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A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila

DasGupta, Ramanuj,
Nybakken, Kent,
Booker, Matthew,
Mathey-Prevot, Bernard,
Gonsalves, Foster,
Changkakoty, Binita,
Perrimon, Norbert

Abstract

A second generation dsRNA library was used to re-assess factors that influence the outcome of transcriptional reporter-based whole-genome RNAi screens for the Wnt/Wingless (wg) and Hedgehog (hh)-signaling pathways.

Publication details

Download	http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2375041
Publisher	BioMed Central
Repository	PubMed Central (PMC3 - NLM DTD) (United States)
Keywords	Method
Type	Text
Language	English