard, Xiao, Shaohua, Singer, Robert H, Engelke, David R
The RNA-protein subunit assembly of nuclear RNase P was investigated by specific isolation and characterization of the precursor and mature forms of RNase P using an RNA affinity ligand. Pre-RNase P...
HENRAS, ANTHONY K., BERTRAND, EDOUARD, CHANFREAU, GUILLAUME
Cleavage of the Saccharomyces cerevisiae primary ribosomal RNA (rRNA) transcript in the 3′ external transcribed spacer (ETS) by Rnt1p generates the 35S pre-rRNA, the earliest detectable species in...
Depletion of SMN by RNA interference in HeLa cells induces defects in Cajal body formation
Girard, Cyrille, Neel, Henry, Bertrand, Edouard, Bordonné, Rémy
Neuronal degeneration in spinal muscular atrophy (SMA) is caused by reduced expression of the survival of motor neuron (SMN) protein. The SMN protein is ubiquitously expressed and is present both in...
Samarsky, Dmitry A., Ferbeyre, Gerardo, Bertrand, Edouard, Singer, Robert H., Cedergren, Robert, Fournier, Maurille J.
A hammerhead ribozyme has been localized to the yeast nucleolus by using the U3 small nucleolar RNA as a carrier. The hybrid small nucleolar RNA:ribozyme, designated a “snorbozyme,” is...
Kendall, Ann, Hull, Melissa W., Bertrand, Edouard, Good, Paul D., Singer, Robert H., Engelke, David R.
In the budding yeast, Saccharomyces cerevisiae, actively transcribed tRNA genes can negatively regulate adjacent RNA polymerase II (pol II)-transcribed promoters. This tRNA gene-mediated silencing is...
King, Thomas H., Decatur, Wayne A., Bertrand, Edouard, Maxwell, E. Stuart, Fournier, Maurille J.
Biogenesis of small nucleolar RNA-protein complexes (snoRNPs) consists of synthesis of the snoRNA and protein components, snoRNP assembly, and localization to the nucleolus. Recently, two...
Alkaline fixation drastically improves the signal of in situ hybridization
Basyuk, Eugenia, Bertrand, Edouard, Journot, Laurent
In situ hybridization (ISH) is widely used to detect DNA and RNA sequences within the cell and tissue sections. The important step in performing this technique is tissue fixation. We investigated the...
Monitoring Retroviral RNA Dimerization In Vivo via Hammerhead Ribozyme Cleavage
Pal, Bijay K., Scherer, Lisa, Zelby, Laurie, Bertrand, Edouard, Rossi, John J.
We have used a strategy for colocalization of Psi (Ψ)-tethered ribozymes and targets to demonstrate that Ψ sequences are capable of specific interaction in the cytoplasm of both packaging and...
Verheggen, Céline, Mouaikel, John, Thiry, Marc, Blanchard, Jean-Marie, Tollervey, David, Bordonné, Remi, ...
Nucleolar localization of box C/D small nucleolar (sno) RNAs requires the box C/D motif and, in vertebrates, involves transit through Cajal bodies (CB). We report that in yeast, overexpression of a...
Darzacq, Xavier, Jády, Beáta E., Verheggen, Céline, Kiss, Arnold M., Bertrand, Edouard, Kiss, Tamás
Cajal (coiled) bodies are conserved subnuclear organelles that are present in the nucleoplasm of both animal and plant cells. Although Cajal bodies were first described nearly 100 years ago, their...
Mammalian and yeast U3 snoRNPs are matured in specific and related nuclear compartments
Verheggen, Céline, Lafontaine, Denis L.J., Samarsky, Dmitry, Mouaikel, John, Blanchard, Jean-Marie, Bordonné, Rémy, ...
Nucleolar localization of vertebrate box C/D snoRNA involves transit through Cajal bodies, but the significance of this event is unkown. To define better the function of this compartment, we analyzed...
A Cajal body-specific pseudouridylation guide RNA is composed of two box H/ACA snoRNA-like domains
Kiss, Arnold M., Jády, Beáta E., Darzacq, Xavier, Verheggen, Céline, Bertrand, Edouard, Kiss, Tamás
Site-specific post-transcriptional conversion of uridines to pseudouridine in ribosomal RNAs and small nuclear RNAs (snRNAs) is directed by guide RNAs which possess the conserved box H and ACA...
Jády, Beáta E., Darzacq, Xavier, Tucker, Karen E., Matera, A.Gregory, Bertrand, Edouard, Kiss, Tamás
Biogenesis of functional spliceosomal small nuclear RNAs (snRNAs) includes the post-transcriptional covalent modification of numerous internal nucleotides. We have recently demonstrated that...
A common sequence motif determines the Cajal body-specific localization of box H/ACA scaRNAs
Richard, Patricia, Darzacq, Xavier, Bertrand, Edouard, Jády, Beáta E., Verheggen, Céline, Kiss, Tamás
Post-transcriptional synthesis of 2′-O-methylated nucleotides and pseudouridines in Sm spliceosomal small nuclear RNAs takes place in the nucleoplasmic Cajal bodies and it is directed by guide RNAs...
Human let-7 stem–loop precursors harbor features of RNase III cleavage products
Basyuk, Eugenia, Suavet, Florence, Doglio, Alain, Bordonné, Rémy, Bertrand, Edouard
The bidentate RNase III Dicer cleaves microRNA precursors to generate the 21–23 nt long mature RNAs. These precursors are 60–80 nt long, they fold into a characteristic stem–loop structure and...
Nucleolar localization of early tRNA processing
Bertrand, Edouard, Houser-Scott, Felicia, Kendall, Ann, Singer, Robert H., Engelke, David R.
There is little information as to the location of early tRNA biosynthesis. Using fluorescent in situ hybridization in the budding yeast, Saccharomyces cerevisiae, examples of nuclear pre-tRNAs are...
Human Box H/ACA Pseudouridylation Guide RNA Machinery†
Kiss, Arnold M., Jády, Beáta E., Bertrand, Edouard, Kiss, Tamás
Pseudouridine, the most abundant modified nucleoside in RNA, is synthesized by posttranscriptional isomerization of uridines. In eukaryotic RNAs, site-specific synthesis of pseudouridines is directed...
Mouaikel, John, Narayanan, Usha, Verheggen, Céline, Matera, A. Gregory, Bertrand, Edouard, Tazi, Jamal, ...
The biogenesis of spliceosomal small nuclear ribonucleoproteins (snRNPs) requires the cytoplasmic assembly of the Sm-core complex, followed by the hypermethylation of the small nuclear RNA (snRNA)...
Cell Cycle-dependent Recruitment of Telomerase RNA and Cajal Bodies to Human Telomeres
Jády, Beáta E., Richard, Patricia, Bertrand, Edouard, Kiss, Tamás
Telomerase is a ribonucleoprotein enzyme that counteracts replicative telomere erosion by adding telomeric sequence repeats onto chromosome ends. Despite its well-established role in telomere...
An active precursor in assembly of yeast nuclear ribonuclease P.
Srisawat, Chatchawan, Houser-Scott, Felicia, Bertrand, Edouard, Xiao, Shaohua, Singer, Robert H, Engelke, David R
The RNA-protein subunit assembly of nuclear RNase P was investigated by specific isolation and characterization of the precursor and mature forms of RNase P using an RNA affinity ligand. Pre-RNase P...
HENRAS, ANTHONY K., BERTRAND, EDOUARD, CHANFREAU, GUILLAUME
Cleavage of the Saccharomyces cerevisiae primary ribosomal RNA (rRNA) transcript in the 3′ external transcribed spacer (ETS) by Rnt1p generates the 35S pre-rRNA, the earliest detectable species in...
Depletion of SMN by RNA interference in HeLa cells induces defects in Cajal body formation
Girard, Cyrille, Neel, Henry, Bertrand, Edouard, Bordonné, Rémy
Neuronal degeneration in spinal muscular atrophy (SMA) is caused by reduced expression of the survival of motor neuron (SMN) protein. The SMN protein is ubiquitously expressed and is present both in...
A real-time view of the TAR:Tat:P-TEFb complex at HIV-1 transcription sites
Molle, Dorothée, Maiuri, Paolo, Boireau, Stéphanie, Bertrand, Edouard, Knezevich, Anna, Marcello, Alessandro, ...
HIV-1 transcription is tightly regulated: silent in long-term latency and highly active in acutely-infected cells. Transcription is activated by the viral protein Tat, which recruits the elongation...
The Clathrin Adaptor Complex AP-1 Binds HIV-1 and MLV Gag and Facilitates Their Budding
Camus, Grégory, Segura-Morales, Carolina, Molle, Dorothee, Lopez-Vergès, Sandra, Begon-Pescia, Christina, Cazevieille, Chantal, ...
Retroviral assembly is driven by Gag, and nascent viral particles escape cells by recruiting the machinery that forms intralumenal vesicles of multivesicular bodies. In this study, we show that the...
Bsr, a Nuclear-retained RNA with Monoallelic Expression
Royo, Hélène, Basyuk, Eugenia, Marty, Virginie, Marques, Maud, Bertrand, Edouard, Cavaillé, Jérôme
The imprinted Dlk1-Gtl2 and Prader-Willi syndrome (PWS) regions are characterized by a complex noncoding transcription unit spanning arrays of tandemly repeated C/D RNA genes. These noncoding RNAs...
Chéné, Isaure Du, Basyuk, Euguenia, Lin, Yea-Lih, Triboulet, Robinson, Knezevich, Anna, Chable-Bessia, Christine, ...
HIV-1 gene expression is the major determinant regulating the rate of virus replication and, consequently, AIDS progression. Following primary infection, most infected cells produce virus. However, a...
A Dynamic Scaffold of Pre-snoRNP Factors Facilitates Human Box C/D snoRNP Assembly▿
McKeegan, Kenneth Scott, Debieux, Charles Maurice, Boulon, Séverine, Bertrand, Edouard, Watkins, Nicholas James
The box C/D small nucleolar RNPs (snoRNPs) are essential for the processing and modification of rRNA. The core box C/D proteins are restructured during human U3 box C/D snoRNP biogenesis; however,...
ADAR2-mediated editing of RNA substrates in the nucleolus is inhibited by C/D small nucleolar RNAs
Vitali, Patrice, Basyuk, Eugenia, Le Meur, Elodie, Bertrand, Edouard, Muscatelli, Françoise, Cavaillé, Jérôme, ...
Posttranscriptional, site-specific adenosine to inosine (A-to-I) base conversions, designated as RNA editing, play significant roles in generating diversity of gene expression. However, little is...
Human telomerase RNA and box H/ACA scaRNAs share a common Cajal body–specific localization signal
Jády, Beáta E., Bertrand, Edouard, Kiss, Tamás
Telomerase is a ribonucleoprotein reverse transcriptase that uses its RNA component as a template for synthesis of telomeric DNA repeats at the ends of linear eukaryotic chromosomes. Here,...
Durand, Sébastien, Cougot, Nicolas, Mahuteau-Betzer, Florence, Nguyen, Chi-Hung, Grierson, David S., Bertrand, Edouard, ...
In mammals, nonsense-mediated mRNA decay (NMD) is a quality-control mechanism that degrades mRNA harboring a premature termination codon to prevent the synthesis of truncated proteins. To gain...
The transcriptional cycle of HIV-1 in real-time and live cells
Boireau, Stéphanie, Maiuri, Paolo, Basyuk, Eugenia, De La Mata, Manuel, Knezevich, Anna, Pradet-Balade, Bérangère, ...
RNA polymerase II (RNAPII) is a fundamental enzyme, but few studies have analyzed its activity in living cells. Using human immunodeficiency virus (HIV) type 1 reporters, we study real-time messenger...
The RasGAP-associated endoribonuclease G3BP assembles stress granules
Tourrière, Hélène, Chebli, Karim, Zekri, Latifa, Courselaud, Brice, Blanchard, Jean Marie, Bertrand, Edouard, ...
Stress granules (SGs) are formed in the cytoplasm in response to various toxic agents, and are believed to play a critical role in the regulation of mRNA metabolism during stress. In SGs, mRNAs are...
A Novel Role for PA28γ-Proteasome in Nuclear Speckle Organization and SR Protein Trafficking
Baldin, Véronique, Militello, Muriel, Thomas, Yann, Doucet, Christine, Fic, Weronika, Boireau, Stephanie, ...
In eukaryotic cells, proteasomes play an essential role in intracellular proteolysis and are involved in the control of most biological processes through regulated degradation of key proteins....
The Hsp90 chaperone controls the biogenesis of L7Ae RNPs through conserved machinery
Boulon, Séverine, Marmier-Gourrier, Nathalie, Pradet-Balade, Bérengère, Wurth, Laurence, Verheggen, Céline, Jády, Beáta E., ...
RNA-binding proteins of the L7Ae family are at the heart of many essential ribonucleoproteins (RNPs), including box C/D and H/ACA small nucleolar RNPs, U4 small nuclear RNP, telomerase, and messenger...