Christopher H. Contag

Assessment of Cellular Response to Thermal Laser Injury Through Bioluminescence Imaging of Heat Shock Protein 70¶† (2004)

Joshua T. Beckham, Mark A. Mackanos, Cornelia Crooke, Takamune Takahashi, Caitlin O'Connell-Rodwell, Christopher H. Contag, ...

Assessment of laser-induced tissue damage is not complete without an investigation into the resulting cellular and molecular changes. In the past, tissue damage was quantified macroscopically by...

Rapid Control of Wound Infections by Targeted Photodynamic Therapy Monitored by In Vivo Bioluminescence Imaging¶ (2002)

Michael R. Hamblin, David A. O'Donnell, Naveen Murthy, Christopher H. Contag, Tayyaba Hasan

The worldwide rise in antibiotic resistance necessitates the development of novel antimicrobial strategies. In this study we report on the first use of a photochemical approach to destroy bacteria...

Real-Time Monitoring of Escherichia coli O157:H7 Adherence to Beef Carcass Surface Tissues with a Bioluminescent Reporter (1999)

Siragusa, Gregory R., Nawotka, Kevin, Spilman, Stanley D., Contag, Pamela R., Contag, Christopher H.

A method for studying bacteria that are attached to carcass surfaces would eliminate the need for exogenous sampling and would facilitate understanding the interaction of potential human food-borne...

Visualizing the kinetics of tumor-cell clearance in living animals

Sweeney, Thomas J., Mailänder, Volker, Tucker, Amanda A., Olomu, Adesuwa B., Zhang, Weisheng, Cao, Yu-an, ...

Evaluation of potential antineoplastic therapies would be enhanced by noninvasive detection of tumor cells in living animals. Because light is transmitted through mammalian tissues, it was possible...

Real-Time Monitoring of Escherichia coli O157:H7 Adherence to Beef Carcass Surface Tissues with a Bioluminescent Reporter

Siragusa, Gregory R., Nawotka, Kevin, Spilman, Stanley D., Contag, Pamela R., Contag, Christopher H.

A method for studying bacteria that are attached to carcass surfaces would eliminate the need for exogenous sampling and would facilitate understanding the interaction of potential human food-borne...

Antigen-specific T cell–mediated gene therapy in collagen-induced arthritis

Nakajima, Atsuo, Seroogy, Christine M., Sandora, Matthew R., Tarner, Ingo H., Costa, Gina L., Taylor-Edwards, Cariel, ...

Autoantigen-specific T cells have tissue-specific homing properties, suggesting that these cells may be ideal vehicles for the local delivery of immunoregulatory molecules. We tested this hypothesis...

Shifting foci of hematopoiesis during reconstitution from single stem cells

Cao, Yu-An, Wagers, Amy J., Beilhack, Andreas, Dusich, Joan, Bachmann, Michael H., Negrin, Robert S., ...

To reveal the early events and dynamics of hematopoietic reconstitution in living animals in real-time, we used bioluminescence imaging to monitor engraftment from single luciferase-labeled...

The E47 transcription factor negatively regulates CD5 expression during thymocyte development

Yang, Yang, Contag, Christopher H., Felsher, Dean, Shachaf, Catherine M., Cao, Yuan, Herzenberg, Leonard A., ...

The expression of CD5 increases progressively as thymocytes mature. We have shown that CD5 expression is controlled by a tissue-specific regulatory promoter located upstream of the CD5 translation...

Hemin-activated macrophages home to the pancreas and protect from acute pancreatitis via heme oxygenase-1 induction

Nakamichi, Ikuo, Habtezion, Aida, Zhong, Bihui, Contag, Christopher H., Butcher, Eugene C., Omary, M. Bishr

Hemin upregulates heme oxygenase-1 (HO-1), a stress-induced enzyme implicated in protection from a variety of injuries while its related isoform HO-2 is constitutively expressed. The role of hemin or...

Induced Biliary Excretion of Listeria monocytogenes

Hardy, Jonathan, Margolis, Jeffrey J., Contag, Christopher H.

Listeria monocytogenes is a ubiquitous gram-positive bacterium that can cause systemic and often life-threatening disease in immunocompromised hosts. This organism is largely an intracellular...

Rapid and Quantitative Assessment of Cancer Treatment Response Using In Vivo Bioluminescence Imaging1

Rehemtulla, Alnawaz, Stegman, Lauren D, Cardozo, Shaun J, Gupta, Sheila, Hall, Daniel E, Contag, Christopher H, ...

Current assessment of orthotopic tumor models in animals utilizes survival as the primary therapeutic end point. In vivo bioluminescence imaging (BLI) is a sensitive imaging modality that is rapid...

Noninvasive Assessment of Tumor Cell Proliferation in Animal Models

Edinger, Matthias, Sweeney, Thomas J, Tucker, Amanda A, Olomu, Adesuwa B, Negrin, Robert S, Contag, Christopher H

Revealing the mechanisms of neoplastic disease and enhancing our ability to intervene in these processes requires an increased understanding of cellular and molecular changes as they occur in intact...

Use of Reporter Genes for Optical Measurements of Neoplastic Disease In vivo1

Contag, Christopher H, Jenkins, Darlene, Contag, Pamela R, Negrin, Robert S

Revealing the cellular and molecular changes associated with cancer, as they occur in intact living animal models of human neoplastic disease, holds tremendous potential for understanding disease...

Visualizing the kinetics of tumor-cell clearance in living animals

Sweeney, Thomas J., Mailänder, Volker, Tucker, Amanda A., Olomu, Adesuwa B., Zhang, Weisheng, Cao, Yu-an, ...

Evaluation of potential antineoplastic therapies would be enhanced by noninvasive detection of tumor cells in living animals. Because light is transmitted through mammalian tissues, it was possible...

Real-Time Monitoring of Escherichia coli O157:H7 Adherence to Beef Carcass Surface Tissues with a Bioluminescent Reporter

Siragusa, Gregory R., Nawotka, Kevin, Spilman, Stanley D., Contag, Pamela R., Contag, Christopher H.

A method for studying bacteria that are attached to carcass surfaces would eliminate the need for exogenous sampling and would facilitate understanding the interaction of potential human food-borne...

Antigen-specific T cell–mediated gene therapy in collagen-induced arthritis

Nakajima, Atsuo, Seroogy, Christine M., Sandora, Matthew R., Tarner, Ingo H., Costa, Gina L., Taylor-Edwards, Cariel, ...

Autoantigen-specific T cells have tissue-specific homing properties, suggesting that these cells may be ideal vehicles for the local delivery of immunoregulatory molecules. We tested this hypothesis...

Shifting foci of hematopoiesis during reconstitution from single stem cells

Cao, Yu-An, Wagers, Amy J., Beilhack, Andreas, Dusich, Joan, Bachmann, Michael H., Negrin, Robert S., ...

To reveal the early events and dynamics of hematopoietic reconstitution in living animals in real-time, we used bioluminescence imaging to monitor engraftment from single luciferase-labeled...

The E47 transcription factor negatively regulates CD5 expression during thymocyte development

Yang, Yang, Contag, Christopher H., Felsher, Dean, Shachaf, Catherine M., Cao, Yuan, Herzenberg, Leonard A., ...

The expression of CD5 increases progressively as thymocytes mature. We have shown that CD5 expression is controlled by a tissue-specific regulatory promoter located upstream of the CD5 translation...

Hemin-activated macrophages home to the pancreas and protect from acute pancreatitis via heme oxygenase-1 induction

Nakamichi, Ikuo, Habtezion, Aida, Zhong, Bihui, Contag, Christopher H., Butcher, Eugene C., Omary, M. Bishr

Hemin upregulates heme oxygenase-1 (HO-1), a stress-induced enzyme implicated in protection from a variety of injuries while its related isoform HO-2 is constitutively expressed. The role of hemin or...

Induced Biliary Excretion of Listeria monocytogenes

Hardy, Jonathan, Margolis, Jeffrey J., Contag, Christopher H.

Listeria monocytogenes is a ubiquitous gram-positive bacterium that can cause systemic and often life-threatening disease in immunocompromised hosts. This organism is largely an intracellular...

Rapid and Quantitative Assessment of Cancer Treatment Response Using In Vivo Bioluminescence Imaging1

Rehemtulla, Alnawaz, Stegman, Lauren D, Cardozo, Shaun J, Gupta, Sheila, Hall, Daniel E, Contag, Christopher H, ...

Current assessment of orthotopic tumor models in animals utilizes survival as the primary therapeutic end point. In vivo bioluminescence imaging (BLI) is a sensitive imaging modality that is rapid...

Noninvasive Assessment of Tumor Cell Proliferation in Animal Models

Edinger, Matthias, Sweeney, Thomas J, Tucker, Amanda A, Olomu, Adesuwa B, Negrin, Robert S, Contag, Christopher H

Revealing the mechanisms of neoplastic disease and enhancing our ability to intervene in these processes requires an increased understanding of cellular and molecular changes as they occur in intact...

Use of Reporter Genes for Optical Measurements of Neoplastic Disease In vivo1

Contag, Christopher H, Jenkins, Darlene, Contag, Pamela R, Negrin, Robert S

Revealing the cellular and molecular changes associated with cancer, as they occur in intact living animal models of human neoplastic disease, holds tremendous potential for understanding disease...

Ex Vivo Expanded Dendritic Cells Home to T-Cell Zones of Lymphoid Organs and Survive in Vivo after Allogeneic Bone Marrow Transplantation

Schimmelpfennig, Christoph H., Schulz, Stephan, Arber, Caroline, Baker, Jeanette, Tarner, Ingo, McBride, Jacqueline, ...

Little is known about adoptive transfer of allogeneic ex vivo expanded dendritic cells (eDCs). We investigated the trafficking pattern of eDCs in mice after allogeneic bone marrow transplantation by...

In vivo analyses of early events in acute graft-versus-host disease reveal sequential infiltration of T-cell subsets

Beilhack, Andreas, Schulz, Stephan, Baker, Jeanette, Beilhack, Georg F., Wieland, Courtney B., Herman, Edward I., ...

Graft-versus-host disease (GVHD) is a major obstacle in allogeneic hematopoietic cell transplantation. Given the dynamic changes in immune cell subsets and tissue organization, which occur in GVHD,...

Inhibition of CD4+CD25+ regulatory T-cell function by calcineurin-dependent interleukin-2 production

Zeiser, Robert, Nguyen, Vu H., Beilhack, Andreas, Buess, Martin, Schulz, Stephan, Baker, Jeanette, ...

CD4+CD25+ regulatory T (Treg) cells control immunologic tolerance and antitumor immune responses. Therefore, in vivo modification of Treg function by immunosuppressant drugs has broad implications...

Accelerated Bone Repair After Plasma Laser Corticotomies

Leucht, Philipp, Lam, Kentson, Kim, Jae-Beom, Mackanos, Mark A., Simanovskii, Dmitrii M., Longaker, Michael T., ...

To circumvent the untoward sequelae of thermal damage, we investigated the use of plasma lasers for corticotomies and uncovered a dramatic difference in the healing response. Plasma ablation created...

Real-time analysis of uptake and bioactivatable cleavage of luciferin-transporter conjugates in transgenic reporter mice

Wender, Paul A., Goun, Elena A., Jones, Lisa R., Pillow, Thomas H., Rothbard, Jonathan B., Shinde, Rajesh, ...

Many therapeutic leads fail to advance clinically because of bioavailability, selectivity, and formulation problems. Molecular transporters can be used to address these problems. Molecular...

Early CD30 signaling is critical for adoptively transferred CD4+CD25+ regulatory T cells in prevention of acute graft-versus-host disease

Zeiser, Robert, Nguyen, Vu H., Hou, Jing-Zhou, Beilhack, Andreas, Zambricki, Elizabeth, Buess, Martin, ...

Murine CD4+CD25+ regulatory T cells (Treg cells) reduce acute graft-versus-host disease (aGvHD). However, surface molecules critical for suppression are unclear. Deficiency of CD30 (CD30−/−)...

Detection of endogenous biomolecules in Barrett's esophagus by Fourier transform infrared spectroscopy

Wang, Thomas D., Triadafilopoulos, George, Crawford, James M., Dixon, Lisa R., Bhandari, Tarun, Sahbaie, Peyman, ...

Fourier transform infrared (FTIR) spectroscopy provides a unique molecular fingerprint of tissue from endogenous sources of light absorption; however, specific molecular components of the overall...