Didier Fournier

Nanopores: maltoporin channel as a sensor for maltodextrin and lambda-phage. (2006)

Berkane, Emir, Orlik, Frank, Charbit, Alain, Danelon, Christophe, Fournier, Didier, Benz, Roland, ...

BACKGROUND: To harvest nutrition from the outside bacteria e.g. E. coli developed in the outer cell wall a number of sophisticated channels called porins. One of them, maltoporin, is a passive...

Nanopores: maltoporin channel as a sensor for maltodextrin and lambda-phage. (2006)

Berkane, Emir, Orlik, Frank, Charbit, Alain, Danelon, Christophe, Fournier, Didier, Benz, Roland, ...

BACKGROUND: To harvest nutrition from the outside bacteria e.g. E. coli developed in the outer cell wall a number of sophisticated channels called porins. One of them, maltoporin, is a passive...

The effect of engineered disulfide bonds on the stability of Drosophila melanogasteracetylcholinesterase (2006)

Siadat, Omid, Lougarre, Andrée, Lamouroux, Lucille, Ladurantie, Caroline, Fournier, Didier

Background Acetylcholinesterase is irreversibly inhibited by organophosphate and carbamate insecticides allowing its use in biosensors for detection of these insecticides. Drosophila...

Determination of thermodynamic parameters of Xerocomus chrysenteron lectin interactions with N-acetylgalactosamine and Thomsen-Friedenreich antigen by isothermal titration calorimetry (2005)

Damian, Luminita, Fournier, Didier, Winterhalter, Mathias, Paquereau, Laurent

Abstract Background Lectins are carbohydrate-binding proteins which potentially bind to cell surface glycoconjugates. They are found in various organisms including fungi. A lectin from the mushroom...

Liposome retention in size exclusion chromatography (2005)

Ruysschaert, Tristan, Marque, Audrey, Duteyrat, Jean-Luc, Lesieur, Sylviane, Winterhalter, Mathias, Fournier, Didier

Abstract Background Size exclusion chromatography is the method of choice for separating free from liposome-encapsulated molecules. However, if the column is not presaturated with lipids this type of...

Nanopores: maltoporin channel as a sensor for maltodextrin and lambda-phage. (2005)

Berkane, Emir, Orlik, Frank, Charbit, Alain, Danelon, Christophe, Fournier, Didier, Benz, Roland, ...

BACKGROUND: To harvest nutrition from the outside bacteria e.g. E. coli developed in the outer cell wall a number of sophisticated channels called porins. One of them, maltoporin, is a passive...

Mutation of exposed hydrophobic amino acids to arginine to increase protein stability (2004)

Strub, Caroline, Alies, Carole, Lougarre, Andrée, Ladurantie, Caroline, Czaplicki, Jerzy, Fournier, Didier

Abstract Background One strategy to increase the stability of proteins is to reduce the area of water-accessible hydrophobic surface. Results In order to test it, we replaced 14 solvent-exposed...

Acetylcholinesterase alterations reveal the fitness cost of mutations conferring insecticide resistance (2004)

Shi, Ming, Lougarre, Andrée, Alies, Carole, Frémaux, Isabelle, Tang, Zhen, Stojan, Jure, ...

Abstract Background Insecticide resistance is now common in insects due to the frequent use of chemicals to control them, which provides a useful tool to study the adaptation of eukaryotic genome to...

Mutations of acetylcholinesterase which confer insecticide resistance in Drosophila melanogasterpopulations (2004)

Menozzi, Philippe, Shi, Ming, Lougarre, Andrée, Tang, Zhen, Fournier, Didier

Abstract Background Organophosphate and carbamate insecticides irreversibly inhibit acetylcholinesterase causing death of insects. Resistance-modified acetylcholinesterases(AChEs) have been described...

Organophosphorus Insecticides Synergize Pyrethroids in the Resistant Strain of Cotton Bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) from West Africa (2003)

Thibaud Martin, Ochou G. Ochou, Maurice Vaissayre, Didier Fournier

Helicoverpa armigera (Hübner) populations from West Africa recently developed resistance to pyrethroid insecticides through enhanced metabolism by mixed-function oxidases. The combination index...

Improvement of Drosophila acetylcholinesterase stability by elimination of a free cysteine (2002)

Fremaux, Isabelle, Mazères, Serge, Brisson-Lougarre, Andrée, Arnaud, Muriel, Ladurantie, Caroline, Fournier, Didier

Abstract Background Acetylcholinesterase is irreversibly inhibited by organophosphate and carbamate insecticides allowing its use for residue detection with biosensors. Drosophila...

Proteins as Active Compounds Involved in Insecticidal Activity of Mushroom Fruitbodies (2002)

Ming Wang, Véronique Triguéros, Laurent Paquereau, Louis Chavant, Didier Fournier

Many mushrooms are toxic to insects. To identify the chemicals involved in insecticidal activity, the toxicity of 14 species has been studied for water solubility, thermolability, and dialysis. The...

Protein encapsulation in liposomes: efficiency depends on interactions between protein and phospholipid bilayer. (2002)

Colletier, Jacques-Philippe, Chaize, Barnabé, Winterhalter, Mathias, Fournier, Didier

Abstract Background We investigated the encapsulation mechanism of enzymes into liposomes. The existing protocols to achieve high encapsulation efficiencies are basically optimized for chemically...

Detection of Anatoxin-a(s) in Environmental Samples of Cyanobacteria by Using a Biosensor with Engineered Acetylcholinesterases

Devic, Eric, Li, Dunhai, Dauta, Alain, Henriksen, Peter, Codd, Geoffrey A., Marty, Jean-Louis, ...

Bioassays are little used to detect individual toxins in the environment because, compared to analytical methods, these assays are still limited by several problems, such as the sensitivity and...

Protein Splicing of SufB Is Crucial for the Functionality of the Mycobacterium tuberculosis SUF Machinery

Huet, Gaëlle, Castaing, Jean-Philippe, Fournier, Didier, Daffé, Mamadou, Saves, Isabelle

The SufBCD complex is an essential component of the SUF machinery of [Fe-S] cluster biogenesis in many organisms. We show here that in Mycobacterium tuberculosis the formation of this complex is...

Detection of Anatoxin-a(s) in Environmental Samples of Cyanobacteria by Using a Biosensor with Engineered Acetylcholinesterases

Devic, Eric, Li, Dunhai, Dauta, Alain, Henriksen, Peter, Codd, Geoffrey A., Marty, Jean-Louis, ...

Bioassays are little used to detect individual toxins in the environment because, compared to analytical methods, these assays are still limited by several problems, such as the sensitivity and...

Protein Splicing of SufB Is Crucial for the Functionality of the Mycobacterium tuberculosis SUF Machinery

Huet, Gaëlle, Castaing, Jean-Philippe, Fournier, Didier, Daffé, Mamadou, Saves, Isabelle

The SufBCD complex is an essential component of the SUF machinery of [Fe-S] cluster biogenesis in many organisms. We show here that in Mycobacterium tuberculosis the formation of this complex is...

Structural insights into substrate traffic and inhibition in acetylcholinesterase

Colletier, Jacques-Philippe, Fournier, Didier, Greenblatt, Harry M, Stojan, Jure, Sussman, Joel L, Zaccai, Giuseppe, ...

Acetylcholinesterase (AChE) terminates nerve-impulse transmission at cholinergic synapses by rapid hydrolysis of the neurotransmitter, acetylcholine. Substrate traffic in AChE involves at least two...