Guernier, Vanina, Sola, Christophe, Brudey, Karine, Guégan, Jean-François, Rastogi, Nalin
Abstract Background French Guiana has the highest tuberculosis (TB) burden among all French departments, with a strong increase in the TB incidence over the last few years. It is now uncertain how...
Kibiki, Gibson S, Mulder, Bert, Dolmans, Wil MV, De Beer, Jessica L, Boeree, Martin, Sam, Noel, ...
Abstract Background Tuberculosis (TB) is a major health problem and HIV is the major cause of the increase in TB. Sub-Saharan Africa is endemic for both TB and HIV infection. Determination of the...
Karboul, Anis, Gey Van Pittius, Nicolaas C, Namouchi, Amine, Vincent, Véronique, Sola, Christophe, Rastogi, Nalin, ...
Abstract Background The highly homologous PE_PGRS (Proline-glutamic acid_polymorphic GC-rich repetitive sequence) genes are members of the PE multigene family which is found only in mycobacteria. PE...
Aristimuño, Liselotte, Armengol, Raimond, Cebollada, Alberto, España, Mercedes, Guilarte, Alexis, Lafoz, Carmen, ...
Abstract Background Molecular typing of Mycobacterium tuberculosis strains has become a valuable tool in the epidemiology of tuberculosis (TB) by allowing detection of outbreaks, tracking of...
Brudey, Karine, Driscoll, Jeffrey R, Rigouts, Leen, Prodinger, Wolfgang M, Gori, Andrea, Al-Hajoj, Sahal A, ...
Abstract Background The Direct Repeat locus of the Mycobacterium tuberculosis complex (MTC) is a member of the CRISPR (Clustered regularly interspaced short palindromic repeats) sequences family....
Zozio, Thierry, Allix, Caroline, Gunal, Selami, Saribas, Zeynep, Alp, Alpaslan, Durmaz, Riza, ...
Abstract Background Population-based bacterial genetics using repeated DNA loci is an efficient approach to study the biodiversity and phylogeographical structure of human pathogens, such as...
Filliol, Ingrid, Sola, Christophe, Rastogi, Nalin
Spoligotyping, a method based on the variability of distribution of the 43 inter-direct repeat (DR) spacers of Mycobacterium tuberculosis and Mycobacterium bovis BCG, is useful to study the molecular...
Filliol, Ingrid, Ferdinand, Severine, Negroni, Laetitia, Sola, Christophe, Rastogi, Nalin
Fingerprinting based on variable numbers of tandem DNA repeats (VNTR), a recently described methodology, was evaluated for molecular typing of Mycobacterium tuberculosis in an insular setting. In...
Viana-Niero, Cristina, Gutierrez, Cristina, Sola, Christophe, Filliol, Ingrid, Boulahbal, Fadila, Vincent, Véronique, ...
A collection of 105 clinical isolates originally identified as Mycobacterium africanum were characterized using both phenotypic and genotyping methods. The phenotypic methods included routine...
Sola, Christophe, Ferdinand, Severine, Mammina, Caterina, Nastasi, Antonino, Rastogi, Nalin
In a previous study, we proposed to associate spoligotyping and typing with the variable number of tandem DNA repeats (VNTR) as an alternative strategy to IS6110-restriction fragment length...
Legrand, Eric, Filliol, Ingrid, Sola, Christophe, Rastogi, Nalin
Based on the variability of 43 spacers within the direct repeat (DR) locus of Mycobacterium tuberculosis complex organisms, spoligotyping is a rapid method that aids in the study of the epidemiology...
Goh, Khye Seng, Legrand, Eric, Sola, Christophe, Rastogi, Nalin
A total of 102 isolates of the Mycobacterium tuberculosis complex, including available “M. canettii” isolates, were studied by PCR-restriction analysis of a 441-bp fragment of the hsp65 gene. PRA...
Sola, Christophe, Horgen, Lionel, Maïsetti, Jerome, Devallois, Anne, Goh, Khye Seng, Rastogi, Nalin
A total of 129 clinical isolates of Mycobacterium tuberculosis representing 91 patients were typed by a combination of direct-repeat (DR)-based spoligotyping and an inter-IS6110–PGRS (polymorphic...
Characterization of Mycobacterium tuberculosis Complex DNAs from Egyptian Mummies by Spoligotyping
Zink, Albert R., Sola, Christophe, Reischl, Udo, Grabner, Waltraud, Rastogi, Nalin, Wolf, Hans, ...
Bone and soft tissue samples from 85 ancient Egyptian mummies were analyzed for the presence of ancient Mycobacterium tuberculosis complex DNA (aDNA) and further characterized by spoligotyping. The...
Ferdinand, Séverine, Sola, Christophe, Verdol, Béatrice, Legrand, Eric, Goh, Khye Seng, Berchel, Mylène, ...
Tuberculosis (TB) is one of the most common opportunistic diseases that appear among human immunodeficiency virus (HIV)-positive patients in Haiti. In this context the probable emergence of...
Sola, Christophe, Rastogi, Nalin, Gutierrez, M. Cristina, Vincent, Véronique, Brosch, Roland, Parsons, Linda
Evolutionary Relationships among Strains of Mycobacterium tuberculosis with Few Copies of IS6110
Dale, Jeremy W., Al-Ghusein, Hasan, Al-Hashmi, Salim, Butcher, Philip, Dickens, Anne L., Drobniewski, Francis, ...
Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a...
Characterization of Finnish Mycobacterium tuberculosis Isolates by Spoligotyping
Puustinen, Kirsi, Marjamäki, Merja, Rastogi, Nalin, Sola, Christophe, Filliol, Ingrid, Ruutu, Petri, ...
The molecular epidemiology of tuberculosis (TB) in Finland was studied by spoligotyping 380 Mycobacterium tuberculosis isolates. The isolates were obtained during a 1-year study period from July 2000...
Filliol, Ingrid, Driscoll, Jeffrey R., Van Soolingen, Dick, Kreiswirth, Barry N., Kremer, Kristin, Valétudie, Georges, ...
The present update on the global distribution of Mycobacterium tuberculosis complex spoligotypes provides both the octal and binary descriptions of the spoligotypes for M. tuberculosis complex,...
Niobe-Eyangoh, Sara Ngo, Kuaban, Christopher, Sorlin, Philippe, Cunin, Patrick, Thonnon, Jocelyn, Sola, Christophe, ...
We analyzed DNA polymorphisms in 455 Mycobacterium tuberculosis complex isolates from 455 patients to evaluate the biodiversity of tubercle bacilli in Ouest province, Cameroon. The phenotypic and...
Molecular Epidemiology of Mycobacterium tuberculosis in Western Sweden
Brudey, Karine, Gordon, Max, Moström, Peter, Svensson, Liselott, Jonsson, Bodil, Sola, Christophe, ...
The genetic diversity of Mycobacterium tuberculosis isolates among patients from Sweden was determined by a combination of two PCR-based techniques (spoligotyping and variable number of tandem...
Easterbrook, Philippa J., Gibson, Andrea, Murad, Shahed, Lamprecht, Dianie, Ives, Natalie, Ferguson, Alex, ...
We examined the pattern of tuberculosis (TB) transmission (i.e., reactivation versus recent transmission) and the impact of human immunodeficiency virus (HIV) infection in Harare, Zimbabwe....
Mycobacterium africanum Genotyping Using Novel Spacer Oligonucleotides in the Direct Repeat Locus
Brudey, Karine, Gutierrez, M. Cristina, Vincent, Véronique, Parsons, Linda M., Salfinger, Max, Rastogi, Nalin, ...
This study involves a first evaluation of 25 novel spacer oligonucleotides in addition to the 43 routine spacers for molecular characterization of a panel of 65 isolates of tubercle bacilli from...
Easterbrook*, Philippa J., Gibson, Andrea, Murad, Shahed, Lamprecht, Dianie, Ives, Natalie, Ferguson, Alex, ...
García De Viedma, Darío, Bouza, Emilio, Rastogi, Nalin, Sola, Christophe
In Spain, tuberculosis (TB) patterns are changing because of the recent increase in the number of cases among immigrants. To establish the composition of circulating Mycobacterium tuberculosis...
Lari, Nicoletta, Rindi, Laura, Sola, Christophe, Bonanni, Daniela, Rastogi, Nalin, Tortoli, Enrico, ...
Mycobacterium tuberculosis complex isolates (n = 248) collected during a 1-year period in Tuscany, Italy, were genotyped for the katG463 and gyrA95 polymorphisms and by standard spacer...
Goh, Khye Seng, Rastogi, Nalin, Berchel, Mylène, Huard, Richard C., Sola, Christophe
A well-characterized collection of Mycobacterium tuberculosis complex (MTC) isolates, representing all known subspecies as well as some relevant genotypic families of M. tuberculosis, was analyzed...
Nicol, Mark P., Sola, Christophe, February, Bradley, Rastogi, Nalin, Steyn, Lafras, Wilkinson, Robert J.
We studied the association between strain family and extrapulmonary tuberculosis among 285 children presenting to a pediatric hospital. Extrapulmonary disease occurred in 56% of children without...
Filliol, Ingrid, Motiwala, Alifiya S., Cavatore, Magali, Qi, Weihong, Hazbón, Manzour Hernando, Bobadilla Del Valle, Miriam, ...
We analyzed a global collection of Mycobacterium tuberculosis strains using 212 single nucleotide polymorphism (SNP) markers. SNP nucleotide diversity was high (average across all SNPs, 0.19), and...
Brudey, Karine, Filliol, Ingrid, Ferdinand, Séverine, Guernier, Vanina, Duval, Philippe, Maubert, Bertrand, ...
The three French overseas departments of the Americas are characterized both by insular (Guadeloupe and Martinique) and continental (French Guiana) settings with a tuberculosis case detection rate...
Brudey, Karine, Driscoll, Jeffrey R, Rigouts, Leen, Prodinger, Wolfgang M, Gori, Andrea, Al-Hajoj, Sahal A, ...
Filliol, Ingrid, Motiwala, Alifiya S., Cavatore, Magali, Qi, Weihong, Hazbón, Manzour Hernando, Bobadilla Del Valle, Miriam, ...
Aristimuño, Liselotte, Armengol, Raimond, Cebollada, Alberto, España, Mercedes, Guilarte, Alexis, Lafoz, Carmen, ...
Filliol, Ingrid, Sola, Christophe, Rastogi, Nalin
Spoligotyping, a method based on the variability of distribution of the 43 inter-direct repeat (DR) spacers of Mycobacterium tuberculosis and Mycobacterium bovis BCG, is useful to study the molecular...
Filliol, Ingrid, Ferdinand, Severine, Negroni, Laetitia, Sola, Christophe, Rastogi, Nalin
Fingerprinting based on variable numbers of tandem DNA repeats (VNTR), a recently described methodology, was evaluated for molecular typing of Mycobacterium tuberculosis in an insular setting. In...
Viana-Niero, Cristina, Gutierrez, Cristina, Sola, Christophe, Filliol, Ingrid, Boulahbal, Fadila, Vincent, Véronique, ...
A collection of 105 clinical isolates originally identified as Mycobacterium africanum were characterized using both phenotypic and genotyping methods. The phenotypic methods included routine...
Sola, Christophe, Ferdinand, Severine, Mammina, Caterina, Nastasi, Antonino, Rastogi, Nalin
In a previous study, we proposed to associate spoligotyping and typing with the variable number of tandem DNA repeats (VNTR) as an alternative strategy to IS6110-restriction fragment length...
Legrand, Eric, Filliol, Ingrid, Sola, Christophe, Rastogi, Nalin
Based on the variability of 43 spacers within the direct repeat (DR) locus of Mycobacterium tuberculosis complex organisms, spoligotyping is a rapid method that aids in the study of the epidemiology...
Goh, Khye Seng, Legrand, Eric, Sola, Christophe, Rastogi, Nalin
A total of 102 isolates of the Mycobacterium tuberculosis complex, including available “M. canettii” isolates, were studied by PCR-restriction analysis of a 441-bp fragment of the hsp65 gene. PRA...
Sola, Christophe, Horgen, Lionel, Maïsetti, Jerome, Devallois, Anne, Goh, Khye Seng, Rastogi, Nalin
A total of 129 clinical isolates of Mycobacterium tuberculosis representing 91 patients were typed by a combination of direct-repeat (DR)-based spoligotyping and an inter-IS6110–PGRS (polymorphic...
Characterization of Mycobacterium tuberculosis Complex DNAs from Egyptian Mummies by Spoligotyping
Zink, Albert R., Sola, Christophe, Reischl, Udo, Grabner, Waltraud, Rastogi, Nalin, Wolf, Hans, ...
Bone and soft tissue samples from 85 ancient Egyptian mummies were analyzed for the presence of ancient Mycobacterium tuberculosis complex DNA (aDNA) and further characterized by spoligotyping. The...
Ferdinand, Séverine, Sola, Christophe, Verdol, Béatrice, Legrand, Eric, Goh, Khye Seng, Berchel, Mylène, ...
Tuberculosis (TB) is one of the most common opportunistic diseases that appear among human immunodeficiency virus (HIV)-positive patients in Haiti. In this context the probable emergence of...
Sola, Christophe, Rastogi, Nalin, Gutierrez, M. Cristina, Vincent, Véronique, Brosch, Roland, Parsons, Linda
Evolutionary Relationships among Strains of Mycobacterium tuberculosis with Few Copies of IS6110
Dale, Jeremy W., Al-Ghusein, Hasan, Al-Hashmi, Salim, Butcher, Philip, Dickens, Anne L., Drobniewski, Francis, ...
Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a...
Characterization of Finnish Mycobacterium tuberculosis Isolates by Spoligotyping
Puustinen, Kirsi, Marjamäki, Merja, Rastogi, Nalin, Sola, Christophe, Filliol, Ingrid, Ruutu, Petri, ...
The molecular epidemiology of tuberculosis (TB) in Finland was studied by spoligotyping 380 Mycobacterium tuberculosis isolates. The isolates were obtained during a 1-year study period from July 2000...
Filliol, Ingrid, Driscoll, Jeffrey R., Van Soolingen, Dick, Kreiswirth, Barry N., Kremer, Kristin, Valétudie, Georges, ...
The present update on the global distribution of Mycobacterium tuberculosis complex spoligotypes provides both the octal and binary descriptions of the spoligotypes for M. tuberculosis complex,...
Niobe-Eyangoh, Sara Ngo, Kuaban, Christopher, Sorlin, Philippe, Cunin, Patrick, Thonnon, Jocelyn, Sola, Christophe, ...
We analyzed DNA polymorphisms in 455 Mycobacterium tuberculosis complex isolates from 455 patients to evaluate the biodiversity of tubercle bacilli in Ouest province, Cameroon. The phenotypic and...
Molecular Epidemiology of Mycobacterium tuberculosis in Western Sweden
Brudey, Karine, Gordon, Max, Moström, Peter, Svensson, Liselott, Jonsson, Bodil, Sola, Christophe, ...
The genetic diversity of Mycobacterium tuberculosis isolates among patients from Sweden was determined by a combination of two PCR-based techniques (spoligotyping and variable number of tandem...
Easterbrook, Philippa J., Gibson, Andrea, Murad, Shahed, Lamprecht, Dianie, Ives, Natalie, Ferguson, Alex, ...
We examined the pattern of tuberculosis (TB) transmission (i.e., reactivation versus recent transmission) and the impact of human immunodeficiency virus (HIV) infection in Harare, Zimbabwe....
Mycobacterium africanum Genotyping Using Novel Spacer Oligonucleotides in the Direct Repeat Locus
Brudey, Karine, Gutierrez, M. Cristina, Vincent, Véronique, Parsons, Linda M., Salfinger, Max, Rastogi, Nalin, ...
This study involves a first evaluation of 25 novel spacer oligonucleotides in addition to the 43 routine spacers for molecular characterization of a panel of 65 isolates of tubercle bacilli from...
Easterbrook*, Philippa J., Gibson, Andrea, Murad, Shahed, Lamprecht, Dianie, Ives, Natalie, Ferguson, Alex, ...
García De Viedma, Darío, Bouza, Emilio, Rastogi, Nalin, Sola, Christophe
In Spain, tuberculosis (TB) patterns are changing because of the recent increase in the number of cases among immigrants. To establish the composition of circulating Mycobacterium tuberculosis...
Lari, Nicoletta, Rindi, Laura, Sola, Christophe, Bonanni, Daniela, Rastogi, Nalin, Tortoli, Enrico, ...
Mycobacterium tuberculosis complex isolates (n = 248) collected during a 1-year period in Tuscany, Italy, were genotyped for the katG463 and gyrA95 polymorphisms and by standard spacer...
Goh, Khye Seng, Rastogi, Nalin, Berchel, Mylène, Huard, Richard C., Sola, Christophe
A well-characterized collection of Mycobacterium tuberculosis complex (MTC) isolates, representing all known subspecies as well as some relevant genotypic families of M. tuberculosis, was analyzed...
Nicol, Mark P., Sola, Christophe, February, Bradley, Rastogi, Nalin, Steyn, Lafras, Wilkinson, Robert J.
We studied the association between strain family and extrapulmonary tuberculosis among 285 children presenting to a pediatric hospital. Extrapulmonary disease occurred in 56% of children without...
Filliol, Ingrid, Motiwala, Alifiya S., Cavatore, Magali, Qi, Weihong, Hazbón, Manzour Hernando, Bobadilla Del Valle, Miriam, ...
We analyzed a global collection of Mycobacterium tuberculosis strains using 212 single nucleotide polymorphism (SNP) markers. SNP nucleotide diversity was high (average across all SNPs, 0.19), and...