Scientific Commons: Denis L. J. Lafontaine

Is ribosome synthesis controlled by pol I transcription? (2008)

Chédin, Stéphane, Laferté, Arnaud, Hoang, Tran, Lafontaine, Denis L J, Riva, Michel, Carles, Christophe

Regulation of growth ultimately depends on the control of synthesis of new ribosomes. Ribosome biogenesis is thus a key element of cell biology, which is tightly regulated in response to...

Stable expression in yeast of the mature form of human telomerase RNA depends on its association with the box H/ACA small nucleolar RNP proteins Cbf5p, Nhp2p and Nop10p

Dez, Christophe, Henras, Anthony, Faucon, Bruno, Lafontaine, Denis L. J., Caizergues-Ferrer, Michèle, Henry, Yves

Telomerase is a ribonucleoprotein (RNP) particle required for the replication of telomeres. The RNA component, termed hTR, of human telomerase contains a domain structurally and functionally related...

Pseudouridine Mapping in the Saccharomyces cerevisiae Spliceosomal U Small Nuclear RNAs (snRNAs) Reveals that Pseudouridine Synthase Pus1p Exhibits a Dual Substrate Specificity for U2 snRNA and tRNA

Massenet, Séverine, Motorin, Yuri, Lafontaine, Denis L. J., Hurt, Eduard C., Grosjean, Henri, Branlant, Christiane

Pseudouridine (Ψ) residues were localized in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (UsnRNAs) by using the chemical mapping method. In contrast to vertebrate UsnRNAs, S....

Synthesis and Assembly of the Box C+D Small Nucleolar RNPs

Lafontaine, Denis L. J., Tollervey, David

Two core small nucleolar RNP (snoRNP) proteins, Nop1p (fibrillarin in vertebrates) and Nop58p (also known as Nop5p) have previously been reported to be specifically associated with the box C+D class...

Precursors to the U3 Small Nucleolar RNA Lack Small Nucleolar RNP Proteins but Are Stabilized by La Binding

Kufel, Joanna, Allmang, Christine, Chanfreau, Guillaume, Petfalski, Elisabeth, Lafontaine, Denis L. J., Tollervey, David

Almost all small eukaryotic RNAs are processed from transiently stabilized 3′-extended forms. A key question is how and why such intermediates are stabilized and how they can then be processed to...

Dhr1p, a Putative DEAH-Box RNA Helicase, Is Associated with the Box C+D snoRNP U3

Colley, Alan, Beggs, Jean D., Tollervey, David, Lafontaine, Denis L. J.

Putative RNA helicases are involved in most aspects of gene expression. All previously characterized members of the DEAH-box family of putative RNA helicases are involved in pre-mRNA splicing. Here...

Yeast 18S rRNA Dimethylase Dim1p: a Quality Control Mechanism in Ribosome Synthesis?

Lafontaine, Denis L. J., Preiss, Thomas, Tollervey, David

One of the few rRNA modifications conserved between bacteria and eukaryotes is the base dimethylation present at the 3′ end of the small subunit rRNA. In the yeast Saccharomyces cerevisiae, this...

Box C/D small nucleolar RNA trafficking involves small nucleolar RNP proteins, nucleolar factors and a novel nuclear domain

Verheggen, Céline, Mouaikel, John, Thiry, Marc, Blanchard, Jean-Marie, Tollervey, David, Bordonné, Remi, ...

Nucleolar localization of box C/D small nucleolar (sno) RNAs requires the box C/D motif and, in vertebrates, involves transit through Cajal bodies (CB). We report that in yeast, overexpression of a...

Mammalian and yeast U3 snoRNPs are matured in specific and related nuclear compartments

Verheggen, Céline, Lafontaine, Denis L.J., Samarsky, Dmitry, Mouaikel, John, Blanchard, Jean-Marie, Bordonné, Rémy, ...

Nucleolar localization of vertebrate box C/D snoRNA involves transit through Cajal bodies, but the significance of this event is unkown. To define better the function of this compartment, we analyzed...

The box H+ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase

Lafontaine, Denis L.J., Bousquet-Antonelli, Cécile, Henry, Yves, Caizergues-Ferrer, Michèle, Tollervey, David

Many or all of the sites of pseudouridine (Ψ) formation in eukaryotic rRNA are selected by site-specific base-pairing with members of the box H + ACA class of small nucleolar RNAs (snoRNAs)....

The Small Nucle(ol)ar RNA Cap Trimethyltransferase Is Required for Ribosome Synthesis and Intact Nucleolar Morphology

Colau, Geoffroy, Thiry, Marc, Leduc, Vivian, Bordonné, Rémy, Lafontaine, Denis L. J.

Nucleolar morphogenesis is a poorly defined process. Here we report that the Saccharomyces cerevisiae nucleolar trimethyl guanosine synthase I (Tgs1p), which specifically selects the m7G cap...

Esf2p, a U3-Associated Factor Required for Small-Subunit Processome Assembly and Compaction

Hoang, Tran, Peng, Wen-Tao, Vanrobays, Emmanuel, Krogan, Nevan, Hiley, Shawna, Beyer, Ann L., ...

Esf2p is the Saccharomyces cerevisiae homolog of mouse ABT1, a protein previously identified as a putative partner of the TATA-element binding protein. However, large-scale studies have indicated...

Dim2p, a KH-domain protein required for small ribosomal subunit synthesis

VANROBAYS, EMMANUEL, GÉLUGNE, JEAN-PAUL, CAIZERGUES-FERRER, MICHÈLE, LAFONTAINE, DENIS L.J.

Recent proteomic analyses are revealing the dynamics of preribosome assembly. Following cleavage at processing site A2, which generates the 20S pre-rRNA (the immediate precursor to the 18S rRNA),...