Flibotte, Stephane, Moerman, Donald G
Abstract Background Microarray comparative genomic hybridization (CGH) is currently one of the most powerful techniques to measure DNA copy number in large genomes. In humans, microarray CGH is...
Transcriptome analysis for Caenorhabditis elegansbased on novel expressed sequence tags (2008)
Shin, Heesun, Hirst, Martin, Bainbridge, Matthew N, Magrini, Vincent, Mardis, Elaine, Moerman, Donald G, ...
Abstract Background We have applied a high-throughput pyrosequencing technology for transcriptome profiling of Caenorhabditis elegans in its first larval stage. Using this approach, we have generated...
Jones, Martin R, Maydan, Jason S, Flibotte, Stephane, Moerman, Donald G, Baillie, David L
Abstract Background: A collection of genetic deficiencies covering over 70% of the Caenorhabditis elegans genome exists, however the application of these valuable biological tools has been limited...
High-Throughput In Vivo Analysis of Gene Expression in Caenorhabditis elegans (2007)
Rebecca Hunt-Newbury, Ryan Viveiros, Robert Johnsen, Allan Mah, Dina Anastas, Lily Fang, ...
Using DNA sequences 5′ to open reading frames, we have constructed green fluorescent protein (GFP) fusions and generated spatial and temporal tissue expression profiles for 1,886 specific genes in...
Establishment of a tissue-specific RNAi system in C. elegans (2007)
Qadota, Hiroshi, Inoue, Makiko, Hikita, Takao, Köppen, Mathias, Hardin, Jeffrey D., Amano, Mutsuki, ...
In C. elegans, mosaic analysis is a powerful genetic tool for determining in which tissue or specific cells a gene of interest is required. For traditional mosaic analysis, a loss-of-function mutant...
Chen, Nansheng, Mah, Allan, Blacque, Oliver E, Chu, Jeffrey, Phgora, Kiran, Bakhoum, Mathieu W, ...
Abstract Background The recent availability of genome sequences of multiple related Caenorhabditis species has made it possible to identify, using comparative genomics, similarly transcribed genes in...
Mullen, Gregory P., Rogalski, Teresa M., Bush, Jason A., Gorji, Poupak Rahmani, Moerman, Donald G.
The unc-52 gene encodes the nematode homologue of mammalian perlecan, the major heparan sulfate proteoglycan of the extracellular matrix. This is a large complex protein with regions similar to...
Mercer, Kristina B., Flaherty, Denise B., Miller, Rachel K., Qadota, Hiroshi, Tinley, Tina L., Moerman, Donald G., ...
To further understand the assembly and maintenance of the muscle contractile apparatus, we have identified a new protein, UNC-98, in the muscle of Caenorhabditis elegans. unc-98 mutants display...
Identification of a nematode chemosensory gene family
Chen, Nansheng, Pai, Shraddha, Zhao, Zhongying, Mah, Allan, Newbury, Rebecca, Johnsen, Robert C., ...
Taking advantage of the recent availability of the whole genome sequence of Caenorhabditis briggsae, a closely related nematode to Caenorhabditis elegans, we have examined the chemosensory gene...
Essential Genes and Deficiencies in the UNC-22 IV Region of CAENORHABDITIS ELEGANS
Rogalski, Teresa M., Moerman, Donald G., Baillie, David L.
Five formaldehyde-induced deficiencies that uncover unc-22 IV, a gene affecting muscle structure in the nematode Caenorhabditis elegans were isolated and positioned. The largest deficiency, sDf2,...
Rogalski, Teresa M, Gilbert, Mary M, Devenport, Danelle, Norman, Kenneth R, Moerman, Donald G
The UNC-112 protein is required during initial muscle assembly in C. elegans to form dense bodies and M-lines. Loss of this protein results in arrest at the twofold stage of embryogenesis. In...
Mullen, Gregory P., Rogalski, Teresa M., Bush, Jason A., Gorji, Poupak Rahmani, Moerman, Donald G.
The unc-52 gene encodes the nematode homologue of mammalian perlecan, the major heparan sulfate proteoglycan of the extracellular matrix. This is a large complex protein with regions similar to...
Mercer, Kristina B., Flaherty, Denise B., Miller, Rachel K., Qadota, Hiroshi, Tinley, Tina L., Moerman, Donald G., ...
To further understand the assembly and maintenance of the muscle contractile apparatus, we have identified a new protein, UNC-98, in the muscle of Caenorhabditis elegans. unc-98 mutants display...
Identification of a nematode chemosensory gene family
Chen, Nansheng, Pai, Shraddha, Zhao, Zhongying, Mah, Allan, Newbury, Rebecca, Johnsen, Robert C., ...
Taking advantage of the recent availability of the whole genome sequence of Caenorhabditis briggsae, a closely related nematode to Caenorhabditis elegans, we have examined the chemosensory gene...
Essential Genes and Deficiencies in the UNC-22 IV Region of CAENORHABDITIS ELEGANS
Rogalski, Teresa M., Moerman, Donald G., Baillie, David L.
Five formaldehyde-induced deficiencies that uncover unc-22 IV, a gene affecting muscle structure in the nematode Caenorhabditis elegans were isolated and positioned. The largest deficiency, sDf2,...
Rogalski, Teresa M, Gilbert, Mary M, Devenport, Danelle, Norman, Kenneth R, Moerman, Donald G
The UNC-112 protein is required during initial muscle assembly in C. elegans to form dense bodies and M-lines. Loss of this protein results in arrest at the twofold stage of embryogenesis. In...
Chen, Nansheng, Mah, Allan, Blacque, Oliver E, Chu, Jeffrey, Phgora, Kiran, Bakhoum, Mathieu W, ...
Comparative genomic analysis of three nematode species identifies 93 genes that encode putative components of the ciliated neurons in C. elegans and are subject to the same regulatory control.
Maydan, Jason S., Flibotte, Stephane, Edgley, Mark L., Lau, Joanne, Selzer, Rebecca R., Richmond, Todd A., ...
We have developed array Comparative Genomic Hybridization for Caenorhabditis elegans as a means of screening for novel induced deletions in this organism. We designed three microarrays consisting of...
High-Throughput In Vivo Analysis of Gene Expression in Caenorhabditis elegans
Hunt-Newbury, Rebecca, Viveiros, Ryan, Johnsen, Robert, Mah, Allan, Anastas, Dina, Fang, Lily, ...
Using DNA sequences 5′ to open reading frames, we have constructed green fluorescent protein (GFP) fusions and generated spatial and temporal tissue expression profiles for 1,886 specific genes in...
The molecular signature and cis-regulatory architecture of a C. elegans gustatory neuron
Etchberger, John F., Lorch, Adam, Sleumer, Monica C., Zapf, Richard, Jones, Steven J., Marra, Marco A., ...
Taste receptor cells constitute a highly specialized cell type that perceives and conveys specific sensory information to the brain. The detailed molecular composition of these cells and the...
Identification and analysis of internal promoters in Caenorhabditis elegans operons
Huang, Peiming, Pleasance, Erin D., Maydan, Jason S., Hunt-Newbury, Rebecca, O’Neil, Nigel J., Mah, Allan, ...
The current Caenorhabditis elegans genomic annotation has many genes organized in operons. Using directionally stitched promoter∷GFP methodology, we have conducted the largest survey to date on the...
Hobert, Oliver, Moerman, Donald G., Clark, Kathleen A., Beckerle, Mary C., Ruvkun, Gary
We describe here the molecular and functional characterization of the Caenorhabditis elegans unc-97 gene, whose gene product constitutes a novel component of muscular adherens junctions. UNC-97 and...
α spectrin is essential for morphogenesis and body wall muscle formation in Caenorhabditis elegans
Norman, Kenneth R., Moerman, Donald G.
Acommon feature of multicellular animals is the ubiquitous presence of the spectrin cytoskeleton. Although discovered over 30 yr ago, the function of spectrin in nonerythrocytes has remained elusive....
Rogalski, Teresa M., Mullen, Gregory P., Gilbert, Mary M., Williams, Benjamin D., Moerman, Donald G.
Embryos homozygous for mutations in the unc-52, pat-2, pat-3, and unc-112 genes of C. elegans exhibit a similar Pat phenotype. Myosin and actin are not organized into sarcomeres in the body wall...