Yamada, Ayumi, Masutani, Chikahide, Iwai, Shigenori, Hanaoka, Fumio
Defects in the human gene XPV result in the variant form of the genetic disease xeroderma pigmentosum (XP-V). XPV encodes DNA polymerase η, a novel DNA polymerase that belongs to the UmuC/DinB/Rad30...
Molecular analysis of mutations in DNA polymerase η in xeroderma pigmentosum-variant patients
Broughton, Bernard C., Cordonnier, Agnes, Kleijer, Wim J., Jaspers, Nicolaas G. J., Fawcett, Heather, Raams, Anja, ...
Xeroderma pigmentosum variant (XP-V) cells are deficient in their ability to synthesize intact daughter DNA strands after UV irradiation. This deficiency results from mutations in the gene encoding...
Ura, Kiyoe, Araki, Marito, Saeki, Hideaki, Masutani, Chikahide, Ito, Takashi, Iwai, Shigenori, ...
To investigate the relationship between chromatin dynamics and nucleotide excision repair (NER), we have examined the effect of chromatin structure on the formation of two major classes of UV-induced...
Mechanisms of accurate translesion synthesis by human DNA polymerase η
Masutani, Chikahide, Kusumoto, Rika, Iwai, Shigenori, Hanaoka, Fumio
The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase η (pol η), which is involved in the replication of damaged DNA. Pol η catalyzes efficient and accurate translesion...
Jałoszyński, Paweł, Masutani, Chikahide, Hanaoka, Fumio, Perez, Ana Blanco, Nishimura, Susumu
Mutations in particular codons of c-Ha-ras have a strong activating potential, and an activated ras oncogene has been found in a number of human cancers. Using fragments of the human c-Ha-ras gene...
Error-prone bypass of certain DNA lesions by the human DNA polymerase κ
Ohashi, Eiji, Ogi, Tomoo, Kusumoto, Rika, Iwai, Shigenori, Masutani, Chikahide, Hanaoka, Fumio, ...
The Escherichia coli protein DinB is a newly identified error-prone DNA polymerase. Recently, a human homolog of DinB was identified and named DINB1. We report that the DINB1 gene encodes a DNA...
Palm Mutants in DNA Polymerases α and η Alter DNA Replication Fidelity and Translesion Activity
Niimi, Atsuko, Limsirichaikul, Siripan, Yoshida, Shonen, Iwai, Shigenori, Masutani, Chikahide, Hanaoka, Fumio, ...
We isolated active mutants in Saccharomyces cerevisiae DNA polymerase α that were associated with a defect in error discrimination. Among them, L868F DNA polymerase α has a spontaneous error...
Chemical synthesis and translesion replication of a cis–syn cyclobutane thymine–uracil dimer
Takasawa, Kohei, Masutani, Chikahide, Hanaoka, Fumio, Iwai, Shigenori
The cytosine base in DNA undergoes hydrolytic deamination at a considerable rate when UV radiation induces formation of a cyclobutane pyrimidine dimer (CPD) with an adjacent pyrimidine base. We have...
High-efficiency bypass of DNA damage by human DNA polymerase Q
Seki, Mineaki, Masutani, Chikahide, Yang, Lee Wei, Schuffert, Anthony, Iwai, Shigenori, Bahar, Ivet, ...
Endogenous DNA damage arises frequently, particularly apurinic (AP) sites. These must be dealt with by cells in order to avoid genotoxic effects. DNA polymerase θ is a newly identified enzyme...
Martomo, Stella A., Yang, William W., Wersto, Robert P., Ohkumo, Tsuyoshi, Kondo, Yuji, Yokoi, Masayuki, ...
Hypermutation in immunoglobulin genes produces a high frequency of substitutions of all four bases, which are likely generated by low-fidelity DNA polymerases. Indeed, humans deficient for DNA...
Nishi, Ryotaro, Okuda, Yuki, Watanabe, Eriko, Mori, Toshio, Iwai, Shigenori, Masutani, Chikahide, ...
Xeroderma pigmentosum group C (XPC) protein plays a key role in DNA damage recognition in global genome nucleotide excision repair (NER). The protein forms in vivo a heterotrimeric complex involving...
Erroneous incorporation of oxidized DNA precursors by Y-family DNA polymerases
Shimizu, Masatomi, Gruz, Petr, Kamiya, Hiroyuki, Kim, Su-Ryang, Pisani, Francesca M., Masutani, Chikahide, ...
Deranged oxidative metabolism is a property of many tumour cells. Oxidation of the deoxynucleotide triphosphate (dNTP) pool, as well as DNA, is a major cause of genome instability. Here, we report...
A multistep damage recognition mechanism for global genomic nucleotide excision repair
Sugasawa, Kaoru, Okamoto, Tomoko, Shimizu, Yuichiro, Masutani, Chikahide, Iwai, Shigenori, Hanaoka, Fumio
A mammalian nucleotide excision repair (NER) factor, the XPC–HR23B complex, can specifically bind to certain DNA lesions and initiate the cell-free repair reaction. Here we describe a detailed...
Yamada, Ayumi, Masutani, Chikahide, Iwai, Shigenori, Hanaoka, Fumio
Defects in the human gene XPV result in the variant form of the genetic disease xeroderma pigmentosum (XP-V). XPV encodes DNA polymerase η, a novel DNA polymerase that belongs to the UmuC/DinB/Rad30...
Molecular analysis of mutations in DNA polymerase η in xeroderma pigmentosum-variant patients
Broughton, Bernard C., Cordonnier, Agnes, Kleijer, Wim J., Jaspers, Nicolaas G. J., Fawcett, Heather, Raams, Anja, ...
Xeroderma pigmentosum variant (XP-V) cells are deficient in their ability to synthesize intact daughter DNA strands after UV irradiation. This deficiency results from mutations in the gene encoding...
Ura, Kiyoe, Araki, Marito, Saeki, Hideaki, Masutani, Chikahide, Ito, Takashi, Iwai, Shigenori, ...
To investigate the relationship between chromatin dynamics and nucleotide excision repair (NER), we have examined the effect of chromatin structure on the formation of two major classes of UV-induced...
Mechanisms of accurate translesion synthesis by human DNA polymerase η
Masutani, Chikahide, Kusumoto, Rika, Iwai, Shigenori, Hanaoka, Fumio
The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase η (pol η), which is involved in the replication of damaged DNA. Pol η catalyzes efficient and accurate translesion...
Jałoszyński, Paweł, Masutani, Chikahide, Hanaoka, Fumio, Perez, Ana Blanco, Nishimura, Susumu
Mutations in particular codons of c-Ha-ras have a strong activating potential, and an activated ras oncogene has been found in a number of human cancers. Using fragments of the human c-Ha-ras gene...
A multistep damage recognition mechanism for global genomic nucleotide excision repair
Sugasawa, Kaoru, Okamoto, Tomoko, Shimizu, Yuichiro, Masutani, Chikahide, Iwai, Shigenori, Hanaoka, Fumio
A mammalian nucleotide excision repair (NER) factor, the XPC–HR23B complex, can specifically bind to certain DNA lesions and initiate the cell-free repair reaction. Here we describe a detailed...
Error-prone bypass of certain DNA lesions by the human DNA polymerase κ
Ohashi, Eiji, Ogi, Tomoo, Kusumoto, Rika, Iwai, Shigenori, Masutani, Chikahide, Hanaoka, Fumio, ...
The Escherichia coli protein DinB is a newly identified error-prone DNA polymerase. Recently, a human homolog of DinB was identified and named DINB1. We report that the DINB1 gene encodes a DNA...
Palm Mutants in DNA Polymerases α and η Alter DNA Replication Fidelity and Translesion Activity
Niimi, Atsuko, Limsirichaikul, Siripan, Yoshida, Shonen, Iwai, Shigenori, Masutani, Chikahide, Hanaoka, Fumio, ...
We isolated active mutants in Saccharomyces cerevisiae DNA polymerase α that were associated with a defect in error discrimination. Among them, L868F DNA polymerase α has a spontaneous error...
Chemical synthesis and translesion replication of a cis–syn cyclobutane thymine–uracil dimer
Takasawa, Kohei, Masutani, Chikahide, Hanaoka, Fumio, Iwai, Shigenori
The cytosine base in DNA undergoes hydrolytic deamination at a considerable rate when UV radiation induces formation of a cyclobutane pyrimidine dimer (CPD) with an adjacent pyrimidine base. We have...
High-efficiency bypass of DNA damage by human DNA polymerase Q
Seki, Mineaki, Masutani, Chikahide, Yang, Lee Wei, Schuffert, Anthony, Iwai, Shigenori, Bahar, Ivet, ...
Endogenous DNA damage arises frequently, particularly apurinic (AP) sites. These must be dealt with by cells in order to avoid genotoxic effects. DNA polymerase θ is a newly identified enzyme...
Martomo, Stella A., Yang, William W., Wersto, Robert P., Ohkumo, Tsuyoshi, Kondo, Yuji, Yokoi, Masayuki, ...
Hypermutation in immunoglobulin genes produces a high frequency of substitutions of all four bases, which are likely generated by low-fidelity DNA polymerases. Indeed, humans deficient for DNA...
Nishi, Ryotaro, Okuda, Yuki, Watanabe, Eriko, Mori, Toshio, Iwai, Shigenori, Masutani, Chikahide, ...
Xeroderma pigmentosum group C (XPC) protein plays a key role in DNA damage recognition in global genome nucleotide excision repair (NER). The protein forms in vivo a heterotrimeric complex involving...
Erroneous incorporation of oxidized DNA precursors by Y-family DNA polymerases
Shimizu, Masatomi, Gruz, Petr, Kamiya, Hiroyuki, Kim, Su-Ryang, Pisani, Francesca M., Masutani, Chikahide, ...
Deranged oxidative metabolism is a property of many tumour cells. Oxidation of the deoxynucleotide triphosphate (dNTP) pool, as well as DNA, is a major cause of genome instability. Here, we report...
Ohkumo, Tsuyoshi, Kondo, Yuji, Yokoi, Masayuki, Tsukamoto, Tetsuya, Yamada, Ayumi, Sugimoto, Taiki, ...
DNA polymerase η (Pol η) is the product of the Polh gene, which is responsible for the group variant of xeroderma pigmentosum, a rare inherited recessive disease which is characterized by...
McDonald, John P., Frank, Ekaterina G., Plosky, Brian S., Rogozin, Igor B., Masutani, Chikahide, Hanaoka, Fumio, ...
Recent studies suggest that DNA polymerase η (polη) and DNA polymerase ι (polι) are involved in somatic hypermutation of immunoglobulin variable genes. To test the role of polι in generating...