Scientific Commons: Christopher M. Hylton

Characterization of Starch-Debranching Enzymes in Pea Embryos1

Zhu, Zhi-Ping, Hylton, Christopher M., Rössner, Ute, Smith, Alison M.

Two distinct types of debranching enzymes have been identified in developing pea (Pisum sativum L.) embryos using native gel analysis and tests of substrate preference on purified or partially...

The Altered Pattern of Amylose Accumulation in the Endosperm of Low-Amylose Barley Cultivars Is Attributable to a Single Mutant Allele of Granule-Bound Starch Synthase I with a Deletion in the 5′-Non-Coding Region1

Patron, Nicola J., Smith, Alison M., Fahy, Brendan F., Hylton, Christopher M., Naldrett, Mike J., Rossnagel, Brian G., ...

Reasons for the variable amylose content of endosperm starch from waxy cultivars of barley (Hordeum vulgare) were investigated. The mature grains of most such cultivars contain some amylose, although...

Starch granule initiation is controlled by a heteromultimeric isoamylase in potato tubers

Bustos, Regla, Fahy, Brendan, Hylton, Christopher M., Seale, Robert, Nebane, N. Miranda, Edwards, Anne, ...

Starch granule initiation is not understood, but recent evidence implicates a starch debranching enzyme, isoamylase, in the control of this process. Potato tubers contain isoamylase activity...

Interference by Phosphatases in the Spectrophotometric Assay for Phosphoenolpyruvate Carboxylase 1

Smith, Alison M., Hylton, Christopher M., Rawsthorne, Stephen

The aim of this work was to discover the extent of interference by phosphoenolpyruvate (PEP) phosphatase in spectrophotometric assays of PEP carboxylase (EC 4.1.1.31) in crude extracts of plant...

Spatial and Temporal Influences on the Cell-Specific Distribution of Glycine Decarboxylase in Leaves of Wheat (Triticum aestivum L.) and Pea (Pisum sativum L.) 1

Tobin, Alyson K., Thorpe, Julian R., Hylton, Christopher M., Rawsthorne, Stephen

The distribution of glycine decarboxylase (GDC) in leaves of pea (Pisum sativum L.) and wheat (Triticum aestivum L.) has been investigated using immunogold labeling of the P-protein subunit of the...