André Sentenac

A Subunit of Yeast TFIIIC Participates in the Recruitment of TATA-Binding Protein

Deprez, Eric, Arrebola, Rosalía, Conesa, Christine, Sentenac, André

TFIIIC plays a key role in nucleating the assembly of the initiation factor TFIIIB on class III genes. We have characterized an essential gene, TFC8, encoding the 60-kDa polypeptide, τ60, present in...

A Novel Subunit of Yeast RNA Polymerase III Interacts with the TFIIB-Related Domain of TFIIIB70

Ferri, Maria-Laura, Peyroche, Gérald, Siaut, Magali, Lefebvre, Olivier, Carles, Christophe, Conesa, Christine, ...

There is limited information on how eukaryotic RNA polymerases (Pol) recognize their cognate preinitiation complex. We have characterized a polypeptide copurifying with yeast Pol III. This protein,...

High-Mobility-Group Proteins NHP6A and NHP6B Participate in Activation of the RNA Polymerase III SNR6 Gene

Lopez, Sébastien, Livingstone-Zatchej, Magda, Jourdain, Sabine, Thoma, Fritz, Sentenac, André, Marsolier, Marie-Claude

Transcription of yeast class III genes involves the formation of a transcription initiation complex that comprises RNA polymerase III (Pol III) and the general transcription factors TFIIIB and...

A Chimeric Subunit of Yeast Transcription Factor IIIC Forms a Subcomplex with τ95

Manaud, Nathalie, Arrebola, Rosalía, Buffin-Meyer, Bénédicte, Lefebvre, Olivier, Voss, Hartmut, Riva, Michel, ...

The multisubunit yeast transcription factor IIIC (TFIIIC) is a multifunctional protein required for promoter recognition, transcription factor IIIB recruitment, and chromatin antirepression. We...

τ91, an Essential Subunit of Yeast Transcription Factor IIIC, Cooperates with τ138 in DNA Binding

Arrebola, Rosalía, Manaud, Nathalie, Rozenfeld, Sophie, Marsolier, Marie-Claude, Lefebvre, Olivier, Carles, Christophe, ...

Transcription factor IIIC (TFIIIC) (or τ) is a large multisubunit and multifunctional factor required for transcription of all class III genes in Saccharomyces cerevisiae. It is responsible for...

Multiple Roles of the τ131 Subunit of Yeast Transcription Factor IIIC (TFIIIC) in TFIIIB Assembly

Dumay-Odelot, Hélène, Acker, Joël, Arrebola, Rosalia, Sentenac, André, Marck, Christian

Yeast transcription factor IIIC (TFIIIC) plays a key role in assembling the transcription initiation factor TFIIIB on class III genes after TFIIIC-DNA binding. The second largest subunit of TFIIIC,...

The A14–A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4–Rpb7 pol II subunits

Peyroche, Gérald, Levillain, Erwann, Siaut, Magali, Callebaut, Isabelle, Schultz, Patrick, Sentenac, André, ...

A43, an essential subunit of yeast RNA polymerase I (pol I), interacts with Rrn3, a class I general transcription factor required for rDNA transcription. The pol I–Rrn3 complex is the only form of...

An Rpb4/Rpb7-Like Complex in Yeast RNA Polymerase III Contains the Orthologue of Mammalian CGRP-RCP

Siaut, Magali, Zaros, Cécile, Levivier, Emilie, Ferri, Maria-Laura, Court, Magali, Werner, Michel, ...

The essential C17 subunit of yeast RNA polymerase (Pol) III interacts with Brf1, a component of TFIIIB, suggesting a role for C17 in the initiation step of transcription. The protein sequence of C17...

Genome-wide location of yeast RNA polymerase III transcription machinery

Harismendy, Olivier, Gendrel, Christiane-Gabrielle, Soularue, Pascal, Gidrol, Xavier, Sentenac, André, Werner, Michel, ...

RNA polymerase III (Pol III) transcribes a large set of genes encoding small untranslated RNAs like tRNAs, 5S rRNA, U6 snRNA or RPR1 RNA. To get a global view of class III (Pol III-transcribed)...

The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3

Peyroche, Gérald, Milkereit, Philipp, Bischler, Nicolas, Tschochner, Herbert, Schultz, Patrick, Sentenac, André, ...

RNA polymerase I (Pol I) is dedicated to transcription of the large ribosomal DNA (rDNA). The mechanism of Pol I recruitment onto rDNA promoters is poorly understood. Here we present evidence that...

The RNA cleavage activity of RNA polymerase III is mediated by an essential TFIIS-like subunit and is important for transcription termination

Chédin, Stéphane, Riva, Michel, Schultz, Patrick, Sentenac, André, Carles, Christophe

Budding yeast RNA polymerase III (Pol III) contains a small, essential subunit, named C11, that is conserved in humans and shows a strong homology to TFIIS. A mutant Pol III, heterocomplemented with...

Modulation of Yeast Genome Expression in Response to Defective RNA Polymerase III-Dependent Transcription†

Conesa, Christine, Ruotolo, Roberta, Soularue, Pascal, Simms, Tiffany A., Donze, David, Sentenac, André, ...

We used genome-wide expression analysis in Saccharomyces cerevisiae to explore whether and how the expression of protein-coding, RNA polymerase (Pol) II-transcribed genes is influenced by a decrease...

A Subunit of Yeast TFIIIC Participates in the Recruitment of TATA-Binding Protein

Deprez, Eric, Arrebola, Rosalía, Conesa, Christine, Sentenac, André

TFIIIC plays a key role in nucleating the assembly of the initiation factor TFIIIB on class III genes. We have characterized an essential gene, TFC8, encoding the 60-kDa polypeptide, τ60, present in...

A Novel Subunit of Yeast RNA Polymerase III Interacts with the TFIIB-Related Domain of TFIIIB70

Ferri, Maria-Laura, Peyroche, Gérald, Siaut, Magali, Lefebvre, Olivier, Carles, Christophe, Conesa, Christine, ...

There is limited information on how eukaryotic RNA polymerases (Pol) recognize their cognate preinitiation complex. We have characterized a polypeptide copurifying with yeast Pol III. This protein,...

High-Mobility-Group Proteins NHP6A and NHP6B Participate in Activation of the RNA Polymerase III SNR6 Gene

Lopez, Sébastien, Livingstone-Zatchej, Magda, Jourdain, Sabine, Thoma, Fritz, Sentenac, André, Marsolier, Marie-Claude

Transcription of yeast class III genes involves the formation of a transcription initiation complex that comprises RNA polymerase III (Pol III) and the general transcription factors TFIIIB and...

A Chimeric Subunit of Yeast Transcription Factor IIIC Forms a Subcomplex with τ95

Manaud, Nathalie, Arrebola, Rosalía, Buffin-Meyer, Bénédicte, Lefebvre, Olivier, Voss, Hartmut, Riva, Michel, ...

The multisubunit yeast transcription factor IIIC (TFIIIC) is a multifunctional protein required for promoter recognition, transcription factor IIIB recruitment, and chromatin antirepression. We...

τ91, an Essential Subunit of Yeast Transcription Factor IIIC, Cooperates with τ138 in DNA Binding

Arrebola, Rosalía, Manaud, Nathalie, Rozenfeld, Sophie, Marsolier, Marie-Claude, Lefebvre, Olivier, Carles, Christophe, ...

Transcription factor IIIC (TFIIIC) (or τ) is a large multisubunit and multifunctional factor required for transcription of all class III genes in Saccharomyces cerevisiae. It is responsible for...

Multiple Roles of the τ131 Subunit of Yeast Transcription Factor IIIC (TFIIIC) in TFIIIB Assembly

Dumay-Odelot, Hélène, Acker, Joël, Arrebola, Rosalia, Sentenac, André, Marck, Christian

Yeast transcription factor IIIC (TFIIIC) plays a key role in assembling the transcription initiation factor TFIIIB on class III genes after TFIIIC-DNA binding. The second largest subunit of TFIIIC,...

The A14–A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4–Rpb7 pol II subunits

Peyroche, Gérald, Levillain, Erwann, Siaut, Magali, Callebaut, Isabelle, Schultz, Patrick, Sentenac, André, ...

A43, an essential subunit of yeast RNA polymerase I (pol I), interacts with Rrn3, a class I general transcription factor required for rDNA transcription. The pol I–Rrn3 complex is the only form of...

An Rpb4/Rpb7-Like Complex in Yeast RNA Polymerase III Contains the Orthologue of Mammalian CGRP-RCP

Siaut, Magali, Zaros, Cécile, Levivier, Emilie, Ferri, Maria-Laura, Court, Magali, Werner, Michel, ...

The essential C17 subunit of yeast RNA polymerase (Pol) III interacts with Brf1, a component of TFIIIB, suggesting a role for C17 in the initiation step of transcription. The protein sequence of C17...

Genome-wide location of yeast RNA polymerase III transcription machinery

Harismendy, Olivier, Gendrel, Christiane-Gabrielle, Soularue, Pascal, Gidrol, Xavier, Sentenac, André, Werner, Michel, ...

RNA polymerase III (Pol III) transcribes a large set of genes encoding small untranslated RNAs like tRNAs, 5S rRNA, U6 snRNA or RPR1 RNA. To get a global view of class III (Pol III-transcribed)...

The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3

Peyroche, Gérald, Milkereit, Philipp, Bischler, Nicolas, Tschochner, Herbert, Schultz, Patrick, Sentenac, André, ...

RNA polymerase I (Pol I) is dedicated to transcription of the large ribosomal DNA (rDNA). The mechanism of Pol I recruitment onto rDNA promoters is poorly understood. Here we present evidence that...

The RNA cleavage activity of RNA polymerase III is mediated by an essential TFIIS-like subunit and is important for transcription termination

Chédin, Stéphane, Riva, Michel, Schultz, Patrick, Sentenac, André, Carles, Christophe

Budding yeast RNA polymerase III (Pol III) contains a small, essential subunit, named C11, that is conserved in humans and shows a strong homology to TFIIS. A mutant Pol III, heterocomplemented with...

Modulation of Yeast Genome Expression in Response to Defective RNA Polymerase III-Dependent Transcription†

Conesa, Christine, Ruotolo, Roberta, Soularue, Pascal, Simms, Tiffany A., Donze, David, Sentenac, André, ...

We used genome-wide expression analysis in Saccharomyces cerevisiae to explore whether and how the expression of protein-coding, RNA polymerase (Pol) II-transcribed genes is influenced by a decrease...

The transcriptional activity of RNA polymerase I is a key determinant for the level of all ribosome components

Laferté, Arnaud, Favry, Emmanuel, Sentenac, André, Riva, Michel, Carles, Christophe, Chédin, Stéphane

Regulation of ribosome biogenesis is a key element of cell biology, not only because ribosomes are directly required for growth, but also because ribosome production monopolizes nearly 80% of the...