| Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus (2007) | |||||||||
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| The aerobic Gram-positive bacterium Geobacillus kaustophilus is a bacillus species that was isolated from deep-sea sediment from the Mariana Trench. 1,4-Dihydroxy-2-naphthoate (DHNA) synthetase plays a vital role in the biosynthesis of menaquinone (vitamin $K_2$) in this bacterium. DHNA synthetase from Geobacillus kaustophilus was crystallized in the orthorhombic space group $C222_1$, with unit-cell parameters a = 77.01, b = 130.66, c = 131.69 \AA. The crystal diffracted to a resolution of 2.2 \AA. Preliminary studies and molecularreplacement calculations reveal the presence of three monomers in the asymmetric unit. | |||||||||
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