Vectors for co-expression of an unrestricted number of proteins (2007)
Scheich, Christoph, Kümmel, Daniel, Soumailakakis, Dimitri, Heinemann, Udo, Büssow, Konrad
A vector system is presented that allows generation of E. coli co-expression clones by a standardized, robust cloning procedure. The number of co-expressed proteins is not limited. Five ‘pQLink’...
Manjasetty, Babu A., Büssow, Konrad, Fieber-Erdmann, Martin, Roske, Yvette, Gobom, Johan, Scheich, Christoph, ...
The human protein PTD012 is the longer product of an alternatively spliced gene and was described to be localized in the nucleus. The X-ray structure analysis at 1.7 Å resolution of PTD012 through...
X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1) (2005)
Manjasetty, Babu A, Niesen, Frank H, Scheich, Christoph, Roske, Yvette, Goetz, Frank, Behlke, Joachim, ...
Abstract Background Human Aortic Preferentially Expressed Protein-1 (APEG-1) is a novel specific smooth muscle differentiation marker thought to play a role in the growth and differentiation of...
Büssow, Konrad, Scheich, Christoph, Sievert, Volker, Harttig, Ulrich, Schultz, Jörg, Simon, Bernd, ...
Abstract Background The availability of suitable recombinant protein is still a major bottleneck in protein structure analysis. The Protein Structure Factory, part of the international structural...
Büssow, Konrad, Scheich, Christoph, Sievert, Volker, Harttig, Ulrich, Schultz, Jörg, Simon, Bernd, ...
Background The availability of suitable recombinant protein is still a major bottleneck in protein structure analysis. The Protein Structure Factory, part of the international structural genomics...
X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1). (2005)
Manjasetty, Babu A, Niesen, Frank H, Scheich, Christoph, Roske, Yvette, Goetz, Frank, Behlke, Joachim, ...
BACKGROUND: Human Aortic Preferentially Expressed Protein-1 (APEG-1) is a novel specific smooth muscle differentiation marker thought to play a role in the growth and differentiation of arterial...
A catalog of human cDNA expression clones and its application to structural genomics (2004)
Büssow, Konrad, Quedenau, Claudia, Sievert, Volker, Tischer, Janett, Scheich, Christoph, Seitz, Harald, ...
Abstract We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli . A cDNA expression library...
A catalog of human cDNA expression clones and its application to structural genomics (2004)
Büssow,Konrad, Quedenau,Claudia, Sievert,Volker, Tischer,Janett, Scheich,Christoph, Seitz,Harald, ...
We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825...
Scheich, Christoph, Leitner, Dietmar, Sievert, Volker, Leidert, Martina, Schlegel, Brigitte, Simon, Bernd, ...
Abstract Background High-throughput protein structure analysis of individual protein domains requires analysis of large numbers of expression clones to identify suitable constructs for structure...
Scheich,Christoph, Leitner,Dietmar, Sievert,Volker, Leidert,Martina, Schlegel,Brigitte, Simon,Bernd, ...
Background High-throughput protein structure analysis of individual protein domains requires analysis of large numbers of expression clones to identify suitable constructs for structure...
An automated in vitro protein folding screen applied to a human dynactin subunit (2004)
Scheich,Christoph, Niesen,Frank H., Seckler,Robert, Büssow,Konrad
The preparation of proteins for structural and functional analysis using the Escherichia coli expression system is often hampered by the formation of insoluble intracellular protein aggregates...
Crystal structure of Homo sapiens protein hp14.5 (2004)
Manjasetty,Babu A., Delbrueck,Heinrich, Pham,Dinh-Trung, Mueller,Uwe, Fieber-Erdmann,Martin, Scheich,Christoph, ...
Das E. coli Expressionssystem ist das am häufigsten angewandte hinsichtlich der rekombinante Proteinexpression für strukturelle und funktionelle Analysen aufgrund der hohen erzielten Ausbeuten und...
Das E. coli Expressionssystem ist das am häufigsten angewandte hinsichtlich der rekombinante Proteinexpression für strukturelle und funktionelle Analysen aufgrund der hohen erzielten Ausbeuten und...
Das E. coli Expressionssystem ist das am häufigsten angewandte hinsichtlich der rekombinante Proteinexpression für strukturelle und funktionelle Analysen aufgrund der hohen erzielten Ausbeuten und...
Das E. coli Expressionssystem ist das am häufigsten angewandte hinsichtlich der rekombinante Proteinexpression für strukturelle und funktionelle Analysen aufgrund der hohen erzielten Ausbeuten und...
A catalog of human cDNA expression clones and its application to structural genomics (2004)
Büssow, Konrad, Quedenau, Claudia, Sievert, Volker, Tischer, Janett, Scheich, Christoph, Seitz, Harald, ...
We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825...
Crystal structure of Homo sapiens protein hp14.5 (2004)
Manjasetty, Babu A., Delbrueck, Heinrich, Pham, Dinh-Trung, Mueller, Uwe, Fieber-Erdmann, Martin, Scheich, Christoph, ...
Scheich, Christoph, Leitner, Dietmar, Sievert, Volker, Leidert, Martina, Schlegel, Brigitte, Simon, Bernd, ...
Background High-throughput protein structure analysis of individual protein domains requires analysis of large numbers of expression clones to identify suitable constructs for structure...
An automated in vitro protein folding screen applied to a human dynactin subunit (2004)
Scheich, Christoph, Niesen, Frank H., Seckler, Robert, Büssow, Konrad
The preparation of proteins for structural and functional analysis using the Escherichia coli expression system is often hampered by the formation of insoluble intracellular protein aggregates...
For recombinant production of proteins for structural and functional analyses, the E. coli expression system is the most widely used due to high yields and straightforward processing. However,...
Das E. coli Expressionssystem ist das am häufigsten angewandte hinsichtlich der rekombinante Proteinexpression für strukturelle und funktionelle Analysen aufgrund der hohen erzielten Ausbeuten und...
Scheich, Christoph, Sievert, Volker, Büssow, Konrad
Abstract Background Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods...
Scheich,Christoph, Sievert,Volker, Buessow,Konrad
Background Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods to express...
Scheich, Christoph, Sievert, Volker, Buessow, Konrad
Background Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods to express...
Scheich, Christoph, Leitner, Dietmar, Sievert, Volker, Leidert, Martina, Schlegel, Brigitte, Simon, Bernd, ...
A catalog of human cDNA expression clones and its application to structural genomics
Büssow, Konrad, Quedenau, Claudia, Sievert, Volker, Tischer, Janett, Scheich, Christoph, Seitz, Harald, ...
A systematic approach for identifying human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli is described.
Büssow, Konrad, Scheich, Christoph, Sievert, Volker, Harttig, Ulrich, Schultz, Jörg, Simon, Bernd, ...
X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1)
Manjasetty, Babu A, Niesen, Frank H, Scheich, Christoph, Roske, Yvette, Goetz, Frank, Behlke, Joachim, ...
Scheich, Christoph, Leitner, Dietmar, Sievert, Volker, Leidert, Martina, Schlegel, Brigitte, Simon, Bernd, ...
A catalog of human cDNA expression clones and its application to structural genomics
Büssow, Konrad, Quedenau, Claudia, Sievert, Volker, Tischer, Janett, Scheich, Christoph, Seitz, Harald, ...
A systematic approach for identifying human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli is described.
Büssow, Konrad, Scheich, Christoph, Sievert, Volker, Harttig, Ulrich, Schultz, Jörg, Simon, Bernd, ...
X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1)
Manjasetty, Babu A, Niesen, Frank H, Scheich, Christoph, Roske, Yvette, Goetz, Frank, Behlke, Joachim, ...
Vectors for co-expression of an unrestricted number of proteins
Scheich, Christoph, Kümmel, Daniel, Soumailakakis, Dimitri, Heinemann, Udo, Büssow, Konrad
A vector system is presented that allows generation of E. coli co-expression clones by a standardized, robust cloning procedure. The number of co-expressed proteins is not limited. Five ‘pQLink’...
Crystal structure of Homo sapiens PTD012 reveals a zinc-containing hydrolase fold
Manjasetty, Babu A., Büssow, Konrad, Fieber-Erdmann, Martin, Roske, Yvette, Gobom, Johan, Scheich, Christoph, ...
The human protein PTD012 is the longer product of an alternatively spliced gene and was described to be localized in the nucleus. The X-ray structure analysis at 1.7 Å resolution of PTD012 through...
An automated in vitro protein folding screen applied to a human dynactin subunit
Scheich, Christoph, Niesen, Frank H., Seckler, Robert, Büssow, Konrad
The preparation of proteins for structural and functional analysis using the Escherichia coli expression system is often hampered by the formation of insoluble intracellular protein aggregates...