Eukaryotic ribonuclease P: Increased complexity to cope with the nuclear pre-tRNA pathway (2001)
Xiao, Shaohua, Houser-Scott, Felicia, Engelke, David R.
Ribonuclease P is an ancient enzyme that cleaves pre-tRNAs to generate mature 5' ends. It contains an essential RNA subunit in Bacteria, Archaea, and Eukarya, but the degree to which the RNA subunit...
Structure-function analysis in nuclear RNase P RNA (1995)
Tranguch, Anthony J., Engelke, David R., Pagán-Ramos, Eileen
Eukaryotic ribonuclease P (RNase P) enzymes require both RNA and protein subunits for activity in vivo and in vitro . We have undertaken an analysis of the complex RNA subunit of the nuclear...
Yeast expression vectors using RNA polymerase III promoters (1994)
Good, Paul D., Engelke, David R.
A series of Saccharomyces cerevisiae -- Escherichia coli shuttle vectors is described in which small RNAs can be stably expressed in yeast from two different promoters for RNA polymerase III...
Evans, Claire F., Engelke, David R.
Complexes between transcription factor TFIIIC and eukaryotic tRNA gene internal promoter A and B boxes are unusual in that the binding to the two distinct sites tolerates considerable variation in...
Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli (1990)
Engelke, David R., Krikos, Alexandra, Bruck, Mary E., Ginsburg, David
DNA polymerase from Thermus aquaticus has become a common reagent in molecular biology because of its utility in DNA amplification and DNA sequencing protocols. A simplified method is described here...
Initiation of rrn transcription in chloroplasts of Euglena gracilis bacillaris (1988)
McGarvey, Peter, Lee, Jae-Yong, El-Gewely, M. Raafat, Helling, Robert B., Engelke, David R.
The site of initiation of chloroplast rRNA synthesis was determined by Sl-mapping and by sequencing primary rRNA transcripts specifically labeled at their 5′-end. Transcription initiates at a...
Interaction of tRNA transcription factors with satellite I DNA from Xenopus laevis (1988)
A cloned repeat of Xenopus laevis satellite I DNA was tested for the ability to form stable complexes with tRNA transcription factors in vitro. In template exclusion studies, the satellite I DNA...
Direct identification of small sequence changes in chromosomal DNA (1986)
Huibregtse, Jon M., Engelke, David R.
Dideoxynucleotide chain termination sequencing has been applied directly to genomic DNA templates by annealing radiolabeled oligodeoxynucleotide primers to unique sites in total yeast DNA and...
DNA ligands that bind tightly and selectively to cellobiose
Yang, Qing, Goldstein, Irwin J., Mei, Houng-Yau, Engelke, David R.
Cell surface oligosaccharides have been shown to play essential biological roles in such diverse biological phenomena as cellular adhesion, molecular recognition, and inflammatory response. The...
Kendall, Ann, Hull, Melissa W., Bertrand, Edouard, Good, Paul D., Singer, Robert H., Engelke, David R.
In the budding yeast, Saccharomyces cerevisiae, actively transcribed tRNA genes can negatively regulate adjacent RNA polymerase II (pol II)-transcribed promoters. This tRNA gene-mediated silencing is...
Sephadex-binding RNA ligands: rapid affinity purification of RNA from complex RNA mixtures
Srisawat, Chatchawan, Goldstein, Irwin J., Engelke, David R.
Sephadex-binding RNA ligands (aptamers) were obtained through in vitro selection. They could be classified into two groups based on their consensus sequences and the aptamers from both groups showed...
5′ Processing of tRNA Precursors Can Be Modulated by the Human La Antigen Phosphoprotein†
Fan, Hao, Goodier, John L., Chamberlain, Joel R., Engelke, David R., Maraia, Richard J.
Eukaryotic precursor (pre)-tRNAs are processed at both ends prior to maturation. Pre-tRNAs and other nascent transcripts synthesized by RNA polymerase III are bound at their 3′ ends at the sequence...
Browning, Catherine M., Cagnon, Laurence, Good, Paul D., Rossi, John, Engelke, David R., Markovitz, David M.
Tat activation-response region (TAR) decoys have been developed for use in gene therapy for people infected with human immunodeficiency virus type 1 (HIV-1). When a TAR RNA decoy is overexpressed, it...
Interactions among the protein and RNA subunits of Saccharomyces cerevisiae nuclear RNase P
Houser-Scott, Felicia, Xiao, Shaohua, Millikin, Christopher E., Zengel, Janice M., Lindahl, Lasse, Engelke, David R.
Ribonuclease P (RNase P) is a ubiquitous endoribonuclease that cleaves precursor tRNAs to generate mature 5′ termini. Although RNase P from all kingdoms of life have been found to have essential...
Chamberlain, Joel R., Lee, Yoon, Lane, William S., Engelke, David R.
Ribonuclease P (RNase P) is a ribonucleoprotein enzyme that cleaves precursor tRNA transcripts to give mature 5′ ends. RNase P in eubacteria has a large, catalytic RNA subunit and a small protein...
Nucleolar localization of early tRNA processing
Bertrand, Edouard, Houser-Scott, Felicia, Kendall, Ann, Singer, Robert H., Engelke, David R.
There is little information as to the location of early tRNA biosynthesis. Using fluorescent in situ hybridization in the budding yeast, Saccharomyces cerevisiae, examples of nuclear pre-tRNAs are...
An active precursor in assembly of yeast nuclear ribonuclease P.
Srisawat, Chatchawan, Houser-Scott, Felicia, Bertrand, Edouard, Xiao, Shaohua, Singer, Robert H, Engelke, David R
The RNA-protein subunit assembly of nuclear RNase P was investigated by specific isolation and characterization of the precursor and mature forms of RNase P using an RNA affinity ligand. Pre-RNase P...
XIAO, SHAOHUA, DAY-STORMS, JEREMY J., SRISAWAT, CHATCHAWAN, FIERKE, CAROL A., ENGELKE, DAVID R.
RNase P is a ubiquitous endoribonuclease responsible for cleavage of the 5′ leader of precursor tRNAs (pre-tRNAs). Although the protein composition of RNase P holoenzymes varies significantly among...
The RNA Ontology Consortium: An open invitation to the RNA community
LEONTIS, NEOCLES B., ALTMAN, RUSS B., BERMAN, HELEN M., BRENNER, STEVEN E., BROWN, JAMES W., ENGELKE, DAVID R., ...
The aim of the RNA Ontology Consortium (ROC) is to create an integrated conceptual framework—an RNA Ontology (RO)—with a common, dynamic, controlled, and structured vocabulary to describe and...
Xiao, Shaohua, Hsieh, John, Nugent, Rebecca L., Coughlin, Daniel J., Fierke, Carol A., Engelke, David R.
RNase P and RNase MRP are ribonucleoprotein enzymes required for 5′-end maturation of precursor tRNAs (pre-tRNAs) and processing of precursor ribosomal RNAs, respectively. In yeast, RNase P and MRP...
DNA ligands that bind tightly and selectively to cellobiose
Yang, Qing, Goldstein, Irwin J., Mei, Houng-Yau, Engelke, David R.
Cell surface oligosaccharides have been shown to play essential biological roles in such diverse biological phenomena as cellular adhesion, molecular recognition, and inflammatory response. The...
Kendall, Ann, Hull, Melissa W., Bertrand, Edouard, Good, Paul D., Singer, Robert H., Engelke, David R.
In the budding yeast, Saccharomyces cerevisiae, actively transcribed tRNA genes can negatively regulate adjacent RNA polymerase II (pol II)-transcribed promoters. This tRNA gene-mediated silencing is...
Sephadex-binding RNA ligands: rapid affinity purification of RNA from complex RNA mixtures
Srisawat, Chatchawan, Goldstein, Irwin J., Engelke, David R.
Sephadex-binding RNA ligands (aptamers) were obtained through in vitro selection. They could be classified into two groups based on their consensus sequences and the aptamers from both groups showed...
5′ Processing of tRNA Precursors Can Be Modulated by the Human La Antigen Phosphoprotein†
Fan, Hao, Goodier, John L., Chamberlain, Joel R., Engelke, David R., Maraia, Richard J.
Eukaryotic precursor (pre)-tRNAs are processed at both ends prior to maturation. Pre-tRNAs and other nascent transcripts synthesized by RNA polymerase III are bound at their 3′ ends at the sequence...
Browning, Catherine M., Cagnon, Laurence, Good, Paul D., Rossi, John, Engelke, David R., Markovitz, David M.
Tat activation-response region (TAR) decoys have been developed for use in gene therapy for people infected with human immunodeficiency virus type 1 (HIV-1). When a TAR RNA decoy is overexpressed, it...
Interactions among the protein and RNA subunits of Saccharomyces cerevisiae nuclear RNase P
Houser-Scott, Felicia, Xiao, Shaohua, Millikin, Christopher E., Zengel, Janice M., Lindahl, Lasse, Engelke, David R.
Ribonuclease P (RNase P) is a ubiquitous endoribonuclease that cleaves precursor tRNAs to generate mature 5′ termini. Although RNase P from all kingdoms of life have been found to have essential...
Chamberlain, Joel R., Lee, Yoon, Lane, William S., Engelke, David R.
Ribonuclease P (RNase P) is a ribonucleoprotein enzyme that cleaves precursor tRNA transcripts to give mature 5′ ends. RNase P in eubacteria has a large, catalytic RNA subunit and a small protein...
Nucleolar localization of early tRNA processing
Bertrand, Edouard, Houser-Scott, Felicia, Kendall, Ann, Singer, Robert H., Engelke, David R.
There is little information as to the location of early tRNA biosynthesis. Using fluorescent in situ hybridization in the budding yeast, Saccharomyces cerevisiae, examples of nuclear pre-tRNAs are...
An active precursor in assembly of yeast nuclear ribonuclease P.
Srisawat, Chatchawan, Houser-Scott, Felicia, Bertrand, Edouard, Xiao, Shaohua, Singer, Robert H, Engelke, David R
The RNA-protein subunit assembly of nuclear RNase P was investigated by specific isolation and characterization of the precursor and mature forms of RNase P using an RNA affinity ligand. Pre-RNase P...
XIAO, SHAOHUA, DAY-STORMS, JEREMY J., SRISAWAT, CHATCHAWAN, FIERKE, CAROL A., ENGELKE, DAVID R.
RNase P is a ubiquitous endoribonuclease responsible for cleavage of the 5′ leader of precursor tRNAs (pre-tRNAs). Although the protein composition of RNase P holoenzymes varies significantly among...
The RNA Ontology Consortium: An open invitation to the RNA community
LEONTIS, NEOCLES B., ALTMAN, RUSS B., BERMAN, HELEN M., BRENNER, STEVEN E., BROWN, JAMES W., ENGELKE, DAVID R., ...
The aim of the RNA Ontology Consortium (ROC) is to create an integrated conceptual framework—an RNA Ontology (RO)—with a common, dynamic, controlled, and structured vocabulary to describe and...
Xiao, Shaohua, Hsieh, John, Nugent, Rebecca L., Coughlin, Daniel J., Fierke, Carol A., Engelke, David R.
RNase P and RNase MRP are ribonucleoprotein enzymes required for 5′-end maturation of precursor tRNAs (pre-tRNAs) and processing of precursor ribosomal RNAs, respectively. In yeast, RNase P and MRP...
Protein kinase A regulates RNA polymerase III transcription through the nuclear localization of Maf1
Moir, Robyn D., Lee, JaeHoon, Haeusler, Rebecca A., Desai, Neelam, Engelke, David R., Willis, Ian M.
Maf1 is an essential and specific mediator of transcriptional repression in the RNA polymerase (pol) III system. Maf1-dependent repression occurs in response to a wide range of conditions, suggesting...
Spatial organization of transcription by RNA polymerase III
Haeusler, Rebecca A., Engelke, David R.
RNA polymerase III (pol III) transcribes many essential, small, noncoding RNAs, including the 5S rRNAs and tRNAs. While most pol III-transcribed genes are found scattered throughout the linear...
Aspinall, Tanya V., Gordon, James M.B., Bennett, Hayley J., Karahalios, Panagiotis, Bukowski, John-Paul, Walker, Scott C., ...
Ribonuclease MRP is an endonuclease, related to RNase P, which functions in eukaryotic pre-rRNA processing. In Saccharomyces cerevisiae, RNase MRP comprises an RNA subunit and ten proteins. To...