O'Connell, Ryan M., Rao, Dinesh S., Chaudhuri, Aadel A., Boldin, Mark P., Taganov, Konstnatin D., Nicoll, John, ...
Mammalian microRNAs are emerging as key regulators of the development and function of the immune system. Here, we report a strong but transient induction of miR-155 in mouse bone marrow after...
An, Dong Sung, Donahue, Robert E., Kamata, Masakazu, Poon, Betty, Metzger, Mark, Mao, Si-Hua, ...
RNAi is a powerful method for suppressing gene expression that has tremendous potential for therapeutic applications. However, because endogenous RNAi plays a role in normal cellular functions,...
MicroRNA-155 is induced during the macrophage inflammatory response (2007)
O'Connell, Ryan M., Taganov, Konstantin D., Boldin, Mark P., Cheng, Genhong, Baltimore, David
The mammalian inflammatory response to infection involves the induction of several hundred genes, a process that must be carefully regulated to achieve pathogen clearance and prevent the consequences...
Taganov, Konstantin D., Boldin, Mark P., Chang, Kuang-Jung, Baltimore, David
Activation of mammalian innate and acquired immune responses must be tightly regulated by elaborate mechanisms to control their onset and termination. MicroRNAs have been implicated as negative...
Targeting lentiviral vectors to specific cell types in vivo (2006)
Yang, Lili, Bailey, Leslie, Baltimore, David, Wang, Pin
We have developed an efficient method to target lentivirus-mediated gene transduction to a desired cell type. It involves incorporation of antibody and fusogenic protein as two distinct molecules...
Circuitry of nuclear factor κB signaling (2006)
Hoffmann, Alexander, Baltimore, David
Over the past few years, the transcription factor nuclear factor (NF)-κB and the proteins that regulate it have emerged as a signaling system of pre-eminent importance in human physiology and in an...
A method to genetically program mouse hematopoietic stem cells to develop into functional CD8 or CD4 T cells of defined specificity in vivo is described. For this purpose, a bicistronic retroviral...
Zarnegar, Brian, He, Jeannie Q., Oganesyan, Gagik, Hoffmann, Alexander, Baltimore, David, Cheng, Genhong
B lymphocytes can be activated by many different stimuli. However, the mechanisms responsible for the signaling and functional specificities of individual stimuli remain to be elucidated. Here, we...
Efficient Gene Targeting Mediated by Adeno-Associated Virus and DNA Double-Strand Breaks (2003)
Porteus, Matthew H., Cathomen, Toni, Weitzman, Matthew D., Baltimore, David
Gene targeting is the in situ manipulation of the sequence of an endogenous gene by the introduction of homologous exogenous DNA. Presently, the rate of gene targeting is too low for it to be broadly...
Qin, Xiao-Feng, An, Dong Sung, Chen, Irvin S. Y., Baltimore, David
Double-stranded RNAs approximate to 21 nucleotides long [small interfering RNA (siRNA)] are recognized as powerful reagents to reduce the expression of specific genes. To use them as reagents to...
Yang, Lili, Qin, Xiao-Feng, Baltimore, David, Van Parijs, Luk
We have developed an alternative to transgenesis for producing antigen-specific T cells in vivo. In this system, clonal naive T cells with defined antigen specificity are generated by...
Pohl, Thomas, Gugasyan, Raffi, Grumont, Raelene J., Strasser, Andreas, Metcalf, Donald, Tarlinton, David, ...
Transcription factors NF-KB1 and c-Rel, individually dispensable during embryogenesis, serve similar, yet distinct, roles in the function of mature hemopoietic cells. Redundancy among Rel/ NF-KB...
Cooperation of multiple signaling pathways in CD40-regulated gene expression in B lymphocytes (2002)
Dadgostar, Hajir, Zarnegar, Brian, Hoffmann, Alexander, Qin, Xiao-Feng, Truong, Uyen, Rao, Govinda, ...
CD40/CD40L interaction is essential for multiple biological events in T dependent humoral immune responses, including B cell survival and proliferation, germinal center and memory B cell formation,...
Alcamo, Elizabeth, Hacohen, Nir, Schulte, Leah C., Rennert, Paul D., Hynes, Richard O., Baltimore, David
The transcription factor nuclear factor (NF)-kappaB has been suggested to be a key mediator of the development of lymph nodes and Peyer's patches. However, targeted deletion of NF-kappaB/ Rel family...
Domain-dependent Function of the rasGAP-binding Protein p62Dok in Cell Signaling (2001)
Songyang, Zhou, Yamanashi, Yuji, Liu, Dan, Baltimore, David
p62Dok, the rasGAP-binding protein, is a common target of protein-tyrosine kinases. It is one of the major tyrosine-phosphorylated molecules in v-Src-transformed cells. Dok consists of an...
Severe B Cell Deficiency in Mice Lacking the Tec Kinase Family Members Tec and Btk (2000)
Ellmeier, Wilfried, Jung, Steffen, Sunshine, Mary Jean, Hatam, Farah, Xu, Yang, Baltimore, David, ...
Selective requirement for c-Rel during IL-12 P40 gene induction in macrophages (2000)
Sanjabi, Shomyseh, Hoffmann, Alexander, Liou, Hsiou-Chi, Baltimore, David, Smale, Stephen T.
A major challenge in the study of gene regulation by NF-kappaB/Rel transcription factors is to understand, at the biological and mechanistic levels, the selective functions of individual Rel family...
Cohen, George B., Rangan, Vangipuram S., Chen, Benjamin K., Smith, Stuart, Baltimore, David
A HIV-1 Nef affinity column was used to purify a 35-kDa Nef-interacting protein from T-cell lysates. The 35-kDa protein was identified by peptide microsequence analysis as the human thioesterase II...
V(D)J recombination is not activated by demethylation of the kappa locus (2000)
Cherry, Sara R., Beard, Caroline, Jaenisch, Rudolf, Baltimore, David
V(D)J recombination is thought to be regulated by changes in the accessibility of target sites, such as modulation of methylation. To test whether demethylation of the kappa locus can activate...
Grossmann, Mathis, Metcalf, Donald, Merryfull, Julie, Beg, Amer, Baltimore, David, Gerondakis, Steve
Individual Rel/NF-kappa B transcription factors, although dispensable for the development and maturation of most hemopoietic cells, are critical regulators of normal immune function. Redundancy among...
Chromatin remodeling directly activates V(D)J recombination (1999)
Cherry, Sara R., Baltimore, David
V(D)J recombination substrate choice is regulated to ensure that the appropriate gene segments are rearranged during lymphocyte development. It has been proposed that regulation of substrate usage is...
Radiation-induced Assembly of Rad51 and Rad52 Recombination Complex Requires ATM and c-Abl (1999)
Chen, Gang, Yuan, Shyng-Shiou F., Liu, Wei, Xu, Yang, Trujillo, Kelly, Song, Binwei, ...
Cells from individuals with the recessive cancer-prone disorder ataxia telangiectasia (A-T) are hypersensitive to ionizing radiation (I-R). ATM (mutated in A-T) is a protein kinase whose activity is...
Chang, Howard Y., Yang, Xiaolu, Baltimore, David
Fas is a cell surface death receptor that regulates peripheral tolerance and lymphoid homeostasis. In many pathologic conditions, ectopic Fas activation mediates tissue destruction. Several proteins...
Lee, Ho H., Dempsey, Paul W., Parks, Thomas P., Zhu, Xiaoqing, Baltimore, David, Cheng, Genhong
Several tumor necrosis factor receptor-associated factor (TRAF) family proteins including TRAF2, TRAF3, TRAF5, and TRAF6, as well as Jak3, have been implicated as potential mediators of CD40...
Involvement of p53 and p21 in Cellular Defects and Tumorigenesis in Atmminus /minus Mice (1998)
Xu, Yang, Yang, Eva Marie, Brugarolas, James, Jacks, Tyler, Baltimore, David
Disruption of the mouse Atm gene, whose human counterpart is consistently mutated in ataxia-telangiectasia (A-T) patients, creates an A-T mouse model exhibiting most of the A-T-related systematic and...
HIV-1 Directly Kills CD4+ T Cells by a Fas-independent Mechanism (1998)
Gandhi, Rajesh T., Chen, Benjamin K., Straus, Stephen E., Dale, Janet K., Lenardo, Michael J., Baltimore, David
The mechanism by which HIV-1 induces CD4+ T cell death is not known. A fundamental issue is whether HIV-1 primarily induces direct killing of infected cells or indirectly causes death of uninfected...
Kuljis, Rodrigo O., Xu, Yang, Aguila, M. Cecilia, Baltimore, David
Neural degeneration is one of the clinical manifestations of ataxia-telangiectasia, a disorder caused by mutations in the Atm protein kinase gene, However, neural degeneration was not detected with...
Interleukin 3-dependent survival by the Akt protein kinase (1997)
Songyang, Zhou, Baltimore, David, Cantley, Lewis C., Kaplan, David R., Franke, Thomas F.
Interleukin 3 (IL-3)-dependent survival of hematopoietic cells is known to rely on the activity of multiple signaling pathways, including a pathway leading to activation of phosphoinositide 3-kinase...
Chen, Benjamin K., Feinberg, Mark B., Baltimore, David
The dependence of human immunodeficiency virus type 1 (HIV-1) on its NF- kappaB binding sites (kappaB sites) for replication in transformed and primary T-cell targets was examined by infecting cells...
In vitro V(D)J recombination: Signal joint formation (1996)
Cortes, Patricia, Weis-Garcia, Frances, Misulovin, Ziva, Nussenzweig, Andre, Lai, Jiann-Shiun, Li, Gloria, ...
The first step of V(D)J recombination, specific cleavage at the recombination signal sequence (RSS), can be carried out by the recombination activating proteins RAG1 and RAG2. In vivo, the cleaved...
Chen, Benjamin K., Ghandi, Rajesh T., Baltimore, David
The human immunodeficiency virus type 1 (HIV-1) genes vpu, env, and nef have all been implicated in modulating the levels of cell surface CD4 on infected cells. To quantitatively assess the relative...
Rubin, Robert H., Baltimore, David, Chen, Benjamin K., Wilkinson, Robert A., Fischman, Alan J.
The tissue distribution of CD4 lymphocytes in normal C57/BL mice and CD4 knockout mice was determined by biodistribution measurements and gamma camera imaging with an In-111-labeled rat IgG(2b)...
Roman, Christopher A. J., Baltimore, David
RAG1 protein is essential for the activation of V(D)J recombination in developing lymphocytes (V, variable; D, diversity; J, joining). However, it has not been determined whether its role involves...
Xu, Yang, Davidson, Laurie, Alt, Frederick W., Baltimore, David
The pre-T-cell receptor, composed of the T-cell receptor (TCR) beta chain (TCR beta), pre-T alpha (pT alpha) chain, and CD3 molecules, has been postulated to be a transducer of signals during the...
Proline-Rich Sequences that Bind to Src Homology 3 Domains with Individual Specificities (1995)
Alexandropoulos, Konstantina, Cheng, Genhong, Baltimore, David
To study the binding specificity of Src homology 3 (SH3) domains, we have screened a mouse embryonic expression library for peptide fragments that interact with them. Several clones were identified...
Reduction of caveolin and caveolae in oncogenically transformed cells (1995)
Koleske, Anthony J., Baltimore, David, Lisanti, Michael P.
Caveolae are flask-shaped non-clathrin-coated invaginations of the plasma membrane. In addition to the demonstrated roles for caveolae in potocytosis and transcytosis, caveolae may regulate the...
Binding of Vav to Grb2 through dimerization of Src homology 3 domains (1994)
Ye, Zheng-Sheng, Baltimore, David
The protooncogenic protein Vav has the structure of an intracellular signal transducer. It is exclusively expressed in cells of hematopoietic lineage and plays a crucial role in hematopoietic cell...
Molecular evolution of the vertebrate immune system (1994)
Bartl, Simona, Baltimore, David, Weissman, Irving L.
An understanding of the evolution of vertebrate immunity is slowly emerging from studies of chordates that share distant ancestors with mammals. In higher vertebrates, such as birds and mammals, we...
Cheng, Genhong, Ze, Zheng-Sheng, Baltimore, David
Bruton's tyrosine kinase (Btk) is a recently described B-cell-specific tyrosine kinase. Mutations in this gene lead to human X chromosome-linked agammaglobulinemia and murine X-linked...
Cortes, Patricia, Ye, Zheng-Sheng, Baltimore, David
Genes for immunoglobulins and T-cell receptor are generated by a process known as V(D)J recombination. This process is highly regulated and mediated by the recombination activating proteins RAG-1 and...
Sequential induction of NF-kappa B/Rel family proteins during B-cell terminal differentiation (1994)
Liou, Hsiou-Chi, Sha, William C., Scott, Martin L., Baltimore, David
The NF-kappa B/Rel family of at least five transcription factor polypeptides is thought to function both as a developmental regulator in B cells and as a rapid response system in all cells. To...
Saksela, Kalle, Stevens, Cladd, Rubinstein, Pablo, Baltimore, David
To address the significance of human immunodeficiency virus (HIV) replication in peripheral blood mononuclear cells (PBMCs), we have used reverse transcriptase-initiated PCR to measure HIV-1 mRNA...
Van Etten, Richard A., Jackson, Peter K., Baltimore, David, Sanders, Mitchell C., Matsudaira, Paul T., Janmey, Paul A.
The myristoylated form of c-Abl protein, as well as the P210bcr/abl protein, have been shown by indirect immunofluorescence to associate with F-actin stress fibers in fibroblasts. Analysis of...
Chen, Benjamin K., Saksela, Kalle, Andino, Raul, Baltimore, David
To study the basis of cellular latency of human immunodeficiency virus (HIV), we have used a recombinant luciferase-encoding HIV (HXB-Luc) to superinfect nonproductively HIV-1-infected human leukemic...
Saksela, Kalle, Muchmore, Elizabeth, Girard, Marc, Fultz, Patricia, Baltimore, David
We have examined human immunodeficiency virus type 1 (HIV-1) infection in chimpanzees by analyzing HIV-1 DNA and RNA in lymph nodes and peripheral mononuclear cells (PBMCs). Like certain asymptomatic...
Nolan, Garry P., Fujita, Takashi, Bhatia, Kishor, Huppi, Conrad, Liou, Hsiou-Chi, Scott, Martin L., ...
The product of the putative proto-oncogene bcl-3 is an I kappa B-like molecule with novel binding properties specific for a subset of the rel family of transcriptional regulators. In vitro, Bcl-3...
Saksela, Kalle, Baltimore, David
B-cell-specific expression of the immunoglobulin kappa light-chain (Ig kappa) gene is in part accomplished by negative regulatory influences. Here we describe a new negatively acting element (termed...
Kamps, Mark P., Baltimore, David
One-quarter of pediatric pre-B-cell leukemias contain the t(1;19) chromosomal translocation, which fuses 5' exons encoding the transactivation domain of the E2A transcription factor gene to 3' exons...
Overduin, Michael, Mayer, Bruce, Rios, Carlos B., Baltimore, David, Cowburn, David
The Src homology 2 (SH2) domain is a recognition motif thought to mediate the association of the cytoplasmic proteins involved in signal transduction by binding to phosphotyrosyl-containing sequences...
Seshamma, Thikkavarapu, Bagasra, Omar, Trono, Didier, Baltimore, David, Pomerantz, Roger J.
Human immunodeficiency virus type 1 (HIV-1) infections of humans have a natural history characterized by a variable but usually slow progression to an immunodeficient state. We have described a...
Nonmyristoylated Abl proteins transform a factor-dependent hematopoietic cell line (1992)
Daley, George Q., Van Etten, Richard A., Jackson, Peter K., Bernards, Andre, Baltimore, David
N-terminal myristoylation can promote the association of proteins with the plasma membrane, a property that is required for oncogenic variants of Src and Abl to transform fibroblastic cell types. The...
Blast Crisis in a Murine Model of Chronic Myelogenous Leukemia (1991)
Daley, George Q., Van Etten, Richard A., Baltimore, David
The P210bcr/abl protein is produced in cells from patients with Philadelphia chromosome-positive chronic myelogenous leukemia (CML). Retroviral transfer of the gene encoding P210bcr/abl into murine...
v-abl causes hematopoietic disease distinct from that caused by bcr-abl (1991)
Scott, Martin L., Van Etten, Richard A., Daley, George Q., Baltimore, David
v-abl, the oncogene transduced by Abelson murine leukemia virus, was first characterized by its ability to transform lymphoid cells. bcr-abl, the oncogene formed by a t(9;22) translocation thought to...
Klotman, Mary E., Kim, Sunyoung, Buchbinder, Aby, DeRossi, Anita, Baltimore, David, Wong-Staal, Flossie
The genome of human immunodeficiency virus type 1 (HIV-1) encodes at least six proteins involved in regulation as well as the structural proteins Gag, Pol, and Env. The interplay of the various...
Feinberg, Mark B., Baltimore, David, Frankel, Alan D.
The mechanism of Tat transactivation was studied by treating cell lines containing Tat-defective viruses with purified Tat protein. These cell lines constitutively produce very low levels of virus in...
Mechanism of leukemogenesis induced by mink cell focus-forming murine leukemia viruses (1991)
The Friend or Moloney mink cell focus-forming (MCF) virus encodes a recombinant-type envelope glycoprotein, gp 70, that is closely related to the membrane glycoprotein, gp55, of Friend spleen...
Schlissel, Mark S., Corcoran, Lynn M., Baltimore, David
Virus-transformed pre-B cells undergo ordered immunoglobulin (Ig) gene rearrangements during culture. We devised a series of highly sensitive polymerase chain reaction assays for Ig gene...
Pomerantz, Roger J., Feinberg, Mark B., Andino, Raul, Baltimore, David
The long terminal repeats (LTRs) of human immunodeficiency virus type 1 (HIV-1) strains from the central nervous systems of four patients with AIDS and of an HIV-1 isolate which is highly...
B-cell- and myocyte-specific E2-box-binding factors contain E12/E47-like subunits (1991)
Murre, Cornelis, Voronova, Anna, Baltimore, David
Recent studies have identified a family of DNA-binding proteins that share a common DNA-binding and dimerization domain with the potential to form a helix-loop-helix (HLH) structure. Various HLH...
Mayer, Bruce J., Jackson, Peter K., Baltimore, David
Several proteins implicated in the regulation of cell proliferation contain a common noncatalytic domain, src homology region 2 (SH2). We have used the bacterially expressed SH2 domain of abl...
Selective expression of RAG-2 in chicken B cells undergoing immunoglobulin gene conversion (1991)
Carlson, Louise M., Oettinger, Marjorie A., Schatz, David G., Masteller, Emma L., Hurley, Elizabeth A., McCormack, Wayne T., ...
Chickens create their immunoglobulin (Ig) repertoires during B cell development in the bursa of Fabriclus by intrachromosomal gene conversion. Recent evidence has suggested that Ig gene conversion...
Factors affecting cellular tropism of human immunodeficiency virus (1990)
Kim, Sunyoung, Ikeuchi, Kenji, Groopman, Jerome, Baltimore, David
To evaluate the basis of the slow growth of many human immunodeficiency virus strains in monocytes/macrophages, various stages of the virus life cycle have been studied for their possible...
Kamps, Mark P., Corcoran, Lynn, LeBowitz, Jonathan H., Baltimore, David
The gene encoding interleukin-2 (IL-2) contains a sequence 52 to 326 nucleotides upstream of its transcriptional initiation site that promotes transcription in T cells that have been activated by...
Smale, Stephen T., Schmidt, Martin C., Berk, Arnold J., Baltimore, David
Transcription of mammalian genes by RNA polymerase II often begins at a specific nucleotide, whose location is determined either by an upstream DNA element known as a TATA box or by an element...
Andino, Raul, Rieckhof, Gabrielle E., Trono, Didier, Baltimore, David
The poliovirus mutant 5NC-11 has a 4-base insertion at position 70 within the 5' untranslated region and is deficient in RNA synthesis. Revertants from 5NC-11 were isolated, showing a partial...
Molecular cell biology / James Darnell, Harvey Lodish, David Baltimiore (1990)
Darnell, James, Lodish, Harvey, Baltimore, David
Incluye bibliografía e índice
Kim, Sunyoung, Byrn, Randal, Groopman, Jerome, Baltimore, David
The kinetics of retroviral DNA and RNA synthesis are parameters vital to understanding viral growth, especially for human immunodeficiency virus (HIV), which encodes several of its own regulatory...
Sun, Xiao-Hong, Baltimore, David
To study the effect of poliovirus protein 2A on cellular RNA translation, the tat control system of human immunodeficiency virus (HIV) was used. Protein 2A was expressed from a plasmid construct...
Daley, George Q., Baltimore, David
The P210bcr/abl protein is associated with virtually every case of human chronic myelogenous leukemia. Unlike the related P160gag/v-abl oncogene product of Abelson murine leukemia virus, P210bcr/abl...