Blauwkamp, Marsha N, Yu, Jingcheng, Schin, MaryLee A, Burke, Kathleen A, Berry, Marla J, Carlson, Bradley A, ...
Abstract Background Selenoproteins contain selenocysteine (Sec), commonly considered the 21 st genetically encoded amino acid. Many selenoproteins, such as the glutathione peroxidases and thioredoxin...
Loss of housekeeping selenoprotein expression in mouse liver modulates lipoprotein metabolism (2008)
Sengupta, Aniruddha, Carlson, Bradley A., Hoffmann, Victoria J., Gladyshev, Vadim N., Hatfield, Dolph L.
Selenium is incorporated into proteins as selenocysteine (Sec), which is dependent on its specific tRNA, designated tRNA [Ser]Sec. Targeted removal of the tRNA [Ser]Sec gene (Trsp) in mouse...
Xu, Xue-Ming, Carlson, Bradley A., Zhang, Yan, Mix, Heiko, Kryukov, Gregory V., Glass, Richard S., ...
We used comparative genomics and experimental analyses to show that (1) eukaryotes and archaea, which possess the selenocysteine (Sec) protein insertion machinery contain an enzyme,...
Carlson, Bradley A., Moustafa, Mohamed E., Sengupta, Aniruddha, Schweizer, Ulrich, Shrimali, Rajeev, Rao, Mahadev, ...
Novel mouse models were developed in which the hepatic selenoprotein population was targeted for removal by disrupting the selenocysteine (Sec) tRNA [Ser]Sec gene (trsp) and then selenoprotein...
Selenoprotein H Is a Nucleolar Thioredoxin-like Protein with a Unique Expression Pattern (2007)
Novoselov, Sergey V., Kryukov, Gregory V., Xu, Xue-Ming, Carlson, Bradley A., Hatfield, Dolph L., Gladyshev, Vadim N.
The human selenoproteome consists of 25 known selenoproteins, but functions of many of these proteins are not known. Selenoprotein H (SelH) is a recently discovered 14-kDa mammalian protein with no...
Selenophosphate synthetase 2 is essential for selenoprotein biosynthesis (2007)
Xu, Xue-Ming, Carlson, Bradley A., Irons, Robert, Mix, Heiko, Zhong, Nianxin, Gladyshev, Vadim, ...
Selenophosphate synthetase (SelD) generates the selenium donor for selenocysteine biosynthesis in eubacteria. One homologue of SelD in eukaryotes is SPS1 (selenophosphate synthetase 1) and a second...
Biosynthesis of Selenocysteine on Its tRNA in Eukaryotes (2007)
Xu, Xue-Ming, Carlson, Bradley A., Mix, Heiko, Zhang, Yan, Saira, Kazima, Glass, Richard S., ...
Selenocysteine (Sec) is cotranslationally inserted into protein in response to UGA codons and is the 21st amino acid in the genetic code. However, the means by which Sec is synthesized in eukaryotes...
Biosynthesis of Selenocysteine on Its tRNA in Eukaryotes (2007)
Xue-Ming Xu, Bradley A. Carlson, Heiko Mix, Yan Zhang, Kazima Saira, Richard S. Glass, ...
Selenocysteine (Sec) is cotranslationally inserted into protein in response to UGA codons and is the 21st amino acid in the genetic code. However, the means by which Sec is synthesized in eukaryotes...
Mouse models for assessing the role of selenoproteins in health and development (2006)
Carlson, Bradley A., Xu, Xue-Ming, Shrimali, Rajeev, Sengupta, Aniruddha, Yoo, Min-Hyuk, Zhong, Nianxin, ...
Mouse models have been generated to assess the roles of selenoproteins involved with housekeeping tasks and/or stress-related phenomena in development and health. Each mouse model has taken advantage...
Salinas, Gustavo, Romero, Héctor, Xu, Xue-Ming, Carlson, Bradley A., Hatfield, Dolph L., Gladyshev, Vadim N.
The complete sequencing of genomes and the development of in silico methods for identification of genes encoding selenocysteine (Sec)-containing proteins have greatly contributed to shape our view on...
Mammalian and other eukaryotic selenocysteine tRNAs (2006)
Carlson, Bradley A., Xu, Xue-Ming, Shrimali, Rajeev, Sengupta, Aniruddha, Yoo, Min-Hyuk, Irons, Robert, ...
Selenocysteine (Sec) tRNA occupies a prominent position in the expression of selenoproteins as it is essential for their synthesis and it provides the means by which selenium is co-translationally...
Yoo, Min-Hyuk, Xu, Xue-Ming, Carlson, Bradley A., Gladyshev, Vadim N., Hatfield, Dolph L.
Dietary selenium has potent cancer prevention activity. Both low molecular weight selenocompounds and selenoproteins are implicated in this effect. Thioredoxin reductase 1 (TR1) is one of the major...
Xu, Xue-Ming, Mix, Heiko, Carlson, Bradley A., Grabowski, Paula J., Gladyshev, Vadim N., Berry, Marla J., ...
Selenocysteine (Sec) is inserted into selenoproteins co-translationally with the help of various cis- and trans-acting factors. The specific mechanisms of Sec biosynthesis and insertion into protein...
Carlson, Bradley A., Xu, Xue-Ming, Gladyshev, Vadim N., Hatfield, Dolph L.
Selenium is an essential micronutrient in the diet of mammals and has many health benefits. Selenium-containing proteins are responsible for most, if not all, of these benefits. This element is...
Carlson, Bradley A., Xu, Xue-Ming, Gladyshev, Vadim N., Hatfield, Dolph L.
Selenocysteine (Sec) is the 21st amino acid in the genetic code. Its tRNA is variably methylated on the 2'-Ohydroxyl site of the ribosyl moiety at position 34 (Um34). Herein, we identified a role of...
Identification and characterization of phosphoseryl-tRNA [Ser]Sec kinase (2004)
Carlson, Bradley A., Xu, Xue-Ming, Kryukov, Gregory V., Rao, Mahadev, Berry, Marla J., Gladyshev, Vadim N., ...
In 1970, a kinase activity that phosphorylated a minor species of seryl-tRNA to form phosphoseryl-tRNA was found in rooster liver [Maenpaa, P. H. & Bernfield, M. R. (1970) Proc. Natl. Acad. Sci. USA...
Carlson, Bradley A., Novoselov, Sergey V., Kumaraswamy, Easwari, Jae Lee, Byeong, Anver, Miriam R., Gladyshev, Vadim N., ...
Selenium is essential in mammalian embryonic development. However, in adults, selenoprotein levels in several organs including liver can be substantially reduced by selenium deficiency without any...
Kwon, So Yeon, Badenhorst, Paul, Martin-Romero, F. Javier, Carlson, Bradley A., Paterson, Bruce M., Gladyshev, Vadim, ...
Selenium is implicated in many diseases, including cancer, but its function at the molecular level is poorly understood. BthD is one of three selenoproteins recently identified in Drosophila. To...
Chlamydomonas reinhardtii selenocysteine tRNA [Ser]Sec (2003)
Rao, Mahadev, Carlson, Bradley A., Novoselov, Sergey V., Weeks, Donald P., Gladyshev, Vadim, Hatfield, Dolph L.
Eukaryotic selenocysteine (Sec) protein insertion machinery was thought to be restricted to animals, but the occurrence of both Sec-containing proteins and the Sec insertion system was recently found...
Selective Removal of the Selenocysteine tRNA [Ser]Sec Gene (Trsp) in Mouse Mammary Epithelium (2003)
Kumaraswamy, Easwari, Carlson, Bradley A., Morgan, Fanta, Miyoshi, Keiko, Robinson, Gertraud W., Su, Dan, ...
Mice homozygous for an allele encoding the selenocysteine (Sec) tRNA [Ser]Sec gene (Trsp) flanked by loxP sites were generated. Cre recombinase-dependent removal of Trsp in these mice was lethal to...
Selenium Metabolism in Drosophila (2001)
Martin-Romero, F. Javier, Kryukov, Gregory V., Lobanov, Alexei V., Carlson, Bradley A., Lee, Byeong Jae, Gladyshev, Vadim N., ...
Selenocysteine is a rare amino acid in protein that is encoded by UGA with the requirement of a downstream mRNA stem-loop structure, the selenocysteine insertion sequence element. To detect...
Moustafa, Mohamed E., Carlson, Bradley A., El-Saadani, Muhammed A., Kryukov, Gregory V., Sun, Qi-An, Harney, John W., ...
Selenocysteine (Sec) tRNA (tRNA [Ser]Sec) serves as both the site of Sec biosynthesis and the adapter molecule for donation of this amino acid to protein. The consequences on selenoprotein...
Moustafa, Mohamed E., Carlson, Bradley A., El-Saadani, Muhammad A., Kryukov, Gregory V., Sun, Qi-An, Harney, John W., ...
Selenocysteine (Sec) tRNA (tRNA[Ser]Sec) serves as both the site of Sec biosynthesis and the adapter molecule for donation of this amino acid to protein. The consequences on selenoprotein...
Dettenhofer, Markus, Cen, Shan, Carlson, Bradley A., Kleiman, Lawrence, Yu, Xiao-Fang
The vif gene of human immunodeficiency virus type 1 (HIV-1) is essential for viral replication, although the functional target of Vif remains elusive. HIV-1 vif mutant virions derived from...
Selective Removal of the Selenocysteine tRNA[Ser]Sec Gene (Trsp) in Mouse Mammary Epithelium
Kumaraswamy, Easwari, Carlson, Bradley A., Morgan, Fanta, Miyoshi, Keiko, Robinson, Gertraud W., Su, Dan, ...
Mice homozygous for an allele encoding the selenocysteine (Sec) tRNA[Ser]Sec gene (Trsp) flanked by loxP sites were generated. Cre recombinase-dependent removal of Trsp in these mice was lethal to...
Kwon, So Yeon, Badenhorst, Paul, Martin-Romero, F. Javier, Carlson, Bradley A., Paterson, Bruce M., Gladyshev, Vadim N., ...
Selenium is implicated in many diseases, including cancer, but its function at the molecular level is poorly understood. BthD is one of three selenoproteins recently identified in Drosophila. To...
A novel RNA binding protein, SBP2, is required for the translation of mammalian selenoprotein mRNAs
Copeland, Paul R., Fletcher, Julia E., Carlson, Bradley A., Hatfield, Dolph L., Driscoll, Donna M.
In eukaryotes, the decoding of the UGA codon as selenocysteine (Sec) requires a Sec insertion sequence (SECIS) element in the 3′ untranslated region of the mRNA. We purified a SECIS binding...
Anderson, James, Phan, Lon, Cuesta, Rafael, Carlson, Bradley A., Pak, Marie, Asano, Katsura, ...
Gcd10p and Gcd14p are essential proteins required for the initiation of protein synthesis and translational repression of GCN4 mRNA. The phenotypes of gcd10 mutants were suppressed by...
Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase
Carlson, Bradley A., Xu, Xue-Ming, Kryukov, Gregory V., Rao, Mahadev, Berry, Marla J., Gladyshev, Vadim N., ...
In 1970, a kinase activity that phosphorylated a minor species of seryl-tRNA to form phosphoseryl-tRNA was found in rooster liver [Maenpaa, P. H. & Bernfield, M. R. (1970) Proc. Natl. Acad. Sci. USA...
Decoding apparatus for eukaryotic selenocysteine insertion
Tujebajeva, Rosa M., Copeland, Paul R., Xu, Xue-Ming, Carlson, Bradley A., Harney, John W., Driscoll, Donna M., ...
Decoding UGA as selenocysteine requires a unique tRNA, a specialized elongation factor, and specific secondary structures in the mRNA, termed SECIS elements. Eukaryotic SECIS elements are found in...
Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec
RAO, MAHADEV, CARLSON, BRADLEY A., NOVOSELOV, SERGEY V., WEEKS, DONALD P., GLADYSHEV, VADIM N., HATFIELD, DOLPH L.
Eukaryotic selenocysteine (Sec) protein insertion machinery was thought to be restricted to animals, but the occurrence of both Sec-containing proteins and the Sec insertion system was recently found...
Supramolecular Complexes Mediate Selenocysteine Incorporation In Vivo
Small-Howard, Andrea, Morozova, Nadya, Stoytcheva, Zoia, Forry, Erin P., Mansell, John B., Harney, John W., ...
Selenocysteine incorporation in eukaryotes occurs cotranslationally at UGA codons via the interactions of RNA-protein complexes, one comprised of selenocysteyl (Sec)-tRNA[Ser]Sec and its specific...
Moustafa, Mohamed E., Carlson, Bradley A., El-Saadani, Muhammad A., Kryukov, Gregory V., Sun, Qi-An, Harney, John W., ...
Selenocysteine (Sec) tRNA (tRNA[Ser]Sec) serves as both the site of Sec biosynthesis and the adapter molecule for donation of this amino acid to protein. The consequences on selenoprotein...
Dettenhofer, Markus, Cen, Shan, Carlson, Bradley A., Kleiman, Lawrence, Yu, Xiao-Fang
The vif gene of human immunodeficiency virus type 1 (HIV-1) is essential for viral replication, although the functional target of Vif remains elusive. HIV-1 vif mutant virions derived from...
Selective Removal of the Selenocysteine tRNA[Ser]Sec Gene (Trsp) in Mouse Mammary Epithelium
Kumaraswamy, Easwari, Carlson, Bradley A., Morgan, Fanta, Miyoshi, Keiko, Robinson, Gertraud W., Su, Dan, ...
Mice homozygous for an allele encoding the selenocysteine (Sec) tRNA[Ser]Sec gene (Trsp) flanked by loxP sites were generated. Cre recombinase-dependent removal of Trsp in these mice was lethal to...
Kwon, So Yeon, Badenhorst, Paul, Martin-Romero, F. Javier, Carlson, Bradley A., Paterson, Bruce M., Gladyshev, Vadim N., ...
Selenium is implicated in many diseases, including cancer, but its function at the molecular level is poorly understood. BthD is one of three selenoproteins recently identified in Drosophila. To...
A novel RNA binding protein, SBP2, is required for the translation of mammalian selenoprotein mRNAs
Copeland, Paul R., Fletcher, Julia E., Carlson, Bradley A., Hatfield, Dolph L., Driscoll, Donna M.
In eukaryotes, the decoding of the UGA codon as selenocysteine (Sec) requires a Sec insertion sequence (SECIS) element in the 3′ untranslated region of the mRNA. We purified a SECIS binding...
Anderson, James, Phan, Lon, Cuesta, Rafael, Carlson, Bradley A., Pak, Marie, Asano, Katsura, ...
Gcd10p and Gcd14p are essential proteins required for the initiation of protein synthesis and translational repression of GCN4 mRNA. The phenotypes of gcd10 mutants were suppressed by...
Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase
Carlson, Bradley A., Xu, Xue-Ming, Kryukov, Gregory V., Rao, Mahadev, Berry, Marla J., Gladyshev, Vadim N., ...
In 1970, a kinase activity that phosphorylated a minor species of seryl-tRNA to form phosphoseryl-tRNA was found in rooster liver [Maenpaa, P. H. & Bernfield, M. R. (1970) Proc. Natl. Acad. Sci. USA...
Decoding apparatus for eukaryotic selenocysteine insertion
Tujebajeva, Rosa M., Copeland, Paul R., Xu, Xue-Ming, Carlson, Bradley A., Harney, John W., Driscoll, Donna M., ...
Decoding UGA as selenocysteine requires a unique tRNA, a specialized elongation factor, and specific secondary structures in the mRNA, termed SECIS elements. Eukaryotic SECIS elements are found in...
Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec
RAO, MAHADEV, CARLSON, BRADLEY A., NOVOSELOV, SERGEY V., WEEKS, DONALD P., GLADYSHEV, VADIM N., HATFIELD, DOLPH L.
Eukaryotic selenocysteine (Sec) protein insertion machinery was thought to be restricted to animals, but the occurrence of both Sec-containing proteins and the Sec insertion system was recently found...
Supramolecular Complexes Mediate Selenocysteine Incorporation In Vivo
Small-Howard, Andrea, Morozova, Nadya, Stoytcheva, Zoia, Forry, Erin P., Mansell, John B., Harney, John W., ...
Selenocysteine incorporation in eukaryotes occurs cotranslationally at UGA codons via the interactions of RNA-protein complexes, one comprised of selenocysteyl (Sec)-tRNA[Ser]Sec and its specific...
Biosynthesis of Selenocysteine on Its tRNA in Eukaryotes
Xu, Xue-Ming, Carlson, Bradley A, Mix, Heiko, Zhang, Yan, Saira, Kazima, Glass, Richard S, ...
Selenocysteine (Sec) is cotranslationally inserted into protein in response to UGA codons and is the 21st amino acid in the genetic code. However, the means by which Sec is synthesized in eukaryotes...
Yoo, Min-Hyuk, Xu, Xue-Ming, Carlson, Bradley A., Patterson, Andrew D., Gladyshev, Vadim N., Hatfield, Dolph L.
Thioredoxin reductase 1 (TR1) is a major redox regulator in mammalian cells. As an important antioxidant selenoprotein, TR1 is thought to participate in cancer prevention, but is also known to be...
A recoding element that stimulates decoding of UGA codons by Sec tRNA[Ser]Sec
Howard, Michael T., Moyle, Mark W., Aggarwal, Gaurav, Carlson, Bradley A., Anderson, Christine B.
Selenocysteine insertion during decoding of eukaryotic selenoprotein mRNA requires several trans-acting factors and a cis-acting selenocysteine insertion sequence (SECIS) usually located in the 3′...
A new strategy for assessing selenoprotein function: siRNA knockdown/knock-in targeting the 3′-UTR
Yoo, Min-Hyuk, Xu, Xue-Ming, Turanov, Anton A., Carlson, Bradley A., Gladyshev, Vadim N., Hatfield, Dolph L.
Selenocysteine insertion into protein in mammalian cells requires RNA elements in the 3′-untranslated regions (3′-UTRs) of selenoprotein genes. The occurrence of these conserved sequences should...