Transcriptional Silencing of Multiple Genes in Trophozoites of Entamoeba histolytica (2006)
Rivka Bracha, Yael Nuchamowitz, Michael Anbar, David Mirelman
In a previous work we described the transcriptional silencing of the amoebapore A (AP-A) gene (Ehap-a) of Entamoeba histolytica strain HM-1:IMSS. The silencing occurred following transfection with a...
LOUIS S. DIAMOND, DAVID MIRELMAN
Entamoeba histolytica Schaudinn, 1903 and Entamoeba dispar Brumpt, 1925 are two of eight species of Entamoeba that sometimes inhabit the human colon. The former is an invasive organism capable of...
Ankri, Serge, Stolarsky, Tamara, Bracha, Rivka, Padilla-Vaca, Felipe, Mirelman, David
Trophozoites of virulent Entamoeba histolytica transfected with the antisense gene encoding cysteine proteinase 5 (CP5) have only 10% of the CP activity but retain their cytopathic activity on...
Padilla-Vaca, Felipe, Ankri, Serge, Bracha, Rivka, Koole, Lucy Anna, Mirelman, David
Entamoeba histolytica virulence is related to a number of amebic components (lectins, cysteine proteinases, and amebapore) and host factors, such as intestinal bacterial flora. Trophozoites are...
Katz, Uriel, Ankri, Serge, Stolarsky, Tamara, Nuchamowitz, Yael, Mirelman, David
The 260-kDa heterodimeric Gal/GalNAc-specific Lectin (Gal-lectin) of Entamoeba histolytica dissociates under reducing conditions into a heavy (hgl, 170 kDa) and a light subunit (lgl, 35 kDa). We have...
Bracha, Rivka, Nuchamowitz, Yael, Mirelman, David
Transcriptional silencing of the gene coding for amoebapore A (AP-A) was observed when trophozoites of Entamoeba histolytica were transfected with a hybrid plasmid construct containing the ap-a gene...
Nature and Origins of Phosphorus Compounds in Isolated Cell Walls of Staphylococcus aureus
Mirelman, David, Shaw, D. R. D., Park, James T.
Preparations of purified cell walls from Staphylococcus aureus were shown to contain small amounts of phospholipid and glycerol teichoic acid. Since these are components of the cell membrane, it is...
Properties of a Novel Pleiotropic Bacteriophage-Resistant Mutant of Staphylococcus aureus H1
Chatterjee, Anadi N., Mirelman, David, Singer, Howard J., Park, James T.
A phage-resistant mutant of Staphylococcus aureus H (SmR), S. aureus 52A5, was previously shown to lack polymeric teichoic acid. This paper characterizes other phenotypic differences between the...
Improved Filtration Technique for Concentrating and Harvesting Bacteria
Tanny, Gerald B., Mirelman, David, Pistole, Thomas
An improved technique is described for the filtrative concentration and harvesting of bacterial cultures. A pleated tangential flow filtration unit containing 1,000 cm2 of 0.2-μm-pore-size...
Zhang, Xiaochun, Zhang, Zhi, Alexander, Diane, Bracha, Rivka, Mirelman, David, Stanley, Samuel L.
Entamoeba histolytica trophozoites produce amoebapores, a family of small amphipathic peptides capable of insertion into bacterial or eukaryotic membranes and causing cellular lysis. Recently, E....
Mirelman, David, Bracha, Rivka, Sharon, Nathan
Cell-wall preparations of Micrococcus luteus (lysodeikticus) catalyze in vitro peptidoglycan synthesis from UDP N-acetyl-D-glucosamine, UDP N-acetylmuramic acid-pentapeptide, and glycine. Newly...
Mirelman, David, Bracha, Rivka
Penicillin G was found to inhibit the formation of the d-alanyl-l-alanine cross-linkage in intact cells of Micrococcus luteus. This reaction was approximately 50-fold less susceptible to penicillin...
Lectin Activity in Entamoeba histolytica trophozoites
Kobiler, David, Mirelman, David
A lectin (carbohydrate-binding protein) has been found in extracts of a number of axenically grown trophozoites of Entamoeba histolytica strains. The strains grown in TYI-S-33 medium (Diamond et al.,...
Bar-Shavit, Zvi, Goldman, Rachel, Ofek, Itzhak, Sharon, Nathan, Mirelman, David
Recently, it was suggested that a mannose-specific lectin on the bacterial cell surface is responsible for the recognition by phagocytic cells of certain nonopsonized Escherichia coli strains. In...
Anbar, Michael, Bracha, Rivka, Nuchamowitz, Yael, Li, Yan, Florentin, Anat, Mirelman, David
Transcriptional silencing of an amoebapore (ap-a) gene occurred in Entamoeba histolytica following the transfection of plasmids containing a DNA segment (473 bp) homologous to the 5′ upstream...
Transcriptional Silencing of Multiple Genes in Trophozoites of Entamoeba histolytica
Bracha, Rivka, Nuchamowitz, Yael, Anbar, Michael, Mirelman, David
In a previous work we described the transcriptional silencing of the amoebapore A (AP-A) gene (Ehap-a) of Entamoeba histolytica strain HM-1:IMSS. The silencing occurred following transfection with a...
Weber, Christian, Guigon, Ghislaine, Bouchier, Christiane, Frangeul, Lionel, Moreira, Sandrine, Sismeiro, Odile, ...
Gene expression analysis by microarray assay revealed that when exposed to stress, Entamoeba histolytica exhibits a specific heat shock response, together with a dramatic overall reduction in gene...
Antimalarial Activity of Allicin, a Biologically Active Compound from Garlic Cloves
Coppi, Alida, Cabinian, Melissa, Mirelman, David, Sinnis, Photini
The incidence of malaria is increasing, and there is an urgent need to identify new drug targets for both prophylaxis and chemotherapy. Potential new drug targets include Plasmodium proteases that...
Ankri, Serge, Stolarsky, Tamara, Bracha, Rivka, Padilla-Vaca, Felipe, Mirelman, David
Trophozoites of virulent Entamoeba histolytica transfected with the antisense gene encoding cysteine proteinase 5 (CP5) have only 10% of the CP activity but retain their cytopathic activity on...
Padilla-Vaca, Felipe, Ankri, Serge, Bracha, Rivka, Koole, Lucy Anna, Mirelman, David
Entamoeba histolytica virulence is related to a number of amebic components (lectins, cysteine proteinases, and amebapore) and host factors, such as intestinal bacterial flora. Trophozoites are...
Katz, Uriel, Ankri, Serge, Stolarsky, Tamara, Nuchamowitz, Yael, Mirelman, David
The 260-kDa heterodimeric Gal/GalNAc-specific Lectin (Gal-lectin) of Entamoeba histolytica dissociates under reducing conditions into a heavy (hgl, 170 kDa) and a light subunit (lgl, 35 kDa). We have...
Bracha, Rivka, Nuchamowitz, Yael, Mirelman, David
Transcriptional silencing of the gene coding for amoebapore A (AP-A) was observed when trophozoites of Entamoeba histolytica were transfected with a hybrid plasmid construct containing the ap-a gene...
Nature and Origins of Phosphorus Compounds in Isolated Cell Walls of Staphylococcus aureus
Mirelman, David, Shaw, D. R. D., Park, James T.
Preparations of purified cell walls from Staphylococcus aureus were shown to contain small amounts of phospholipid and glycerol teichoic acid. Since these are components of the cell membrane, it is...
Properties of a Novel Pleiotropic Bacteriophage-Resistant Mutant of Staphylococcus aureus H1
Chatterjee, Anadi N., Mirelman, David, Singer, Howard J., Park, James T.
A phage-resistant mutant of Staphylococcus aureus H (SmR), S. aureus 52A5, was previously shown to lack polymeric teichoic acid. This paper characterizes other phenotypic differences between the...
Improved Filtration Technique for Concentrating and Harvesting Bacteria
Tanny, Gerald B., Mirelman, David, Pistole, Thomas
An improved technique is described for the filtrative concentration and harvesting of bacterial cultures. A pleated tangential flow filtration unit containing 1,000 cm2 of 0.2-μm-pore-size...
Zhang, Xiaochun, Zhang, Zhi, Alexander, Diane, Bracha, Rivka, Mirelman, David, Stanley, Samuel L.
Entamoeba histolytica trophozoites produce amoebapores, a family of small amphipathic peptides capable of insertion into bacterial or eukaryotic membranes and causing cellular lysis. Recently, E....
Mirelman, David, Bracha, Rivka, Sharon, Nathan
Cell-wall preparations of Micrococcus luteus (lysodeikticus) catalyze in vitro peptidoglycan synthesis from UDP N-acetyl-D-glucosamine, UDP N-acetylmuramic acid-pentapeptide, and glycine. Newly...
Mirelman, David, Bracha, Rivka
Penicillin G was found to inhibit the formation of the d-alanyl-l-alanine cross-linkage in intact cells of Micrococcus luteus. This reaction was approximately 50-fold less susceptible to penicillin...
Lectin Activity in Entamoeba histolytica trophozoites
Kobiler, David, Mirelman, David
A lectin (carbohydrate-binding protein) has been found in extracts of a number of axenically grown trophozoites of Entamoeba histolytica strains. The strains grown in TYI-S-33 medium (Diamond et al.,...
Bar-Shavit, Zvi, Goldman, Rachel, Ofek, Itzhak, Sharon, Nathan, Mirelman, David
Recently, it was suggested that a mannose-specific lectin on the bacterial cell surface is responsible for the recognition by phagocytic cells of certain nonopsonized Escherichia coli strains. In...
Anbar, Michael, Bracha, Rivka, Nuchamowitz, Yael, Li, Yan, Florentin, Anat, Mirelman, David
Transcriptional silencing of an amoebapore (ap-a) gene occurred in Entamoeba histolytica following the transfection of plasmids containing a DNA segment (473 bp) homologous to the 5′ upstream...
Weber, Christian, Guigon, Ghislaine, Bouchier, Christiane, Frangeul, Lionel, Moreira, Sandrine, Sismeiro, Odile, ...
Gene expression analysis by microarray assay revealed that when exposed to stress, Entamoeba histolytica exhibits a specific heat shock response, together with a dramatic overall reduction in gene...
Transcriptional Silencing of Multiple Genes in Trophozoites of Entamoeba histolytica
Bracha, Rivka, Nuchamowitz, Yael, Anbar, Michael, Mirelman, David
In a previous work we described the transcriptional silencing of the amoebapore A (AP-A) gene (Ehap-a) of Entamoeba histolytica strain HM-1:IMSS. The silencing occurred following transfection with a...
Antimalarial Activity of Allicin, a Biologically Active Compound from Garlic Cloves
Coppi, Alida, Cabinian, Melissa, Mirelman, David, Sinnis, Photini
The incidence of malaria is increasing, and there is an urgent need to identify new drug targets for both prophylaxis and chemotherapy. Potential new drug targets include Plasmodium proteases that...
Sela, Uri, Ganor, Sharon, Hecht, Iris, Brill, Alexander, Miron, Talia, Rabinkov, Aharon, ...
Allicin, a major ingredient of fresh garlic extract that is produced during the crushing of garlic cloves, exerts various beneficial biological effects, including a broad spectrum of antimicrobial...
EhLimA, a Novel LIM Protein, Localizes to the Plasma Membrane in Entamoeba histolytica▿
Wender, Nomy, Villalobo, Eduardo, Mirelman, David
The parasitic protozoan Entamoeba histolytica relies on a very dynamic cytoskeleton in order to invade and survive in host tissues. Identification of cytoskeletal elements is key to understanding...
Bracha, Rivka, Nuchamowitz, Yael, Wender, Nomy, Mirelman, David
The Entamoeba histolytica cell surface Gal/GalNAc-inhibitable lectin is a heterodimer between a heavy (170 kDa) subunit linked via a disulfide bond to a light (31 to 35 kDa) subunit. Five light...