Chizu Kuroishi

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus (2007)

Kanaujia, Shankar Prasad, Ranjani, Chellamuthu Vasuki, Jeyakanthan, Jeyaraman, Baba, Seiki, Kuroishi, Chizu, Ebihara, Akio, ...

The aerobic Gram-positive bacterium Geobacillus kaustophilus is a bacillus species that was isolated from deep-sea sediment from the Mariana Trench. 1,4-Dihydroxy-2-naphthoate (DHNA) synthetase plays...

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus (2007)

Kanaujia, Shankar Prasad, Ranjani, Chellamuthu Vasuki, Jeyakanthan, Jeyaraman, Baba, Seiki, Kuroishi, Chizu, Ebihara, Akio, ...

The aerobic Gram-positive bacterium Geobacillus kaustophilus is a bacillus species that was isolated from deep-sea sediment from the Mariana Trench. 1,4-Dihydroxy-2-naphthoate (DHNA) synthetase plays...

Structural and mutational studies of the amino acid-editing domain from archaeal/eukaryal phenylalanyl-tRNA synthetase

Sasaki, Hiroshi M., Sekine, Shun-ichi, Sengoku, Toru, Fukunaga, Ryuya, Hattori, Motoyuki, Utsunomiya, Yukiko, ...

To achieve accurate aminoacylation of tRNAs with their cognate amino acids, errors in aminoacylation are corrected by the “editing” mechanism in several aminoacyl-tRNA synthetases....

Purification, crystallization and preliminary crystallographic analysis of the biotin–protein ligase from Pyrococcus horikoshii OT3

Bagautdinov, Bagautdin, Kuroishi, Chizu, Sugahara, Mitsuaki, Kunishima, Naoki

The biotin–protein ligase from P. horikoshii OT3 was overexpressed, purified, crystallized and cocrystallized with biotin, ADP and biotinyl-5′-AMP. The crystals belong to space group P21 and...

Structure of PIN-domain protein PH0500 from Pyrococcus horikoshii

Jeyakanthan, Jeyaraman, Inagaki, Eiji, Kuroishi, Chizu, Tahirov, Tahir H.

The structure of P. horikoshii OT3 protein PH0500 was determined by the multiple anomalous dispersion method and refined in two crystal forms. The protein is a dimer and has a PIN-domain fold.

Crystallization and avoiding the problem of hemihedral twinning in crystals of Δ1-pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus

Inagaki, Eiji, Takahashi, Hitomi, Kuroishi, Chizu, Tahirov, Tahir H.

Δ1-Pyrroline-5-carboxylate dehydrogenase from T. thermophilus has been crystallized in a citrate-bound form. The crystals exhibited hemihedral twinning and untwinned crystals were obtained by...

Crystallization and preliminary crystallographic analysis of the nickel-responsive regulator NikR from Pyrococcus horikoshii

Kitao, Tomoe, Kuroishi, Chizu, Tahirov, Tahir H.

Crystals of P. horikoshii NikR have been obtained in the presence and absence of Ni2+ and characterized by X-ray diffraction.

Purification, crystallization and preliminary X-ray crystallographic study of the l-fuculose-1-phosphate aldolase (FucA) from Thermus thermophilus HB8

Jeyakanthan, Jeyaraman, Taka, Junichiro, Kikuchi, Akihiro, Kuroishi, Chizu, Yutani, Katsuhide, Shiro, Yoshitugu

The crystallization and preliminary X-ray diffraction analysis of the l-fuculose-1-phosphate aldolase (FucA) from T. thermophilus HB8. Native diffraction data set was collected to a resolution of...

Expression, purification, crystallization and preliminary X-ray diffraction analysis of galactokinase from Pyrococcus horikoshii

Inagaki, Eiji, Sakamoto, Keiko, Obayashi, Naomi, Terada, Takaho, Shirouzu, Mikako, Bessho, Yoshitaka, ...

Galactokinase from P. horikoshii has been crystallized in both the apo form and as a ternary complex with α-d-galactose and an ATP analogue. The crystals were characterized by X-ray diffraction. The...

Crystal structure of the probable haloacid dehalogenase PH0459 from Pyrococcus horikoshii OT3

Arai, Ryoichi, Kukimoto-Niino, Mutsuko, Kuroishi, Chizu, Bessho, Yoshitaka, Shirouzu, Mikako, Yokoyama, Shigeyuki

PH0459, from the hyperthermophilic archaeon Pyrococcus horikoshii OT3, is a probable haloacid dehalogenase with a molecular mass of 26,725 Da. Here, we report the 2.0 Å crystal structure of PH0459...

Escherichia coli Cytosolic Glycerophosphodiester Phosphodiesterase (UgpQ) Requires Mg2+, Co2+, or Mn2+ for Its Enzyme Activity▿

Ohshima, Noriyasu, Yamashita, Saori, Takahashi, Naoko, Kuroishi, Chizu, Shiro, Yoshitsugu, Takio, Koji

Escherichia coli cytosolic glycerophosphodiester phosphodiesterase, UgpQ, functions in the absence of other proteins encoded by the ugp operon and requires Mg2+, Mn2+, or Co2+, in contrast to...