David M. Segal

Secreted MD-2 is a large polymeric protein that efficiently confers lipopolysaccharide sensitivity to Toll-like receptor 4

Visintin, Alberto, Mazzoni, Alessandra, Spitzer, Jessica A., Segal, David M.

Toll-like receptor 4 (TLR4), the principal signaling receptor for lipopolysaccharide (LPS) in mammals, requires the binding of MD-2 to its extracellular domain for maximal responsiveness. MD-2...

Enhancement of Adenovirus Vector Entry into CD70-Positive B-Cell Lines by Using a Bispecific CD70-Adenovirus Fiber Antibody

Israel, Bruce F., Pickles, Ray J., Segal, David M., Gerard, Robert D., Kenney, Shannon C.

Although many recombinant adenovirus vectors (rAd) have been developed, especially by using group C adenoviruses, to transfer and express genes, such rAd do not readily infect B-cell lines due to the...

The role of disulfide bonds in the assembly and function of MD-2

Mullen, Gregory E. D., Kennedy, Margaret N., Visintin, Alberto, Mazzoni, Alessandra, Leifer, Cynthia A., Davies, David R., ...

MD-2 is a secreted glycoprotein that binds to the extracellular domain of Toll-like receptor 4 (TLR4) and is required for the activation of TLR4 by lipopolysaccharide (LPS). The protein contains...

Histamine regulates cytokine production in maturing dendritic cells, resulting in altered T cell polarization

Mazzoni, Alessandra, Young, Howard A., Spitzer, Jessica H., Visintin, Alberto, Segal, David M.

Atopic diseases such as allergy and asthma are characterized by increases in Th2 cells and serum IgE antibodies. The binding of allergens to IgE on mast cells triggers the release of several...

Crystals of Phosphorylcholine-Binding Fab-Fragments from Mouse Myeloma Proteins: Preparation and X-Ray Analysis

Rudikoff, Stuart, Potter, Michael, Segal, David M., Padlan, Eduardo A., Davies, David R.

Fab-fragments of several phosphorylcholine-binding mouse-myeloma proteins have been prepared by pepsin digestion; two of these, MOPC 167 and McPC 603, gave large crystals from ammonium sulfate...

The Three-Dimensional Structure of a Phosphorylcholine-Binding Mouse Immunoglobulin Fab and the Nature of the Antigen Binding Site

Segal, David M., Padlan, Eduardo A., Cohen, Gerson H., Rudikoff, Stuart, Potter, Michael, Davies, David R.

The structure of the Fab of McPC 603, a mouse myeloma protein with phosphorylcholine binding activity, has been determined to 3.1-Å resoltuion. The four domains are found to be structurally similar...

The molecular structure of the Toll-like receptor 3 ligand-binding domain

Bell, Jessica K., Botos, Istvan, Hall, Pamela R., Askins, Janine, Shiloach, Joseph, Segal, David M., ...

Innate immunity is the first line of defense against invading pathogens. Toll-like receptors (TLRs) act as sentinels of the innate immune system, sensing a variety of ligands from lipopolysaccharide...

The dsRNA binding site of human Toll-like receptor 3

Bell, Jessica K., Askins, Janine, Hall, Pamela R., Davies, David R., Segal, David M.

Pathogen recognition by Toll-like receptors (TLRs) initiates innate immune responses that are essential for inhibiting pathogen dissemination and for the development of acquired immunity. The TLRs...

Secreted MD-2 is a large polymeric protein that efficiently confers lipopolysaccharide sensitivity to Toll-like receptor 4

Visintin, Alberto, Mazzoni, Alessandra, Spitzer, Jessica A., Segal, David M.

Toll-like receptor 4 (TLR4), the principal signaling receptor for lipopolysaccharide (LPS) in mammals, requires the binding of MD-2 to its extracellular domain for maximal responsiveness. MD-2...

Enhancement of Adenovirus Vector Entry into CD70-Positive B-Cell Lines by Using a Bispecific CD70-Adenovirus Fiber Antibody

Israel, Bruce F., Pickles, Ray J., Segal, David M., Gerard, Robert D., Kenney, Shannon C.

Although many recombinant adenovirus vectors (rAd) have been developed, especially by using group C adenoviruses, to transfer and express genes, such rAd do not readily infect B-cell lines due to the...

The role of disulfide bonds in the assembly and function of MD-2

Mullen, Gregory E. D., Kennedy, Margaret N., Visintin, Alberto, Mazzoni, Alessandra, Leifer, Cynthia A., Davies, David R., ...

MD-2 is a secreted glycoprotein that binds to the extracellular domain of Toll-like receptor 4 (TLR4) and is required for the activation of TLR4 by lipopolysaccharide (LPS). The protein contains...

Histamine regulates cytokine production in maturing dendritic cells, resulting in altered T cell polarization

Mazzoni, Alessandra, Young, Howard A., Spitzer, Jessica H., Visintin, Alberto, Segal, David M.

Atopic diseases such as allergy and asthma are characterized by increases in Th2 cells and serum IgE antibodies. The binding of allergens to IgE on mast cells triggers the release of several...

Crystals of Phosphorylcholine-Binding Fab-Fragments from Mouse Myeloma Proteins: Preparation and X-Ray Analysis

Rudikoff, Stuart, Potter, Michael, Segal, David M., Padlan, Eduardo A., Davies, David R.

Fab-fragments of several phosphorylcholine-binding mouse-myeloma proteins have been prepared by pepsin digestion; two of these, MOPC 167 and McPC 603, gave large crystals from ammonium sulfate...

The Three-Dimensional Structure of a Phosphorylcholine-Binding Mouse Immunoglobulin Fab and the Nature of the Antigen Binding Site

Segal, David M., Padlan, Eduardo A., Cohen, Gerson H., Rudikoff, Stuart, Potter, Michael, Davies, David R.

The structure of the Fab of McPC 603, a mouse myeloma protein with phosphorylcholine binding activity, has been determined to 3.1-Å resoltuion. The four domains are found to be structurally similar...

The molecular structure of the Toll-like receptor 3 ligand-binding domain

Bell, Jessica K., Botos, Istvan, Hall, Pamela R., Askins, Janine, Shiloach, Joseph, Segal, David M., ...

Innate immunity is the first line of defense against invading pathogens. Toll-like receptors (TLRs) act as sentinels of the innate immune system, sensing a variety of ligands from lipopolysaccharide...

The dsRNA binding site of human Toll-like receptor 3

Bell, Jessica K., Askins, Janine, Hall, Pamela R., Davies, David R., Segal, David M.

Pathogen recognition by Toll-like receptors (TLRs) initiates innate immune responses that are essential for inhibiting pathogen dissemination and for the development of acquired immunity. The TLRs...

The TLR3 signaling complex forms by cooperative receptor dimerization

Leonard, Joshua N., Ghirlando, Rodolfo, Askins, Janine, Bell, Jessica K., Margulies, David H., Davies, David R., ...

Toll-like receptors (TLRs) initiate immune responses by recognizing pathogen-associated molecules, but the molecular basis for recognition is poorly understood. In particular, it is unclear how...