An, Dong Sung, Donahue, Robert E., Kamata, Masakazu, Poon, Betty, Metzger, Mark, Mao, Si-Hua, ...
RNAi is a powerful method for suppressing gene expression that has tremendous potential for therapeutic applications. However, because endogenous RNAi plays a role in normal cellular functions,...
Qin, Xiao-Feng, An, Dong Sung, Chen, Irvin S. Y., Baltimore, David
Double-stranded RNAs approximate to 21 nucleotides long [small interfering RNA (siRNA)] are recognized as powerful reagents to reduce the expression of specific genes. To use them as reagents to...
An, Dong Sung, Morizono, Kouki, Li, Qi-Xiang, Mao, Si Hua, Lu, Stephanie, Chen, Irvin S. Y.
Recently, gene therapy vectors based upon the human immunodeficiency virus type 1 (HIV-1) genome have been developed. Here, we create an HIV-1 vector which is defective for all HIV-1 genes, but which...
An, Dong Sung, Wersto, Robert P., Agricola, Brian A., Metzger, Mark E., Lu, Stephanie, Amado, Rafael G., ...
Recently, gene delivery vectors based on human immunodeficiency virus (HIV) have been developed as an alternative mode of gene delivery. These vectors have a number of advantages, particularly in...
Kung, Sam K. P., An, Dong Sung, Chen, Irvin S. Y.
We constructed human immunodeficiency virus type 1 (HIV-1) vectors that will allow higher levels of gene expression in T cells. Gene expression under the control of an internal cytomegalovirus (CMV)...
Human Immunodeficiency Virus Env-Independent Infection of Human CD4− Cells
Pang, Shen, Yu, Duan, An, Dong Sung, Baldwin, Gayle C., Xie, Yiming, Poon, Betty, ...
CD4− epithelial cells covering mucosal surfaces serve as the primary barrier to prevent human immunodeficiency virus type 1 (HIV-1) infection. We used HIV-1 vectors carrying the enhanced green...
Envelope Gene of the Human Endogenous Retrovirus HERV-W Encodes a Functional Retrovirus Envelope
An, Dong Sung, Xie, Yi-ming, Chen, Irvin S. Y.
A member of the human endogenous retrovirus (HERV) family termed HERV-W encodes a highly fusogenic membrane glycoprotein that appears to be expressed specifically in the placenta. It is unclear...
An, Dong Sung, Kung, Sam K. P., Bonifacino, Aylin, Wersto, Robert P., Metzger, Mark E., Agricola, Brian A., ...
Nonhuman primate model systems of autologous CD34+ cell transplant are the most effective means to assess the safety and capabilities of lentivirus vectors. Toward this end, we tested the efficiency...
Qin, Xiao-Feng, An, Dong Sung, Chen, Irvin S. Y., Baltimore, David
Double-stranded RNAs ≈21 nucleotides long [small interfering RNA (siRNA)] are recognized as powerful reagents to reduce the expression of specific genes. To use them as reagents to protect cells...
The Ataxia Telangiectasia-Mutated and Rad3-Related Protein Is Dispensable for Retroviral Integration
DeHart, Jason L., Andersen, Joshua L., Zimmerman, Erik S., Ardon, Orly, An, Dong Sung, Blackett, Jana, ...
Integration into the host cell DNA is an essential part of the retroviral life cycle and is required for the productive replication of a retrovirus. Retroviral integration involves cleavage of the...
Lentivirus-delivered stable gene silencing by RNAi in primary cells
STEWART, SHEILA A., DYKXHOORN, DEREK M., PALLISER, DEBORAH, MIZUNO, HANA, YU, EVAN Y., AN, DONG SUNG, ...
Genome-wide genetic approaches have proven useful for examining pathways of biological significance in model organisms such as Saccharomyces cerevisiae, Drosophila melanogastor, and Caenorhabditis...
Dykes, Carrie, Wang, Jiong, Jin, Xia, Planelles, Vicente, An, Dong Sung, Tallo, Amanda, ...
Human immunodeficiency virus type 1 (HIV-1) replication efficiency or fitness, as measured in cell culture, has been postulated to correlate with clinical outcome of HIV infection, although this is...
An, Dong Sung, Morizono, Kouki, Li, Qi-Xiang, Mao, Si Hua, Lu, Stephanie, Chen, Irvin S. Y.
Recently, gene therapy vectors based upon the human immunodeficiency virus type 1 (HIV-1) genome have been developed. Here, we create an HIV-1 vector which is defective for all HIV-1 genes, but which...
An, Dong Sung, Wersto, Robert P., Agricola, Brian A., Metzger, Mark E., Lu, Stephanie, Amado, Rafael G., ...
Recently, gene delivery vectors based on human immunodeficiency virus (HIV) have been developed as an alternative mode of gene delivery. These vectors have a number of advantages, particularly in...
Kung, Sam K. P., An, Dong Sung, Chen, Irvin S. Y.
We constructed human immunodeficiency virus type 1 (HIV-1) vectors that will allow higher levels of gene expression in T cells. Gene expression under the control of an internal cytomegalovirus (CMV)...
Human Immunodeficiency Virus Env-Independent Infection of Human CD4− Cells
Pang, Shen, Yu, Duan, An, Dong Sung, Baldwin, Gayle C., Xie, Yiming, Poon, Betty, ...
CD4− epithelial cells covering mucosal surfaces serve as the primary barrier to prevent human immunodeficiency virus type 1 (HIV-1) infection. We used HIV-1 vectors carrying the enhanced green...
Envelope Gene of the Human Endogenous Retrovirus HERV-W Encodes a Functional Retrovirus Envelope
An, Dong Sung, Xie, Yi-ming, Chen, Irvin S. Y.
A member of the human endogenous retrovirus (HERV) family termed HERV-W encodes a highly fusogenic membrane glycoprotein that appears to be expressed specifically in the placenta. It is unclear...
An, Dong Sung, Kung, Sam K. P., Bonifacino, Aylin, Wersto, Robert P., Metzger, Mark E., Agricola, Brian A., ...
Nonhuman primate model systems of autologous CD34+ cell transplant are the most effective means to assess the safety and capabilities of lentivirus vectors. Toward this end, we tested the efficiency...
Qin, Xiao-Feng, An, Dong Sung, Chen, Irvin S. Y., Baltimore, David
Double-stranded RNAs ≈21 nucleotides long [small interfering RNA (siRNA)] are recognized as powerful reagents to reduce the expression of specific genes. To use them as reagents to protect cells...
The Ataxia Telangiectasia-Mutated and Rad3-Related Protein Is Dispensable for Retroviral Integration
DeHart, Jason L., Andersen, Joshua L., Zimmerman, Erik S., Ardon, Orly, An, Dong Sung, Blackett, Jana, ...
Integration into the host cell DNA is an essential part of the retroviral life cycle and is required for the productive replication of a retrovirus. Retroviral integration involves cleavage of the...
Lentivirus-delivered stable gene silencing by RNAi in primary cells
STEWART, SHEILA A., DYKXHOORN, DEREK M., PALLISER, DEBORAH, MIZUNO, HANA, YU, EVAN Y., AN, DONG SUNG, ...
Genome-wide genetic approaches have proven useful for examining pathways of biological significance in model organisms such as Saccharomyces cerevisiae, Drosophila melanogastor, and Caenorhabditis...
Dykes, Carrie, Wang, Jiong, Jin, Xia, Planelles, Vicente, An, Dong Sung, Tallo, Amanda, ...
Human immunodeficiency virus type 1 (HIV-1) replication efficiency or fitness, as measured in cell culture, has been postulated to correlate with clinical outcome of HIV infection, although this is...
An, Dong Sung, Poon, Betty, Fang, Raphael Ho Tsong, Weijer, Kees, Blom, Bianca, Spits, Hergen, ...
The goal of this study was to develop a small-animal model to study human immunodeficiency virus type 1 (HIV-1) pathogenesis in blood and primary and secondary lymphoid organs. Rag2−/−γc−/−...
Stable reduction of CCR5 by RNAi through hematopoietic stem cell transplant in non-human primates
An, Dong Sung, Donahue, Robert E., Kamata, Masakazu, Poon, Betty, Metzger, Mark, Mao, Si-Hua, ...
RNAi is a powerful method for suppressing gene expression that has tremendous potential for therapeutic applications. However, because endogenous RNAi plays a role in normal cellular functions,...