Polycomb Repressive Complex 2 (PRC2) Restricts Hematopoietic Stem Cell Activity (2008)
Ian J. Majewski, Marnie E. Blewitt, Edward J. McManus, Melanie Bahlo, Adrienne A. Hilton, ...
Polycomb group proteins are transcriptional repressors that play a central role in the establishment and maintenance of gene expression patterns during development. Using mice with an...
Pohl, Thomas, Gugasyan, Raffi, Grumont, Raelene J., Strasser, Andreas, Metcalf, Donald, Tarlinton, David, ...
Transcription factors NF-KB1 and c-Rel, individually dispensable during embryogenesis, serve similar, yet distinct, roles in the function of mature hemopoietic cells. Redundancy among Rel/ NF-KB...
Grossmann, Mathis, Metcalf, Donald, Merryfull, Julie, Beg, Amer, Baltimore, David, Gerondakis, Steve
Individual Rel/NF-kappa B transcription factors, although dispensable for the development and maturation of most hemopoietic cells, are critical regulators of normal immune function. Redundancy among...
Sparrow, Lindsay G., Metcalf, Donald, Hunkapiller, Michael W., Hood, Leroy E., Burgess, Anthony W.
A procedure utilizing reversed-phase high-performance liquid chromatography is described for the purification of asialo granulocyte--macrophage colony stimulating factor (asialo-GM-CSF) from mouse...
Sparrow, Lindsay G., Metcalf, Donald, Hunkapiller, Michael W., Hood, Leroy E., Burgess, Anthony W.
A procedure utilizing reversed-phase high-performance liquid chromatography is described for the purification of asialo granulocyte--macrophage colony stimulating factor (asialo-GM-CSF) from mouse...
Metcalf, Donald, Di Rago, Ladina, Mifsud, Sandra, Hartley, Lynne, Alexander, Warren S.
Mice lacking the gene encoding the suppressor of cytokine signaling-1 (SOCS-1 −/−) and heterozygous for the IFN–γ gene (IFN-γ +/−) avoided the IFN-γ-dependent preweaning death of SOCS-1...
Twenty proteins containing a C-terminal SOCS box form five structural classes
Hilton, Douglas J., Richardson, Rachael T., Alexander, Warren S., Viney, Elizabeth M., Willson, Tracy A., Sprigg, Naomi S., ...
The four members of the recently identified suppressor of cytokines signaling family (SOCS-1, SOCS-2, SOCS-3, and CIS, where CIS is cytokine-inducible SH2-containing protein) appear, by various...
Grossmann, Mathis, Metcalf, Donald, Merryfull, Julie, Beg, Amer, Baltimore, David, Gerondakis, Steve
Individual Rel/NF-κB transcription factors, although dispensable for the development and maturation of most hemopoietic cells, are critical regulators of normal immune function. Redundancy among...
Nicholson, Sandra E., De Souza, David, Fabri, Louis J., Corbin, Jason, Willson, Tracy A., Zhang, Jian-Guo, ...
Suppressor of cytokine signaling-3 (SOCS-3) is one member of a family of intracellular inhibitors of signaling pathways initiated by cytokines that use, among others, the common receptor subunit...
Hematopoietic stem cell deficiencies in mice lacking c-Mpl, the receptor for thrombopoietin
Kimura, Shinya, Roberts, Andrew W., Metcalf, Donald, Alexander, Warren S.
Thrombopoietin (TPO) acts as a lineage-specific late-acting factor to stimulate megakaryocyte and platelet formation. However, analysis of mice lacking either the cytokine or its receptor, c-Mpl,...
Elefanty, Andrew G., Begley, C. Glenn, Metcalf, Donald, Barnett, Louise, Köntgen, Frank, Robb, Lorraine
Gene targeting experiments have demonstrated that the transcription factor SCL is essential for primitive and definitive hematopoiesis in the mouse. To study the functional properties of...
Apoptosis regulator Bcl-w is essential for spermatogenesis but appears otherwise redundant
Print, Cristin G., Loveland, Kate L., Gibson, Leonie, Meehan, Terri, Stylianou, Anna, Wreford, Nigel, ...
Proteins of the Bcl-2 family are important regulators of apoptosis in many tissues of the embryo and adult. The recently isolated bcl-w gene encodes a pro-survival member of the Bcl-2 family, which...
The stimulation by Flk2-ligand (FL) of blast colony formation by murine bone marrow cells was selectively potentiated by the addition of regulators sharing in common the gp130 signaling...
Liver degeneration and lymphoid deficiencies in mice lacking suppressor of cytokine signaling-1
Starr, Robyn, Metcalf, Donald, Elefanty, Andrew G., Brysha, Marta, Willson, Tracy A., Nicola, Nicos A., ...
SOCS-1, a member of the suppressor of cytokine signaling (SOCS) family, was identified in a genetic screen for inhibitors of interleukin 6 signal transduction. SOCS-1 transcription is induced by...
Ogilvy, Sarah, Metcalf, Donald, Print, Cristin G., Bath, Mary L., Harris, Alan W., Adams, Jerry M.
Bcl-2, which can both reduce apoptosis and retard cell cycle entry, is thought to have important roles in hematopoiesis. To evaluate the impact of its ubiquitous overexpression within this system, we...
Leptin can induce proliferation, differentiation, and functional activation of hemopoietic cells
Gainsford, Timothy, Willson, Tracy A., Metcalf, Donald, Handman, Emanuela, McFarlane, Clare, Ng, Ashley, ...
Many cytokines exert their biological effect through members of the hemopoietin receptor family. Using degenerate oligonucleotides to the common WSXWS motif, we have cloned from human hemopoietic...
Zhang, Jian-Guo, Farley, Alison, Nicholson, Sandra E., Willson, Tracy A., Zugaro, Lisa M., Simpson, Richard J., ...
The suppressors of cytokine signaling (SOCS) family of proteins act as intracellular inhibitors of several cytokine signal transduction pathways. Their expression is induced by cytokine activation of...
Normal mouse marrow cells were stimulated by stem cell factor (SCF) to form dispersed or multicentric blast colonies containing progenitor cells committed to various hematopoietic lineages....
Placental defects and embryonic lethality in mice lacking suppressor of cytokine signaling 3
Roberts, Andrew W., Robb, Lorraine, Rakar, Steven, Hartley, Lynne, Cluse, Leonie, Nicola, Nicos A., ...
Mice lacking suppressor of cytokine signaling 3 (SOCS3) exhibited embryonic lethality with death occurring between days 11 and 13 of gestation. At this stage, SOCS3−/− embryos were slightly...
Zhang, Jian-Guo, Metcalf, Donald, Rakar, Steven, Asimakis, Maria, Greenhalgh, Christopher J., Willson, Tracy A., ...
Suppressor of Cytokine Signaling-1 (SOCS-1) is an essential physiological inhibitor of IFN-γ signaling. Mice lacking this gene die in the early postnatal period from a disease characterized by...
Functional Analysis of Asb-1 Using Genetic Modification in Mice
Kile, Benjamin T., Metcalf, Donald, Mifsud, Sandra, DiRago, Ladina, Nicola, Nicos A., Hilton, Douglas J., ...
The Asbs are a family of ankyrin repeat proteins that, along with four other protein families, contain a C-terminal SOCS box motif, which was first identified in the suppressor of cytokine signaling...
Metcalf, Donald, Mifsud, Sandra, Di Rago, Ladina, Nicola, Nicos A., Hilton, Douglas J., Alexander, Warren S.
Mice with inactivation of the gene encoding the suppressor of cytokine signaling-1 (SOCS-1) die in neonatal life with an IFN-γ-dependent inflammatory disease dominated by fatty degeneration and...
Pohl, Thomas, Gugasyan, Raffi, Grumont, Raelene J., Strasser, Andreas, Metcalf, Donald, Tarlinton, David, ...
Transcription factors NF-κB1 and c-Rel, individually dispensable during embryogenesis, serve similar, yet distinct, roles in the function of mature hemopoietic cells. Redundancy among Rel/NF-κB...
Krebs, Danielle L., Uren, Rachel T., Metcalf, Donald, Rakar, Steven, Zhang, Jian-Guo, Starr, Robyn, ...
SOCS-6 is a member of the suppressor of cytokine signaling (SOCS) family of proteins (SOCS-1 to SOCS-7 and CIS) which each contain a central SH2 domain and a carboxyl-terminal SOCS box. SOCS-1,...
Tarrant, Jacqueline M., Groom, Joanna, Metcalf, Donald, Li, Ruili, Borobokas, Bette, Wright, Mark D., ...
The tetraspanins are a family of integral membrane proteins with four transmembrane domains. These molecules form multimolecular networks on the surfaces of many different cell types. Gene-targeting...
Metcalf, Donald, Mifsud, Sandra, Di Rago, Ladina, Alexander, Warren S.
Injection of neonatal bone marrow cells from mice lacking the gene encoding suppressor of cytokine signaling 1 (SOCS1) into irradiated syngeneic 129/Sv or C57BL/6 mice led to a decreased survival,...
Hall, Mark A., Curtis, David J., Metcalf, Donald, Elefanty, Andrew G., Sourris, K., Robb, Lorraine, ...
Gene targeting studies have shown that the transcription factor SCL is critically important for embryonic hematopoiesis, but the early lethality of SCL null mice has precluded the genetic analysis of...
Metcalf, Donald, Di Rago, Ladina, Mifsud, Sandra
Mitogenic stimulation in vitro of mouse T lymphocytes induces the production of the hematopoietic cytokines granulocyte–macrophage colony-stimulating factor and IL-3. The present experiments showed...
Carpinelli, Marina R., Hilton, Douglas J., Metcalf, Donald, Antonchuk, Jennifer L., Hyland, Craig D., Mifsud, Sandra L., ...
Genetic screens in lower organisms, particularly those that identify modifiers of preexisting genetic defects, have been used successfully to order components of complex signaling pathways. To date,...
Brender, Christine, Columbus, Ruth, Metcalf, Donald, Handman, Emanuela, Starr, Robyn, Huntington, Nick, ...
Suppressors of cytokine signaling (SOCSs) are key regulators of cytokine-induced responses in hematopoietic as well as nonhematopoietic cells. SOCS1 and SOCS3 have been shown to modulate T-cell...
Critical role for granulocyte colony-stimulating factor in inflammatory arthritis
Lawlor, Kate E., Campbell, Ian K., Metcalf, Donald, O'Donnell, Kristy, Van Nieuwenhuijze, Annemarie, Roberts, Andrew W., ...
Granulocyte colony-stimulating factor (G-CSF) is a well known regulator of granulopoiesis, but the role of endogenous G-CSF in inflammatory joint disease has not been explored. We studied the...
Development of hydrocephalus in mice lacking SOCS7
Krebs, Danielle L., Metcalf, Donald, Merson, Tobias D., Voss, Anne K., Thomas, Tim, Zhang, Jian-Guo, ...
SOCS7 is a member of the suppressor of cytokine signaling (SOCS) family of proteins (SOCS1-SOCS7 and CIS). SOCS proteins are composed of an N-terminal domain of variable length, a central Src...
SOCS2 negatively regulates growth hormone action in vitro and in vivo
Greenhalgh, Christopher J., Rico-Bautista, Elizabeth, Lorentzon, Mattias, Thaus, Anne L., Morgan, Phillip O., Willson, Tracy A., ...
Mice deficient in SOCS2 display an excessive growth phenotype characterized by a 30–50% increase in mature body size. Here we show that the SOCS2–/– phenotype is dependent upon the presence of...
Nicholson, Sandra E., Metcalf, Donald, Sprigg, Naomi S., Columbus, Ruth, Walker, Francesca, Silva, Anabel, ...
The suppressors of cytokine signaling (SOCS) proteins are a family of SH2 domain-containing intracellular inhibitors of cytokine signal transduction that act by several different mechanisms. Recent...
Burgess, Antony W., Metcalf, Donald, Russell, Sue H. M., Nicola, Nicos A.
The formation of mature haemopoietic cells is controlled by hormones that specifically stimulate the progenitor cells of the granulocyte/macrophage, eosinophil, megakaryocyte and erythroid pathways....
Robb, Lorraine, Boyle, Kristy, Rakar, Steven, Hartley, Lynne, Lochland, Janelle, Roberts, Andrew W., ...
The suppressor of cytokine-signaling (SOCS) proteins act as negative-feedback inhibitors of cytokine and growth-factor-induced signal transduction. In vivo studies have implicated SOCS3 as a negative...
Inactivation of PU.1 in adult mice leads to the development of myeloid leukemia
Metcalf, Donald, Dakic, Aleksandar, Mifsud, Sandra, Di Rago, Ladina, Wu, Li, Nutt, Stephen
Genetically primed adult C57BL mice were deleted of exon 5 of the gene encoding the transcription factor PU.1 by IFN activation of Cre recombinase. After a 13-week delay, conditionally deleted...
Thomas, Tim, Corcoran, Lynn M., Gugasyan, Raffi, Dixon, Mathew P., Brodnicki, Thomas, Nutt, Stephen L., ...
Monocytic leukemia zinc finger protein (MOZ), a transcriptional coactivator and member of the MYST family of histone acetyltransferases, is the target of recurrent translocations in acute myeloid...
Metcalf, Donald, Di Rago, Ladina, Mifsud, Sandra, Hartley, Lynne, Alexander, Warren S.
Mice lacking the gene encoding the suppressor of cytokine signaling-1 (SOCS-1 −/−) and heterozygous for the IFN–γ gene (IFN-γ +/−) avoided the IFN-γ-dependent preweaning death of SOCS-1...
Twenty proteins containing a C-terminal SOCS box form five structural classes
Hilton, Douglas J., Richardson, Rachael T., Alexander, Warren S., Viney, Elizabeth M., Willson, Tracy A., Sprigg, Naomi S., ...
The four members of the recently identified suppressor of cytokines signaling family (SOCS-1, SOCS-2, SOCS-3, and CIS, where CIS is cytokine-inducible SH2-containing protein) appear, by various...
Grossmann, Mathis, Metcalf, Donald, Merryfull, Julie, Beg, Amer, Baltimore, David, Gerondakis, Steve
Individual Rel/NF-κB transcription factors, although dispensable for the development and maturation of most hemopoietic cells, are critical regulators of normal immune function. Redundancy among...
Nicholson, Sandra E., De Souza, David, Fabri, Louis J., Corbin, Jason, Willson, Tracy A., Zhang, Jian-Guo, ...
Suppressor of cytokine signaling-3 (SOCS-3) is one member of a family of intracellular inhibitors of signaling pathways initiated by cytokines that use, among others, the common receptor subunit...
Hematopoietic stem cell deficiencies in mice lacking c-Mpl, the receptor for thrombopoietin
Kimura, Shinya, Roberts, Andrew W., Metcalf, Donald, Alexander, Warren S.
Thrombopoietin (TPO) acts as a lineage-specific late-acting factor to stimulate megakaryocyte and platelet formation. However, analysis of mice lacking either the cytokine or its receptor, c-Mpl,...
Elefanty, Andrew G., Begley, C. Glenn, Metcalf, Donald, Barnett, Louise, Köntgen, Frank, Robb, Lorraine
Gene targeting experiments have demonstrated that the transcription factor SCL is essential for primitive and definitive hematopoiesis in the mouse. To study the functional properties of...
Apoptosis regulator Bcl-w is essential for spermatogenesis but appears otherwise redundant
Print, Cristin G., Loveland, Kate L., Gibson, Leonie, Meehan, Terri, Stylianou, Anna, Wreford, Nigel, ...
Proteins of the Bcl-2 family are important regulators of apoptosis in many tissues of the embryo and adult. The recently isolated bcl-w gene encodes a pro-survival member of the Bcl-2 family, which...
The stimulation by Flk2-ligand (FL) of blast colony formation by murine bone marrow cells was selectively potentiated by the addition of regulators sharing in common the gp130 signaling...
Liver degeneration and lymphoid deficiencies in mice lacking suppressor of cytokine signaling-1
Starr, Robyn, Metcalf, Donald, Elefanty, Andrew G., Brysha, Marta, Willson, Tracy A., Nicola, Nicos A., ...
SOCS-1, a member of the suppressor of cytokine signaling (SOCS) family, was identified in a genetic screen for inhibitors of interleukin 6 signal transduction. SOCS-1 transcription is induced by...
Ogilvy, Sarah, Metcalf, Donald, Print, Cristin G., Bath, Mary L., Harris, Alan W., Adams, Jerry M.
Bcl-2, which can both reduce apoptosis and retard cell cycle entry, is thought to have important roles in hematopoiesis. To evaluate the impact of its ubiquitous overexpression within this system, we...
Leptin can induce proliferation, differentiation, and functional activation of hemopoietic cells
Gainsford, Timothy, Willson, Tracy A., Metcalf, Donald, Handman, Emanuela, McFarlane, Clare, Ng, Ashley, ...
Many cytokines exert their biological effect through members of the hemopoietin receptor family. Using degenerate oligonucleotides to the common WSXWS motif, we have cloned from human hemopoietic...
Zhang, Jian-Guo, Farley, Alison, Nicholson, Sandra E., Willson, Tracy A., Zugaro, Lisa M., Simpson, Richard J., ...
The suppressors of cytokine signaling (SOCS) family of proteins act as intracellular inhibitors of several cytokine signal transduction pathways. Their expression is induced by cytokine activation of...
Normal mouse marrow cells were stimulated by stem cell factor (SCF) to form dispersed or multicentric blast colonies containing progenitor cells committed to various hematopoietic lineages....
Placental defects and embryonic lethality in mice lacking suppressor of cytokine signaling 3
Roberts, Andrew W., Robb, Lorraine, Rakar, Steven, Hartley, Lynne, Cluse, Leonie, Nicola, Nicos A., ...
Mice lacking suppressor of cytokine signaling 3 (SOCS3) exhibited embryonic lethality with death occurring between days 11 and 13 of gestation. At this stage, SOCS3−/− embryos were slightly...
Zhang, Jian-Guo, Metcalf, Donald, Rakar, Steven, Asimakis, Maria, Greenhalgh, Christopher J., Willson, Tracy A., ...
Suppressor of Cytokine Signaling-1 (SOCS-1) is an essential physiological inhibitor of IFN-γ signaling. Mice lacking this gene die in the early postnatal period from a disease characterized by...
Functional Analysis of Asb-1 Using Genetic Modification in Mice
Kile, Benjamin T., Metcalf, Donald, Mifsud, Sandra, DiRago, Ladina, Nicola, Nicos A., Hilton, Douglas J., ...
The Asbs are a family of ankyrin repeat proteins that, along with four other protein families, contain a C-terminal SOCS box motif, which was first identified in the suppressor of cytokine signaling...
Metcalf, Donald, Mifsud, Sandra, Di Rago, Ladina, Nicola, Nicos A., Hilton, Douglas J., Alexander, Warren S.
Mice with inactivation of the gene encoding the suppressor of cytokine signaling-1 (SOCS-1) die in neonatal life with an IFN-γ-dependent inflammatory disease dominated by fatty degeneration and...
Pohl, Thomas, Gugasyan, Raffi, Grumont, Raelene J., Strasser, Andreas, Metcalf, Donald, Tarlinton, David, ...
Transcription factors NF-κB1 and c-Rel, individually dispensable during embryogenesis, serve similar, yet distinct, roles in the function of mature hemopoietic cells. Redundancy among Rel/NF-κB...
Krebs, Danielle L., Uren, Rachel T., Metcalf, Donald, Rakar, Steven, Zhang, Jian-Guo, Starr, Robyn, ...
SOCS-6 is a member of the suppressor of cytokine signaling (SOCS) family of proteins (SOCS-1 to SOCS-7 and CIS) which each contain a central SH2 domain and a carboxyl-terminal SOCS box. SOCS-1,...
Tarrant, Jacqueline M., Groom, Joanna, Metcalf, Donald, Li, Ruili, Borobokas, Bette, Wright, Mark D., ...
The tetraspanins are a family of integral membrane proteins with four transmembrane domains. These molecules form multimolecular networks on the surfaces of many different cell types. Gene-targeting...
Metcalf, Donald, Mifsud, Sandra, Di Rago, Ladina, Alexander, Warren S.
Injection of neonatal bone marrow cells from mice lacking the gene encoding suppressor of cytokine signaling 1 (SOCS1) into irradiated syngeneic 129/Sv or C57BL/6 mice led to a decreased survival,...