Christophe Dez

Stable expression in yeast of the mature form of human telomerase RNA depends on its association with the box H/ACA small nucleolar RNP proteins Cbf5p, Nhp2p and Nop10p

Dez, Christophe, Henras, Anthony, Faucon, Bruno, Lafontaine, Denis L. J., Caizergues-Ferrer, Michèle, Henry, Yves

Telomerase is a ribonucleoprotein (RNP) particle required for the replication of telomeres. The RNA component, termed hTR, of human telomerase contains a domain structurally and functionally related...

Accumulation of H/ACA snoRNPs depends on the integrity of the conserved central domain of the RNA-binding protein Nhp2p

Henras, Anthony, Dez, Christophe, Noaillac-Depeyre, Jacqueline, Henry, Yves, Caizergues-Ferrer, Michèle

Box H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) play key roles in the synthesis of eukaryotic ribosomes. How box H/ACA snoRNPs are assembled remains unknown. Here we show that...

Snapshot of the Genome of the Pseudo-T-Even Bacteriophage RB49

Desplats, Carine, Dez, Christophe, Tétart, Françoise, Eleaume, Heïdy, Krisch, H. M.

RB49 is a virulent bacteriophage that infects Escherichia coli. Its virion morphology is indistinguishable from the well-known T-even phage T4, but DNA hybridization indicated that it was...

Naf1p, an Essential Nucleoplasmic Factor Specifically Required for Accumulation of Box H/ACA Small Nucleolar RNPs†

Dez, Christophe, Noaillac-Depeyre, Jacqueline, Caizergues-Ferrer, Michèle, Henry, Yves

Box H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) play key roles in the synthesis of eukaryotic ribosomes. The ways in which these particles are assembled and correctly localized...

Npa1p, a Component of Very Early Pre-60S Ribosomal Particles, Associates with a Subset of Small Nucleolar RNPs Required for Peptidyl Transferase Center Modification†

Dez, Christophe, Froment, Carine, Noaillac-Depeyre, Jacqueline, Monsarrat, Bernard, Caizergues-Ferrer, Michèle, Henry, Yves

We have identified a novel essential nucleolar factor required for the synthesis of 5.8S and 25S rRNAs termed Npa1p. In the absence of Npa1p, the pre-rRNA processing pathway leading to 5.8S and 25S...

Functional link between ribosome formation and biogenesis of iron–sulfur proteins

Yarunin, Alexander, Panse, Vikram Govind, Petfalski, Elisabeth, Dez, Christophe, Tollervey, David, Hurt, Ed

In genetic screens for ribosomal export mutants, we identified CFD1, NBP35 and NAR1 as factors involved in ribosome biogenesis. Notably, these components were recently reported to function in...

Yeast Ntr1/Spp382 Mediates Prp43 Function in Postspliceosomes

Boon, Kum-Loong, Auchynnikava, Tatsiana, Edwalds-Gilbert, Gretchen, Barrass, J. David, Droop, Alastair P., Dez, Christophe, ...

The Ntr1 and Ntr2 proteins of Saccharomyces cerevisiae have been reported to interact with proteins involved in pre-mRNA splicing, but their roles in the splicing process are unknown. We show here...

Stable expression in yeast of the mature form of human telomerase RNA depends on its association with the box H/ACA small nucleolar RNP proteins Cbf5p, Nhp2p and Nop10p

Dez, Christophe, Henras, Anthony, Faucon, Bruno, Lafontaine, Denis L. J., Caizergues-Ferrer, Michèle, Henry, Yves

Telomerase is a ribonucleoprotein (RNP) particle required for the replication of telomeres. The RNA component, termed hTR, of human telomerase contains a domain structurally and functionally related...

Accumulation of H/ACA snoRNPs depends on the integrity of the conserved central domain of the RNA-binding protein Nhp2p

Henras, Anthony, Dez, Christophe, Noaillac-Depeyre, Jacqueline, Henry, Yves, Caizergues-Ferrer, Michèle

Box H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) play key roles in the synthesis of eukaryotic ribosomes. How box H/ACA snoRNPs are assembled remains unknown. Here we show that...

Snapshot of the Genome of the Pseudo-T-Even Bacteriophage RB49

Desplats, Carine, Dez, Christophe, Tétart, Françoise, Eleaume, Heïdy, Krisch, H. M.

RB49 is a virulent bacteriophage that infects Escherichia coli. Its virion morphology is indistinguishable from the well-known T-even phage T4, but DNA hybridization indicated that it was...

Naf1p, an Essential Nucleoplasmic Factor Specifically Required for Accumulation of Box H/ACA Small Nucleolar RNPs†

Dez, Christophe, Noaillac-Depeyre, Jacqueline, Caizergues-Ferrer, Michèle, Henry, Yves

Box H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) play key roles in the synthesis of eukaryotic ribosomes. The ways in which these particles are assembled and correctly localized...

Npa1p, a Component of Very Early Pre-60S Ribosomal Particles, Associates with a Subset of Small Nucleolar RNPs Required for Peptidyl Transferase Center Modification†

Dez, Christophe, Froment, Carine, Noaillac-Depeyre, Jacqueline, Monsarrat, Bernard, Caizergues-Ferrer, Michèle, Henry, Yves

We have identified a novel essential nucleolar factor required for the synthesis of 5.8S and 25S rRNAs termed Npa1p. In the absence of Npa1p, the pre-rRNA processing pathway leading to 5.8S and 25S...

Functional link between ribosome formation and biogenesis of iron–sulfur proteins

Yarunin, Alexander, Panse, Vikram Govind, Petfalski, Elisabeth, Dez, Christophe, Tollervey, David, Hurt, Ed

In genetic screens for ribosomal export mutants, we identified CFD1, NBP35 and NAR1 as factors involved in ribosome biogenesis. Notably, these components were recently reported to function in...

Yeast Ntr1/Spp382 Mediates Prp43 Function in Postspliceosomes

Boon, Kum-Loong, Auchynnikava, Tatsiana, Edwalds-Gilbert, Gretchen, Barrass, J. David, Droop, Alastair P., Dez, Christophe, ...

The Ntr1 and Ntr2 proteins of Saccharomyces cerevisiae have been reported to interact with proteins involved in pre-mRNA splicing, but their roles in the splicing process are unknown. We show here...

Surveillance of nuclear-restricted pre-ribosomes within a subnucleolar region of Saccharomyces cerevisiae

Dez, Christophe, Houseley, Jonathan, Tollervey, David

We previously hypothesized that HEAT-repeat (Huntington, elongation A subunit, TOR) ribosome synthesis factors function in ribosome export. We report that the HEAT-repeat protein Sda1p is a component...

Characterization of Saccharomyces cerevisiae Npa2p (Urb2p) Reveals a Low-Molecular-Mass Complex Containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p Involved in Early Steps of 60S Ribosomal Subunit Biogenesis▿ †

Rosado, Iván V., Dez, Christophe, Lebaron, Simon, Caizergues-Ferrer, Michèle, Henry, Yves, De La Cruz, Jesús

We report the characterization of the yeast Npa2p (Urb2p) protein, which is essential for 60S ribosomal subunit biogenesis. We identified this protein in a synthetic lethal screening with the rsa3...

Roles of the HEAT repeat proteins Utp10 and Utp20 in 40S ribosome maturation

Dez, Christophe, Dlakić, Mensur, Tollervey, David

A family of HEAT-repeat containing ribosome synthesis factors was previously identified in Saccharomyces cerevisiae. We report the detailed characterization of two of these factors, Utp10 and Utp20,...