Novel metabolism in Chlamydomonas through the lens of genomics (2007)
Grossman, Arthur R., Croft, Martin, Gladyshev, Vadim N., Merchant, Sebeeha S., Posewitz, Matthew C., Prochnik, Simon, ...
Chlamydomonas has traditionally been exploited as an organism that is associated with sophisticated physiological, genetic and molecular analyses, all of which have been used to elucidate several...
Bhaya, Devaki, Watanabe, Natsu, Ogawa, Teruo, Grossman, Arthur R.
Disruption of a gene for an alternative sigma factor, designated sigF, in the freshwater, unicellular cyanobacterium Synechocystis sp. strain PCC6803 resulted in a pleiotropic phenotype. Most...
Psr1, a nuclear localized protein that regulates phosphorus metabolism in Chlamydomonas
Wykoff, Dennis D., Grossman, Arthur R., Weeks, Donald P., Usuda, Hideaki, Shimogawara, Kosuke
Understanding the ways in which phosphorus metabolism is regulated in photosynthetic eukaryotes is critical for optimizing crop productivity and managing aquatic ecosystems in which phosphorus can be...
Schwarz, Rakefet, Grossman, Arthur R.
Microorganisms must sense their environment and rapidly tune their metabolism to ambient conditions to efficiently use available resources. We have identified a gene encoding a response regulator,...
The roles of specific xanthophylls in photoprotection
Niyogi, Krishna K., Björkman, Olle, Grossman, Arthur R.
Xanthophyll pigments have critical structural and functional roles in the photosynthetic light-harvesting complexes of algae and vascular plants. Genetic dissection of xanthophyll metabolism in the...
Highly expressed and alien genes of the Synechocystis genome
Mrázek, Jan, Bhaya, Devaki, Grossman, Arthur R., Karlin, Samuel
Comparisons of codon frequencies of genes to several gene classes are used to characterize highly expressed and alien genes on the Synechocystis PCC6803 genome. The primary gene classes include the...
Bhaya, Devaki, Takahashi, Akiko, Grossman, Arthur R.
To optimize photosynthesis, cyanobacteria move toward or away from a light source by a process known as phototaxis. Phototactic movement of the cyanobacterium Synechocystis PCC6803 is a...
Wykoff, Dennis D., Davies, John P., Melis, Anastasios, Grossman, Arthur R.
The light-saturated rate of photosynthetic O2 evolution in Chlamydomonas reinhardtii declined by approximately 75% on a per-cell basis after 4 d of P starvation or 1 d of S starvation. Quantitation...
Chlamydomonas reinhardtii Mutants Abnormal in Their Responses to Phosphorus Deprivation1
Shimogawara, Kosuke, Wykoff, Dennis D., Usuda, Hideaki, Grossman, Arthur R.
P-starved plants scavenge inorganic phosphate (Pi) by developing elevated rates of Pi uptake, synthesizing extracellular phosphatases, and secreting organic acids. To elucidate mechanisms controlling...
Dolganov, Nadia, Grossman, Arthur R.
To optimize the utilization of photosynthate and avoid damage that can result from the absorption of excess excitation energy, photosynthetic organisms must rapidly modify the synthesis and...
Bhaya, Devaki, Vaulot, Daniel, Amin, Pinky, Takahashi, Akiko Watanabe, Grossman, Arthur R.
Global identification of differentially regulated genes in prokaryotes is constrained because the mRNA does not have a 3′ polyadenylation extension; this precludes specific separation of mRNA from...
A Gene of Synechocystis sp. Strain PCC 6803 Encoding a Novel Iron Transporter
Katoh, Hirokazu, Grossman, Arthur R., Hagino, Natsu, Ogawa, Teruo
A mutant of Synechocystis sp. strain PCC 6803 disrupted for sll1878 exhibited greatly reduced Fe3+ transport activity. The Km value of sll1878-dependent Fe3+ transport in cells grown in iron-replete...
Genes Essential to Iron Transport in the Cyanobacterium Synechocystis sp. Strain PCC 6803
Katoh, Hirokazu, Hagino, Natsu, Grossman, Arthur R., Ogawa, Teruo
Genes encoding polypeptides of an ATP binding cassette (ABC)-type ferric iron transporter that plays a major role in iron acquisition in Synechocystis sp. strain PCC 6803 were identified. These genes...
Bhaya, Devaki, Takahashi, Akiko, Shahi, Payam, Grossman, Arthur R.
We screened for transposon-generated mutants of Synechocystis sp. strain PCC 6803 that exhibited aberrant phototactic movement. Of the 300 mutants generated, about 50 have been partially...
Sulfur Economy and Cell Wall Biosynthesis during Sulfur Limitation of Chlamydomonas reinhardtii1
Takahashi, Hideki, Braby, Caren E., Grossman, Arthur R.
We have identified two novel periplasmic/cell wall polypeptides that specifically accumulate during sulfur limitation of Chlamydomonas reinhardtii. These polypeptides, present at high levels in the...
Van Waasbergen, Lorraine G., Dolganov, Nadia, Grossman, Arthur R.
The HliA protein of the cyanobacterium Synechococcus elongatus PCC 7942 is a small, thylakoid-associated protein that appears to play a role in photoprotection; its transcript rapidly accumulates in...
Multiple Light Inputs Control Phototaxis in Synechocystis sp. Strain PCC6803†
Ng, Wing-On, Grossman, Arthur R., Bhaya, Devaki
The phototactic behavior of individual cells of the cyanobacterium Synechocystis sp. strain PCC6803 was studied with a glass slide-based phototaxis assay. Data from fluence rate-response curves and...
A Major Light-Harvesting Polypeptide of Photosystem II Functions in Thermal DissipationW⃞
Elrad, Dafna, Niyogi, Krishna K., Grossman, Arthur R.
Under high-light conditions, photoprotective mechanisms minimize the damaging effects of excess light. A primary photoprotective mechanism is thermal dissipation of excess excitation energy within...
Chlamydomonas reinhardtii Genome Project. A Guide to the Generation and Use of the cDNA Information1
Shrager, Jeff, Hauser, Charles, Chang, Chiung-Wen, Harris, Elizabeth H., Davies, John, McDermott, Jeff, ...
The National Science Foundation-funded Chlamydomonas reinhardtii genome project involves (a) construction and sequencing of cDNAs isolated from cells exposed to various environmental conditions, (b)...
Chlamydomonas reinhardtii at the Crossroads of Genomics†
Grossman, Arthur R., Harris, Elizabeth E., Hauser, Charles, Lefebvre, Paul A., Martinez, Diego, Rokhsar, Dan, ...
Isolation and characterization of calmodulin from spinach leaves and in vitro translation mixtures
Eldik, Linda J. Van, Grossman, Arthur R., Iverson, David B., Watterson, D. Martin
Calmodulin, a multifunctional calcium-modulated protein, has been isolated from spinach leaf tissue and from spinach leaf messenger RNA translation products. The translation protein and the spinach...
Biosynthesis of carbonic anhydrase in Chlamydomonas reinhardtii during adaptation to low CO2
Coleman, John R., Grossman, Arthur R.
The unicellular green alga Chlamydomonas reinhardtii synthesizes carbonic anhydrase in response to low levels of CO2 (i.e., air levels of CO2). This enzyme, localized predominantly in the periplasmic...
Hsiao, Hui-Yi, He, Qingfang, Van Waasbergen, Lorraine G., Grossman, Arthur R.
We have deleted a gene for a sensor histidine kinase, dspA (or hik33), in the cyanobacterium Synechocystis sp. strain PCC6803. In low and moderate light, the mutant grew slowly under photoautotrophic...
Consequences of a Deletion in dspA on Transcript Accumulation in Synechocystis sp. Strain PCC6803†
Tu, Chao-Jung, Shrager, Jeffrey, Burnap, Robert L., Postier, Bradley L., Grossman, Arthur R.
A sensor histidine kinase of Synechococcus sp. strain PCC7942, designated nblS, was previously identified and shown to be critical for the acclimation of cells to high-light and nutrient limitation...
Zhang, Zhaoduo, Shrager, Jeff, Jain, Monica, Chang, Chiung-Wen, Vallon, Olivier, Grossman, Arthur R.
Responses of photosynthetic organisms to sulfur starvation include (i) increasing the capacity of the cell for transporting and/or assimilating exogenous sulfate, (ii) restructuring cellular features...
Montgomery, Beronda L., Casey, Elena Silva, Grossman, Arthur R., Kehoe, David M.
All known phycobiliproteins have light-harvesting roles during photosynthesis and are found in water-soluble phycobilisomes, the light-harvesting complexes of cyanobacteria, cyanelles, and red algae....
Identification and Purification of a Derepressible Alkaline Phosphatase from Anacystis nidulans R2 1
Block, Maryse A., Grossman, Arthur R.
We have examined the increase in alkaline phosphatase activity in the cyanobacterium Anacystis nidulans R2 upon phosphate deprivation. Much of the activity is released into the medium when A....
Identification of Extracellular Carbonic Anhydrase of Chlamydomonas reinhardtii1
Coleman, John R., Berry, Joseph A., Togasaki, Robert K., Grossman, Arthur R.
We have examined the induction of carbonic anhydrase activity in Chlamydomonas reinhardtii and have identified the polypeptide responsible for this activity. This polypeptide was not synthesized when...
Fawley, Marvin W., Grossman, Arthur R.
A light-harvesting fucoxanthin-chlorophyll a/c-protein complex has been isolated from the diatom Phaeodactylum tricornutum by detergent extraction of thylakoid membranes coupled with sucrose density...
Moseley, Jeffrey L., Chang, Chiung-Wen, Grossman, Arthur R.
The Chlamydomonas reinhardtii transcription factor PSR1 is required for the control of activities involved in scavenging phosphate from the environment during periods of phosphorus limitation....
Chang, Chiung-Wen, Moseley, Jeffrey L., Wykoff, Dennis, Grossman, Arthur R.
Organisms exhibit a diverse set of responses when exposed to low-phosphate conditions. Some of these responses are specific for phosphorus limitation, including responses that enable cells to...
Genome-Based Examination of Chlorophyll and Carotenoid Biosynthesis in Chlamydomonas reinhardtii1[w]
Lohr, Martin, Im, Chung-Soon, Grossman, Arthur R.
The unicellular green alga Chlamydomonas reinhardtii is a particularly important model organism for the study of photosynthesis since this alga can grow heterotrophically, and mutants in...
Steunou, Anne-Soisig, Bhaya, Devaki, Bateson, Mary M., Melendrez, Melanie C., Ward, David M., Brecht, Eric, ...
Genome sequences of two Synechococcus ecotypes inhabiting the Octopus Spring microbial mat in Yellowstone National Park revealed the presence of all genes required for nitrogenase biosynthesis. We...
Bhaya, Devaki, Watanabe, Natsu, Ogawa, Teruo, Grossman, Arthur R.
Disruption of a gene for an alternative sigma factor, designated sigF, in the freshwater, unicellular cyanobacterium Synechocystis sp. strain PCC6803 resulted in a pleiotropic phenotype. Most...
Psr1, a nuclear localized protein that regulates phosphorus metabolism in Chlamydomonas
Wykoff, Dennis D., Grossman, Arthur R., Weeks, Donald P., Usuda, Hideaki, Shimogawara, Kosuke
Understanding the ways in which phosphorus metabolism is regulated in photosynthetic eukaryotes is critical for optimizing crop productivity and managing aquatic ecosystems in which phosphorus can be...
Schwarz, Rakefet, Grossman, Arthur R.
Microorganisms must sense their environment and rapidly tune their metabolism to ambient conditions to efficiently use available resources. We have identified a gene encoding a response regulator,...
The roles of specific xanthophylls in photoprotection
Niyogi, Krishna K., Björkman, Olle, Grossman, Arthur R.
Xanthophyll pigments have critical structural and functional roles in the photosynthetic light-harvesting complexes of algae and vascular plants. Genetic dissection of xanthophyll metabolism in the...
Highly expressed and alien genes of the Synechocystis genome
Mrázek, Jan, Bhaya, Devaki, Grossman, Arthur R., Karlin, Samuel
Comparisons of codon frequencies of genes to several gene classes are used to characterize highly expressed and alien genes on the Synechocystis PCC6803 genome. The primary gene classes include the...
Bhaya, Devaki, Takahashi, Akiko, Grossman, Arthur R.
To optimize photosynthesis, cyanobacteria move toward or away from a light source by a process known as phototaxis. Phototactic movement of the cyanobacterium Synechocystis PCC6803 is a...
Wykoff, Dennis D., Davies, John P., Melis, Anastasios, Grossman, Arthur R.
The light-saturated rate of photosynthetic O2 evolution in Chlamydomonas reinhardtii declined by approximately 75% on a per-cell basis after 4 d of P starvation or 1 d of S starvation. Quantitation...
Chlamydomonas reinhardtii Mutants Abnormal in Their Responses to Phosphorus Deprivation1
Shimogawara, Kosuke, Wykoff, Dennis D., Usuda, Hideaki, Grossman, Arthur R.
P-starved plants scavenge inorganic phosphate (Pi) by developing elevated rates of Pi uptake, synthesizing extracellular phosphatases, and secreting organic acids. To elucidate mechanisms controlling...
Dolganov, Nadia, Grossman, Arthur R.
To optimize the utilization of photosynthate and avoid damage that can result from the absorption of excess excitation energy, photosynthetic organisms must rapidly modify the synthesis and...
Bhaya, Devaki, Vaulot, Daniel, Amin, Pinky, Takahashi, Akiko Watanabe, Grossman, Arthur R.
Global identification of differentially regulated genes in prokaryotes is constrained because the mRNA does not have a 3′ polyadenylation extension; this precludes specific separation of mRNA from...
A Gene of Synechocystis sp. Strain PCC 6803 Encoding a Novel Iron Transporter
Katoh, Hirokazu, Grossman, Arthur R., Hagino, Natsu, Ogawa, Teruo
A mutant of Synechocystis sp. strain PCC 6803 disrupted for sll1878 exhibited greatly reduced Fe3+ transport activity. The Km value of sll1878-dependent Fe3+ transport in cells grown in iron-replete...
Genes Essential to Iron Transport in the Cyanobacterium Synechocystis sp. Strain PCC 6803
Katoh, Hirokazu, Hagino, Natsu, Grossman, Arthur R., Ogawa, Teruo
Genes encoding polypeptides of an ATP binding cassette (ABC)-type ferric iron transporter that plays a major role in iron acquisition in Synechocystis sp. strain PCC 6803 were identified. These genes...
Bhaya, Devaki, Takahashi, Akiko, Shahi, Payam, Grossman, Arthur R.
We screened for transposon-generated mutants of Synechocystis sp. strain PCC 6803 that exhibited aberrant phototactic movement. Of the 300 mutants generated, about 50 have been partially...
Sulfur Economy and Cell Wall Biosynthesis during Sulfur Limitation of Chlamydomonas reinhardtii1
Takahashi, Hideki, Braby, Caren E., Grossman, Arthur R.
We have identified two novel periplasmic/cell wall polypeptides that specifically accumulate during sulfur limitation of Chlamydomonas reinhardtii. These polypeptides, present at high levels in the...
Van Waasbergen, Lorraine G., Dolganov, Nadia, Grossman, Arthur R.
The HliA protein of the cyanobacterium Synechococcus elongatus PCC 7942 is a small, thylakoid-associated protein that appears to play a role in photoprotection; its transcript rapidly accumulates in...
Multiple Light Inputs Control Phototaxis in Synechocystis sp. Strain PCC6803†
Ng, Wing-On, Grossman, Arthur R., Bhaya, Devaki
The phototactic behavior of individual cells of the cyanobacterium Synechocystis sp. strain PCC6803 was studied with a glass slide-based phototaxis assay. Data from fluence rate-response curves and...
A Major Light-Harvesting Polypeptide of Photosystem II Functions in Thermal DissipationW⃞
Elrad, Dafna, Niyogi, Krishna K., Grossman, Arthur R.
Under high-light conditions, photoprotective mechanisms minimize the damaging effects of excess light. A primary photoprotective mechanism is thermal dissipation of excess excitation energy within...
Chlamydomonas reinhardtii Genome Project. A Guide to the Generation and Use of the cDNA Information1
Shrager, Jeff, Hauser, Charles, Chang, Chiung-Wen, Harris, Elizabeth H., Davies, John, McDermott, Jeff, ...
The National Science Foundation-funded Chlamydomonas reinhardtii genome project involves (a) construction and sequencing of cDNAs isolated from cells exposed to various environmental conditions, (b)...
Chlamydomonas reinhardtii at the Crossroads of Genomics†
Grossman, Arthur R., Harris, Elizabeth E., Hauser, Charles, Lefebvre, Paul A., Martinez, Diego, Rokhsar, Dan, ...
Isolation and characterization of calmodulin from spinach leaves and in vitro translation mixtures
Eldik, Linda J. Van, Grossman, Arthur R., Iverson, David B., Watterson, D. Martin
Calmodulin, a multifunctional calcium-modulated protein, has been isolated from spinach leaf tissue and from spinach leaf messenger RNA translation products. The translation protein and the spinach...
Biosynthesis of carbonic anhydrase in Chlamydomonas reinhardtii during adaptation to low CO2
Coleman, John R., Grossman, Arthur R.
The unicellular green alga Chlamydomonas reinhardtii synthesizes carbonic anhydrase in response to low levels of CO2 (i.e., air levels of CO2). This enzyme, localized predominantly in the periplasmic...
Hsiao, Hui-Yi, He, Qingfang, Van Waasbergen, Lorraine G., Grossman, Arthur R.
We have deleted a gene for a sensor histidine kinase, dspA (or hik33), in the cyanobacterium Synechocystis sp. strain PCC6803. In low and moderate light, the mutant grew slowly under photoautotrophic...
Consequences of a Deletion in dspA on Transcript Accumulation in Synechocystis sp. Strain PCC6803†
Tu, Chao-Jung, Shrager, Jeffrey, Burnap, Robert L., Postier, Bradley L., Grossman, Arthur R.
A sensor histidine kinase of Synechococcus sp. strain PCC7942, designated nblS, was previously identified and shown to be critical for the acclimation of cells to high-light and nutrient limitation...
Zhang, Zhaoduo, Shrager, Jeff, Jain, Monica, Chang, Chiung-Wen, Vallon, Olivier, Grossman, Arthur R.
Responses of photosynthetic organisms to sulfur starvation include (i) increasing the capacity of the cell for transporting and/or assimilating exogenous sulfate, (ii) restructuring cellular features...
Montgomery, Beronda L., Casey, Elena Silva, Grossman, Arthur R., Kehoe, David M.
All known phycobiliproteins have light-harvesting roles during photosynthesis and are found in water-soluble phycobilisomes, the light-harvesting complexes of cyanobacteria, cyanelles, and red algae....
Identification and Purification of a Derepressible Alkaline Phosphatase from Anacystis nidulans R2 1
Block, Maryse A., Grossman, Arthur R.
We have examined the increase in alkaline phosphatase activity in the cyanobacterium Anacystis nidulans R2 upon phosphate deprivation. Much of the activity is released into the medium when A....
Identification of Extracellular Carbonic Anhydrase of Chlamydomonas reinhardtii1
Coleman, John R., Berry, Joseph A., Togasaki, Robert K., Grossman, Arthur R.
We have examined the induction of carbonic anhydrase activity in Chlamydomonas reinhardtii and have identified the polypeptide responsible for this activity. This polypeptide was not synthesized when...
Fawley, Marvin W., Grossman, Arthur R.
A light-harvesting fucoxanthin-chlorophyll a/c-protein complex has been isolated from the diatom Phaeodactylum tricornutum by detergent extraction of thylakoid membranes coupled with sucrose density...
Chang, Chiung-Wen, Moseley, Jeffrey L., Wykoff, Dennis, Grossman, Arthur R.
Organisms exhibit a diverse set of responses when exposed to low-phosphate conditions. Some of these responses are specific for phosphorus limitation, including responses that enable cells to...
Genome-Based Examination of Chlorophyll and Carotenoid Biosynthesis in Chlamydomonas reinhardtii1[w]
Lohr, Martin, Im, Chung-Soon, Grossman, Arthur R.
The unicellular green alga Chlamydomonas reinhardtii is a particularly important model organism for the study of photosynthesis since this alga can grow heterotrophically, and mutants in...
Moseley, Jeffrey L., Chang, Chiung-Wen, Grossman, Arthur R.
The Chlamydomonas reinhardtii transcription factor PSR1 is required for the control of activities involved in scavenging phosphate from the environment during periods of phosphorus limitation....
Steunou, Anne-Soisig, Bhaya, Devaki, Bateson, Mary M., Melendrez, Melanie C., Ward, David M., Brecht, Eric, ...
Genome sequences of two Synechococcus ecotypes inhabiting the Octopus Spring microbial mat in Yellowstone National Park revealed the presence of all genes required for nitrogenase biosynthesis. We...
Jain, Monica, Shrager, Jeff, Harris, Elizabeth H., Halbrook, Renee, Grossman, Arthur R., Hauser, Charles, ...
Clustering and assembly of expressed sequence tags (ESTs) constitute the basis for most genomewide descriptions of a transcriptome. This approach is limited by the decline in sequence quality toward...
Responses of a Thermophilic Synechococcus Isolate from the Microbial Mat of Octopus Spring to Light▿
Kilian, Oliver, Steunou, Anne-Soisig, Fazeli, Fariba, Bailey, Shaun, Bhaya, Devaki, Grossman, Arthur R.
Thermophilic cyanobacteria of the genus Synechococcus are major contributors to photosynthetic carbon fixation in the photic zone of microbial mats in Octopus Spring, Yellowstone National Park....