David Donze

The Boundaries of the Silenced (2001)

David Donze, Christopher R. Adams, Rohinton T. Kamakaka

omatin, which compacts the length of the chromosomal DNA molecule into a substantially more condensed form. This compaction involves a hierarchical level of organization in which the first and second...

Sir2p exists in two nucleosome-binding complexes with distinct deacetylase activities

Ghidelli, Sonja, Donze, David, Dhillon, Namrita, Kamakaka, Rohinton T.

The absolute requirement for the histone deacetylase activity of Sir2p in silencing coupled with the conservation of Sir2p-like proteins in larger eukaryotes suggests that this molecule plays an...

RNA polymerase III and RNA polymerase II promoter complexes are heterochromatin barriers in Saccharomyces cerevisiae

Donze, David, Kamakaka, Rohinton T.

The chromosomes of eukaryotes are organized into structurally and functionally discrete domains. Several DNA elements have been identified that act to separate these chromatin domains. We report a...

The boundaries of the silenced HMR domain in Saccharomyces cerevisiae

Donze, David, Adams, Christopher R., Rine, Jasper, Kamakaka, Rohinton T.

The chromosomes of eukaryotes are organized into structurally and functionally discrete domains that provide a mechanism to compact the DNA as well as delineate independent units of gene activity. It...

The Saccharomyces cerevisiae TRT2 tRNAThr gene upstream of STE6 is a barrier to repression in MATα cells and exerts a potential tRNA position effect in MATa cells

Simms, Tiffany A., Miller, Elsy C., Buisson, Nicolas P., Jambunathan, Nithya, Donze, David

A growing body of evidence suggests that genes transcribed by RNA polymerase III exhibit multiple functions within a chromosome. While the predominant function of these genes is the synthesis of RNA...

Modulation of Yeast Genome Expression in Response to Defective RNA Polymerase III-Dependent Transcription†

Conesa, Christine, Ruotolo, Roberta, Soularue, Pascal, Simms, Tiffany A., Donze, David, Sentenac, André, ...

We used genome-wide expression analysis in Saccharomyces cerevisiae to explore whether and how the expression of protein-coding, RNA polymerase (Pol) II-transcribed genes is influenced by a decrease...

Multiple Bromodomain Genes Are Involved in Restricting the Spread of Heterochromatic Silencing at the Saccharomyces cerevisiae HMR-tRNA Boundary

Jambunathan, Nithya, Martinez, Adam W., Robert, Elizabeth C., Agochukwu, Nneamaka B., Ibos, Megan E., Dugas, Sandra L., ...

The transfer RNA gene downstream from the HMR locus in S. cerevisiae functions as part of a boundary (barrier) element that restricts the spread of heterochromatic gene silencing into the downstream...

Characterization of a male-predominant antisense transcript underexpressed in hybrids of Drosophila pseudoobscura and D. persimilis.

Noor, Mohamed A F, Michalak, Pawel, Donze, David

Characterizing genes that are misregulated in hybrids may elucidate the genetic basis of hybrid sterility or other hybrid dysfunctions that contribute to speciation. Previously, a small segment of a...

Sir2p exists in two nucleosome-binding complexes with distinct deacetylase activities

Ghidelli, Sonja, Donze, David, Dhillon, Namrita, Kamakaka, Rohinton T.

The absolute requirement for the histone deacetylase activity of Sir2p in silencing coupled with the conservation of Sir2p-like proteins in larger eukaryotes suggests that this molecule plays an...

RNA polymerase III and RNA polymerase II promoter complexes are heterochromatin barriers in Saccharomyces cerevisiae

Donze, David, Kamakaka, Rohinton T.

The chromosomes of eukaryotes are organized into structurally and functionally discrete domains. Several DNA elements have been identified that act to separate these chromatin domains. We report a...

The boundaries of the silenced HMR domain in Saccharomyces cerevisiae

Donze, David, Adams, Christopher R., Rine, Jasper, Kamakaka, Rohinton T.

The chromosomes of eukaryotes are organized into structurally and functionally discrete domains that provide a mechanism to compact the DNA as well as delineate independent units of gene activity. It...

The Saccharomyces cerevisiae TRT2 tRNAThr gene upstream of STE6 is a barrier to repression in MATα cells and exerts a potential tRNA position effect in MATa cells

Simms, Tiffany A., Miller, Elsy C., Buisson, Nicolas P., Jambunathan, Nithya, Donze, David

A growing body of evidence suggests that genes transcribed by RNA polymerase III exhibit multiple functions within a chromosome. While the predominant function of these genes is the synthesis of RNA...

Modulation of Yeast Genome Expression in Response to Defective RNA Polymerase III-Dependent Transcription†

Conesa, Christine, Ruotolo, Roberta, Soularue, Pascal, Simms, Tiffany A., Donze, David, Sentenac, André, ...

We used genome-wide expression analysis in Saccharomyces cerevisiae to explore whether and how the expression of protein-coding, RNA polymerase (Pol) II-transcribed genes is influenced by a decrease...

Multiple Bromodomain Genes Are Involved in Restricting the Spread of Heterochromatic Silencing at the Saccharomyces cerevisiae HMR-tRNA Boundary

Jambunathan, Nithya, Martinez, Adam W., Robert, Elizabeth C., Agochukwu, Nneamaka B., Ibos, Megan E., Dugas, Sandra L., ...

The transfer RNA gene downstream from the HMR locus in S. cerevisiae functions as part of a boundary (barrier) element that restricts the spread of heterochromatic gene silencing into the downstream...

Characterization of a male-predominant antisense transcript underexpressed in hybrids of Drosophila pseudoobscura and D. persimilis.

Noor, Mohamed A F, Michalak, Pawel, Donze, David

Characterizing genes that are misregulated in hybrids may elucidate the genetic basis of hybrid sterility or other hybrid dysfunctions that contribute to speciation. Previously, a small segment of a...

Requirement of Nhp6 Proteins for Transcription of a Subset of tRNA Genes and Heterochromatin Barrier Function in Saccharomyces cerevisiae▿

Braglia, Priscilla, Dugas, Sandra L., Donze, David, Dieci, Giorgio

A key event in tRNA gene (tDNA) transcription by RNA polymerase (Pol) III is the TFIIIC-dependent assembly of TFIIIB upstream of the transcription start site. Different tDNA upstream sequences bind...