Alain Filloux

The chaperone/usher pathways of Pseudomonas aeruginosa: Identification of fimbrial gene clusters (cup) and their involvement in biofilm formation

Vallet, Isabelle, Olson, John W., Lory, Stephen, Lazdunski, Andrée, Filloux, Alain

Pseudomonas aeruginosa, an important opportunistic human pathogen, persists in certain tissues in the form of specialized bacterial communities, referred to as biofilm. The biofilm is formed through...

Structure-Function Analysis of XcpP, a Component Involved in General Secretory Pathway-Dependent Protein Secretion in Pseudomonas aeruginosa

Bleves, Sophie, Gérard-Vincent, Manon, Lazdunski, Andrée, Filloux, Alain

The general secretory pathway of Pseudomonas aeruginosa is required for the transport of signal peptide-containing exoproteins across the cell envelope. After completion of the Sec-dependent...

Influence of Deletions within Domain II of Exotoxin A on Its Extracellular Secretion from Pseudomonas aeruginosa

Voulhoux, Romé, Taupiac, Marie-Pierre, Czjzek, Mirjam, Beaumelle, Bruno, Filloux, Alain

Pseudomonas aeruginosa is a gram-negative bacterium that secretes many proteins into the extracellular medium via the Xcp machinery. This pathway, conserved in gram-negative bacteria, is called the...

Exchange of Xcp (Gsp) Secretion Machineries between Pseudomonas aeruginosa and Pseudomonas alcaligenes: Species Specificity Unrelated to Substrate Recognition

De Groot, Arjan, Koster, Margot, Gérard-Vincent, Manon, Gerritse, Gijs, Lazdunski, Andrée, Tommassen, Jan, ...

Pseudomonas aeruginosa and Pseudomonas alcaligenes are gram-negative bacteria that secrete proteins using the type II or general secretory pathway, which requires at least 12 xcp gene products (XcpA...

Involvement of the twin-arginine translocation system in protein secretion via the type II pathway

Voulhoux, Romé, Ball, Geneviève, Ize, Bérengère, Vasil, Michael L., Lazdunski, Andrée, Wu, Long-Fei, ...

The general secretory pathway (GSP) is a two-step process for the secretion of proteins by Gram-negative bacteria. The translocation across the outer membrane is carried out by the type II system,...

Virulence factors of the human opportunistic pathogen Serratia marcescens identified by in vivo screening

Kurz, C.Léopold, Chauvet, Sophie, Andrès, Emmanuel, Aurouze, Marianne, Vallet, Isabelle, Michel, Gérard P.F., ...

The human opportunistic pathogen Serratia marcescens is a bacterium with a broad host range, and represents a growing problem for public health. Serratia marcescens kills Caenorhabditis elegans after...

Type II Protein Secretion in Pseudomonas aeruginosa: the Pseudopilus Is a Multifibrillar and Adhesive Structure

Durand, Éric, Bernadac, Alain, Ball, Geneviève, Lazdunski, Andrée, Sturgis, James N., Filloux, Alain

The type II secretion pathway of Pseudomonas aeruginosa is involved in the extracellular release of various toxins and hydrolytic enzymes such as exotoxin A and elastase. This pathway requires the...

Biofilm Formation in Pseudomonas aeruginosa: Fimbrial cup Gene Clusters Are Controlled by the Transcriptional Regulator MvaT†

Vallet, Isabelle, Diggle, Stephen P., Stacey, Rachael E., Cámara, Miguel, Ventre, Isabelle, Lory, Stephen, ...

Pseudomonas aeruginosa is an opportunistic bacterial pathogen which poses a major threat to long-term-hospitalized patients and individuals with cystic fibrosis. The capacity of P. aeruginosa to form...

Colicin A hybrids: a genetic tool for selection of type II secretion-proficient Pseudomonas strains

Voulhoux, Romé, Lazdunski, Andrée, Filloux, Alain

The Gram-negative bacterium Pseudomonas aeruginosa secretes the majority of its extracellular proteins by the type II secretion mechanism, a two-step process initiated by translocation of signal...

Quorum Sensing Negatively Controls Type III Secretion Regulon Expression in Pseudomonas aeruginosa PAO1

Bleves, Sophie, Soscia, Chantal, Nogueira-Orlandi, Patricia, Lazdunski, Andrée, Filloux, Alain

A systematic analysis of the type III secretion (T3S) genes of Pseudomonas aeruginosa strain PAO1 revealed that they are under quorum-sensing control. This observation was supported by the...

Multiple sensors control reciprocal expression of Pseudomonas aeruginosa regulatory RNA and virulence genes

Ventre, Isabelle, Goodman, Andrew L., Vallet-Gely, Isabelle, Vasseur, Perrine, Soscia, Chantal, Molin, Søren, ...

The opportunistic pathogen Pseudomonas aeruginosa is responsible for a wide range of acute and chronic infections. The transition to chronic infections is accompanied by physiological changes in the...

Pyoverdine-Mediated Iron Uptake in Pseudomonas aeruginosa: the Tat System Is Required for PvdN but Not for FpvA Transport

Voulhoux, Romé, Filloux, Alain, Schalk, Isabelle J.

Under iron-limiting conditions, Pseudomonas aeruginosa PAO1 secretes a fluorescent siderophore called pyoverdine (Pvd). After chelating iron, this ferric siderophore is transported back into the...

FppA, a Novel Pseudomonas aeruginosa Prepilin Peptidase Involved in Assembly of Type IVb Pili

De Bentzmann, Sophie, Aurouze, Marianne, Ball, Geneviève, Filloux, Alain

Several subclasses of type IV pili have been described according to the characteristics of the structural prepilin subunit. Whereas molecular mechanisms of type IVa pilus assembly have been well...

The chaperone/usher pathways of Pseudomonas aeruginosa: Identification of fimbrial gene clusters (cup) and their involvement in biofilm formation

Vallet, Isabelle, Olson, John W., Lory, Stephen, Lazdunski, Andrée, Filloux, Alain

Pseudomonas aeruginosa, an important opportunistic human pathogen, persists in certain tissues in the form of specialized bacterial communities, referred to as biofilm. The biofilm is formed through...

Structure-Function Analysis of XcpP, a Component Involved in General Secretory Pathway-Dependent Protein Secretion in Pseudomonas aeruginosa

Bleves, Sophie, Gérard-Vincent, Manon, Lazdunski, Andrée, Filloux, Alain

The general secretory pathway of Pseudomonas aeruginosa is required for the transport of signal peptide-containing exoproteins across the cell envelope. After completion of the Sec-dependent...

Influence of Deletions within Domain II of Exotoxin A on Its Extracellular Secretion from Pseudomonas aeruginosa

Voulhoux, Romé, Taupiac, Marie-Pierre, Czjzek, Mirjam, Beaumelle, Bruno, Filloux, Alain

Pseudomonas aeruginosa is a gram-negative bacterium that secretes many proteins into the extracellular medium via the Xcp machinery. This pathway, conserved in gram-negative bacteria, is called the...

Exchange of Xcp (Gsp) Secretion Machineries between Pseudomonas aeruginosa and Pseudomonas alcaligenes: Species Specificity Unrelated to Substrate Recognition

De Groot, Arjan, Koster, Margot, Gérard-Vincent, Manon, Gerritse, Gijs, Lazdunski, Andrée, Tommassen, Jan, ...

Pseudomonas aeruginosa and Pseudomonas alcaligenes are gram-negative bacteria that secrete proteins using the type II or general secretory pathway, which requires at least 12 xcp gene products (XcpA...

Involvement of the twin-arginine translocation system in protein secretion via the type II pathway

Voulhoux, Romé, Ball, Geneviève, Ize, Bérengère, Vasil, Michael L., Lazdunski, Andrée, Wu, Long-Fei, ...

The general secretory pathway (GSP) is a two-step process for the secretion of proteins by Gram-negative bacteria. The translocation across the outer membrane is carried out by the type II system,...

Virulence factors of the human opportunistic pathogen Serratia marcescens identified by in vivo screening

Kurz, C.Léopold, Chauvet, Sophie, Andrès, Emmanuel, Aurouze, Marianne, Vallet, Isabelle, Michel, Gérard P.F., ...

The human opportunistic pathogen Serratia marcescens is a bacterium with a broad host range, and represents a growing problem for public health. Serratia marcescens kills Caenorhabditis elegans after...

Type II Protein Secretion in Pseudomonas aeruginosa: the Pseudopilus Is a Multifibrillar and Adhesive Structure

Durand, Éric, Bernadac, Alain, Ball, Geneviève, Lazdunski, Andrée, Sturgis, James N., Filloux, Alain

The type II secretion pathway of Pseudomonas aeruginosa is involved in the extracellular release of various toxins and hydrolytic enzymes such as exotoxin A and elastase. This pathway requires the...

Biofilm Formation in Pseudomonas aeruginosa: Fimbrial cup Gene Clusters Are Controlled by the Transcriptional Regulator MvaT†

Vallet, Isabelle, Diggle, Stephen P., Stacey, Rachael E., Cámara, Miguel, Ventre, Isabelle, Lory, Stephen, ...

Pseudomonas aeruginosa is an opportunistic bacterial pathogen which poses a major threat to long-term-hospitalized patients and individuals with cystic fibrosis. The capacity of P. aeruginosa to form...

Colicin A hybrids: a genetic tool for selection of type II secretion-proficient Pseudomonas strains

Voulhoux, Romé, Lazdunski, Andrée, Filloux, Alain

The Gram-negative bacterium Pseudomonas aeruginosa secretes the majority of its extracellular proteins by the type II secretion mechanism, a two-step process initiated by translocation of signal...

Quorum Sensing Negatively Controls Type III Secretion Regulon Expression in Pseudomonas aeruginosa PAO1

Bleves, Sophie, Soscia, Chantal, Nogueira-Orlandi, Patricia, Lazdunski, Andrée, Filloux, Alain

A systematic analysis of the type III secretion (T3S) genes of Pseudomonas aeruginosa strain PAO1 revealed that they are under quorum-sensing control. This observation was supported by the...

Multiple sensors control reciprocal expression of Pseudomonas aeruginosa regulatory RNA and virulence genes

Ventre, Isabelle, Goodman, Andrew L., Vallet-Gely, Isabelle, Vasseur, Perrine, Soscia, Chantal, Molin, Søren, ...

The opportunistic pathogen Pseudomonas aeruginosa is responsible for a wide range of acute and chronic infections. The transition to chronic infections is accompanied by physiological changes in the...

Pyoverdine-Mediated Iron Uptake in Pseudomonas aeruginosa: the Tat System Is Required for PvdN but Not for FpvA Transport

Voulhoux, Romé, Filloux, Alain, Schalk, Isabelle J.

Under iron-limiting conditions, Pseudomonas aeruginosa PAO1 secretes a fluorescent siderophore called pyoverdine (Pvd). After chelating iron, this ferric siderophore is transported back into the...

FppA, a Novel Pseudomonas aeruginosa Prepilin Peptidase Involved in Assembly of Type IVb Pili

De Bentzmann, Sophie, Aurouze, Marianne, Ball, Geneviève, Filloux, Alain

Several subclasses of type IV pili have been described according to the characteristics of the structural prepilin subunit. Whereas molecular mechanisms of type IVa pilus assembly have been well...

Export of the Pseudopilin XcpT of the Pseudomonas aeruginosa Type II Secretion System via the Signal Recognition Particle-Sec Pathway▿

Arts, Jorik, Van Boxtel, Ria, Filloux, Alain, Tommassen, Jan, Koster, Margot

Type IV pilins and pseudopilins are found in various prokaryotic envelope protein complexes, including type IV pili and type II secretion machineries of gram-negative bacteria, competence systems of...

Cross Talk between Type III Secretion and Flagellar Assembly Systems in Pseudomonas aeruginosa▿

Soscia, Chantal, Hachani, Abderrahman, Bernadac, Alain, Filloux, Alain, Bleves, Sophie

Pseudomonas aeruginosa cytotoxicity is linked to a type III secretion system (T3SS) that delivers effectors into the host cell. We show here that a negative cross-control exists between T3SS and...

Assembly of Fimbrial Structures in Pseudomonas aeruginosa: Functionality and Specificity of Chaperone-Usher Machineries▿

Ruer, Ségolène, Stender, Silke, Filloux, Alain, De Bentzmann, Sophie

Fimbrial or nonfimbrial adhesins assembled by the bacterial chaperone-usher pathway have been demonstrated to play a key role in pathogenesis. Such an assembly mechanism has been exemplified in...

XphA/XqhA, a Novel GspCD Subunit for Type II Secretion in Pseudomonas aeruginosa▿

Michel, Gérard P. F., Durand, Eric, Filloux, Alain

The opportunistic human pathogen bacterium Pseudomonas aeruginosa secretes various exoproteins in its surrounding environment. Protein secretion involves different secretory systems, including the...

Emergence of Secretion-Defective Sublines of Pseudomonas aeruginosa PAO1 Resulting from Spontaneous Mutations in the vfr Global Regulatory Gene▿

Fox, Áine, Haas, Dieter, Reimmann, Cornelia, Heeb, Stephan, Filloux, Alain, Voulhoux, Romé

Pseudomonas aeruginosa undergoes spontaneous mutation that impairs secretion of several extracellular enzymes during extended cultivation in vitro in rich media, as well as during long-term...