Alexandra Mant

α-Glucan, water dikinase (GWD) : A plastidic enzyme with redox-regulated and coordinated catalytic activity and binding affinity (2007)

Mikkelsen, René, Blennow, Andreas, Schürmann, Peter, Mant, Alexandra, Mutenda, Kudzai E.

The recently discovered potato tuber (Solanum tuberosum) -glucan, water dikinase (GWD) (formerly known as R1) catalyzes the phosphorylation of starch by a dikinase-type reaction mechanism in which...

α-Glucan, water dikinase (GWD) : A plastidic enzyme with redox-regulated and coordinated catalytic activity and binding affinity (2007)

Mikkelsen, René, Blennow, Andreas, Schürmann, Peter, Mant, Alexandra, Mutenda, Kudzai E.

The recently discovered potato tuber (Solanum tuberosum) -glucan, water dikinase (GWD) (formerly known as R1) catalyzes the phosphorylation of starch by a dikinase-type reaction mechanism in which...

Polymer microarrays: identification of substrates for phagocytosis assays (2006)

Mant, Alexandra, Tourniaire, Guilhem, Diaz-Mochon, Juan J., Elliott, Tim J., Williams, Anthony P., Bradley, Mark

A polymer microarray of 120 polyurethanes was used to identify polymers that promoted the adhesion of bone marrow dendritic cells (BMDC). Identified polymers were coated onto glass cover slips and...

α-glucan, water dikinase (GWD): a plastidic enzyme with redox-regulated and coordinated catalytic activity and binding affinity (2005)

Mikkelsen, René, Mutenda, Kudzai E., Mant, Alexandra, Schürmann, Peter, Blennow, Andreas

The recently discovered potato tuber (Solanum tuberosum) {alpha}-glucan, water dikinase (GWD) (formerly known as R1) catalyzes the phosphorylation of starch by a dikinase-type reaction mechanism in...

Uptake of a fluorescent dye as a swift and simple indicator of organelle intactness: import-competent chloroplasts from soil-grown Arabidopsis (2004)

Schulz, Alexander, Knoetzel, Jürgen, Scheller, Henrik V., Mant, Alexandra

We developed a rapid and reliable technique for specifically staining intact chloroplasts using the fluorescent dye carboxyfluorescein diacetate. Intact, import-competent chloroplasts were isolated...

Identification of an Arabidopsis inorganic pyrophosphatase capable of being imported into chloroplasts (2004)

Schulze, Silke, Mant, Alexandra, Kossmann, Jens, Lloyd, James R.

An Arabidopsis cDNA coding for a previously uncharacterized isoform of inorganic pyrophosphatase was isolated. It was used to complement an E. coli mutant, demonstrating that it coded for an active...

Arabidopsis VARIEGATED 3 encodes a chloroplast-targeted, zinc-finger protein required for chloroplast and palisade cell development (2004)

Næsted, Henrik, Holm, Agnethe, Jenkins, Tom, Nielsen, H. Bjørn, Harris, Cassandra A., Beale, Michael H., ...

The stable, recessive Arabidopsis variegated 3 (var3) mutant exhibits a variegated phenotype due to somatic areas lacking or containing developmentally retarded chloroplasts and greatly reduced...

Insertion of PsaK into the thylakoid membrane in a "Horseshoe" conformation occurs in the absence of signal recognition particle, nucleoside triphosphates, or functional albino3 (2001)

Mant, Alexandra, Woolhead, Cheryl A., Moore, Misty, Henry, Ralph, Robinson, Colin

The photosystem I subunit PsaK spans the thylakoid membrane twice, with the N and C termini both located in the lumen. The insertion mechanism of a thylakoid membrane protein adopting this type of...

Distinct Albino3-dependent and -independent pathways for thylakoid membrane protein insertion (2001)

Woolhead, Cheryl A., Thompson, Simon J., Moore, Misty, Tissier, Christophe, Mant, Alexandra, Rodger, Alison, ...

The homologous proteins Oxa1, YidC, and Alb3 mediate the insertion of membrane proteins in mitochondria, bacteria, and chloroplast thylakoids, respectively. Depletion of YidC in Escherichia coli...

Conformation of a purified "spontaneously" inserting thylakoid membrane protein precursor in aqueous solvent and detergent micelles (2001)

Woolhead, Cheryl A., Mant, Alexandra, Kim, Soo Jung, Robinson, Colin, Rodger, Alison

Subunit W of photosystem II (PsbW) is a single-span thylakoid membrane protein that is synthesized with a cleavable hydrophobic signal peptide and integrated into the thylakoid membrane by an...

Three Isoforms of Isoamylase Contribute Different Catalytic Properties for the Debranching of Potato GlucansW⃞

Hussain, Hasnain, Mant, Alexandra, Seale, Robert, Zeeman, Sam, Hinchliffe, Edward, Edwards, Anne, ...

Isoamylases are debranching enzymes that hydrolyze α-1,6 linkages in α-1,4/α-1,6–linked glucan polymers. In plants, they have been shown to be required for the normal synthesis of amylopectin,...

α-Glucan, water dikinase (GWD): A plastidic enzyme with redox-regulated and coordinated catalytic activity and binding affinity

Mikkelsen, René, Mutenda, Kudzai E., Mant, Alexandra, Schürmann, Peter, Blennow, Andreas

The recently discovered potato tuber (Solanum tuberosum) α-glucan, water dikinase (GWD) (formerly known as R1) catalyzes the phosphorylation of starch by a dikinase-type reaction mechanism in which...

Three Isoforms of Isoamylase Contribute Different Catalytic Properties for the Debranching of Potato GlucansW⃞

Hussain, Hasnain, Mant, Alexandra, Seale, Robert, Zeeman, Sam, Hinchliffe, Edward, Edwards, Anne, ...

Isoamylases are debranching enzymes that hydrolyze α-1,6 linkages in α-1,4/α-1,6–linked glucan polymers. In plants, they have been shown to be required for the normal synthesis of amylopectin,...

α-Glucan, water dikinase (GWD): A plastidic enzyme with redox-regulated and coordinated catalytic activity and binding affinity

Mikkelsen, René, Mutenda, Kudzai E., Mant, Alexandra, Schürmann, Peter, Blennow, Andreas

The recently discovered potato tuber (Solanum tuberosum) α-glucan, water dikinase (GWD) (formerly known as R1) catalyzes the phosphorylation of starch by a dikinase-type reaction mechanism in which...