| CD45 negatively regulates tumour necrosis factor and interleukin-6 production in dendritic cells | |||||||||||||
Abstract | |||||||||||||
| CD45 is known to regulate signalling through many different surface receptors in diverse haemopoietic cell types. Here we report for the first time that CD45−/− bone marrow dendritic cells (BMDC) are more activated than CD45+/+ cells and that tumour necrosis factor (TNF) and interleukin-6 (IL-6) production by BMDC and splenic dendritic cells (sDC), is increased following stimulation via Toll-like receptor (TLR)3 and TLR9. Nuclear factor-κB activation, an important downstream consequence of TLR3 and TLR9 signalling, is also increased in CD45−/− BMDC. BMDC of CD45−/− mice also produce more TNF and IL-6 following stimulation with the cytokines TNF and interferon-α. These results show that TLR signalling is increased in CD45−/− dendritic cells and imply that CD45 is a negative regulator of TLR and cytokine receptor signalling in dendritic cells. | |||||||||||||
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