Nadia Bakkour, Yea-Lih Lin, Sophie Maire, Lilia Ayadi, Florence Mahuteau-Betzer, Chi Hung Nguyen, ...
The development of multidrug-resistant viruses compromises antiretroviral therapy efficacy and limits therapeutic options. Therefore, it is an ongoing task to identify new targets for antiretroviral...
Recherche des gènes d'ARN non codant (2006)
Gothié, Emmanuel, Guermeur, Yann, Muller, Sébastien, Branlant, Christiane, Bockmayr, Alexander
La masse considérable de données brutes extraite des programmes de séquençage nécessite de nouvelles techniques d'analyse. La première étape visant à annoter les séquences génomiques est la...
A Multi-Site Constraint Programming Model of Alternative Splicing Regulation (2006)
Eveillard, Damien, Ropers, Delphine, De Jong, Hidde, Branlant, Christiane, Bockmayr, Alexander
Alternative splicing is a key process in post-transcriptional regulation, by which several kinds of mature RNA can be obtained from the same premessenger RNA. The resulting combinatorial complexity...
A Multi-Site Constraint Programming Model of Alternative Splicing Regulation (2006)
Eveillard, Damien, Ropers, Delphine, De Jong, Hidde, Branlant, Christiane, Bockmayr, Alexander
Alternative splicing is a key process in post-transcriptional regulation, by which several kinds of mature RNA can be obtained from the same premessenger RNA. The resulting combinatorial complexity...
Recherche des gènes d'ARN non codant (2006)
Gothié, Emmanuel, Guermeur, Yann, Muller, Sébastien, Branlant, Christiane, Bockmayr, Alexander
La masse considérable de données brutes extraite des programmes de séquençage nécessite de nouvelles techniques d'analyse. La première étape visant à annoter les séquences génomiques est la...
A multi-scale constraint programming model of alternative splicing regulation (2004)
Eveillard, Damien, Ropers, Delphine, De Jong, Hidde, Branlant, Christiane, Bockmayr, Alexander
Alternative splicing is a key process in post-transcriptional regulation, by which different mature RNA can be obtained from the same premessenger RNA. The resulting combinatorial complexity...
A multi-scale constraint programming model of alternative splicing regulation (2004)
Eveillard, Damien, Ropers, Delphine, De Jong, Hidde, Branlant, Christiane, Bockmayr, Alexander
Alternative splicing is a key process in post-transcriptional regulation, by which different mature RNA can be obtained from the same premessenger RNA. The resulting combinatorial complexity...
Recherche des gènes d'ARN non codant (2003)
Gothié, Emmanuel, Guermeur, Yann, Muller, Sébastien, Branlant, Christiane, Bockmayr, Alexander
La masse considérable de données brutes extraite des programmes de séquençage nécessite de nouvelles techniques d'analyse. La première étape visant à annoter les séquences génomiques est la...
A Multi-Site Constraint Programming Model of Alternative Splicing Regulation (2003)
Eveillard, Damien, Ropers, Delphine, De Jong, Hidde, Branlant, Christiane, Bockmayr, Alexander
Alternative splicing is a key process in post-transcriptional regulation, by which several kinds of mature RNA can be obtained from the same premessenger RNA. The resulting combinatorial complexity...
Multiscale Modeling of Alternative Splicing (2002)
Damien Eveillard, Christiane Branlant
Alternative splicing is a key process in post-transcriptional regulation, by which several kinds of mature RNA can be obtained from the same premessenger RNA. Using a constraint programming approach,...
Jacquenet, Sandrine, Ropers, Delphine, Bilodeau, Patricia S., Damier, Laurence, Mougin, Annie, Stoltzfus, C. Martin, ...
The HIV-1 transcript is alternatively spliced to over 30 different mRNAs. Whether RNA secondary structure can influence HIV-1 RNA alternative splicing has not previously been examined. Here we have...
Massenet, Séverine, Motorin, Yuri, Lafontaine, Denis L. J., Hurt, Eduard C., Grosjean, Henri, Branlant, Christiane
Pseudouridine (Ψ) residues were localized in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (UsnRNAs) by using the chemical mapping method. In contrast to vertebrate UsnRNAs, S....
Ségault, Véronique, Will, Cindy L., Polycarpou-Schwarz, Maria, Mattaj, Iain W., Branlant, Christiane, Lührmann, Reinhard
The function of conserved regions of the metazoan U5 snRNA was investigated by reconstituting U5 small nuclear ribonucleoprotein particles (snRNPs) from purified snRNP proteins and HeLa or Xenopus U5...
RNomics in Archaea reveals a further link between splicing of archaeal introns and rRNA processing
Tang, Thean Hock, Rozhdestvensky, Timofey S., D’Orval, Béatrice Clouet, Bortolin, Marie-Line, Huber, Harald, Charpentier, Bruno, ...
The bulge–helix–bulge (BHB) motif recognised by the archaeal splicing endonuclease is also found in the long processing stems of archaeal rRNA precursors in which it is cleaved to generate...
Branlant, Christiane, Krol, Alain, Machatt, Mohamed Ali, Pouyet, Jean, Ebel, Jean-Pierre, Edwards, Kaylene, ...
We determined 90% of the primary structure of E.coli MRE 600 23S rRNA by applying the sequencing gel technique to products of T1, S1, A and Naja oxiana nuclease digestion. Eight cistron...
Location of Ribosomal Protein Binding Sites on 16S Ribosomal RNA
Zimmermann, Robert A., Muto, Akira, Fellner, Peter, Ehresmann, Chantal, Branlant, Christiane
The distribution of ribosomal protein binding sites on the 16S ribosomal RNA molecule has been analyzed by limited ribonuclease hydrolysis of RNA-protein complexes, as well as by the interaction of...
Thouvenot, Benoit, Charpentier, Bruno, Branlant, Christiane
The Escherichia coli multi-promoter region of the gapA gene ensures a high level of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) production under various growth conditions. In the exponential...
Reconstitution of archaeal H/ACA small ribonucleoprotein complexes active in pseudouridylation
Charpentier, Bruno, Muller, Sébastien, Branlant, Christiane
Pseudouridine (Ψ) are frequently modified residues in RNA. In Eukarya, their formation is catalyzed by enzymes or by ribonucleoprotein complexes (RNPs) containing H/ACA snoRNAs. H/ACA sRNA and...
BEHM-ANSMANT, ISABELLE, URBAN, ALAN, MA, XIAOJU, YU, YI-TAO, MOTORIN, YURI, BRANLANT, CHRISTIANE
The Saccharomyces cerevisiae Pus7 protein was recently characterized as a novel RNA:pseudouridine (Ψ)-synthase acting at position 35 in U2 snRNA. However, U2 snRNA was the only potential substrate...
Manival, Xavier, Charron, Christophe, Fourmann, Jean-Baptiste, Godard, François, Charpentier, Bruno, Branlant, Christiane
In archaeal rRNAs, the isomerization of uridine into pseudouridine (Ψ) is achieved by the H/ACA sRNPs and the minimal set of proteins required for RNA:Ψ-synthase activity is the aCBF5–aNOP10...
MARMIER-GOURRIER, NATHALIE, CLÉRY, ANTOINE, SENTY-SÉGAULT, VÉRONIQUE, CHARPENTIER, BRUNO, SCHLOTTER, FLORENCE, LECLERC, FABRICE, ...
The 15.5-kD protein and its yeast homolog Snu13p bind U4 snRNA, U3 snoRNA, and the C/D box snoRNAs. In U4 snRNA, they associate with a helix-bulge-helix (K-turn) structure. U3 snoRNA contains two...
Jacquenet, Sandrine, Ropers, Delphine, Bilodeau, Patricia S., Damier, Laurence, Mougin, Annie, Stoltzfus, C. Martin, ...
The HIV-1 transcript is alternatively spliced to over 30 different mRNAs. Whether RNA secondary structure can influence HIV-1 RNA alternative splicing has not previously been examined. Here we have...
Massenet, Séverine, Motorin, Yuri, Lafontaine, Denis L. J., Hurt, Eduard C., Grosjean, Henri, Branlant, Christiane
Pseudouridine (Ψ) residues were localized in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (UsnRNAs) by using the chemical mapping method. In contrast to vertebrate UsnRNAs, S....
Ségault, Véronique, Will, Cindy L., Polycarpou-Schwarz, Maria, Mattaj, Iain W., Branlant, Christiane, Lührmann, Reinhard
The function of conserved regions of the metazoan U5 snRNA was investigated by reconstituting U5 small nuclear ribonucleoprotein particles (snRNPs) from purified snRNP proteins and HeLa or Xenopus U5...
RNomics in Archaea reveals a further link between splicing of archaeal introns and rRNA processing
Tang, Thean Hock, Rozhdestvensky, Timofey S., D’Orval, Béatrice Clouet, Bortolin, Marie-Line, Huber, Harald, Charpentier, Bruno, ...
The bulge–helix–bulge (BHB) motif recognised by the archaeal splicing endonuclease is also found in the long processing stems of archaeal rRNA precursors in which it is cleaved to generate...
Branlant, Christiane, Krol, Alain, Machatt, Mohamed Ali, Pouyet, Jean, Ebel, Jean-Pierre, Edwards, Kaylene, ...
We determined 90% of the primary structure of E.coli MRE 600 23S rRNA by applying the sequencing gel technique to products of T1, S1, A and Naja oxiana nuclease digestion. Eight cistron...
Location of Ribosomal Protein Binding Sites on 16S Ribosomal RNA
Zimmermann, Robert A., Muto, Akira, Fellner, Peter, Ehresmann, Chantal, Branlant, Christiane
The distribution of ribosomal protein binding sites on the 16S ribosomal RNA molecule has been analyzed by limited ribonuclease hydrolysis of RNA-protein complexes, as well as by the interaction of...
Thouvenot, Benoit, Charpentier, Bruno, Branlant, Christiane
The Escherichia coli multi-promoter region of the gapA gene ensures a high level of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) production under various growth conditions. In the exponential...
Reconstitution of archaeal H/ACA small ribonucleoprotein complexes active in pseudouridylation
Charpentier, Bruno, Muller, Sébastien, Branlant, Christiane
Pseudouridine (Ψ) are frequently modified residues in RNA. In Eukarya, their formation is catalyzed by enzymes or by ribonucleoprotein complexes (RNPs) containing H/ACA snoRNAs. H/ACA sRNA and...
BEHM-ANSMANT, ISABELLE, URBAN, ALAN, MA, XIAOJU, YU, YI-TAO, MOTORIN, YURI, BRANLANT, CHRISTIANE
The Saccharomyces cerevisiae Pus7 protein was recently characterized as a novel RNA:pseudouridine (Ψ)-synthase acting at position 35 in U2 snRNA. However, U2 snRNA was the only potential substrate...
Manival, Xavier, Charron, Christophe, Fourmann, Jean-Baptiste, Godard, François, Charpentier, Bruno, Branlant, Christiane
In archaeal rRNAs, the isomerization of uridine into pseudouridine (Ψ) is achieved by the H/ACA sRNPs and the minimal set of proteins required for RNA:Ψ-synthase activity is the aCBF5–aNOP10...
MARMIER-GOURRIER, NATHALIE, CLÉRY, ANTOINE, SENTY-SÉGAULT, VÉRONIQUE, CHARPENTIER, BRUNO, SCHLOTTER, FLORENCE, LECLERC, FABRICE, ...
The 15.5-kD protein and its yeast homolog Snu13p bind U4 snRNA, U3 snoRNA, and the C/D box snoRNAs. In U4 snRNA, they associate with a helix-bulge-helix (K-turn) structure. U3 snoRNA contains two...
A previously unidentified activity of yeast and mouse RNA:pseudouridine synthases 1 (Pus1p) on tRNAs
Behm-Ansmant, Isabelle, Massenet, Séverine, Immel, FranÇoise, Patton, Jeffrey R., Motorin, Yuri, Branlant, Christiane
Mouse pseudouridine synthase 1 (mPus1p) was the first vertebrate RNA:pseudouridine synthase that was cloned and characterized biochemically. The mPus1p was previously found to catalyze Ψ formation...
Bakkour, Nadia, Lin, Yea-Lih, Maire, Sophie, Ayadi, Lilia, Mahuteau-Betzer, Florence, Nguyen, Chi Hung, ...
The development of multidrug-resistant viruses compromises antiretroviral therapy efficacy and limits therapeutic options. Therefore, it is an ongoing task to identify new targets for antiretroviral...
Muller, Sébastien, Fourmann, Jean-Baptiste, Loegler, Christine, Charpentier, Bruno, Branlant, Christiane
Protein aNOP10 has an essential scaffolding function in H/ACA sRNPs and its interaction with the pseudouridine(Ψ)-synthase aCBF5 is required for the RNA-guided RNA:Ψ-synthase activity. Recently,...
Behm-Ansmant, Isabelle, Branlant, Christiane, Motorin, Yuri
The RNA:pseudouridine (Ψ)-synthase family is one of the most complex families of RNA modification enzymes. Ten genes encoding putative RNA:Ψ-synthases have been identified in S. cerevisiae. Most of...
Muller, Sébastien, Leclerc, Fabrice, Behm-Ansmant, Isabelle, Fourmann, Jean-Baptiste, Charpentier, Bruno, Branlant, Christiane
How far do H/ACA sRNPs contribute to rRNA pseudouridylation in Archaea was still an open question. Hence here, by computational search in three Pyrococcus genomes, we identified seven H/ACA sRNAs and...
The Hsp90 chaperone controls the biogenesis of L7Ae RNPs through conserved machinery
Boulon, Séverine, Marmier-Gourrier, Nathalie, Pradet-Balade, Bérengère, Wurth, Laurence, Verheggen, Céline, Jády, Beáta E., ...
RNA-binding proteins of the L7Ae family are at the heart of many essential ribonucleoproteins (RNPs), including box C/D and H/ACA small nucleolar RNPs, U4 small nuclear RNP, telomerase, and messenger...