Lonhienne, Thierry, Baise, Etienne, Feller, Georges, Bouriotis, Vassilis, Gerday, Charles
Isothermal titration calorimetry has been applied to the determination of the kinetic parameters of chitinases (EC 3.2.1.14) by monitoring the heat released during the hydrolysis of chitin glycosidic...
Lonhienne, Thierry, Zoidakis, Jérôme, Vorgias, Constantinos E., Feller, Georges, Gerday, Charles, Bouriotis, Vassilis
The gene archb encoding for the cell-bound chitobiase from the Antarctic Gram-positive bacterium Arthrobacter sp. TAD20 was cloned and expressed in Escherichia coli in a soluble form. The mature...
Lonhienne, Thierry, Mavromatis, Konstantinos, Vorgias, Constantin E., Buchon, Laurent, Gerday, Charles, Bouriotis, Vassilis
Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA...
Lonhienne, Thierry, Mavromatis, Konstantinos, Vorgias, Constantin E., Buchon, Laurent, Gerday, Charles, Bouriotis, Vassilis
Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA...
Feller, Georges, Le Bussy, Olivier, Gerday, Charles
α-Amylase from the antarctic psychrophile Alteromonas haloplanktis is synthesized at 0 ± 2°C by the wild strain. This heat-labile α-amylase folds correctly when overexpressed in Escherichia coli,...
Xu, Ying, Feller, Georges, Gerday, Charles, Glansdorff, Nicolas
The enzyme ornithine carbamoyltransferase (OTCase) of Moritella abyssi (OTCaseMab), a new, strictly psychrophilic and piezophilic bacterial species, was purified. OTCaseMab displays maximal activity...
Xu, Ying, Feller, Georges, Gerday, Charles, Glansdorff, Nicolas
Adapting metabolic enzymes of microorganisms to low temperature environments may require a difficult compromise between velocity and affinity. We have investigated catalytic efficiency in a key...
Garsoux, Geneviève, Lamotte, Josette, Gerday, Charles, Feller, Georges
The cold-adapted cellulase CelG has been purified from the culture supernatant of the Antarctic bacterium Pseudoalteromonas haloplanktis and the gene coding for this enzyme has been cloned, sequenced...
Siddiqui, Khawar S., Feller, Georges, D'Amico, Salvino, Gerday, Charles, Giaquinto, Laura, Cavicchioli, Ricardo
The cold-active α-amylase from the Antarctic bacterium Pseudoalteromonas haloplanktis (AHA) is the largest known multidomain enzyme that displays reversible thermal unfolding (around 30°C)...
Role of Disulfide Bridges in the Activity and Stability of a Cold-Active α-Amylase
Siddiqui, Khawar Sohail, Poljak, Anne, Guilhaus, Michael, Feller, Georges, D'Amico, Salvino, Gerday, Charles, ...
The cold-adapted α-amylase from Pseudoalteromonas haloplanktis unfolds reversibly and cooperatively according to a two-state mechanism at 30°C and unfolds reversibly and sequentially with two...
Georlette, Daphné, Blaise, Vinciane, Bouillenne, Fabrice, Damien, Benjamin, Thorbjarnardóttir, Sigridur H., Depiereux, Eric, ...
In the last few years, an increased attention has been focused on NAD+-dependent DNA ligases. This is mostly due to their potential use as antibiotic targets, because effective inhibition of these...
Lonhienne, Thierry, Mavromatis, Konstantinos, Vorgias, Constantin E., Buchon, Laurent, Gerday, Charles, Bouriotis, Vassilis
Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA...
Feller, Georges, Le Bussy, Olivier, Gerday, Charles
α-Amylase from the antarctic psychrophile Alteromonas haloplanktis is synthesized at 0 ± 2°C by the wild strain. This heat-labile α-amylase folds correctly when overexpressed in Escherichia coli,...
Xu, Ying, Feller, Georges, Gerday, Charles, Glansdorff, Nicolas
The enzyme ornithine carbamoyltransferase (OTCase) of Moritella abyssi (OTCaseMab), a new, strictly psychrophilic and piezophilic bacterial species, was purified. OTCaseMab displays maximal activity...
Xu, Ying, Feller, Georges, Gerday, Charles, Glansdorff, Nicolas
Adapting metabolic enzymes of microorganisms to low temperature environments may require a difficult compromise between velocity and affinity. We have investigated catalytic efficiency in a key...
Garsoux, Geneviève, Lamotte, Josette, Gerday, Charles, Feller, Georges
The cold-adapted cellulase CelG has been purified from the culture supernatant of the Antarctic bacterium Pseudoalteromonas haloplanktis and the gene coding for this enzyme has been cloned, sequenced...
Siddiqui, Khawar S., Feller, Georges, D'Amico, Salvino, Gerday, Charles, Giaquinto, Laura, Cavicchioli, Ricardo
The cold-active α-amylase from the Antarctic bacterium Pseudoalteromonas haloplanktis (AHA) is the largest known multidomain enzyme that displays reversible thermal unfolding (around 30°C)...
Role of Disulfide Bridges in the Activity and Stability of a Cold-Active α-Amylase
Siddiqui, Khawar Sohail, Poljak, Anne, Guilhaus, Michael, Feller, Georges, D'Amico, Salvino, Gerday, Charles, ...
The cold-adapted α-amylase from Pseudoalteromonas haloplanktis unfolds reversibly and cooperatively according to a two-state mechanism at 30°C and unfolds reversibly and sequentially with two...
Georlette, Daphné, Blaise, Vinciane, Bouillenne, Fabrice, Damien, Benjamin, Thorbjarnardóttir, Sigridur H., Depiereux, Eric, ...
In the last few years, an increased attention has been focused on NAD+-dependent DNA ligases. This is mostly due to their potential use as antibiotic targets, because effective inhibition of these...
Molecular basis of cold adaptation.
D'Amico, Salvino, Claverie, Paule, Collins, Tony, Georlette, Daphné, Gratia, Emmanuelle, Hoyoux, Anne, ...
Cold-adapted, or psychrophilic, organisms are able to thrive at low temperatures in permanently cold environments, which in fact characterize the greatest proportion of our planet. Psychrophiles...
Psychrophilic microorganisms: challenges for life
D'Amico, Salvino, Collins, Tony, Marx, Jean-Claude, Feller, Georges, Gerday, Charles
The ability of psychrophiles to survive and proliferate at low temperatures implies that they have overcome key barriers inherent to permanently cold environments. These challenges include: reduced...
Sonan, Guillaume K., Receveur-Brechot, Véronique, Duez, Colette, Aghajari, Nushin, Czjzek, Mirjam, Haser, Richard, ...
The psychrophilic cellulase, Cel5G, from the Antarctic bacterium Pseudoalteromonas haloplanktis is composed of a catalytic module (CM) joined to a carbohydrate-binding module (CBM) by an unusually...
Structural basis of α-amylase activation by chloride
Aghajari, Nushin, Feller, Georges, Gerday, Charles, Haser, Richard
To further investigate the mechanism and function of allosteric activation by chloride in some α-amylases, the structure of the bacterial α-amylase from the psychrophilic micro-organism...