Fujita−Becker, Setsuko, Dürrwang, Ulrike, Erent, Muriel, Clark, Richard, Geeves, Michael, Manstein, Dietmar J.
Molecular engineering of a backwards−moving myosin motor (2004)
Tsiavaliaris,Georgios, Fujita−Becker,Setsuko, Manstein,Dietmar J.
All members of the diverse myosin superfamily have a highly conserved globular motor domain that contains the actin− and nucleotide−binding sites and produces force and movement1, 2. The...
Molecular engineering of a backwards−moving myosin motor (2004)
Tsiavaliaris,Georgios, Fujita−Becker,Setsuko, Manstein,Dietmar J.
All members of the diverse myosin superfamily have a highly conserved globular motor domain that contains the actin− and nucleotide−binding sites and produces force and movement1, 2. The...
Molecular engineering of a backwards−moving myosin motor (2004)
Tsiavaliaris, Georgios, Fujita−Becker, Setsuko, Manstein, Dietmar J.
All members of the diverse myosin superfamily have a highly conserved globular motor domain that contains the actin− and nucleotide−binding sites and produces force and movement1, 2. The...
Molecular engineering of a backwards−moving myosin motor (2004)
Tsiavaliaris, Georgios, Fujita−Becker, Setsuko, Manstein, Dietmar J.
All members of the diverse myosin superfamily have a highly conserved globular motor domain that contains the actin− and nucleotide−binding sites and produces force and movement1, 2. The...
Interaction of Myosin Subfragment I with Forms of Monomeric Actin (2003)
Ballweber,Edda, Kiessling,Peter, Manstein,Dietmar J., Mannherz,Hans Georg
The ability of myosin SUBfragment 1 to interact with monomeric actin complexed to sequestering proteins was tested by a number of different techniques such as affinity absorption, chemical...
Interaction of Myosin Subfragment I with Forms of Monomeric Actin (2003)
Ballweber, Edda, Kiessling, Peter, Manstein, Dietmar J., Mannherz, Hans Georg
The ability of myosin SUBfragment 1 to interact with monomeric actin complexed to sequestering proteins was tested by a number of different techniques such as affinity absorption, chemical...
Tsiavaliaris,Georgios, Fujita-Becker,Setsuko, Batra,Renu, Levitsky,Dmitrii I., Kull,F. Jon, Geeves,Michael A., ...
Dominant-negative inhibition is a powerful genetic tool for the characterization of gene function in vivo, based on the specific impairment of a gene product by the coexpression of a mutant version...
PEVK Domain of Titin: An Entropic Spring with Actin-Binding Properties (2002)
Linke,Wolfgang A., Kulke,Michael, Li,Hongbin, Fujita-Becker,Setsuko, Neagoe,Ciprian, Manstein,Dietmar J., ...
The PEVKdomain of the giant muscle protein titin is a proline-rich sequence with unknown secondary/tertiary structure. Here we compared the force-extension behavior of cloned cardiac PEVK titin...
Crystal structure of the motor domain of a class-I myosin (2002)
Kollmar,Martin, Dürrwang,Ulrike, Kliche,Werner, Manstein,Dietmar J., Kull,F. Jon
The crystal structure of the motor domain of Dictyostelium discoideum myosin-IE, a monomeric unconventional myosin, was determined. The crystallographic asymmetric unit contains four independently...
Viscous force in cardiac myofibrils is caused by actin-PEVK- titin interaction (2002)
Kulke,Michael, Fujita-Becker,Setsuko, Neagoe,Ciprian, Manstein,Dietmar J., Gautel,Mathias, Linke,Wolfgang A.
PEVK Domain of Titin: An Entropic Spring with Actin-Binding Properties (2002)
Linke,Wolfgang A., Kulke,Michael, Li,Hongbin, Fujita-Becker,Setsuko, Neagoea,Ciprian, Manstein,Dietmar J., ...
The PEVKdomain of the giant muscle protein titin is a proline-rich sequence with unknown secondary/tertiary structure. Here we compared the force-extension behavior of cloned cardiac PEVK titin...
Toxoplasma gondii myosin A and its light chain: a fast, single-headed, plusend directed motor (2002)
Herm-Götz,Angelika, Weiss,Stefan, Stratmann,Rolf, Fujita-Becker,Setsuko, Ruff,Christine, Meyhöfer,Edgar, ...
Successful host cell invasion is a prerequisite for survival of the obligate intracellular apicomplexan parasites and establishment of infection. Toxoplasma gondii penetrates host cells by an active...
Klockow,Boris, Tichelaar,Willem, Madden,Dean R., Niemann,Hartmut H., Akiba,Toshihiko, Hirose,Keiko, ...
Here we show that Dictyostelium discoideum dynamin A is a fast GTPase, binds to negatively charged lipids, and self-assembles into rings and helices in a nucleotide-dependent manner, similar to human...
Toxoplasma gondii myosin A and its light chain: a fast, single-headed, plusend directed motor (2002)
Herm-Götz,Angelika, Weiss,Stefan, Stratmann,Rolf, Fujita-Becker,Setsuko, Ruff,Christine, Meyhöfer,Edgar, ...
Successful host cell invasion is a prerequisite for survival of the obligate intracellular apicomplexan parasites and establishment of infection. Toxoplasma gondii penetrates host cells by an active...
Viscous force in cardiac myofibrils is caused by actin-PEVK- titin interaction (2002)
Kulke, Michael, Fujita-Becker, Setsuko, Neagoe, Ciprian, Manstein, Dietmar J., Gautel, Mathias, Linke, Wolfgang A.
Toxoplasma gondii myosin A and its light chain: a fast, single-headed, plusend directed motor (2002)
Herm-Götz, Angelika, Weiss, Stefan, Stratmann, Rolf, Fujita-Becker, Setsuko, Ruff, Christine, Meyhöfer, Edgar, ...
Successful host cell invasion is a prerequisite for survival of the obligate intracellular apicomplexan parasites and establishment of infection. Toxoplasma gondii penetrates host cells by an active...
Klockow, Boris, Tichelaar, Willem, Madden, Dean R., Niemann, Hartmut H., Akiba, Toshihiko, Hirose, Keiko, ...
Here we show that Dictyostelium discoideum dynamin A is a fast GTPase, binds to negatively charged lipids, and self-assembles into rings and helices in a nucleotide-dependent manner, similar to human...
Crystal structure of the motor domain of a class-I myosin (2002)
Kollmar, Martin, Dürrwang, Ulrike, Kliche, Werner, Manstein, Dietmar J., Kull, F. Jon
The crystal structure of the motor domain of Dictyostelium discoideum myosin-IE, a monomeric unconventional myosin, was determined. The crystallographic asymmetric unit contains four independently...
PEVK Domain of Titin: An Entropic Spring with Actin-Binding Properties (2002)
Linke, Wolfgang A., Kulke, Michael, Li, Hongbin, Fujita-Becker, Setsuko, Neagoe, Ciprian, Manstein, Dietmar J., ...
The PEVKdomain of the giant muscle protein titin is a proline-rich sequence with unknown secondary/tertiary structure. Here we compared the force-extension behavior of cloned cardiac PEVK titin...
Tsiavaliaris, Georgios, Fujita-Becker, Setsuko, Batra, Renu, Levitsky, Dmitrii I., Kull, F. Jon, Geeves, Michael A., ...
Dominant-negative inhibition is a powerful genetic tool for the characterization of gene function in vivo, based on the specific impairment of a gene product by the coexpression of a mutant version...
Toxoplasma gondii myosin A and its light chain: a fast, single-headed, plusend directed motor (2002)
Herm-Götz, Angelika, Weiss, Stefan, Stratmann, Rolf, Fujita-Becker, Setsuko, Ruff, Christine, Meyhöfer, Edgar, ...
Successful host cell invasion is a prerequisite for survival of the obligate intracellular apicomplexan parasites and establishment of infection. Toxoplasma gondii penetrates host cells by an active...
PEVK Domain of Titin: An Entropic Spring with Actin-Binding Properties (2002)
Linke, Wolfgang A., Kulke, Michael, Li, Hongbin, Fujita-Becker, Setsuko, Neagoea, Ciprian, Manstein, Dietmar J., ...
The PEVKdomain of the giant muscle protein titin is a proline-rich sequence with unknown secondary/tertiary structure. Here we compared the force-extension behavior of cloned cardiac PEVK titin...
Lighting up the cell surface with evanescent wave microscopy (2001)
Toomre,Derek, Manstein,Dietmar J.
Evanescent wave microscopy, also termed total internal reflection fluorescence microscopy (TIR-FM), has shed new light on important cellular processes taking place near the plasma membrane. For...
Single-molecule tracking of myosins with genetically engineered amplifier domains (2001)
Ruff,Christine, Furch,Marcus, Brenner,Bernhard, Manstein,Dietmar J., Meyhöfer,Edgar
We combined protein engineering and single molecule measurements to directly record the step size of a series of myosin constructs with shortened and elongated artificial neck domains. Our results...
Structure of a genetically engineered molecular motor (2001)
Kliche,Werner, Fujita-Becker,Setsuko, Kollmar,Martin, Manstein,Dietmar J., Kull,F. Jon
Molecular motors move unidirectionally along polymer tracks, producing movement and force in an ATP-dependent fashion. They achieve this by amplifying small conformational changes in the...
Knetsch,Menno L.W., Schäfers,Nicole, Horstmann,Heinz, Manstein,Dietmar J.
Dictyostelium discoideum DdRacGap1 (DRG) contains both Rho-GEF and Rho-GAP domains, a feature it shares with mammalian Bcr and Abr. To elucidate the physiological role of this multifunctional...
Crystal structure of a dynamin GTPase domain (2001)
Niemann,Hartmut H., Knetsch,Menno L. W., Scherer,Anna, Manstein,Dietmar J., Kull,F. Jon
Dynamins form a family of multidomain GTPases involved in endocytosis, vesicle trafficking and maintenance of mitochondrial morphology. In contrast to the classical switch GTPases, a force-generating...
Kulke,Michael, Fujita-Becker,Setsuko, Rostkova,E., Neagoe,C., Labeit,D., Manstein,Dietmar J., ...
The giant muscle protein titin contains a unique sequence, the PEVK domain, the elastic properties of which contribute to the mechanical behavior of relaxed cardiomyocytes. Here, human N2-Bcardiac...
Structure of a genetically engineered molecular motor (2001)
Kliche,Werner, Fujita-Becker,Setsuko, Kollmar,Martin, Manstein,Dietmar J., Kull,F. Jon
Molecular motors move unidirectionally along polymer tracks, producing movement and force in an ATP-dependent fashion. They achieve this by amplifying small conformational changes in the...
Knetsch,Menno L.W., Schäfers,Nicole, Horstmann,Heinz, Manstein,Dietmar J.
Dictyostelium discoideum DdRacGap1 (DRG) contains both Rho-GEF and Rho-GAP domains, a feature it shares with mammalian Bcr and Abr. To elucidate the physiological role of this multifunctional...
Crystal structure of a dynamin GTPase domain (2001)
Niemann,Hartmut H., Knetsch,Menno L. W., Scherer,Anna, Manstein,Dietmar J., Kull,F. Jon
Dynamins form a family of multidomain GTPases involved in endocytosis, vesicle trafficking and maintenance of mitochondrial morphology. In contrast to the classical switch GTPases, a force-generating...
Structure of a genetically engineered molecular motor (2001)
Kliche, Werner, Fujita-Becker, Setsuko, Kollmar, Martin, Manstein, Dietmar J., Kull, F. Jon
Molecular motors move unidirectionally along polymer tracks, producing movement and force in an ATP-dependent fashion. They achieve this by amplifying small conformational changes in the...
Knetsch, Menno L.W., Schäfers, Nicole, Horstmann, Heinz, Manstein, Dietmar J.
Dictyostelium discoideum DdRacGap1 (DRG) contains both Rho-GEF and Rho-GAP domains, a feature it shares with mammalian Bcr and Abr. To elucidate the physiological role of this multifunctional...
Crystal structure of a dynamin GTPase domain (2001)
Niemann, Hartmut H., Knetsch, Menno L. W., Scherer, Anna, Manstein, Dietmar J., Kull, F. Jon
Dynamins form a family of multidomain GTPases involved in endocytosis, vesicle trafficking and maintenance of mitochondrial morphology. In contrast to the classical switch GTPases, a force-generating...
Single-molecule tracking of myosins with genetically engineered amplifier domains (2001)
Ruff, Christine, Furch, Marcus, Brenner, Bernhard, Manstein, Dietmar J., Meyhöfer, Edgar
We combined protein engineering and single molecule measurements to directly record the step size of a series of myosin constructs with shortened and elongated artificial neck domains. Our results...
Lighting up the cell surface with evanescent wave microscopy (2001)
Toomre, Derek, Manstein, Dietmar J.
Evanescent wave microscopy, also termed total internal reflection fluorescence microscopy (TIR-FM), has shed new light on important cellular processes taking place near the plasma membrane. For...
Kulke, Michael, Fujita-Becker, Setsuko, Rostkova, E., Neagoe, C., Labeit, D., Manstein, Dietmar J., ...
The giant muscle protein titin contains a unique sequence, the PEVK domain, the elastic properties of which contribute to the mechanical behavior of relaxed cardiomyocytes. Here, human N2-Bcardiac...
Structure of a genetically engineered molecular motor (2001)
Kliche, Werner, Fujita-Becker, Setsuko, Kollmar, Martin, Manstein, Dietmar J., Kull, F. Jon
Molecular motors move unidirectionally along polymer tracks, producing movement and force in an ATP-dependent fashion. They achieve this by amplifying small conformational changes in the...
Knetsch, Menno L.W., Schäfers, Nicole, Horstmann, Heinz, Manstein, Dietmar J.
Dictyostelium discoideum DdRacGap1 (DRG) contains both Rho-GEF and Rho-GAP domains, a feature it shares with mammalian Bcr and Abr. To elucidate the physiological role of this multifunctional...
Crystal structure of a dynamin GTPase domain (2001)
Niemann, Hartmut H., Knetsch, Menno L. W., Scherer, Anna, Manstein, Dietmar J., Kull, F. Jon
Dynamins form a family of multidomain GTPases involved in endocytosis, vesicle trafficking and maintenance of mitochondrial morphology. In contrast to the classical switch GTPases, a force-generating...
Stabilization of the actomyosin complex by negative charges on myosin (2000)
Furch,Marcus, Remmel,Bettina, Geeves,Michael A., Manstein,Dietmar J.
Sequence comparisons of members of the myosin superfamily show a high degree of charge conservation in a surface exposed helix (Dictyostelium discoideum myosin II heavy chain residues S510 to K546)....
Ponomarev,Michael A., Furch,Marcus, Levitsky,Dmitrii I., Manstein,Dietmar J.
The thermal unfolding of Dictyostelium discoideum myosin head fragments with alterations in the actin-binding surface loop 2 was studied by differential scanning calorimetry. Lengthening of loop 2...
Stabilization of the actomyosin complex by negative charges on myosin (2000)
Furch, Marcus, Remmel, Bettina, Geeves, Michael A., Manstein, Dietmar J.
Sequence comparisons of members of the myosin superfamily show a high degree of charge conservation in a surface exposed helix (Dictyostelium discoideum myosin II heavy chain residues S510 to K546)....
Ponomarev, Michael A., Furch, Marcus, Levitsky, Dmitrii I., Manstein, Dietmar J.
The thermal unfolding of Dictyostelium discoideum myosin head fragments with alterations in the actin-binding surface loop 2 was studied by differential scanning calorimetry. Lengthening of loop 2...
Role of the salt-bridge between switch-1 and switch-2 of Dictyostelium myosin (1999)
Furch,Marcus, Fujita-Becker,Setsuko, Geeves,Michael A., Holmes,Kenneth C., Manstein,Dietmar J.
Motifs N2 and N3, also referred to as switch-1 and switch-2, form part of the active site of molecular motors such as myosins and kinesins. In the case of myosin, N3 is thought to act as a...
Batra,Renu, Manstein,Dietmar J.
We created a Dictyostelium discoideum myosin II mutant in which the highly conserved residue Trp-501 was replaced by a tyrosine residue. The mutant myosin alone, when expressed in a Dictyostelium...
Batra,Renu, Geeves,Michael A., Manstein,Dietmar J.
Three conserved glycine residues in the reactive thiol region of Dictyostelium discoideummyosin II were replaced by alanine residues. The resulting mutants G680A, G684A, and G691A were expressed in...
Functional characterization of the secondary actin binding site of myosin II (1999)
Van Dijk,J., Furch,Marcus, Lafont,C., Manstein,Dietmar J., Chaussepied,P.
Batra, Renu, Geeves, Michael A., Manstein, Dietmar J.
Three conserved glycine residues in the reactive thiol region of Dictyostelium discoideummyosin II were replaced by alanine residues. The resulting mutants G680A, G684A, and G691A were expressed in...
Batra, Renu, Manstein, Dietmar J.
We created a Dictyostelium discoideum myosin II mutant in which the highly conserved residue Trp-501 was replaced by a tyrosine residue. The mutant myosin alone, when expressed in a Dictyostelium...
Role of the salt-bridge between switch-1 and switch-2 of Dictyostelium myosin (1999)
Furch, Marcus, Fujita-Becker, Setsuko, Geeves, Michael A., Holmes, Kenneth C., Manstein, Dietmar J.
Motifs N2 and N3, also referred to as switch-1 and switch-2, form part of the active site of molecular motors such as myosins and kinesins. In the case of myosin, N3 is thought to act as a...
Functional characterization of the secondary actin binding site of myosin II (1999)
Van Dijk, J., Furch, Marcus, Lafont, C., Manstein, Dietmar J., Chaussepied, P.
Kinetic Characterization of myosin head fragments with long-lived myosin ATP states (1998)
Friedman,A. L., Geeves,Michael A., Manstein,Dietmar J., Spudich,J. A.
Furch,Marcus, Geeves,Michael A., Manstein,Dietmar J.
The effects of mutations in an actin-binding surface loop of myosin (loop 2) are described. Part of loop 2, the segment between myosin residues 618 and 622, was replaced with sequences enlarged by...
Levitsky,D. I., Ponomarev,M. A., Geeves,Michael A., Shnyrov,V. L., Manstein,Dietmar J.
The thermal unfolding of two recombinant fragments of the head of Dictyostelium discoideum myosin II was studied by differential scanning calorimetry. These fragments M754 and M761 correspond to the...
Furch, Marcus, Geeves, Michael A., Manstein, Dietmar J.
The effects of mutations in an actin-binding surface loop of myosin (loop 2) are described. Part of loop 2, the segment between myosin residues 618 and 622, was replaced with sequences enlarged by...
Kinetic Characterization of myosin head fragments with long-lived myosin ATP states (1998)
Friedman, A. L., Geeves, Michael A., Manstein, Dietmar J., Spudich, J. A.
Levitsky, D. I., Ponomarev, M. A., Geeves, Michael A., Shnyrov, V. L., Manstein, Dietmar J.
The thermal unfolding of two recombinant fragments of the head of Dictyostelium discoideum myosin II was studied by differential scanning calorimetry. These fragments M754 and M761 correspond to the...
Myosin motors with artificial lever arms (1996)
Anson,Michael, Geeves,Michael A., Kurzawa,Susanne E., Manstein,Dietmar J.
Myosin motors with artificial lever arms (1996)
Anson, Michael, Geeves, Michael A., Kurzawa, Susanne E., Manstein, Dietmar J.
Kinetic characterization of the catalytic domain of Dictyostelium discoideum myosin (1995)
Woodward,S. K., Geeves,Michael A., Manstein,Dietmar J.
The myosin head consists of a globular motor or catalytic domain that contains both the catalytic and actin binding sites, and a neck region which consists of a 8.5 nm alpha-helix that emerges from...
Cloning vectors for the production of proteins in Dictyostelium discoideum (1995)
Manstein,Dietmar J., Schuster,H. P., Morandini,P., Hunt,D. M.
We constructed and tested a series of cloning vectors designed to facilitate protein production and purification in Dictyostelium discoideum (Dd). These vectors carry the origin of replication of the...
Manstein,Dietmar J., Hunt,D. M.
The eukaryotic organism Dictyostelium discoideum has become one of the organisms of choice for the overexpression of recombinant myosins and myosin fragments. Here, we describe a protocol that...