IL-18 Does not Increase Allergic Airway Disease in Mice When Produced by BCG. (2008)
Amniai, Latiffa, Biet, Franck, Marquillies, Philippe, Locht, Camille, Pestel, Joël, Tonnel, André-Bernard, ...
Whilst BCG inhibits allergic airway responses in murine models, IL-18 has adversary effects depending on its environment. We therefore constructed a BCG strain producing murine IL-18 (BCG-IL-18) and...
IL-18 Does not Increase Allergic Airway Disease in Mice When Produced by BCG. (2008)
Amniai, Latiffa, Biet, Franck, Marquillies, Philippe, Locht, Camille, Pestel, Joël, Tonnel, André-Bernard, ...
Whilst BCG inhibits allergic airway responses in murine models, IL-18 has adversary effects depending on its environment. We therefore constructed a BCG strain producing murine IL-18 (BCG-IL-18) and...
Live Attenuated B. pertussis as a Single-Dose Nasal Vaccine against Whooping Cough (2006)
Nathalie Mielcarek, Anne-Sophie Debrie, Dominique Raze, Julie Bertout, Carine Rouanet, Amena Ben Younes, ...
Pertussis is still among the principal causes of death worldwide, and its incidence is increasing even in countries with high vaccine coverage. Although all age groups are susceptible, it is most...
Vaccinations : actualités et perspectives (1999)
Bégué, Pierre, Boumsell, Laurence, Buisson, Yves, Carrat, Fabrice, Fuchs, Florence, Gilberg, Serge, ...
297 pages, figures, tableaux, références bibliographiques disséminées
Glutathione S-Transferases of 28kDa as Major Vaccine Candidates against Schistosomiasis (1998)
Gilles Riveau, Odile Poulain-Godefroy, Loïc Dupré, Franck Remoué, Nathalie Mielcarek, Camille Locht, ...
For the development of vaccine strategies to generate efficient protection against chronic infections such as parasitic diseases, and more precisely schistosomiasis, controlling pathology could be...
Glutathione S-transferases of 28kDa as major vaccine candidates against schistosomiasis (1998)
Riveau,Gilles, Poulain-Godefroy,Odile, Dupré,Loïc, Remoué,Franck, Mielcarek,Nathalie, Locht,Camille, ...
For the development of vaccine strategies to generate efficient protection against chronic infections such as parasitic diseases, and more precisely schistosomiasis, controlling pathology could be...
Menozzi, Franco D., Bischoff, Rainer, Fort, Emmanuelle, Brennan, Michael J., Locht, Camille
Although it generally is accepted that the interaction of Mycobacterium tuberculosis with alveolar macrophages is a key step in the pathogenesis of tuberculosis, interactions with other cell types,...
Mazars, Edith, Lesjean, Sarah, Banuls, Anne-Laure, Gilbert, Michèle, Vincent, Véronique, Gicquel, Brigitte, ...
The worldwide threat of tuberculosis to human health emphasizes the need to develop novel approaches to a global epidemiological surveillance. The current standard for Mycobacterium tuberculosis...
Supply, Philip, Lesjean, Sarah, Savine, Evgueni, Kremer, Kristin, Van Soolingen, Dick, Locht, Camille
Large-scale genotyping of Mycobacterium tuberculosis is especially challenging, as the current typing methods are labor-intensive and the results are difficult to compare among laboratories. Here,...
Antoine, Rudy, Alonso, Sylvie, Raze, Dominique, Coutte, Loïc, Lesjean, Sarah, Willery, Eve, ...
An in silico scan of the partially completed genome sequence of Bordetella pertussis and analyses of transcriptional fusions generated with a new integrational vector were used to identify new...
Mielcarek, Nathalie, Nordström, Inger, Menozzi, Franco D., Locht, Camille, Holmgren, Jan
Intranasal administration of live attenuated Bordetella pertussis, from which the pertussis toxin gene has been deleted, has previously been shown to give rise to high levels of serum immunoglobulin...
Bordetella pertussis TonB, a Bvg-Independent Virulence Determinant
Pradel, Elizabeth, Guiso, Nicole, Menozzi, Franco D., Locht, Camille
In gram-negative bacteria, high-affinity iron uptake requires the TonB/ExbB/ExbD envelope complex to release iron chelates from their specific outer membrane receptors into the periplasm. Based on...
Coppens, Isabelle, Alonso, Sylvie, Antoine, Rudy, Jacob-Dubuisson, Françoise, Renauld-Mongénie, Geneviève, Jacobs, Eric, ...
Neisseria meningitidis serogroup B infections are among the major causes of fulminant septicemia and meningitis, especially severe in young children, and no broad vaccine is available yet. Because of...
Alonso, Sylvie, Pethe, Kévin, Mielcarek, Nathalie, Raze, Dominique, Locht, Camille
Pertussis toxin (PT) and filamentous hemagglutinin (FHA) are two major virulence factors of Bordetella pertussis. FHA is the main adhesin, whereas PT is a toxin with an A-B structure, in which the A...
Pradel, Elizabeth, Locht, Camille
A new gene from Bordetella bronchiseptica, bfrZ encoding a putative siderophore receptor, was identified in a Fur-repressor titration assay. A bfrZ null mutant was constructed by allelic exchange....
Kremer, Laurent, Estaquier, Jérôme, Wolowczuk, Isabelle, Biet, Franck, Ameisen, Jean-Claude, Locht, Camille
It has previously been reported that inhibition of delayed-type hypersensitivity-mediating functions of T cells during mycobacterial infection in mice is haplotype dependent. In the present study, we...
Identification of a New DNA Region Specific for Members of Mycobacterium tuberculosis Complex
Magdalena, Juana, Vachée, Anne, Supply, Philip, Locht, Camille
The successful use of DNA amplification for the detection of tuberculous mycobacteria crucially depends on the choice of the target sequence, which ideally should be present in all tuberculous...
Magdalena, Juana, Supply, Philip, Locht, Camille
A PCR procedure based on the intergenic region (IR) separating two genes encoding a recently identified mycobacterial two-component system, named SenX3-RegX3, was developed and was shown to be...
Pradel, Elizabeth, Guiso, Nicole, Locht, Camille
A Fur titration assay was used to isolate DNA fragments bearing putative Fur binding sites (FBS) from a partial Bordetella bronchiseptica genomic DNA library. A recombinant plasmid bearing a 3.5-kb...
Poulain-Godefroy, Odile, Mielcarek, Nathalie, Ivanoff, Nathalie, Remoué, Franck, Schacht, Anne-Marie, Phillips, Nigel, ...
In an attempt to increase the immunogenicity of mucosally delivered antigens, we incorporated the Bordetella pertussis filamentous hemagglutinin (FHA) adhesin into liposomes containing the...
Kremer, Laurent, Dupré, Loïc, Riveau, Gilles, Capron, André, Locht, Camille
A major goal of current vaccine development is the induction of strong immune responses against protective antigens delivered by mucosal routes. One of the most promising approaches in that respect...
Pethe, Kevin, Bifani, Pablo, Drobecq, Hervé, Sergheraert, Christian, Debrie, Anne-Sophie, Locht, Camille, ...
Mycobacterium tuberculosis and Mycobacterium bovis bacillus Calmette–Guérin produce a heparin-binding hemagglutinin adhesin (HBHA) required for extrapulmonary dissemination and a laminin-binding...
Subtilisin-like autotransporter serves as maturation protease in a bacterial secretion pathway
Coutte, Loic, Antoine, Rudy, Drobecq, Hervé, Locht, Camille, Jacob-Dubuisson, Françoise
Proteins of Gram-negative bacteria destined to the extracellular milieu must cross the two cellular membranes and then fold at the appropriate time and place. The synthesis of a precursor may be a...
Ewann, Fanny, Jackson, Mary, Pethe, Kevin, Cooper, Andrea, Mielcarek, Nathalie, Ensergueix, Danielle, ...
Adaptive regulation of gene expression in response to environmental changes is a general property of bacterial pathogens. By screening an ordered transposon mutagenesis library of Mycobacterium...
Roduit, Caroline, Bozzotti, Paola, Mielcarek, Nathalie, Lambert, Paul-Henri, Del Giudice, Giuseppe, Locht, Camille, ...
A significant resurgence of early cases of pertussis is being observed in infants too young to have yet completed their three-dose vaccination schedule. In this study, murine models of immunization...
Alonso, Sylvie, Reveneau, Nathalie, Pethe, Kévin, Locht, Camille
Bordetella pertussis, the etiological agent of whooping cough, produces a number of factors, such as toxins and adhesins, that are required for full expression of virulence. Filamentous hemagglutinin...
Biet, Franck, Kremer, Laurent, Wolowczuk, Isabelle, Delacre, Myriam, Locht, Camille
Interleukin-18 (IL-18) and IL-12 play a critical role in the expression of cell-mediated immunity involved in host defense against intracellular pathogens. Both cytokines are produced by macrophages...
Overrepresentation of a Gene Family Encoding Extracytoplasmic Solute Receptors in Bordetella
Antoine, Rudy, Jacob-Dubuisson, Françoise, Drobecq, Hervé, Willery, Eve, Lesjean, Sarah, Locht, Camille
A family of genes that are likely to encode extracytoplasmic solute receptors is strongly overrepresented in several β-proteobacteria, including Bordetella pertussis. This gene family, of which...
Immune Response Induced by Recombinant Mycobacterium bovis BCG Producing the Cholera Toxin B Subunit
Biet, Franck, Kremer, Laurent, Wolowczuk, Isabelle, Delacre, Myriam, Locht, Camille
The pentameric form of the cholera toxin B subunit (CTB) is known to be a strong mucosal adjuvant and stimulates antigen-specific secretory immunoglobulin A (IgA) and systemic antibody responses to...
Savine, Evgueni, Warren, Robin M., Van Der Spuy, Gian D., Beyers, Nulda, Van Helden, Paul D., Locht, Camille, ...
Variable number tandem repeats (VNTRs) of elements named mycobacterial interspersed repetitive units (MIRUs) have previously been identified in 12 minisatellite loci of the Mycobacterium tuberculosis...
Tazi, Loubna, El Baghdadi, Jamila, Lesjean, Sarah, Locht, Camille, Supply, Philip, Tibayrenc, Michel, ...
Although lower-resource countries have by far the highest burden of tuberculosis, knowledge of Mycobacterium tuberculosis population structure and genetic diversity in these regions remains almost...
Clantin, Bernard, Hodak, Hélène, Willery, Eve, Locht, Camille, Jacob-Dubuisson, Françoise, Villeret, Vincent
Filamentous hemagglutinin (FHA), the major 230-kDa adhesin of the whooping cough agent Bordetella pertussis, is one of the most efficiently secreted proteins in Gram-negative bacteria. FHA is...
Sun, Yong-Jiang, Bellamy, Richard, Lee, Ann S. G., Ng, Sze Ta, Ravindran, Sindhu, Wong, Sin-Yew, ...
Strain typing using variable-number tandem repeats of mycobacterial interspersed repetitive units (MIRU-VNTR) is a powerful tool for studying the epidemiology and genetic relationships of...
Parra, Marcela, Pickett, Thames, Delogu, Giovanni, Dheenadhayalan, Veerabadran, Debrie, Anne-Sophie, Locht, Camille, ...
The heparin-binding hemagglutinin (HBHA) of Mycobacterium tuberculosis is a surface-expressed adhesin that can affect binding to host cells via a unique, methylated, carboxyl-terminal, lysine-,...
Shamputa, Isdore Chola, Rigouts, Leen, Eyongeta, Lovet Achale, El Aila, Nabil Abdullah, Van Deun, Armand, Salim, Abdul Hamid, ...
Although the heterogeneity of Mycobacterium tuberculosis populations and the existence of mixed infections are now generally accepted, systematic studies on their relative importance are rare. In the...
Shamputa, Isdore Chola, Rigouts, Leen, Eyongeta, Lovet Achale, El Aila, Nabil Abdullah, Van Deun, Armand, Salim, Abdul Hamid, ...
Peirs, Priska, Lefèvre, Philippe, Boarbi, Samira, Wang, Xiao-Ming, Denis, Olivier, Braibant, Martine, ...
By measuring phosphate uptake by Mycobacterium tuberculosis strains with the pstS1 and pstS2 genes genetically inactivated, we showed that these pstS genes encode high-affinity phosphate binding...
Multilocus Variable-Number Tandem Repeat Typing of Mycobacterium ulcerans
Ablordey, Anthony, Swings, Jean, Hubans, Christine, Chemlal, Karim, Locht, Camille, Portaels, Françoise, ...
The apparent genetic homogeneity of Mycobacterium ulcerans contributes to the poorly understood epidemiology of M. ulcerans infection. Here, we report the identification of variable number tandem...
Alonso, Sylvie, Willery, Eve, Renauld-Mongénie, Genevieve, Locht, Camille
Bordetella pertussis, the etiologic agent of whooping cough, is a highly infectious human pathogen capable of inducing mucosal and systemic immune responses upon a single intranasal administration....
Kremer, Laurent, Dover, Lynn G, Carrère, Séverine, Nampoothiri, K Madhavan, Lesjean, Sarah, Brown, Alistair K, ...
Mycolic acids consist of long-chain alpha-alkyl-beta-hydroxy fatty acids that are produced by successive rounds of elongation catalysed by a type II fatty acid synthase (FAS-II). A key feature in the...
Ppm1, a novel polyprenol monophosphomannose synthase from Mycobacterium tuberculosis.
Gurcha, Sudagar S, Baulard, Alain R, Kremer, Laurent, Locht, Camille, Moody, D Branch, Muhlecker, Walter, ...
Dolichol monophosphomannose (DPM) is an ever-present donor of mannose (Man) in various eukaryotic glycosylation processes. Intriguingly, the related polyprenol monophosphomannose (PPM) is involved in...
Van Deutekom, Henk, Supply, Philip, Willery, Eve, Hoijng, Susan P., Locht, Camille, ...
IS6110 fingerprinting of Mycobacterium tuberculosis is the standard identification method in studies on transmission of tuberculosis. However, intensive epidemiological investigation may fail to...
Live Attenuated B. pertussis as a Single-Dose Nasal Vaccine against Whooping Cough
Mielcarek, Nathalie, Debrie, Anne-Sophie, Raze, Dominique, Bertout, Julie, Rouanet, Carine, Younes, Amena Ben, ...
Pertussis is still among the principal causes of death worldwide, and its incidence is increasing even in countries with high vaccine coverage. Although all age groups are susceptible, it is most...
Supply, Philip, Allix, Caroline, Lesjean, Sarah, Cardoso-Oelemann, Mara, Rüsch-Gerdes, Sabine, Willery, Eve, ...
Molecular typing based on 12 loci containing variable numbers of tandem repeats of mycobacterial interspersed repetitive units (MIRU-VNTRs) has been adopted in combination with spoligotyping as the...
Oelemann, Mara Cardoso, Diel, Roland, Vatin, Vincent, Haas, Walter, Rüsch-Gerdes, Sabine, Locht, Camille, ...
An optimized set of 24 mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) loci, including a discriminatory subset of 15 loci, has recently been defined for the...
Heparin-Binding-Hemagglutinin-Induced IFN-γ Release as a Diagnostic Tool for Latent Tuberculosis
Hougardy, Jean-Michel, Schepers, Kinda, Place, Sammy, Drowart, Annie, Lechevin, Véronique, Verscheure, Virginie, ...
Single-Molecule Force Spectroscopy of Mycobacterial Adhesin-Adhesin Interactions▿
Verbelen, Claire, Raze, Dominique, Dewitte, Frédérique, Locht, Camille, Dufrêne, Yves F.
The heparin-binding hemagglutinin (HBHA) is one of the few virulence factors identified for Mycobacterium tuberculosis. It is a surface-associated adhesin that expresses a number of different...
Role of Adhesin Release for Mucosal Colonization by a Bacterial Pathogen
Coutte, Loïc, Alonso, Sylvie, Reveneau, Nathalie, Willery, Eve, Quatannens, Brigitte, Locht, Camille, ...
Pathogen attachment is a crucial early step in mucosal infections. This step is mediated by important virulence factors called adhesins. To exert these functions, adhesins are typically...
Ho, Si Ying, Chua, Shi Qian, Foo, Damian G. W., Locht, Camille, Chow, Vincent T., Poh, Chit Laa, ...
Bordetella pertussis, the causative agent of whooping cough, is a promising and attractive candidate for vaccine delivery via the nasal route, provided that suitable attenuation of this pathogen has...
Origin, Spread and Demography of the Mycobacterium tuberculosis Complex
Wirth, Thierry, Hildebrand, Falk, Allix-Béguec, Caroline, Wölbeling, Florian, Kubica, Tanja, Kremer, Kristin, ...
The evolutionary timing and spread of the Mycobacterium tuberculosis complex (MTBC), one of the most successful groups of bacterial pathogens, remains largely unknown. Here, using mycobacterial...