Reduced reliance on the trace element selenium during evolution of mammals (2008)
Lobanov, Alexey V, Hatfield, Dolph L, Gladyshev, Vadim N
Abstract Background Selenium (Se) is an essential trace element that occurs in proteins in the form of selenocysteine (Sec). It is transported throughout the body in the form of Sec residues in...
Loss of housekeeping selenoprotein expression in mouse liver modulates lipoprotein metabolism (2008)
Sengupta, Aniruddha, Carlson, Bradley A., Hoffmann, Victoria J., Gladyshev, Vadim N., Hatfield, Dolph L.
Selenium is incorporated into proteins as selenocysteine (Sec), which is dependent on its specific tRNA, designated tRNA [Ser]Sec. Targeted removal of the tRNA [Ser]Sec gene (Trsp) in mouse...
Lobanov, Alexey V., Fomenko, Dmitri E., Zhang, Yan, Sengupta, Aniruddha, Hatfield, Dolph L., Gladyshev, Vadim N.
Background: Selenocysteine (Sec) is a selenium-containing amino acid that is co-translationally inserted into nascent polypeptides by recoding UGA codons. Selenoproteins occur in both eukaryotes and...
Xu, Xue-Ming, Carlson, Bradley A., Zhang, Yan, Mix, Heiko, Kryukov, Gregory V., Glass, Richard S., ...
We used comparative genomics and experimental analyses to show that (1) eukaryotes and archaea, which possess the selenocysteine (Sec) protein insertion machinery contain an enzyme,...
Lobanov, Alexey V, Fomenko, Dmitri E, Zhang, Yan, Sengupta, Aniruddha, Hatfield, Dolph L, Gladyshev, Vadim N
Abstract Background Selenocysteine (Sec) is a selenium-containing amino acid that is co-translationally inserted into nascent polypeptides by recoding UGA codons. Selenoproteins occur in both...
Carlson, Bradley A., Moustafa, Mohamed E., Sengupta, Aniruddha, Schweizer, Ulrich, Shrimali, Rajeev, Rao, Mahadev, ...
Novel mouse models were developed in which the hepatic selenoprotein population was targeted for removal by disrupting the selenocysteine (Sec) tRNA [Ser]Sec gene (trsp) and then selenoprotein...
Nosovelov, Sergey V., Lobanov, Alexey V., Hua, Deame, Kasaikina, Marina V., Hatfield, Dolph L., Gladyshev, Vadim
Selenoproteins are an elite group of proteins containing a rare amino acid, selenocysteine (Sec), encoded by the codon, UGA. In eukaryotes, incorporation of Sec requires a Sec insertion sequence...
Novoselov, Sergey V., Lobanov, Alexey V., Hua, Deame, Kasaikina, Marina V., Hatfield, Dolph L., Gladyshev, Vadim
Selenoproteins are an elite group of proteins containing a rare amino acid, selenocysteine (Sec), encoded by the codon, UGA. In eukaryotes, incorporation of Sec requires a Sec insertion sequence...
Selenoprotein H Is a Nucleolar Thioredoxin-like Protein with a Unique Expression Pattern (2007)
Novoselov, Sergey V., Kryukov, Gregory V., Xu, Xue-Ming, Carlson, Bradley A., Hatfield, Dolph L., Gladyshev, Vadim N.
The human selenoproteome consists of 25 known selenoproteins, but functions of many of these proteins are not known. Selenoprotein H (SelH) is a recently discovered 14-kDa mammalian protein with no...
Selenophosphate synthetase 2 is essential for selenoprotein biosynthesis (2007)
Xu, Xue-Ming, Carlson, Bradley A., Irons, Robert, Mix, Heiko, Zhong, Nianxin, Gladyshev, Vadim, ...
Selenophosphate synthetase (SelD) generates the selenium donor for selenocysteine biosynthesis in eubacteria. One homologue of SelD in eukaryotes is SPS1 (selenophosphate synthetase 1) and a second...
Biosynthesis of Selenocysteine on Its tRNA in Eukaryotes (2007)
Xu, Xue-Ming, Carlson, Bradley A., Mix, Heiko, Zhang, Yan, Saira, Kazima, Glass, Richard S., ...
Selenocysteine (Sec) is cotranslationally inserted into protein in response to UGA codons and is the 21st amino acid in the genetic code. However, the means by which Sec is synthesized in eukaryotes...
Is there a twenty third amino acid in the genetic code? (2006)
Lobanov, Alexey V., Kryukov, Gregory V., Hatfield, Dolph L., Gladyshev, Vadim
The universal genetic code includes 20 common amino acids. In addition, selenocysteine (Sec) and pyrrolysine (Pyl), known as the twenty first and twenty second amino acids, are encoded by UGA and...
"Preface" to Selenium: Its Molecular Biology and Role in Human Health, Second Edition (2006)
Hatfield, Dolph L., Berry, Marla J., Gladyshev, Vadim N.
The purpose of the new edition book is to inform the reader of many new discoveries and to examine our present knowledge of the molecular biology of selenium, its incorporation into proteins as...
Mouse models for assessing the role of selenoproteins in health and development (2006)
Carlson, Bradley A., Xu, Xue-Ming, Shrimali, Rajeev, Sengupta, Aniruddha, Yoo, Min-Hyuk, Zhong, Nianxin, ...
Mouse models have been generated to assess the roles of selenoproteins involved with housekeeping tasks and/or stress-related phenomena in development and health. Each mouse model has taken advantage...
Salinas, Gustavo, Romero, Héctor, Xu, Xue-Ming, Carlson, Bradley A., Hatfield, Dolph L., Gladyshev, Vadim N.
The complete sequencing of genomes and the development of in silico methods for identification of genes encoding selenocysteine (Sec)-containing proteins have greatly contributed to shape our view on...
The 15-kDa selenoprotein (Sep15): functional analysis and role in cancer (2006)
Labunskyy, Vyacheslav M., Gladyshev, Vadim N., Hatfield, Dolph L.
The 15-kDa selenoprotein (Sep15) was identified several years ago as a protein of unknown function. In recent years, several lines of evidence implicated Sepl5 in the effect of dietary selenium in...
Mammalian and other eukaryotic selenocysteine tRNAs (2006)
Carlson, Bradley A., Xu, Xue-Ming, Shrimali, Rajeev, Sengupta, Aniruddha, Yoo, Min-Hyuk, Irons, Robert, ...
Selenocysteine (Sec) tRNA occupies a prominent position in the expression of selenoproteins as it is essential for their synthesis and it provides the means by which selenium is co-translationally...
Yoo, Min-Hyuk, Xu, Xue-Ming, Carlson, Bradley A., Gladyshev, Vadim N., Hatfield, Dolph L.
Dietary selenium has potent cancer prevention activity. Both low molecular weight selenocompounds and selenoproteins are implicated in this effect. Thioredoxin reductase 1 (TR1) is one of the major...
The Plasmodium selenoproteome (2006)
Lobanov, Alexey V., Delgado, Cesar, Rahlfs, Stefan, Novoselov, Sergey V., Kryukov, Gregory V., Gromer, Stephan, ...
The use of selenocysteine (Sec) as the 21st amino acid in the genetic code has been described in all three major domains of life. However, within eukaryotes, selenoproteins are only known in animals...
Xu, Xue-Ming, Mix, Heiko, Carlson, Bradley A., Grabowski, Paula J., Gladyshev, Vadim N., Berry, Marla J., ...
Selenocysteine (Sec) is inserted into selenoproteins co-translationally with the help of various cis- and trans-acting factors. The specific mechanisms of Sec biosynthesis and insertion into protein...
Labunskyy, Vyacheslav M., Ferguson, Andrew D., Fomenko, Dmitri E., Chelliah, Yogarany, Hatfield, Dolph L., Gladyshev, Vadim N.
Selenium is an essential trace element with potent cancer prevention activity in mammals. The 15-kDa selenoprotein (Sep15) has been implicated in the chemopreventive effect of dietary selenium....
Mammalian Selenoprotein Thioredoxin-glutathione Reductase (2005)
Su, Dan, Novoselov, Sergey V., Sun, Qi-An, Moustafa, Mohamed E., Zhou, You, Oko, Richard, ...
Thioredoxin reductases (TRs) are important redox regulatory enzymes, which control the redox state of thioredoxins. Mammals have cytosolic and mitochondrial TRs, which contain an essential...
Carlson, Bradley A., Xu, Xue-Ming, Gladyshev, Vadim N., Hatfield, Dolph L.
Selenium is an essential micronutrient in the diet of mammals and has many health benefits. Selenium-containing proteins are responsible for most, if not all, of these benefits. This element is...
Carlson, Bradley A., Xu, Xue-Ming, Gladyshev, Vadim N., Hatfield, Dolph L.
Selenocysteine (Sec) is the 21st amino acid in the genetic code. Its tRNA is variably methylated on the 2'-Ohydroxyl site of the ribosyl moiety at position 34 (Um34). Herein, we identified a role of...
Identification and characterization of phosphoseryl-tRNA [Ser]Sec kinase (2004)
Carlson, Bradley A., Xu, Xue-Ming, Kryukov, Gregory V., Rao, Mahadev, Berry, Marla J., Gladyshev, Vadim N., ...
In 1970, a kinase activity that phosphorylated a minor species of seryl-tRNA to form phosphoseryl-tRNA was found in rooster liver [Maenpaa, P. H. & Bernfield, M. R. (1970) Proc. Natl. Acad. Sci. USA...
Carlson, Bradley A., Novoselov, Sergey V., Kumaraswamy, Easwari, Jae Lee, Byeong, Anver, Miriam R., Gladyshev, Vadim N., ...
Selenium is essential in mammalian embryonic development. However, in adults, selenoprotein levels in several organs including liver can be substantially reduced by selenium deficiency without any...
Kwon, So Yeon, Badenhorst, Paul, Martin-Romero, F. Javier, Carlson, Bradley A., Paterson, Bruce M., Gladyshev, Vadim, ...
Selenium is implicated in many diseases, including cancer, but its function at the molecular level is poorly understood. BthD is one of three selenoproteins recently identified in Drosophila. To...
Chlamydomonas reinhardtii selenocysteine tRNA [Ser]Sec (2003)
Rao, Mahadev, Carlson, Bradley A., Novoselov, Sergey V., Weeks, Donald P., Gladyshev, Vadim, Hatfield, Dolph L.
Eukaryotic selenocysteine (Sec) protein insertion machinery was thought to be restricted to animals, but the occurrence of both Sec-containing proteins and the Sec insertion system was recently found...
Selective Removal of the Selenocysteine tRNA [Ser]Sec Gene (Trsp) in Mouse Mammary Epithelium (2003)
Kumaraswamy, Easwari, Carlson, Bradley A., Morgan, Fanta, Miyoshi, Keiko, Robinson, Gertraud W., Su, Dan, ...
Mice homozygous for an allele encoding the selenocysteine (Sec) tRNA [Ser]Sec gene (Trsp) flanked by loxP sites were generated. Cre recombinase-dependent removal of Trsp in these mice was lethal to...
Minireview: How Selenium Has Altered Our Understanding of the Genetic Code (2002)
Hatfield, Dolph L., Gladyshev, Vadim
Selenium is an essential micronutrient in the diet of many life forms, including humans and other mammals. Significant health benefits have been attributed to this element. It is rapidly becoming...
Korotkov, Konstantin V., Novoselov, Sergey V., Hatfield, Dolph L., Gladyshev, Vadim
Selenocysteine (Sec), the 21st amino acid in protein, is encoded by UGA. The Sec insertion sequence (SECIS) element, which is the stem-loop structure present in 3' untranslated regions (UTRs) of...
Selenium Metabolism in Drosophila (2001)
Martin-Romero, F. Javier, Kryukov, Gregory V., Lobanov, Alexei V., Carlson, Bradley A., Lee, Byeong Jae, Gladyshev, Vadim N., ...
Selenocysteine is a rare amino acid in protein that is encoded by UGA with the requirement of a downstream mRNA stem-loop structure, the selenocysteine insertion sequence element. To detect...
Gladyshev, Vadim, Diamond, Alan M., Hatfield, Dolph L.
The 15 kDa selenoprotein (Sepl5) is one of several recently identified selenoproteins. It contains a single selenocysteine residue in the middle of a 162-amino acid open reading frame and has no...
Korotkov, Konstantin V., Kumaraswamy, Easwari, Zhou, You, Hatfield, Dolph L., Gladyshev, Vadim N.
Mammalian selenocysteine-containing proteins characterized with respect to function are involved in redox processes and exhibit distinct expression patterns and cellular locations. A recently...
Heterogeneity within Animal Thioredoxin Reductases (2001)
Sun, Qi-An, Zappacosta, Francesca, Factor, Valentina M., Wirth, Peter J., Hatfield, Dolph L., Gladyshev, Vadim N.
Animal thioredoxin reductases (TRs) are selenocysteine- containing flavoenzymes that utilize NADPH for reduction of thioredoxins and other protein and nonprotein substrates. Three types of mammalian...
Structure-Expression Relationships of the 15-kDa Selenoprotein Gene (2000)
Kumaraswamy, Easwari, Malyhk, Andrey, Korotkov, Konstantin V., Kozyavkin, Sergei, Hu, Yajun, Lee, Byeong J., ...
Selenium has been implicated in cancer prevention, but the mechanism and possible involvement of selenoproteins in this process are not understood. To elucidate whether the 15-kDa selenoprotein may...
Redox Regulation of Cell Signaling by Selenocysteine in Mammalian Thioredoxin Reductases (1999)
Sun, Qi-An, Wu, Yalin, Zappacosta, Francesca, Jeang, Kuan-Teh, Jae Lee, Byeong, Hatfield, Dolph L., ...
The intracellular generation of reactive oxygen species, together with the thioredoxin and glutathione systems, is thought to participate in redox signaling in mammalian cells. The activity of...
Gladyshev, Vadim, Stadtman, Thressa C., Hatfield, Dolph L., Jeang, Kuan-Teh
It has been observed previously that plasma selenium and glutathione levels are subnormal in HIV-infected individuals, and plasma glutathione peroxidase activity is decreased. Under these conditions...
Selenocysteine-Containing Proteins in Mammals (1998)
Gladyshev, Vadim N., Hatfield, Dolph L.
Since the recent discovery of selenocysteine as the 21st amino acid in protein, the field of selenium biology has rapidly expanded. Twelve mammalian selenoproteins have been characterized to date and...
A New Human Selenium-containing Protein (1998)
Gladyshev, Vadim N., Jeang, Kuan Teh, Wootton, John C., Hatfield, Dolph L.
Selenium which occurs in proteins as the amino acid, selenocysteine, is essential for numerous biological processes and for human health. A prominent 75Se-labeled protein detected in human T-cells...
Gladyshev, Vadim N., Stadtman, Thressa C., Hatfield, Dolph L., Jeang, Kuan-Teh
It has been observed previously that plasma selenium and glutathione levels are subnormal in HIV-infected individuals, and plasma glutathione peroxidase activity is decreased. Under these conditions...
Moustafa, Mohamed E., Carlson, Bradley A., El-Saadani, Muhammad A., Kryukov, Gregory V., Sun, Qi-An, Harney, John W., ...
Selenocysteine (Sec) tRNA (tRNA[Ser]Sec) serves as both the site of Sec biosynthesis and the adapter molecule for donation of this amino acid to protein. The consequences on selenoprotein...
Novoselov, Sergey V., Rao, Mahadev, Onoshko, Natalia V., Zhi, Huijun, Kryukov, Gregory V., Xiang, Youbin, ...
Known eukaryotic selenocysteine (Sec)-containing proteins are animal proteins, whereas selenoproteins have not been found in yeast and plants. Surprisingly, we detected selenoproteins in a member of...
Korotkov, Konstantin V., Novoselov, Sergey V., Hatfield, Dolph L., Gladyshev, Vadim N.
Selenocysteine (Sec), the 21st amino acid in protein, is encoded by UGA. The Sec insertion sequence (SECIS) element, which is the stem-loop structure present in 3" untranslated regions (UTRs) of...
Selective Removal of the Selenocysteine tRNA[Ser]Sec Gene (Trsp) in Mouse Mammary Epithelium
Kumaraswamy, Easwari, Carlson, Bradley A., Morgan, Fanta, Miyoshi, Keiko, Robinson, Gertraud W., Su, Dan, ...
Mice homozygous for an allele encoding the selenocysteine (Sec) tRNA[Ser]Sec gene (Trsp) flanked by loxP sites were generated. Cre recombinase-dependent removal of Trsp in these mice was lethal to...
Kwon, So Yeon, Badenhorst, Paul, Martin-Romero, F. Javier, Carlson, Bradley A., Paterson, Bruce M., Gladyshev, Vadim N., ...
Selenium is implicated in many diseases, including cancer, but its function at the molecular level is poorly understood. BthD is one of three selenoproteins recently identified in Drosophila. To...
A novel RNA binding protein, SBP2, is required for the translation of mammalian selenoprotein mRNAs
Copeland, Paul R., Fletcher, Julia E., Carlson, Bradley A., Hatfield, Dolph L., Driscoll, Donna M.
In eukaryotes, the decoding of the UGA codon as selenocysteine (Sec) requires a Sec insertion sequence (SECIS) element in the 3′ untranslated region of the mRNA. We purified a SECIS binding...
Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase
Carlson, Bradley A., Xu, Xue-Ming, Kryukov, Gregory V., Rao, Mahadev, Berry, Marla J., Gladyshev, Vadim N., ...
In 1970, a kinase activity that phosphorylated a minor species of seryl-tRNA to form phosphoseryl-tRNA was found in rooster liver [Maenpaa, P. H. & Bernfield, M. R. (1970) Proc. Natl. Acad. Sci. USA...
Decoding apparatus for eukaryotic selenocysteine insertion
Tujebajeva, Rosa M., Copeland, Paul R., Xu, Xue-Ming, Carlson, Bradley A., Harney, John W., Driscoll, Donna M., ...
Decoding UGA as selenocysteine requires a unique tRNA, a specialized elongation factor, and specific secondary structures in the mRNA, termed SECIS elements. Eukaryotic SECIS elements are found in...
Lobanov, Alexey V., Delgado, Cesar, Rahlfs, Stefan, Novoselov, Sergey V., Kryukov, Gregory V., Gromer, Stephan, ...
The use of selenocysteine (Sec) as the 21st amino acid in the genetic code has been described in all three major domains of life. However, within eukaryotes, selenoproteins are only known in animals...
Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec
RAO, MAHADEV, CARLSON, BRADLEY A., NOVOSELOV, SERGEY V., WEEKS, DONALD P., GLADYSHEV, VADIM N., HATFIELD, DOLPH L.
Eukaryotic selenocysteine (Sec) protein insertion machinery was thought to be restricted to animals, but the occurrence of both Sec-containing proteins and the Sec insertion system was recently found...
Supramolecular Complexes Mediate Selenocysteine Incorporation In Vivo
Small-Howard, Andrea, Morozova, Nadya, Stoytcheva, Zoia, Forry, Erin P., Mansell, John B., Harney, John W., ...
Selenocysteine incorporation in eukaryotes occurs cotranslationally at UGA codons via the interactions of RNA-protein complexes, one comprised of selenocysteyl (Sec)-tRNA[Ser]Sec and its specific...
Gladyshev, Vadim N., Stadtman, Thressa C., Hatfield, Dolph L., Jeang, Kuan-Teh
It has been observed previously that plasma selenium and glutathione levels are subnormal in HIV-infected individuals, and plasma glutathione peroxidase activity is decreased. Under these conditions...
Moustafa, Mohamed E., Carlson, Bradley A., El-Saadani, Muhammad A., Kryukov, Gregory V., Sun, Qi-An, Harney, John W., ...
Selenocysteine (Sec) tRNA (tRNA[Ser]Sec) serves as both the site of Sec biosynthesis and the adapter molecule for donation of this amino acid to protein. The consequences on selenoprotein...
Novoselov, Sergey V., Rao, Mahadev, Onoshko, Natalia V., Zhi, Huijun, Kryukov, Gregory V., Xiang, Youbin, ...
Known eukaryotic selenocysteine (Sec)-containing proteins are animal proteins, whereas selenoproteins have not been found in yeast and plants. Surprisingly, we detected selenoproteins in a member of...
Korotkov, Konstantin V., Novoselov, Sergey V., Hatfield, Dolph L., Gladyshev, Vadim N.
Selenocysteine (Sec), the 21st amino acid in protein, is encoded by UGA. The Sec insertion sequence (SECIS) element, which is the stem-loop structure present in 3" untranslated regions (UTRs) of...
Selective Removal of the Selenocysteine tRNA[Ser]Sec Gene (Trsp) in Mouse Mammary Epithelium
Kumaraswamy, Easwari, Carlson, Bradley A., Morgan, Fanta, Miyoshi, Keiko, Robinson, Gertraud W., Su, Dan, ...
Mice homozygous for an allele encoding the selenocysteine (Sec) tRNA[Ser]Sec gene (Trsp) flanked by loxP sites were generated. Cre recombinase-dependent removal of Trsp in these mice was lethal to...
Kwon, So Yeon, Badenhorst, Paul, Martin-Romero, F. Javier, Carlson, Bradley A., Paterson, Bruce M., Gladyshev, Vadim N., ...
Selenium is implicated in many diseases, including cancer, but its function at the molecular level is poorly understood. BthD is one of three selenoproteins recently identified in Drosophila. To...
A novel RNA binding protein, SBP2, is required for the translation of mammalian selenoprotein mRNAs
Copeland, Paul R., Fletcher, Julia E., Carlson, Bradley A., Hatfield, Dolph L., Driscoll, Donna M.
In eukaryotes, the decoding of the UGA codon as selenocysteine (Sec) requires a Sec insertion sequence (SECIS) element in the 3′ untranslated region of the mRNA. We purified a SECIS binding...