Jeffrey A. Pleiss, Gregg B. Whitworth, Megan Bergkessel, Christine Guthrie
Appropriate expression of most eukaryotic genes requires the removal of introns from their pre–messenger RNAs (pre-mRNAs), a process catalyzed by the spliceosome. In higher eukaryotes a large...
The Saccharomyces cerevisiae RNA-binding protein Nab4/Hrp1 is a component of the cleavage factor complex required for 3′ pre-mRNA processing. Although the precise role of Nab4/Hrp1 remains unclear,...
The essential yeast RNA binding protein Npl3p is methylated
Siebel, Christian W., Guthrie, Christine
Arginine methylation is a prevalent modification found in many RNA binding proteins, yet little is known about its functional consequences. Using a monoclonal antibody, 1E4, we have shown that the...
Conservation in budding yeast of a kinase specific for SR splicing factors
Siebel, Christian W., Feng, Lana, Guthrie, Christine, Fu, Xiang-Dong
SR protein kinases (SRPKs) and their substrates, the SR family of serine/arginine-rich pre-mRNA splicing factors, appear to be key regulators of alternative splicing. Although SR proteins have been...
An essential nuclear envelope integral membrane protein, Brr6p, required for nuclear transport
Kops, Anne De Bruyn, Guthrie, Christine
Despite rapid advances in our understanding of the function of the nuclear pore complex in nuclear transport, little is known about the role the nuclear envelope itself may play in this critical...
Guthrie, Christine, Nashimoto, Hiroko, Nomura, Masayasu
E. coli mutants that fail to grow at 20°C were isolated, and three such mutants were studied in detail. Two of these are defective in 50S ribosome biosynthesis at low temperatures and accumulate 43S...
Alternative 3′-end processing of U5 snRNA by RNase III
Chanfreau, Guillaume, Elela, Sherif Abou, Ares, Manuel, Guthrie, Christine
The cellular components required to form the 3′ ends of small nuclear RNAs are unknown. U5 snRNA from Saccharomyces cerevisiae is found in two forms that differ in length at their 3′ ends (U5L...
Collins, Catherine A., Guthrie, Christine
The highly conserved spliceosomal protein Prp8 is known to cross-link the critical sequences at both the 5′ (GU) and 3′ (YAG) ends of the intron. We have identified prp8 mutants with the...
Inada, Maki, Guthrie, Christine
La is a conserved eukaryotic RNA-binding protein best known for its role in the biogenesis of noncoding RNAs transcribed by RNA polymerase III. To broaden our understanding of the function of the La...
Barrell, B. G., Seidman, J. G., Guthrie, Christine, McClain, William H.
The nucleotide sequence of a transfer RNA precursor molecule coded by bacteriophage T4 has been determined. The molecule is a single polynucleotide chain which contains two transfer RNA species that...
Eight Transfer RNAs Induced by Infection of Escherichia coli with Bacteriophage T4
McClain, William H., Guthrie, Christine, Barrell, B. G.
Bacteriophage T4 induces the synthesis of eight transfer RNAs upon infection of E. coli. The tRNAs are easily detected and resolved into pure species by polyacrylamide gel electrophoresis of RNA...
Villa, Tommaso, Guthrie, Christine
Prp16p is a DEAH-box ATPase that transiently associates with the spliceosome to promote the structural transition required for the second chemical step. Yeast strains carrying the cold-sensitive...
Preker, Pascal J, Kim, Karen S, Guthrie, Christine
Recent evidence supports the idea that pre-mRNA splicing and mRNA export are mechanistically coupled. In metazoans, this process appears to be mediated by a multicomponent complex, which associates...
A conserved Lsm-interaction motif in Prp24 required for efficient U4/U6 di-snRNP formation.
Rader, Stephen D, Guthrie, Christine
The assembly of the U4 and U6 snRNPs into the U4/U6 di-snRNP is necessary for pre-mRNA splicing, and in Saccharomyces cerevisiae requires the splicing factor Prp24. We have identified a family of...
KIM GUISBERT, KAREN, DUNCAN, KENT, LI, HAO, GUTHRIE, CHRISTINE
Nab2, Npl3, and Nab4/Hrp1 are essential RNA binding proteins of the shuttling hnRNP class that are required for the efficient export of mRNA. To characterize the in vivo transcript specificity of...
Kistler, Amy L., Guthrie, Christine
Mammalian U2AF65 and UAP56 are required for prespliceosome (PS) formation. We tested the predictions that the yeast UAP56 homolog, SUB2, is required for the same step and functions collaboratively...
Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p
BELLARE, PRIYA, KUTACH, ALAN K., RINES, AMY K., GUTHRIE, CHRISTINE, SONTHEIMER, ERIK J.
The U1, U2, U4/U6, and U5 small nuclear ribonucleoproteins (snRNPs) are components of the spliceosome, which catalyzes pre-mRNA splicing. One of the largest and the most highly conserved proteins in...
Brenner, Tamara J., Guthrie, Christine
Snu114 is the only GTPase required for mRNA splicing. As a homolog of elongation factor G, it contains three domains (III–V) predicted to undergo a large rearrangement following GTP hydrolysis. To...
Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain
Brenner, Tamara J., Guthrie, Christine
Snu114 is a U5 snRNP protein essential for pre-mRNA splicing. Based on its homology with the ribosomal translocase EF-G, it is thought that GTP hydrolysis by Snu114 induces conformational...
Autoregulation of the mRNA export factor Yra1p requires inefficient splicing of its pre-mRNA
Preker, Pascal J., Guthrie, Christine
Yra1p is an essential RNA-binding protein that couples transcription to export. The YRA1 gene is one of only ∼5% of genes that undergo splicing in budding yeast, and its intron is unusual in...
The essential yeast RNA binding protein Npl3p is methylated
Siebel, Christian W., Guthrie, Christine
Arginine methylation is a prevalent modification found in many RNA binding proteins, yet little is known about its functional consequences. Using a monoclonal antibody, 1E4, we have shown that the...
Conservation in budding yeast of a kinase specific for SR splicing factors
Siebel, Christian W., Feng, Lana, Guthrie, Christine, Fu, Xiang-Dong
SR protein kinases (SRPKs) and their substrates, the SR family of serine/arginine-rich pre-mRNA splicing factors, appear to be key regulators of alternative splicing. Although SR proteins have been...
An essential nuclear envelope integral membrane protein, Brr6p, required for nuclear transport
Kops, Anne De Bruyn, Guthrie, Christine
Despite rapid advances in our understanding of the function of the nuclear pore complex in nuclear transport, little is known about the role the nuclear envelope itself may play in this critical...
Guthrie, Christine, Nashimoto, Hiroko, Nomura, Masayasu
E. coli mutants that fail to grow at 20°C were isolated, and three such mutants were studied in detail. Two of these are defective in 50S ribosome biosynthesis at low temperatures and accumulate 43S...
Kistler, Amy L., Guthrie, Christine
Mammalian U2AF65 and UAP56 are required for prespliceosome (PS) formation. We tested the predictions that the yeast UAP56 homolog, SUB2, is required for the same step and functions collaboratively...
Alternative 3′-end processing of U5 snRNA by RNase III
Chanfreau, Guillaume, Elela, Sherif Abou, Ares, Manuel, Guthrie, Christine
The cellular components required to form the 3′ ends of small nuclear RNAs are unknown. U5 snRNA from Saccharomyces cerevisiae is found in two forms that differ in length at their 3′ ends (U5L...
Collins, Catherine A., Guthrie, Christine
The highly conserved spliceosomal protein Prp8 is known to cross-link the critical sequences at both the 5′ (GU) and 3′ (YAG) ends of the intron. We have identified prp8 mutants with the...
Inada, Maki, Guthrie, Christine
La is a conserved eukaryotic RNA-binding protein best known for its role in the biogenesis of noncoding RNAs transcribed by RNA polymerase III. To broaden our understanding of the function of the La...
Barrell, B. G., Seidman, J. G., Guthrie, Christine, McClain, William H.
The nucleotide sequence of a transfer RNA precursor molecule coded by bacteriophage T4 has been determined. The molecule is a single polynucleotide chain which contains two transfer RNA species that...
Eight Transfer RNAs Induced by Infection of Escherichia coli with Bacteriophage T4
McClain, William H., Guthrie, Christine, Barrell, B. G.
Bacteriophage T4 induces the synthesis of eight transfer RNAs upon infection of E. coli. The tRNAs are easily detected and resolved into pure species by polyacrylamide gel electrophoresis of RNA...
Villa, Tommaso, Guthrie, Christine
Prp16p is a DEAH-box ATPase that transiently associates with the spliceosome to promote the structural transition required for the second chemical step. Yeast strains carrying the cold-sensitive...
Preker, Pascal J, Kim, Karen S, Guthrie, Christine
Recent evidence supports the idea that pre-mRNA splicing and mRNA export are mechanistically coupled. In metazoans, this process appears to be mediated by a multicomponent complex, which associates...
A conserved Lsm-interaction motif in Prp24 required for efficient U4/U6 di-snRNP formation.
Rader, Stephen D, Guthrie, Christine
The assembly of the U4 and U6 snRNPs into the U4/U6 di-snRNP is necessary for pre-mRNA splicing, and in Saccharomyces cerevisiae requires the splicing factor Prp24. We have identified a family of...
KIM GUISBERT, KAREN, DUNCAN, KENT, LI, HAO, GUTHRIE, CHRISTINE
Nab2, Npl3, and Nab4/Hrp1 are essential RNA binding proteins of the shuttling hnRNP class that are required for the efficient export of mRNA. To characterize the in vivo transcript specificity of...
Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p
BELLARE, PRIYA, KUTACH, ALAN K., RINES, AMY K., GUTHRIE, CHRISTINE, SONTHEIMER, ERIK J.
The U1, U2, U4/U6, and U5 small nuclear ribonucleoproteins (snRNPs) are components of the spliceosome, which catalyzes pre-mRNA splicing. One of the largest and the most highly conserved proteins in...
Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain
Brenner, Tamara J., Guthrie, Christine
Snu114 is a U5 snRNP protein essential for pre-mRNA splicing. Based on its homology with the ribosomal translocase EF-G, it is thought that GTP hydrolysis by Snu114 induces conformational...
Brenner, Tamara J., Guthrie, Christine
Snu114 is the only GTPase required for mRNA splicing. As a homolog of elongation factor G, it contains three domains (III–V) predicted to undergo a large rearrangement following GTP hydrolysis. To...
Autoregulation of the mRNA export factor Yra1p requires inefficient splicing of its pre-mRNA
Preker, Pascal J., Guthrie, Christine
Yra1p is an essential RNA-binding protein that couples transcription to export. The YRA1 gene is one of only ∼5% of genes that undergo splicing in budding yeast, and its intron is unusual in...
Alternative 3′ Pre-mRNA Processing in Saccharomyces cerevisiae Is Modulated by Nab4/Hrp1 In Vivo
Kim Guisbert, Karen S., Li, Hao, Guthrie, Christine
The Saccharomyces cerevisiae RNA-binding protein Nab4/Hrp1 is a component of the cleavage factor complex required for 3′ pre-mRNA processing. Although the precise role of Nab4/Hrp1 remains unclear,...
Pleiss, Jeffrey A, Whitworth, Gregg B, Bergkessel, Megan, Guthrie, Christine
Appropriate expression of most eukaryotic genes requires the removal of introns from their pre–messenger RNAs (pre-mRNAs), a process catalyzed by the spliceosome. In higher eukaryotes a large...
Mitrovich, Quinn M., Tuch, Brian B., Guthrie, Christine, Johnson, Alexander D.
Candida albicans is the most common fungal pathogen of humans. Frequently found as a commensal within the digestive tracts of healthy individuals, C. albicans is an opportunistic pathogen that causes...
Evolution of small nuclear RNAs in S. cerevisiae, C. albicans, and other hemiascomycetous yeasts
Mitrovich, Quinn M., Guthrie, Christine
The spliceosome is a large, dynamic ribonuclear protein complex, required for the removal of intron sequences from newly synthesized eukaryotic RNAs. The spliceosome contains five essential small...
Lund, Mette K., Kress, Tracy L., Guthrie, Christine
Npl3 is an SR-like protein with documented roles in mRNA export and transcription termination. Maintaining appropriate levels of Npl3 protein is critical for cell survival. Here we show that Npl3...