Graddis, Thomas J., Klig, Lisa S., Yanofsky, Charles, Oxender, Dale L.
Availability of the three-dimensional structure of the trp repressor of Escherichia coli and a large group of repressor mutants has permitted the identification and analysis of mutants with...
Sarsero, Joseph P., Merino, Enrique, Yanofsky, Charles
Strains of Bacillus subtilis containing a temperature-sensitive tryptophanyl-tRNA synthetase produce elevated levels of the tryptophan pathway enzymes, when grown at high temperatures in the presence...
Khodursky, Arkady B., Peter, Brian J., Cozzarelli, Nicholas R., Botstein, David, Brown, Patrick O., Yanofsky, Charles
We investigated the global changes in mRNA abundance in Escherichia coli elicited by various perturbations of tryptophan metabolism. To do so we printed DNA microarrays containing 95% of all...
Spraggon, Glen, Kim, Choel, Nguyen-Huu, Xuong, Yee, Muh-Ching, Yanofsky, Charles, Mills, Stanley E.
The crystal structure of anthranilate synthase (AS) from Serratia marcescens, a mesophilic bacterium, has been solved in the presence of its substrates, chorismate and glutamine, and one product,...
Gong, Feng, Ito, Koichi, Nakamura, Yoshikazu, Yanofsky, Charles
Expression of the tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and tryptophan-induced transcription antitermination. In a previous study, we reproduced the...
Role of Ribosome Release in Regulation of tna Operon Expression in Escherichia coli
Konan, Kouacou Vincent, Yanofsky, Charles
Expression of the degradative tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and tryptophan-induced transcription antitermination. In cultures growing in the...
Konan, Kouacou Vincent, Yanofsky, Charles
Expression of the tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and by tryptophan-induced transcription antitermination. Tryptophan induction prevents...
Sarsero, Joseph P., Merino, Enrique, Yanofsky, Charles
Computer analysis of the Bacillus subtilis genome sequence revealed a gene with no previously attributed function, yhaG, specifying a transcript containing a presumptive binding site for the...
Berka, Randy M., Cui, Xianju, Yanofsky, Charles
DNA microarrays comprising ≈95% of the Bacillus subtilis annotated protein coding ORFs were deployed to generate a series of snapshots of genomewide transcriptional changes that occur when cells...
Regulation of transcription of the tryptophanase operon requires that translation of its leader peptide coding region, tnaC, be coupled with its transcription. We show in vitro that a transcription...
Duplication-Translocations of Tryptophan Operon Genes in Escherichia coli
Jackson, Ethel Noland, Yanofsky, Charles
Mutants of Escherichia coli were selected in which a single mutational event had both relieved the polar effect of an early trpE mutation on trpB and simultaneously released the expression of trpB...
Denney, Richard M., Yanofsky, Charles
We have isolated a series of nondefective φ80 specialized transducing phage which carry segments of the Salmonella typhimurium trp operon. These phage were obtained from a lysogenic derivative of a...
Pseudomonas putida Tryptophan Synthetase: Partial Sequence of the α Subunit
Crawford, Irving P., Yanofsky, Charles
There is 50% identity in the sequences of the first 50 residues of the α chains of Escherichia coli and Pseudomonas putida. No deletions or additions of residues are found in this region, except for...
Rose, John K., Yanofsky, Charles
Measurements of the time required for transcription of regions of known genetic length in the tryptophan operon of Escherichia coli indicate that there are no major unidentified structural genes in...
Mosteller, Raymond D., Yanofsky, Charles
When a growing culture of a tryptophan auxotroph of Escherichia coli is transferred to a tryptophan-free medium, the bacteria exhaust their supply of Trp-transfer ribonucleic acid (tRNA). Under these...
Ito, Junetsu, Cox, Edward C., Yanofsky, Charles
A procedure employed in the purification of anthranilate synthetase component I of Escherichia coli is described. The purified component appears homogeneous by starch gel electrophoresis and by...
Ito, Junetsu, Yanofsky, Charles
The properties of the anthranilate synthetase complex and its separated subunits were compared in catalyzing the anthranilate synthetase reaction, chorismate + l-glutamine or NH4+ → anthranilate,...
Amino Terminal Sequence of the Tryptophan Synthetase Alpha Chain of Serratia marcescens
Li, Shoei-Lung, Drapeau, Gabriel R., Yanofsky, Charles
The sequence of the amino terminal 28 residues of the tryptophan synthetase α chain of Serratia marcescens is presented and compared with the related sęquences of α chains of other bacteria.
Jackson, Ethel Noland, Yanofsky, Charles
The trpD gene specifies a polypeptide which has both glutamine amidotransferase and phosphoribosyl anthranilate (PRA) transferase activities. Deletions fusing segments of trpD to the gene preceding...
Downie, J. Allan, Langman, Lyndall, Cox, Graeme B., Yanofsky, Charles, Gibson, Frank
The unc operon of Escherichia coli was split into two fragments by the restriction endonuclease HindIII. The operator-proximal portion was cloned into plasmid pACYC184, forming plasmid pAN51, which...
TRYPTOPHAN SYNTHETASE LEVELS IN ESCHERICHIA COLI, SHIGELLA DYSENTERIAE, AND TRANSDUCTION HYBRIDS
Eisenstein, Richard B., Yanofsky, Charles
Eisenstein, Richard B. (Western Reserve University, Cleveland, Ohio) and Charles Yanofsky. Tryptophan synthetase levels in Escherichia coli, Shigella dysenteriae, and transduction hybrids. J....
Mutants of Escherichia coli with an Altered Tryptophanyl-Transfer Ribonucleic Acid Synthetase
Doolittle, W. Ford, Yanofsky, Charles
Fourteen mutant strains of Escherichia coli were examined, each of which requires tryptophan for growth but is unaltered in any of the genes of the tryptophan biosynthetic operon. The genetic lesions...
Berger, Hillard, Brammar, William J., Yanofsky, Charles
Frameshift mutant trpA21 was isolated after ultraviolet treatment and frameshift mutant trpA540 after ICR191-A (an acridine derivative) treatment of wild-type Escherichia coli K-12. The A proteins of...
Mutator Gene Studies in Escherichia coli
Cox, Edward C., Yanofsky, Charles
An Escherichia coli mutator gene, mutT, has been shown by P1-mediated crosses to map between the leucine and azide loci. The mutT1 and azi-r alleles cotransduce with a frequency of >92%. In...
Mechanism Studies of Suppressor-Gene Action1
Brody, Stuart, Yanofsky, Charles
Brody, Stuart (Stanford University, Stanford, Calif.), and Charles Yanofsky. Mechanism studies of suppressor-gene action. J. Bacteriol. 90:687–695. 1965.—Mutations which change the primary...
Efficient cloning of genes of Neurospora crassa
Vollmer, Steven J., Yanofsky, Charles
We have constructed a genomic library of Neurospora crassa DNA in a cosmid vector that contains the dominant selectable marker for benomyl resistance. The library is arranged to permit the rapid...
THE ENZYMATIC ACTIVITY ASSOCIATED WITH THE PROTEIN IMMUNOLOGICALLY RELATED TO TRYPTOPHAN SYNTHETASE*
Nucleotide sequence of the leader region of the phenylalanine operon of Escherichia coli
Zurawski, Gerard, Brown, Keith, Killingly, David, Yanofsky, Charles
The pheA structural gene of the phenylalanine operon of Escherichia coli is preceded by a transcribed leader region of about 170 nucleotide pairs. In vitro transcription of plasmids and restriction...
Interaction of the Operator of the Tryptophan Operon with Repressor
Rose, John K., Yanofsky, Charles
Transcription studies in vitro on repression of the tryptophan operon of Escherichia coli show that partially purified trp repressor binds specifically to DNA containing the trp operator with a...
Bronson, Morley J., Squires, Craig, Yanofsky, Charles
Mutants with internal deletions that terminate near the operator end of the tryptophan operon of E. coli were used in studies on the nucleotide sequence at the 5′ end of the messenger RNA...
Plasmid ColE1 as a Molecular Vehicle for Cloning and Amplification of DNA
Hershfield, Vickers, Boyer, Herbert W., Yanofsky, Charles, Lovett, Michael A., Helinski, Donald R.
DNA fragments obtained from EcoRI endonuclease digestion of bacteriophage ϕ80pt190 (trp+) and the plasmid ColE1 were covalently joined with polynucleotide ligase. Transformation of Escherichia coli...
Quinolinic Acid Accumulation in the Conversion of 3-Hydroxyanthranilic Acid to Niacin in Neurospora*
Yang, Wen-Jen, Yanofsky, Charles
The anti-TRAP protein (AT), encoded by the rtpA gene of Bacillus subtilis, can bind to and inhibit the tryptophan-activated trp RNA-binding attenuation protein (TRAP). AT binding can prevent TRAP...
Overexpression of tnaC of Escherichia coli Inhibits Growth by Depleting tRNA2Pro Availability
Gong, Ming, Gong, Feng, Yanofsky, Charles
Transcription of the tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and tryptophan-induced transcription antitermination. Induction results from ribosome...
Translational Coupling during Expression of the Tryptophan Operon of ESCHERICHIA COLI
Oppenheim, Daniel S., Yanofsky, Charles
E. coli trpE polar mutations are 10 times more polar on trpD gene expression than on downstream (trpC, B, or A) gene expression. This effect was shown to be the result of "translational coupling," in...
Cruz-Vera, Luis Rogelio, Gong, Ming, Yanofsky, Charles
Studies in vitro have established that free tryptophan induces tna operon expression by binding to the ribosome that has just completed synthesis of TnaC-tRNAPro, the peptidyl-tRNA precursor of the...
Sarsero, Joseph P., Merino, Enrique, Yanofsky, Charles
Strains of Bacillus subtilis containing a temperature-sensitive tryptophanyl-tRNA synthetase produce elevated levels of the tryptophan pathway enzymes, when grown at high temperatures in the presence...
Khodursky, Arkady B., Peter, Brian J., Cozzarelli, Nicholas R., Botstein, David, Brown, Patrick O., Yanofsky, Charles
We investigated the global changes in mRNA abundance in Escherichia coli elicited by various perturbations of tryptophan metabolism. To do so we printed DNA microarrays containing 95% of all...
Spraggon, Glen, Kim, Choel, Nguyen-Huu, Xuong, Yee, Muh-Ching, Yanofsky, Charles, Mills, Stanley E.
The crystal structure of anthranilate synthase (AS) from Serratia marcescens, a mesophilic bacterium, has been solved in the presence of its substrates, chorismate and glutamine, and one product,...
Gong, Feng, Ito, Koichi, Nakamura, Yoshikazu, Yanofsky, Charles
Expression of the tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and tryptophan-induced transcription antitermination. In a previous study, we reproduced the...
Role of Ribosome Release in Regulation of tna Operon Expression in Escherichia coli
Konan, Kouacou Vincent, Yanofsky, Charles
Expression of the degradative tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and tryptophan-induced transcription antitermination. In cultures growing in the...
Konan, Kouacou Vincent, Yanofsky, Charles
Expression of the tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and by tryptophan-induced transcription antitermination. Tryptophan induction prevents...
Sarsero, Joseph P., Merino, Enrique, Yanofsky, Charles
Computer analysis of the Bacillus subtilis genome sequence revealed a gene with no previously attributed function, yhaG, specifying a transcript containing a presumptive binding site for the...
Berka, Randy M., Cui, Xianju, Yanofsky, Charles
DNA microarrays comprising ≈95% of the Bacillus subtilis annotated protein coding ORFs were deployed to generate a series of snapshots of genomewide transcriptional changes that occur when cells...
Regulation of transcription of the tryptophanase operon requires that translation of its leader peptide coding region, tnaC, be coupled with its transcription. We show in vitro that a transcription...
Duplication-Translocations of Tryptophan Operon Genes in Escherichia coli
Jackson, Ethel Noland, Yanofsky, Charles
Mutants of Escherichia coli were selected in which a single mutational event had both relieved the polar effect of an early trpE mutation on trpB and simultaneously released the expression of trpB...
Denney, Richard M., Yanofsky, Charles
We have isolated a series of nondefective φ80 specialized transducing phage which carry segments of the Salmonella typhimurium trp operon. These phage were obtained from a lysogenic derivative of a...
Pseudomonas putida Tryptophan Synthetase: Partial Sequence of the α Subunit
Crawford, Irving P., Yanofsky, Charles
There is 50% identity in the sequences of the first 50 residues of the α chains of Escherichia coli and Pseudomonas putida. No deletions or additions of residues are found in this region, except for...
Rose, John K., Yanofsky, Charles
Measurements of the time required for transcription of regions of known genetic length in the tryptophan operon of Escherichia coli indicate that there are no major unidentified structural genes in...
Mosteller, Raymond D., Yanofsky, Charles
When a growing culture of a tryptophan auxotroph of Escherichia coli is transferred to a tryptophan-free medium, the bacteria exhaust their supply of Trp-transfer ribonucleic acid (tRNA). Under these...
Ito, Junetsu, Cox, Edward C., Yanofsky, Charles
A procedure employed in the purification of anthranilate synthetase component I of Escherichia coli is described. The purified component appears homogeneous by starch gel electrophoresis and by...
Ito, Junetsu, Yanofsky, Charles
The properties of the anthranilate synthetase complex and its separated subunits were compared in catalyzing the anthranilate synthetase reaction, chorismate + l-glutamine or NH4+ → anthranilate,...
Amino Terminal Sequence of the Tryptophan Synthetase Alpha Chain of Serratia marcescens
Li, Shoei-Lung, Drapeau, Gabriel R., Yanofsky, Charles
The sequence of the amino terminal 28 residues of the tryptophan synthetase α chain of Serratia marcescens is presented and compared with the related sęquences of α chains of other bacteria.
Jackson, Ethel Noland, Yanofsky, Charles
The trpD gene specifies a polypeptide which has both glutamine amidotransferase and phosphoribosyl anthranilate (PRA) transferase activities. Deletions fusing segments of trpD to the gene preceding...
Downie, J. Allan, Langman, Lyndall, Cox, Graeme B., Yanofsky, Charles, Gibson, Frank
The unc operon of Escherichia coli was split into two fragments by the restriction endonuclease HindIII. The operator-proximal portion was cloned into plasmid pACYC184, forming plasmid pAN51, which...