Christophe Carles

Is ribosome synthesis controlled by pol I transcription? (2008)

Chédin, Stéphane, Laferté, Arnaud, Hoang, Tran, Lafontaine, Denis L J, Riva, Michel, Carles, Christophe

Regulation of growth ultimately depends on the control of synthesis of new ribosomes. Ribosome biogenesis is thus a key element of cell biology, which is tightly regulated in response to...

Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I

Fath, Stephan, Milkereit, Philipp, Peyroche, Gerald, Riva, Michel, Carles, Christophe, Tschochner, Herbert

Regulation of rDNA transcription depends on the formation and dissociation of a functional complex between RNA polymerase I (pol I) and transcription initiation factor Rrn3p. We analyzed whether...

A Novel Subunit of Yeast RNA Polymerase III Interacts with the TFIIB-Related Domain of TFIIIB70

Ferri, Maria-Laura, Peyroche, Gérald, Siaut, Magali, Lefebvre, Olivier, Carles, Christophe, Conesa, Christine, ...

There is limited information on how eukaryotic RNA polymerases (Pol) recognize their cognate preinitiation complex. We have characterized a polypeptide copurifying with yeast Pol III. This protein,...

τ91, an Essential Subunit of Yeast Transcription Factor IIIC, Cooperates with τ138 in DNA Binding

Arrebola, Rosalía, Manaud, Nathalie, Rozenfeld, Sophie, Marsolier, Marie-Claude, Lefebvre, Olivier, Carles, Christophe, ...

Transcription factor IIIC (TFIIIC) (or τ) is a large multisubunit and multifunctional factor required for transcription of all class III genes in Saccharomyces cerevisiae. It is responsible for...

Localization of the yeast RNA polymerase I-specific subunits

Bischler, Nicolas, Brino, Laurent, Carles, Christophe, Riva, Michel, Tschochner, Herbert, Mallouh, Véronique, ...

The spatial distribution of four subunits specifically associated to the yeast DNA-dependent RNA polymerase I (RNA pol I) was studied by electron microscopy. A structural model of the native enzyme...

The A14–A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4–Rpb7 pol II subunits

Peyroche, Gérald, Levillain, Erwann, Siaut, Magali, Callebaut, Isabelle, Schultz, Patrick, Sentenac, André, ...

A43, an essential subunit of yeast RNA polymerase I (pol I), interacts with Rrn3, a class I general transcription factor required for rDNA transcription. The pol I–Rrn3 complex is the only form of...

The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3

Peyroche, Gérald, Milkereit, Philipp, Bischler, Nicolas, Tschochner, Herbert, Schultz, Patrick, Sentenac, André, ...

RNA polymerase I (Pol I) is dedicated to transcription of the large ribosomal DNA (rDNA). The mechanism of Pol I recruitment onto rDNA promoters is poorly understood. Here we present evidence that...

The RNA cleavage activity of RNA polymerase III is mediated by an essential TFIIS-like subunit and is important for transcription termination

Chédin, Stéphane, Riva, Michel, Schultz, Patrick, Sentenac, André, Carles, Christophe

Budding yeast RNA polymerase III (Pol III) contains a small, essential subunit, named C11, that is conserved in humans and shows a strong homology to TFIIS. A mutant Pol III, heterocomplemented with...

CTD kinase I is involved in RNA polymerase I transcription

Bouchoux, Céline, Hautbergue, Guillaume, Grenetier, Sabrina, Carles, Christophe, Riva, Michel, Goguel, Valérie

RNA polymerase II carboxy terminal domain (CTD) kinases are key elements in the control of mRNA synthesis. Yeast CTD kinase I (CTDK-I), is a non-essential complex involved in the regulation of mRNA...

Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I

Fath, Stephan, Milkereit, Philipp, Peyroche, Gerald, Riva, Michel, Carles, Christophe, Tschochner, Herbert

Regulation of rDNA transcription depends on the formation and dissociation of a functional complex between RNA polymerase I (pol I) and transcription initiation factor Rrn3p. We analyzed whether...

A Novel Subunit of Yeast RNA Polymerase III Interacts with the TFIIB-Related Domain of TFIIIB70

Ferri, Maria-Laura, Peyroche, Gérald, Siaut, Magali, Lefebvre, Olivier, Carles, Christophe, Conesa, Christine, ...

There is limited information on how eukaryotic RNA polymerases (Pol) recognize their cognate preinitiation complex. We have characterized a polypeptide copurifying with yeast Pol III. This protein,...

τ91, an Essential Subunit of Yeast Transcription Factor IIIC, Cooperates with τ138 in DNA Binding

Arrebola, Rosalía, Manaud, Nathalie, Rozenfeld, Sophie, Marsolier, Marie-Claude, Lefebvre, Olivier, Carles, Christophe, ...

Transcription factor IIIC (TFIIIC) (or τ) is a large multisubunit and multifunctional factor required for transcription of all class III genes in Saccharomyces cerevisiae. It is responsible for...

Localization of the yeast RNA polymerase I-specific subunits

Bischler, Nicolas, Brino, Laurent, Carles, Christophe, Riva, Michel, Tschochner, Herbert, Mallouh, Véronique, ...

The spatial distribution of four subunits specifically associated to the yeast DNA-dependent RNA polymerase I (RNA pol I) was studied by electron microscopy. A structural model of the native enzyme...

The A14–A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4–Rpb7 pol II subunits

Peyroche, Gérald, Levillain, Erwann, Siaut, Magali, Callebaut, Isabelle, Schultz, Patrick, Sentenac, André, ...

A43, an essential subunit of yeast RNA polymerase I (pol I), interacts with Rrn3, a class I general transcription factor required for rDNA transcription. The pol I–Rrn3 complex is the only form of...

The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3

Peyroche, Gérald, Milkereit, Philipp, Bischler, Nicolas, Tschochner, Herbert, Schultz, Patrick, Sentenac, André, ...

RNA polymerase I (Pol I) is dedicated to transcription of the large ribosomal DNA (rDNA). The mechanism of Pol I recruitment onto rDNA promoters is poorly understood. Here we present evidence that...

The RNA cleavage activity of RNA polymerase III is mediated by an essential TFIIS-like subunit and is important for transcription termination

Chédin, Stéphane, Riva, Michel, Schultz, Patrick, Sentenac, André, Carles, Christophe

Budding yeast RNA polymerase III (Pol III) contains a small, essential subunit, named C11, that is conserved in humans and shows a strong homology to TFIIS. A mutant Pol III, heterocomplemented with...

CTD kinase I is involved in RNA polymerase I transcription

Bouchoux, Céline, Hautbergue, Guillaume, Grenetier, Sabrina, Carles, Christophe, Riva, Michel, Goguel, Valérie

RNA polymerase II carboxy terminal domain (CTD) kinases are key elements in the control of mRNA synthesis. Yeast CTD kinase I (CTDK-I), is a non-essential complex involved in the regulation of mRNA...

A subcomplex of RNA polymerase III subunits involved in transcription termination and reinitiation

Landrieux, Emilie, Alic, Nazif, Ducrot, Cécile, Acker, Joël, Riva, Michel, Carles, Christophe

While initiation of transcription by RNA polymerase III (Pol III) has been thoroughly investigated, molecular mechanisms driving transcription termination remain poorly understood. Here we describe...

The transcriptional activity of RNA polymerase I is a key determinant for the level of all ribosome components

Laferté, Arnaud, Favry, Emmanuel, Sentenac, André, Riva, Michel, Carles, Christophe, Chédin, Stéphane

Regulation of ribosome biogenesis is a key element of cell biology, not only because ribosomes are directly required for growth, but also because ribosome production monopolizes nearly 80% of the...

Selectivity and proofreading both contribute significantly to the fidelity of RNA polymerase III transcription

Alic, Nazif, Ayoub, Nayla, Landrieux, Emilie, Favry, Emmanuel, Baudouin-Cornu, Peggy, Riva, Michel, ...

We examine here the mechanisms ensuring the fidelity of RNA synthesis by RNA polymerase III (Pol III). Misincorporation could only be observed by using variants of Pol III deficient in the intrinsic...