Loss of HMW1 and HMW3 in noncytadhering mutants of Mycoplasma pneumoniae occurs post-translationally
Popham, Phillip L., Hahn, Tae-Wook, Krebes, Kathleen A., Krause, Duncan C.
The genomic sequence of Mycoplasma pneumoniae establish this cell-wall-less prokaryote as among the smallest known microorganisms capable of self-replication. However, this genomic simplicity and...
Romero-Arroyo, Cynthia E., Jordan, Jarrat, Peacock, Susan J., Willby, Melisa J., Farmer, Mark A., Krause, Duncan C.
The attachment organelle of Mycoplasma pneumoniae is a polar, tapered cell extension containing an intracytoplasmic, electron-dense core. This terminal structure is the leading end in gliding...
Fisseha, Makda, Göhlmann, Hinrich W. H., Herrmann, Richard, Krause, Duncan C.
Mycoplasma pneumoniae cytadherence is mediated by a specialized, polar attachment organelle. Certain spontaneously arising cytadherence mutants (designated class I) lack HMW2, fail to localize the...
Transcriptional Analysis of the hmw Gene Cluster of Mycoplasma pneumoniae
Waldo, Robert H., Popham, Phillip L., Romero-Arroyo, Cynthia E., Mothershed, Elizabeth A., Lee, Kyungok K., Krause, Duncan C.
Mycoplasma pneumoniae adherence to host cells is a multifactorial process that requires the cytadhesin P1 and additional accessory proteins. The hmw gene cluster consists of the genes p30, hmw3, and...
Balish, Mitchell F., Hahn, Tae-Wook, Popham, Phillip L., Krause, Duncan C.
Mycoplasma pneumoniae adsorbs to host respiratory epithelium primarily by its attachment organelle, the proper function of which depends upon mycoplasma adhesin and cytoskeletal proteins. Among the...
Jordan, Jarrat L., Berry, Karen M., Balish, Mitchell F., Krause, Duncan C.
The surface protein P65 is a constituent of the Mycoplasma pneumoniae cytoskeleton and is present at reduced levels in mutants lacking the cytadherence accessory protein HMW2. Pulse-chase studies...
HMW1 Is Required for Cytadhesin P1 Trafficking to the Attachment Organelle in Mycoplasma pneumoniae
Hahn, Tae-Wook, Willby, Melisa J., Krause, Duncan C.
Mycoplasma pneumoniae proteins HMW1-HMW3 collectively are essential for cytadherence, but the function or requirement for each has not been defined. Cytadherence mutant M6 lacks HMW1 because of a...
Bartonella henselae Invasion of Feline Erythrocytes In Vitro
Mehock, Jane R., Greene, Craig E., Gherardini, Frank C., Hahn, Tae-Wook, Krause, Duncan C.
Bartonella henselae, the causative agent of cat scratch disease, establishes long-term bacteremia in cats, in which it attaches to and invades feline erythrocytes (RBC). Feline RBC invasion was...
Willby, Melisa J., Krause, Duncan C.
The proteins required for adherence of the pathogen Mycoplasma pneumoniae to host respiratory epithelial cells are localized to a polar structure, the attachment organelle. A number of these proteins...
Reacquisition of Specific Proteins Confers Virulence in Mycoplasma pneumoniae
Krause, Duncan C., Leith, Debra K., Baseman, Joel B.
Hemadsorbing revertants were isolated from spontaneous hemadsorption-negative, avirulent mutants of Mycoplasma pneumoniae. The revertants simultaneously reacquired specific proteins absent in their...
Krause, Duncan C., Baseman, Joel B.
Antiserum and purified immunoglobulin directed against Mycoplasma pneumoniae membrane protein P1 were examined for their influence on mycoplasma viability, metabolism, and cytadsorption. Anti-P1...
Predominant Outer Membrane Antigens of Bartonella henselae
Chenoweth, Matthew R., Greene, Craig E., Krause, Duncan C., Gherardini, Frank C.
A hallmark of Bartonella henselae is persistent bacteremia in cats despite the presence of a vigorous host immune response. To understand better the long-term survival of B. henselae in cats, we...
Willby, Melisa J., Balish, Mitchell F., Ross, Stephanie M., Lee, Kyungok K., Jordan, Jarrat L., Krause, Duncan C.
The cytoskeletal proteins HMW1 and HMW2 are components of the terminal organelle of the cell wall-less bacterium Mycoplasma pneumoniae. HMW1 is required for a tapered, filamentous morphology but...
Waldo, Robert H., Jordan, Jarrat L., Krause, Duncan C.
A mutation in gene MPN142 (orf6) was identified in the Mycoplasma pneumoniae cytadherence mutant III-4. MPN142 encodes virulence-specific proteins P90 and P40 (proteins B and C, respectively)....
Hasselbring, Benjamin M., Jordan, Jarrat L., Krause, Duncan C.
The cell-wall-less prokaryote Mycoplasma pneumoniae, long considered among the smallest and simplest cells capable of self-replication, has a distinct cellular polarity characterized by the presence...
Waldo, Robert H., Krause, Duncan C.
The genes MPN141 and MPN142 encode the major adhesin P1 and the cytadherence-related B/C proteins (P90/P40), respectively, in Mycoplasma pneumoniae. Using reverse transcriptase PCR we found open...
Loss of HMW1 and HMW3 in noncytadhering mutants of Mycoplasma pneumoniae occurs post-translationally
Popham, Phillip L., Hahn, Tae-Wook, Krebes, Kathleen A., Krause, Duncan C.
The genomic sequence of Mycoplasma pneumoniae establish this cell-wall-less prokaryote as among the smallest known microorganisms capable of self-replication. However, this genomic simplicity and...
Romero-Arroyo, Cynthia E., Jordan, Jarrat, Peacock, Susan J., Willby, Melisa J., Farmer, Mark A., Krause, Duncan C.
The attachment organelle of Mycoplasma pneumoniae is a polar, tapered cell extension containing an intracytoplasmic, electron-dense core. This terminal structure is the leading end in gliding...
Fisseha, Makda, Göhlmann, Hinrich W. H., Herrmann, Richard, Krause, Duncan C.
Mycoplasma pneumoniae cytadherence is mediated by a specialized, polar attachment organelle. Certain spontaneously arising cytadherence mutants (designated class I) lack HMW2, fail to localize the...
Transcriptional Analysis of the hmw Gene Cluster of Mycoplasma pneumoniae
Waldo, Robert H., Popham, Phillip L., Romero-Arroyo, Cynthia E., Mothershed, Elizabeth A., Lee, Kyungok K., Krause, Duncan C.
Mycoplasma pneumoniae adherence to host cells is a multifactorial process that requires the cytadhesin P1 and additional accessory proteins. The hmw gene cluster consists of the genes p30, hmw3, and...
Balish, Mitchell F., Hahn, Tae-Wook, Popham, Phillip L., Krause, Duncan C.
Mycoplasma pneumoniae adsorbs to host respiratory epithelium primarily by its attachment organelle, the proper function of which depends upon mycoplasma adhesin and cytoskeletal proteins. Among the...
Jordan, Jarrat L., Berry, Karen M., Balish, Mitchell F., Krause, Duncan C.
The surface protein P65 is a constituent of the Mycoplasma pneumoniae cytoskeleton and is present at reduced levels in mutants lacking the cytadherence accessory protein HMW2. Pulse-chase studies...
HMW1 Is Required for Cytadhesin P1 Trafficking to the Attachment Organelle in Mycoplasma pneumoniae
Hahn, Tae-Wook, Willby, Melisa J., Krause, Duncan C.
Mycoplasma pneumoniae proteins HMW1-HMW3 collectively are essential for cytadherence, but the function or requirement for each has not been defined. Cytadherence mutant M6 lacks HMW1 because of a...
Bartonella henselae Invasion of Feline Erythrocytes In Vitro
Mehock, Jane R., Greene, Craig E., Gherardini, Frank C., Hahn, Tae-Wook, Krause, Duncan C.
Bartonella henselae, the causative agent of cat scratch disease, establishes long-term bacteremia in cats, in which it attaches to and invades feline erythrocytes (RBC). Feline RBC invasion was...
Willby, Melisa J., Krause, Duncan C.
The proteins required for adherence of the pathogen Mycoplasma pneumoniae to host respiratory epithelial cells are localized to a polar structure, the attachment organelle. A number of these proteins...
Reacquisition of Specific Proteins Confers Virulence in Mycoplasma pneumoniae
Krause, Duncan C., Leith, Debra K., Baseman, Joel B.
Hemadsorbing revertants were isolated from spontaneous hemadsorption-negative, avirulent mutants of Mycoplasma pneumoniae. The revertants simultaneously reacquired specific proteins absent in their...
Krause, Duncan C., Baseman, Joel B.
Antiserum and purified immunoglobulin directed against Mycoplasma pneumoniae membrane protein P1 were examined for their influence on mycoplasma viability, metabolism, and cytadsorption. Anti-P1...
Predominant Outer Membrane Antigens of Bartonella henselae
Chenoweth, Matthew R., Greene, Craig E., Krause, Duncan C., Gherardini, Frank C.
A hallmark of Bartonella henselae is persistent bacteremia in cats despite the presence of a vigorous host immune response. To understand better the long-term survival of B. henselae in cats, we...
Willby, Melisa J., Balish, Mitchell F., Ross, Stephanie M., Lee, Kyungok K., Jordan, Jarrat L., Krause, Duncan C.
The cytoskeletal proteins HMW1 and HMW2 are components of the terminal organelle of the cell wall-less bacterium Mycoplasma pneumoniae. HMW1 is required for a tapered, filamentous morphology but...
Waldo, Robert H., Jordan, Jarrat L., Krause, Duncan C.
A mutation in gene MPN142 (orf6) was identified in the Mycoplasma pneumoniae cytadherence mutant III-4. MPN142 encodes virulence-specific proteins P90 and P40 (proteins B and C, respectively)....
Hasselbring, Benjamin M., Jordan, Jarrat L., Krause, Duncan C.
The cell-wall-less prokaryote Mycoplasma pneumoniae, long considered among the smallest and simplest cells capable of self-replication, has a distinct cellular polarity characterized by the presence...
Waldo, Robert H., Krause, Duncan C.
The genes MPN141 and MPN142 encode the major adhesin P1 and the cytadherence-related B/C proteins (P90/P40), respectively, in Mycoplasma pneumoniae. Using reverse transcriptase PCR we found open...
Transposon Mutagenesis Identifies Genes Associated with Mycoplasma pneumoniae Gliding Motility
Hasselbring, Benjamin M., Page, Clinton A., Sheppard, Edward S., Krause, Duncan C.
The wall-less prokaryote Mycoplasma pneumoniae, a common cause of chronic respiratory tract infections in humans, is considered to be among the smallest and simplest known cells capable of...
Terminal organelle development in the cell wall-less bacterium Mycoplasma pneumoniae
Hasselbring, Benjamin M., Jordan, Jarrat L., Krause, Robert W., Krause, Duncan C.
Mycoplasmas are cell wall-less bacteria considered among the smallest and simplest prokaryotes known, and yet several species including Mycoplasma pneumoniae have a remarkably complex cellular...
Jordan, Jarrat L., Chang, How-Yi, Balish, Mitchell F., Holt, Lynley S., Bose, Stephanie R., Hasselbring, Benjamin M., ...
Mycoplasma pneumoniae protein P200 was localized to the terminal organelle, which functions in cytadherence and gliding motility. The loss of P200 had no impact on binding to erythrocytes and A549...
Transposon Mutagenesis Identifies Genes Associated with Mycoplasma pneumoniae Gliding Motility
Hasselbring, Benjamin M., Page, Clinton A., Sheppard, Edward S., Krause, Duncan C.
Hasselbring, Benjamin M., Krause, Duncan C.
Mycoplasma pneumoniae is a major cause of bronchitis and atypical pneumonia in humans. This cell wall-less bacterium has a complex terminal organelle that functions in cytadherence and gliding...