CellProfiler: image analysis software for identifying and quantifying cell phenotypes (2006)
Carpenter, Anne E, Jones, Thouis R, Lamprecht, Michael R, Clarke, Colin, Kang, In, Friman, Ola, ...
Abstract Biologists can now prepare and image thousands of samples per day using automation, enabling chemical screens and functional genomics (for example, using RNA interference). Here we describe...
Minimizing the risk of reporting false positives in large-scale RNAi screens (2006)
Echeverri, Christophe J, Beachy, Philip A, Baum, Buzz, Boutros, Michael, Buchholz, Frank, Chanda, Sumit K, ...
Large-scale RNA interference (RNAi)-based analyses, very much as other 'omic' approaches, have inherent rates of false positives and negatives. The variability in the standards of care applied to...
RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1
Burnett, Patrick E., Barrow, Roxanne K., Cohen, Noam A., Snyder, Solomon H., Sabatini, David M.
The complex of rapamycin with its intracellular receptor, FKBP12, interacts with RAFT1/FRAP/mTOR, the in vivo rapamycin-sensitive target and a member of the ataxia telangiectasia mutated...
Neurabin is a synaptic protein linking p70 S6 kinase and the neuronal cytoskeleton
Burnett, Patrick E., Blackshaw, Seth, Lai, Michael M., Qureshi, Irfan A., Burnett, Aaron F., Sabatini, David M., ...
p70 S6 kinase (p70S6k) is a mitogen-activated protein kinase that plays a central role in the control of mRNA translation. It physiologically phosphorylates the S6 protein of the 40s ribosomal...
Enumeration of the Simian Virus 40 Early Region Elements Necessary for Human Cell Transformation
Hahn, William C., Dessain, Scott K., Brooks, Mary W., King, Jessie E., Elenbaas, Brian, Sabatini, David M., ...
While it is clear that cancer arises from the accumulation of genetic mutations that endow the malignant cell with the properties of uncontrolled growth and proliferation, the precise combinations of...
Enumeration of the Simian Virus 40 Early Region Elements Necessary for Human Cell Transformation
Hahn, William C., Dessain, Scott K., Brooks, Mary W., King, Jessie E., Elenbaas, Brian, Sabatini, David M., ...
Peng, Tao, Golub, Todd R., Sabatini, David M.
RAFT1/FRAP/mTOR is a key regulator of cell growth and division and the mammalian target of rapamycin, an immunosuppressive and anticancer drug. Rapamycin deprivation and nutrient deprivation have...
Bailey, Steve N., Sabatini, David M., Stockwell, Brent R.
We developed a microarray-based system for screening small molecules in mammalian cells. This system is compatible with image-based screens and requires fewer than 100 cells per compound. Each...
Lentivirus-delivered stable gene silencing by RNAi in primary cells
STEWART, SHEILA A., DYKXHOORN, DEREK M., PALLISER, DEBORAH, MIZUNO, HANA, YU, EVAN Y., AN, DONG SUNG, ...
Genome-wide genetic approaches have proven useful for examining pathways of biological significance in model organisms such as Saccharomyces cerevisiae, Drosophila melanogastor, and Caenorhabditis...
RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1
Burnett, Patrick E., Barrow, Roxanne K., Cohen, Noam A., Snyder, Solomon H., Sabatini, David M.
The complex of rapamycin with its intracellular receptor, FKBP12, interacts with RAFT1/FRAP/mTOR, the in vivo rapamycin-sensitive target and a member of the ataxia telangiectasia mutated...
Neurabin is a synaptic protein linking p70 S6 kinase and the neuronal cytoskeleton
Burnett, Patrick E., Blackshaw, Seth, Lai, Michael M., Qureshi, Irfan A., Burnett, Aaron F., Sabatini, David M., ...
p70 S6 kinase (p70S6k) is a mitogen-activated protein kinase that plays a central role in the control of mRNA translation. It physiologically phosphorylates the S6 protein of the 40s ribosomal...
Enumeration of the Simian Virus 40 Early Region Elements Necessary for Human Cell Transformation
Hahn, William C., Dessain, Scott K., Brooks, Mary W., King, Jessie E., Elenbaas, Brian, Sabatini, David M., ...
While it is clear that cancer arises from the accumulation of genetic mutations that endow the malignant cell with the properties of uncontrolled growth and proliferation, the precise combinations of...
Enumeration of the Simian Virus 40 Early Region Elements Necessary for Human Cell Transformation
Hahn, William C., Dessain, Scott K., Brooks, Mary W., King, Jessie E., Elenbaas, Brian, Sabatini, David M., ...
Peng, Tao, Golub, Todd R., Sabatini, David M.
RAFT1/FRAP/mTOR is a key regulator of cell growth and division and the mammalian target of rapamycin, an immunosuppressive and anticancer drug. Rapamycin deprivation and nutrient deprivation have...
Bailey, Steve N., Sabatini, David M., Stockwell, Brent R.
We developed a microarray-based system for screening small molecules in mammalian cells. This system is compatible with image-based screens and requires fewer than 100 cells per compound. Each...
Lentivirus-delivered stable gene silencing by RNAi in primary cells
STEWART, SHEILA A., DYKXHOORN, DEREK M., PALLISER, DEBORAH, MIZUNO, HANA, YU, EVAN Y., AN, DONG SUNG, ...
Genome-wide genetic approaches have proven useful for examining pathways of biological significance in model organisms such as Saccharomyces cerevisiae, Drosophila melanogastor, and Caenorhabditis...
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Carpenter, Anne E, Jones, Thouis R, Lamprecht, Michael R, Clarke, Colin, Kang, In Han, Friman, Ola, ...
CellProfiler, the first free, open-source system for flexible and high-throughput cell image analysis is described.
Rapamycin derivatives reduce mTORC2 signaling and inhibit AKT activation in AML
Zeng, Zhihong, Sarbassov, Dos D., Samudio, Ismael J., Yee, Karen W. L., Munsell, Mark F., Ellen Jackson, C., ...
The mTOR complex 2 (mTORC2) containing mTOR and rictor is thought to be rapamycin insensitive and was recently shown to regulate the prosurvival kinase AKT by phosphorylation on Ser473. We...